Triptolide induced cytotoxic effects on human promyelocytic leukemia, T cell lymphoma and human hepatocellular carcinoma cell lines
Triptolide, a traditional Chinese medicine, has been reported to be effective in the treatment of auto-immune diseases, and it can also induce anti-neoplastic activity on several human tumor cell lines. This study investigates the cytotoxic function and the functional mechanism of triptolide on tumo...
Gespeichert in:
| Veröffentlicht in: | Toxicology letters 2001-05, Vol.122 (1), p.81-87 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 87 |
|---|---|
| container_issue | 1 |
| container_start_page | 81 |
| container_title | Toxicology letters |
| container_volume | 122 |
| creator | Wai-Ching Chan, Ella Chak-Sum Cheng, Samuel Wan-Yee Sin, Fion Xie, Yong |
| description | Triptolide, a traditional Chinese medicine, has been reported to be effective in the treatment of auto-immune diseases, and it can also induce anti-neoplastic activity on several human tumor cell lines. This study investigates the cytotoxic function and the functional mechanism of triptolide on tumor cells. Promyelocytic leukemia, (HL-60), T cell lymphoma (Jurkat), and human hepatocelluar carcinoma (SMMC-7721) cells were subjected to triptolide treatment, and cell growth inhibition was examined by XTT cell viability assay. Cell death mechanism (apoptosis) was confirmed through DNA fragmentation and DAPI staining. Triptolide inhibited 50% of cell growth (IC
50) on HL-60 cells at 7.5 nM, Jurkat cells at 27.5 nM and SMMC cells at 32 nM. Characteristic apoptotic features including internucleosomal DNA fragmentation and chromatin condensation were observed in triptolide treated cells. Data from the study indicates that triptolide could induce apoptosis in human tumor cell lines and it may be applicable as a potential chemotherapeutic agent for cancer treatment. |
| doi_str_mv | 10.1016/S0378-4274(01)00353-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_17925860</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0378427401003538</els_id><sourcerecordid>17925860</sourcerecordid><originalsourceid>FETCH-LOGICAL-c421t-cc35f2fe0d6696deb8272273d70f728a7f2387743f6468bb939f228c65e9579a3</originalsourceid><addsrcrecordid>eNqFkcuO1DAQRS0EYpqBTwB5gRBIBPyIX6sRGvGSRmJBs7bcTlltSOJgJ4he8-M40xGwY-VFnVuuOoXQY0peUULl68-EK920TLXPCX1BCBe80XfQjmplGk6luYt2f5AL9KCUr4QQ2UpxH11Qyo0SwuzQr32O05z62AGOY7d46LA_zWlOP6PHEAL4ueA04uMyuBFPOQ0n6FNFarmH5RsM0b3Ee-yh73F_GqZjGhx2Y7cljjC5Oa3VpXcZe5d9HFfkHIgjlIfoXnB9gUfbe4m-vHu7v_7Q3Hx6__H6zU3jW0bnxnsuAgtAOimN7OCgmWJM8U6RoJh2KjCulWp5qFvqw8FwExjTXgowQhnHL9Gzc9-6xfcFymyHWNYx3AhpKZYqw4SWpILiDPqcSskQ7JTj4PLJUmJX-_bWvl3VWkLtrX2ra-7J9sFyGKD7m9p0V-DpBrjiXR-yG30s_3RvqVSsYldnDKqNHxGyLT7CWG8Tc72H7VL8zyS_AcKno0E</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17925860</pqid></control><display><type>article</type><title>Triptolide induced cytotoxic effects on human promyelocytic leukemia, T cell lymphoma and human hepatocellular carcinoma cell lines</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Wai-Ching Chan, Ella ; Chak-Sum Cheng, Samuel ; Wan-Yee Sin, Fion ; Xie, Yong</creator><creatorcontrib>Wai-Ching Chan, Ella ; Chak-Sum Cheng, Samuel ; Wan-Yee Sin, Fion ; Xie, Yong</creatorcontrib><description>Triptolide, a traditional Chinese medicine, has been reported to be effective in the treatment of auto-immune diseases, and it can also induce anti-neoplastic activity on several human tumor cell lines. This study investigates the cytotoxic function and the functional mechanism of triptolide on tumor cells. Promyelocytic leukemia, (HL-60), T cell lymphoma (Jurkat), and human hepatocelluar carcinoma (SMMC-7721) cells were subjected to triptolide treatment, and cell growth inhibition was examined by XTT cell viability assay. Cell death mechanism (apoptosis) was confirmed through DNA fragmentation and DAPI staining. Triptolide inhibited 50% of cell growth (IC
