Determination of immunosuppressive drugs in human urine and serum by surface-assisted laser desorption/ionization mass spectrometry with dispersive liquid-liquid microextraction
A rapid and sensitive method for the determination of immunosuppressive drugs through surface-assisted laser desorption/ionization mass spectrometric detection (SALDI/MS) was developed. Colloidal Pd and α-cyano-4-hydroxycinnamic acid (CHCA) were used as the SALDI co-matrix. To eliminate interference...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2016-01, Vol.408 (2), p.629-637 |
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| creator | Chen, Pin-Shiuan Cheng, Yu-Han Lin, Sheng-Yu Chang, Sarah Y |
| description | A rapid and sensitive method for the determination of immunosuppressive drugs through surface-assisted laser desorption/ionization mass spectrometric detection (SALDI/MS) was developed. Colloidal Pd and α-cyano-4-hydroxycinnamic acid (CHCA) were used as the SALDI co-matrix. To eliminate interference and enhance the sensitivity, dispersive liquid-liquid microextraction (DLLME) was employed to extract the immunosuppressive drugs from the aqueous solutions. Under optimal extraction and detection conditions, calibration curves for cyclosporine and everolimus in aqueous solutions were linear over a concentration range from 0.01 to 1.20 μM. For sirolimus, the linear concentration range of the calibration curve was from 0.05 to 2.00 μM. The limits of detection (LODs) were calculated to be 3, 3, and 14 nM for cyclosporine, everolimus, and sirolimus, respectively. The enrichment factors of DLLME were calculated to be 108, 122, and 101 for cyclosporine, everolimus, and sirolimus, respectively. This novel method was successfully applied for the determination of immunosuppressive drugs in human urine and serum samples. |
| doi_str_mv | 10.1007/s00216-015-9145-9 |
| format | Article |
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Colloidal Pd and α-cyano-4-hydroxycinnamic acid (CHCA) were used as the SALDI co-matrix. To eliminate interference and enhance the sensitivity, dispersive liquid-liquid microextraction (DLLME) was employed to extract the immunosuppressive drugs from the aqueous solutions. Under optimal extraction and detection conditions, calibration curves for cyclosporine and everolimus in aqueous solutions were linear over a concentration range from 0.01 to 1.20 μM. For sirolimus, the linear concentration range of the calibration curve was from 0.05 to 2.00 μM. The limits of detection (LODs) were calculated to be 3, 3, and 14 nM for cyclosporine, everolimus, and sirolimus, respectively. The enrichment factors of DLLME were calculated to be 108, 122, and 101 for cyclosporine, everolimus, and sirolimus, respectively. This novel method was successfully applied for the determination of immunosuppressive drugs in human urine and serum samples.</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-015-9145-9</identifier><identifier>PMID: 26521180</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Analysis ; Analytical Chemistry ; Aqueous solutions ; Biochemistry ; blood serum ; Calibration ; Characterization and Evaluation of Materials ; Chemical properties ; Chemistry ; Chemistry and Materials Science ; cyclosporine ; Cyclosporins ; Desorption ; detection limit ; Drugs ; Food Science ; Humans ; Immunosuppressive agents ; Immunosuppressive Agents - blood ; Immunosuppressive Agents - isolation & purification ; Immunosuppressive Agents - urine ; Ionization ; Laboratory Medicine ; Lasers ; Limit of Detection ; Liquid Phase Microextraction - methods ; liquid-phase microextraction ; Mass spectrometry ; Mathematical analysis ; Methods ; Monitoring/Environmental Analysis ; Palladium ; Research Paper ; Scientific imaging ; Serums ; Solvents ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods ; Toxicity ; Urine</subject><ispartof>Analytical and bioanalytical chemistry, 2016-01, Vol.408 (2), p.629-637</ispartof><rights>Springer-Verlag Berlin Heidelberg 2015</rights><rights>COPYRIGHT 2016 Springer</rights><rights>Springer-Verlag Berlin Heidelberg 