Tris-acetate polyacrylamide gradient gel electrophoresis for the analysis of protein oligomerization

Here we report a new approach for studying protein oligomerization in cells using a single electrophoresis gel. We combined the use of a crosslinking reagent for sample preparation, such as glutaraldehyde, with the analysis of oligomers by Tris-acetate polyacrylamide gel electrophoresis. The use of...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Analytical and bioanalytical chemistry 2016-02, Vol.408 (6), p.1715-1719
Hauptverfasser:	Cubillos-Rojas, Monica, Schneider, Taiane, Sánchez-Tena, Susana, Bartrons, Ramon, Ventura, Francesc, Rosa, Jose Luis
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetates - chemistry Analysis Analytical Chemistry Antibodies Biochemistry Bleomycin - pharmacology Cell Line Characterization and Evaluation of Materials Chemical properties Chemistry Chemistry and Materials Science Cross-Linking Reagents - chemistry Crosslinking Doxorubicin - pharmacology Electrophoresis Electrophoresis, Polyacrylamide Gel - methods Extrapolation Food Science Gel electrophoresis gels Glutaral - chemistry glutaraldehyde Humans Kinases Laboratory Medicine Molecular Weight Monitoring/Environmental Analysis Oligomerization Oligomers polyacrylamide polyacrylamide gel electrophoresis Polyacrylamides Polymer crosslinking Protein Multimerization - drug effects Proteins Proteins - analysis Proteins - metabolism Reagents Sample preparation Tumor proteins Tumor Suppressor Protein p53 - analysis Tumor Suppressor Protein p53 - metabolism
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!