50) on HL-60 cells at 7.5 nM, Jurkat cells at 27.5 nM and SMMC cells at 32 nM. Characteristic apoptotic features including internucleosomal DNA fragmentation and chromatin condensation were observed in triptolide treated cells. Data from the study indicates that triptolide could induce apoptosis in human tumor cell lines and it may be applicable as a potential chemotherapeutic agent for cancer treatment.</description><identifier>ISSN: 0378-4274</identifier><identifier>EISSN: 1879-3169</identifier><identifier>DOI: 10.1016/S0378-4274(01)00353-8</identifier><identifier>PMID: 11397559</identifier><identifier>CODEN: TOLED5</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Antineoplastic agents ; Antineoplastic Agents, Alkylating - pharmacology ; Apoptosis ; Biological and medical sciences ; Cell Survival - drug effects ; Cytotoxicity ; Diterpenes - pharmacology ; Diterpenoid triepoxide ; DNA Fragmentation - drug effects ; Dose-Response Relationship, Drug ; Epoxy Compounds ; General aspects ; HL-60 Cells ; Humans ; Immuno-suppression ; Immunomodulators ; Indoles ; Jurkat Cells ; Medical sciences ; Microscopy, Fluorescence ; Pharmacology. Drug treatments ; Phenanthrenes ; Staining and Labeling ; Triptolide ; Tumor Cells, Cultured</subject><ispartof>Toxicology letters, 2001-05, Vol.122 (1), p.81-87</ispartof><rights>2001 Elsevier Science Ireland Ltd</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c421t-cc35f2fe0d6696deb8272273d70f728a7f2387743f6468bb939f228c65e9579a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378427401003538$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1041672$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11397559$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wai-Ching Chan, Ella</creatorcontrib><creatorcontrib>Chak-Sum Cheng, Samuel</creatorcontrib><creatorcontrib>Wan-Yee Sin, Fion</creatorcontrib><creatorcontrib>Xie, Yong</creatorcontrib><title>Triptolide induced cytotoxic effects on human promyelocytic leukemia, T cell lymphoma and human hepatocellular carcinoma cell lines</title><title>Toxicology letters</title><addtitle>Toxicol Lett</addtitle><description>Triptolide, a traditional Chinese medicine, has been reported to be effective in the treatment of auto-immune diseases, and it can also induce anti-neoplastic activity on several human tumor cell lines. This study investigates the cytotoxic function and the functional mechanism of triptolide on tumor cells. Promyelocytic leukemia, (HL-60), T cell lymphoma (Jurkat), and human hepatocelluar carcinoma (SMMC-7721) cells were subjected to triptolide treatment, and cell growth inhibition was examined by XTT cell viability assay. Cell death mechanism (apoptosis) was confirmed through DNA fragmentation and DAPI staining. Triptolide inhibited 50% of cell growth (IC