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c636t-e90a4dafe5ceeed60f931eecadb9326339c043885af8a5b270c3033adec2c0793</citedby><cites>FETCH-LOGICAL-c636t-e90a4dafe5ceeed60f931eecadb9326339c043885af8a5b270c3033adec2c0793</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00216-015-9145-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00216-015-9145-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26521180$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Pin-Shiuan</creatorcontrib><creatorcontrib>Cheng, Yu-Han</creatorcontrib><creatorcontrib>Lin, Sheng-Yu</creatorcontrib><creatorcontrib>Chang, Sarah Y</creatorcontrib><title>Determination of immunosuppressive drugs in human urine and serum by surface-assisted laser desorption/ionization mass spectrometry with dispersive liquid-liquid microextraction</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>A rapid and sensitive method for the determination of immunosuppressive drugs through surface-assisted laser desorption/ionization mass spectrometric detection (SALDI/MS) was developed. Colloidal Pd and α-cyano-4-hydroxycinnamic acid (CHCA) were used as the SALDI co-matrix. To eliminate interference and enhance the sensitivity, dispersive liquid-liquid microextraction (DLLME) was employed to extract the immunosuppressive drugs from the aqueous solutions. Under optimal extraction and detection conditions, calibration curves for cyclosporine and everolimus in aqueous solutions were linear over a concentration range from 0.01 to 1.20 μM. For sirolimus, the linear concentration range of the calibration curve was from 0.05 to 2.00 μM. The limits of detection (LODs) were calculated to be 3, 3, and 14 nM for cyclosporine, everolimus, and sirolimus, respectively. The enrichment factors of DLLME were calculated to be 108, 122, and 101 for cyclosporine, everolimus, and sirolimus, respectively. This novel method was successfully applied for the determination of immunosuppressive drugs in human urine and serum samples.</description><subject>Analysis</subject><subject>Analytical Chemistry</subject><subject>Aqueous solutions</subject><subject>Biochemistry</subject><subject>blood serum</subject><subject>Calibration</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemical properties</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>cyclosporine</subject><subject>Cyclosporins</subject><subject>Desorption</subject><subject>detection limit</subject><subject>Drugs</subject><subject>Food Science</subject><subject>Humans</subject><subject>Immunosuppressive agents</subject><subject>Immunosuppressive Agents - blood</subject><subject>Immunosuppressive Agents - isolation & purification</subject><subject>Immunosuppressive Agents - urine</subject><subject>Ionization</subject><subject>Laboratory Medicine</subject><subject>Lasers</subject><subject>Limit of Detection</subject><subject>Liquid Phase Microextraction - methods</subject><subject>liquid-phase microextraction</subject><subject>Mass spectrometry</subject><subject>Mathematical analysis</subject><subject>Methods</subject><subject>Monitoring/Environmental Analysis</subject><subject>Palladium</subject><subject>Research Paper</subject><subject>Scientific imaging</subject><subject>Serums</subject><subject>Solvents</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</subject><subject>Toxicity</subject><subject>Urine</subject><issn>1618-2642</issn><issn>1618-2650</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkstu1TAURSMEoqXwAUzAEhMmaf2IHWdYlfKQKjGAji1f--TWVWKndly4_Sv-EIeU8hBCVZTY8ln7nHhrV9Vzgg8Jxu1RwpgSUWPC64405fOg2ieCyJoKjh_e7Ru6Vz1J6RIXUBLxuNordUqIxPvVtzcwQxyd17MLHoUeuXHMPqQ8TRFScteAbMzbhJxHF3nUHuXoPCDtLUoQ84g2O5Ry7LWBWhdBmsGiQZcaspBCnJbGR-V1N-uMsVAoTWDmGEaY4w59cfMFsq6cxR8DB3eVna3XBY3OxABf56jNon9aPer1kODZ7XpQnb89_Xzyvj77-O7DyfFZbQQTcw0d1o3VPXADAFbgvmMEwGi76RgVjHUGN0xKrnup-Ya22DDMmLZgqMFtxw6q12vfKYarDGlWo0sGhkF7CDkpUhjatA2X90BbJmnXCn4PVGApOBG4oK_-Qi9Djr7cuVCSUMK5aH5RWz2Acr4Pi1FLU3XcNFQS3PJl7OE_qPJYKPYGD70r538IyCoo3qcUoVdTdKOOO0WwWsKn1vCpkim1hE8tjr24_eG8GcHeKX6mrQB0BVIp-S3E3270n64vV1Gvg9Lb6JI6_0RxcQjjhmLJ2Xcs_PGB</recordid><startdate>20160101</startdate><enddate>20160101</enddate><creator>Chen, Pin-Shiuan</creator><creator>Cheng, Yu-Han</creator><creator>Lin, Sheng-Yu</creator><creator>Chang, Sarah Y</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>F28</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H8D</scope><scope>H8G</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KB.