Role of conserved residues in the activity of adenovirus preterminal protein

Centre for Biomolecular Sciences, School of Biology, University of St Andrews, Biomolecular Sciences Building, North Haugh, St Andrews, Fife KY16 9ST, UK 1 Author for correspondence: Ronald Hay. Fax +44 1334 462595. e-mail rth{at}st-andrews.ac.uk Preterminal protein (pTP) is a component of the prein...

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Veröffentlicht in:Journal of general virology 2001-08, Vol.82 (8), p.1917-1927
Hauptverfasser: Botting, Catherine H, Hay, Ronald T
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container_end_page 1927
container_issue 8
container_start_page 1917
container_title Journal of general virology
container_volume 82
creator Botting, Catherine H
Hay, Ronald T
description Centre for Biomolecular Sciences, School of Biology, University of St Andrews, Biomolecular Sciences Building, North Haugh, St Andrews, Fife KY16 9ST, UK 1 Author for correspondence: Ronald Hay. Fax +44 1334 462595. e-mail rth{at}st-andrews.ac.uk Preterminal protein (pTP) is a component of the preinitiation complex which forms at the adenovirus origin of DNA replication and acts as the protein primer during DNA synthesis. In order to determine the role of various regions of the molecule a series of 18 mutations was introduced into conserved motifs of pTP which were predicted to be surface exposed, and the mutants expressed in insect cells using a baculovirus expression system. Their ability to initiate DNA replication was assessed and the effect the mutations have on the individual interactions which contribute to the formation of the pre-initiation complex was determined. Classes of mutants could be identified which were unable to bind DNA or interact with the adenovirus DNA polymerase, but one class of mutants retained these activities and yet failed to initiate DNA replication. These mutants therefore identify regions of pTP required for different aspects of adenovirus DNA replication.
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source MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection
subjects Adenovirus
Adenoviruses, Human
Amino Acid Sequence
Animals
Baculoviridae - genetics
Baculovirus
Cell Line
DNA Replication
DNA, Single-Stranded
DNA, Viral - biosynthesis
Genetic Vectors
Humans
Molecular Sequence Data
Mutagenesis, Site-Directed
Phosphoproteins - genetics
Phosphoproteins - physiology
Protein Binding
Protein Precursors - genetics
Protein Precursors - physiology
terminal protein
Viral Proteins
Virus Replication
title Role of conserved residues in the activity of adenovirus preterminal protein
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