A novel adenoviral vector expressing human Fas/CD95/APO-1 enhances p53-mediated apoptosis
Recent evidence suggests an intriguing link between p53 and the Fas pathway. To evaluate this association further, we utilized a recombinant adenoviral vector (AdWTp53) to overexpress wild-type p53 in lung cancer (A549, H23, EKVX and HOP92) and breast cancer (MDA-MB-231 and MCF-7) cell lines and obs...
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| Veröffentlicht in: | Cell death and differentiation 1999-04, Vol.6 (4), p.326-333 |
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| container_title | Cell death and differentiation |
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| creator | Rakkar, A N Katayose, Y Kim, M Craig, C Ohri, E Li, Z Cowan, K H Seth, P |
| description | Recent evidence suggests an intriguing link between p53 and the Fas pathway. To evaluate this association further, we utilized a recombinant adenoviral vector (AdWTp53) to overexpress wild-type p53 in lung cancer (A549, H23, EKVX and HOP92) and breast cancer (MDA-MB-231 and MCF-7) cell lines and observed an increase in the Fas/CD95/APO-1 protein levels. Furthermore, this increase correlated with the sensitivity of the cell lines to p53-mediated cytotoxicity. To examine the effects of Fas over-expression in cells resistant to p53 over-expression, we constructed AdFas, an adenoviral vector capable of transferring functional human Fas to cancer cells. Interestingly, infection of p53-resistant MCF-7 cells with AdFas sensitized them to p53-mediated apoptosis. These studies indicate that combined over-expression of Fas and wild-type p53 may be an effective cancer gene therapy approach, especially in cells relatively resistant to p53 over-expression. |
| doi_str_mv | 10.1038/sj.cdd.4400498 |
| format | Article |
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These studies indicate that combined over-expression of Fas and wild-type p53 may be an effective cancer gene therapy approach, especially in cells relatively resistant to p53 over-expression.</description><subject>Adenoviridae</subject><subject>Adenoviridae Infections</subject><subject>APO-1 protein</subject><subject>Apoptosis - genetics</subject><subject>Breast Neoplasms</subject><subject>CD95 antigen</subject><subject>Cytotoxins - genetics</subject><subject>DNA Primers</subject><subject>DNA, Neoplasm - analysis</subject><subject>Fas antigen</subject><subject>fas Receptor - genetics</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Genetic Therapy - methods</subject><subject>Genetic Vectors</subject><subject>human adenovirus</subject><subject>Humans</subject><subject>Lung Neoplasms</subject><subject>Tumor Cells, Cultured - chemistry</subject><subject>Tumor Cells, Cultured - cytology</subject><subject>Tumor Cells, Cultured - virology</subject><subject>Tumor Suppressor Protein p53 - physiology</subject><issn>1350-9047</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kDtPwzAUhT2AaCmsjMgTW1K713F8x6pQQKpUBhiYIr9KU-Vh4qSCf08QZTpn-HT06RByw1nKGah5PKTWuVQIxgSqMzLlkLEEmcgn5DLGA2NM5igvyOQX53KRTcn7kjbt0VdUOz-WstMVPXrbtx31X6HzMZbNB90PtW7oWsf56h6z-fJlm3Dqm71urI80ZJDU3pW6947q0Ia-jWW8Iuc7XUV_fcoZeVs_vK6eks328Xm13CSB56xPJHLp7cJwQABtpUNmUQoDyhqbCZOPriAUoDbMKgsSUOxwoZXgCEY7mJG7v93QtZ-Dj31Rl9H6qtKNb4dY8ByBYaZG8PYEDmbULUJX1rr7Lv7PgB-vrl4i</recordid><startdate>199904</startdate><enddate>199904</enddate><creator>Rakkar, A N</creator><creator>Katayose, Y</creator><creator>Kim, M</creator><creator>Craig, C</creator><creator>Ohri, E</creator><creator>Li, Z</creator><creator>Cowan, K H</creator><creator>Seth, P</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7TO</scope><scope>H94</scope></search><sort><creationdate>199904</creationdate><title>A novel adenoviral vector expressing human Fas/CD95/APO-1 enhances p53-mediated apoptosis</title><author>Rakkar, A N ; Katayose, Y ; Kim, M ; Craig, C ; Ohri, E ; Li, Z ; Cowan, K H ; Seth, P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p170t-6916ec2b13933ac6d90c964b38cbc54b710334839ab0c8c36394f92a84193bad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Adenoviridae</topic><topic>Adenoviridae Infections</topic><topic>APO-1 protein</topic><topic>Apoptosis - genetics</topic><topic>Breast Neoplasms</topic><topic>CD95 antigen</topic><topic>Cytotoxins - genetics</topic><topic>DNA Primers</topic><topic>DNA, Neoplasm - analysis</topic><topic>Fas antigen</topic><topic>fas Receptor - genetics</topic><topic>Female</topic><topic>Gene Expression</topic><topic>Genetic Therapy - methods</topic><topic>Genetic Vectors</topic><topic>human adenovirus</topic><topic>Humans</topic><topic>Lung Neoplasms</topic><topic>Tumor Cells, Cultured - chemistry</topic><topic>Tumor Cells, Cultured - cytology</topic><topic>Tumor Cells, Cultured - virology</topic><topic>Tumor Suppressor Protein p53 - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rakkar, A N</creatorcontrib><creatorcontrib>Katayose, Y</creatorcontrib><creatorcontrib>Kim, M</creatorcontrib><creatorcontrib>Craig, C</creatorcontrib><creatorcontrib>Ohri, E</creatorcontrib><creatorcontrib>Li, Z</creatorcontrib><creatorcontrib>Cowan, K H</creatorcontrib><creatorcontrib>Seth, P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Cell death and differentiation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rakkar, A N</au><au>Katayose, Y</au><au>Kim, M</au><au>Craig, C</au><au>Ohri, E</au><au>Li, Z</au><au>Cowan, K H</au><au>Seth, P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel adenoviral vector expressing human Fas/CD95/APO-1 enhances p53-mediated apoptosis</atitle><jtitle>Cell death and differentiation</jtitle><addtitle>Cell Death Differ</addtitle><date>1999-04</date><risdate>1999</risdate><volume>6</volume><issue>4</issue><spage>326</spage><epage>333</epage><pages>326-333</pages><issn>1350-9047</issn><abstract>Recent evidence suggests an intriguing link between p53 and the Fas pathway. 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| source | MEDLINE; SpringerNature Journals; EZB-FREE-00999 freely available EZB journals |
| subjects | Adenoviridae Adenoviridae Infections APO-1 protein Apoptosis - genetics Breast Neoplasms CD95 antigen Cytotoxins - genetics DNA Primers DNA, Neoplasm - analysis Fas antigen fas Receptor - genetics Female Gene Expression Genetic Therapy - methods Genetic Vectors human adenovirus Humans Lung Neoplasms Tumor Cells, Cultured - chemistry Tumor Cells, Cultured - cytology Tumor Cells, Cultured - virology Tumor Suppressor Protein p53 - physiology |
| title | A novel adenoviral vector expressing human Fas/CD95/APO-1 enhances p53-mediated apoptosis |
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