</scope><scope>KR7</scope><scope>L7M</scope><scope>LK8</scope><scope>L~C</scope><scope>L~D</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>20160101</creationdate><title>Determination of immunosuppressive drugs in human urine and serum by surface-assisted laser desorption/ionization mass spectrometry with dispersive liquid-liquid microextraction</title><author>Chen, Pin-Shiuan ; Cheng, Yu-Han ; Lin, Sheng-Yu ; Chang, Sarah Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c636t-e90a4dafe5ceeed60f931eecadb9326339c043885af8a5b270c3033adec2c0793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Analysis</topic><topic>Analytical Chemistry</topic><topic>Aqueous solutions</topic><topic>Biochemistry</topic><topic>blood serum</topic><topic>Calibration</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemical properties</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>cyclosporine</topic><topic>Cyclosporins</topic><topic>Desorption</topic><topic>detection limit</topic><topic>Drugs</topic><topic>Food Science</topic><topic>Humans</topic><topic>Immunosuppressive agents</topic><topic>Immunosuppressive Agents - 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Academic</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Analytical and bioanalytical chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Pin-Shiuan</au><au>Cheng, Yu-Han</au><au>Lin, Sheng-Yu</au><au>Chang, Sarah Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of immunosuppressive drugs in human urine and serum by surface-assisted laser desorption/ionization mass spectrometry with dispersive liquid-liquid microextraction</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2016-01-01</date><risdate>2016</risdate><volume>408</volume><issue>2</issue><spage>629</spage><epage>637</epage><pages>629-637</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>A rapid and sensitive method for the determination of immunosuppressive drugs through surface-assisted laser desorption/ionization mass spectrometric detection (SALDI/MS) was developed. Colloidal Pd and α-cyano-4-hydroxycinnamic acid (CHCA) were used as the SALDI co-matrix. To eliminate interference and enhance the sensitivity, dispersive liquid-liquid microextraction (DLLME) was employed to extract the immunosuppressive drugs from the aqueous solutions. Under optimal extraction and detection conditions, calibration curves for cyclosporine and everolimus in aqueous solutions were linear over a concentration range from 0.01 to 1.20 μM. For sirolimus, the linear concentration range of the calibration curve was from 0.05 to 2.00 μM. The limits of detection (LODs) were calculated to be 3, 3, and 14 nM for cyclosporine, everolimus, and sirolimus, respectively. The enrichment factors of DLLME were calculated to be 108, 122, and 101 for cyclosporine, everolimus, and sirolimus, respectively. This novel method was successfully applied for the determination of immunosuppressive drugs in human urine and serum samples.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>26521180</pmid><doi>10.1007/s00216-015-9145-9</doi><tpages>9</tpages></addata></record> |
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| subjects | Analysis Analytical Chemistry Aqueous solutions Biochemistry blood serum Calibration Characterization and Evaluation of Materials Chemical properties Chemistry Chemistry and Materials Science cyclosporine Cyclosporins Desorption detection limit Drugs Food Science Humans Immunosuppressive agents Immunosuppressive Agents - blood Immunosuppressive Agents - isolation & purification Immunosuppressive Agents - urine Ionization Laboratory Medicine Lasers Limit of Detection Liquid Phase Microextraction - methods liquid-phase microextraction Mass spectrometry Mathematical analysis Methods Monitoring/Environmental Analysis Palladium Research Paper Scientific imaging Serums Solvents Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Toxicity Urine |
| title | Determination of immunosuppressive drugs in human urine and serum by surface-assisted laser desorption/ionization mass spectrometry with dispersive liquid-liquid microextraction |
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