50) on HL-60 cells at 7.5 nM, Jurkat cells at 27.5 nM and SMMC cells at 32 nM. Characteristic apoptotic features including internucleosomal DNA fragmentation and chromatin condensation were observed in triptolide treated cells. Data from the study indicates that triptolide could induce apoptosis in human tumor cell lines and it may be applicable as a potential chemotherapeutic agent for cancer treatment.</description><subject>Antineoplastic agents</subject><subject>Antineoplastic Agents, Alkylating - pharmacology</subject><subject>Apoptosis</subject><subject>Biological and medical sciences</subject><subject>Cell Survival - drug effects</subject><subject>Cytotoxicity</subject><subject>Diterpenes - pharmacology</subject><subject>Diterpenoid triepoxide</subject><subject>DNA Fragmentation - drug effects</subject><subject>Dose-Response Relationship, Drug</subject><subject>Epoxy Compounds</subject><subject>General aspects</subject><subject>HL-60 Cells</subject><subject>Humans</subject><subject>Immuno-suppression</subject><subject>Immunomodulators</subject><subject>Indoles</subject><subject>Jurkat Cells</subject><subject>Medical sciences</subject><subject>Microscopy, Fluorescence</subject><subject>Pharmacology. Drug treatments</subject><subject>Phenanthrenes</subject><subject>Staining and Labeling</subject><subject>Triptolide</subject><subject>Tumor Cells, Cultured</subject><issn>0378-4274</issn><issn>1879-3169</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcuO1DAQRS0EYpqBTwB5gRBIBPyIX6sRGvGSRmJBs7bcTlltSOJgJ4he8-M40xGwY-VFnVuuOoXQY0peUULl68-EK920TLXPCX1BCBe80XfQjmplGk6luYt2f5AL9KCUr4QQ2UpxH11Qyo0SwuzQr32O05z62AGOY7d46LA_zWlOP6PHEAL4ueA04uMyuBFPOQ0n6FNFarmH5RsM0b3Ee-yh73F_GqZjGhx2Y7cljjC5Oa3VpXcZe5d9HFfkHIgjlIfoXnB9gUfbe4m-vHu7v_7Q3Hx6__H6zU3jW0bnxnsuAgtAOimN7OCgmWJM8U6RoJh2KjCulWp5qFvqw8FwExjTXgowQhnHL9Gzc9-6xfcFymyHWNYx3AhpKZYqw4SWpILiDPqcSskQ7JTj4PLJUmJX-_bWvl3VWkLtrX2ra-7J9sFyGKD7m9p0V-DpBrjiXR-yG30s_3RvqVSsYldnDKqNHxGyLT7CWG8Tc72H7VL8zyS_AcKno0E</recordid><startdate>20010531</startdate><enddate>20010531</enddate><creator>Wai-Ching Chan, Ella</creator><creator>Chak-Sum Cheng, Samuel</creator><creator>Wan-Yee Sin, Fion</creator><creator>Xie, Yong</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20010531</creationdate><title>Triptolide induced cytotoxic effects on human promyelocytic leukemia, T cell lymphoma and human hepatocellular carcinoma cell lines</title><author>Wai-Ching Chan, Ella ; Chak-Sum Cheng, Samuel ; Wan-Yee Sin, Fion ; Xie, Yong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c421t-cc35f2fe0d6696deb8272273d70f728a7f2387743f6468bb939f228c65e9579a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Antineoplastic agents</topic><topic>Antineoplastic Agents, Alkylating - pharmacology</topic><topic>Apoptosis</topic><topic>Biological and medical sciences</topic><topic>Cell Survival - drug effects</topic><topic>Cytotoxicity</topic><topic>Diterpenes - pharmacology</topic><topic>Diterpenoid triepoxide</topic><topic>DNA Fragmentation - drug effects</topic><topic>Dose-Response Relationship, Drug</topic><topic>Epoxy Compounds</topic><topic>General aspects</topic><topic>HL-60 Cells</topic><topic>Humans</topic><topic>Immuno-suppression</topic><topic>Immunomodulators</topic><topic>Indoles</topic><topic>Jurkat Cells</topic><topic>Medical sciences</topic><topic>Microscopy, Fluorescence</topic><topic>Pharmacology. Drug treatments</topic><topic>Phenanthrenes</topic><topic>Staining and Labeling</topic><topic>Triptolide</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wai-Ching Chan, Ella</creatorcontrib><creatorcontrib>Chak-Sum Cheng, Samuel</creatorcontrib><creatorcontrib>Wan-Yee Sin, Fion</creatorcontrib><creatorcontrib>Xie, Yong</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Toxicology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wai-Ching Chan, Ella</au><au>Chak-Sum Cheng, Samuel</au><au>Wan-Yee Sin, Fion</au><au>Xie, Yong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Triptolide induced cytotoxic effects on human promyelocytic leukemia, T cell lymphoma and human hepatocellular carcinoma cell lines</atitle><jtitle>Toxicology letters</jtitle><addtitle>Toxicol Lett</addtitle><date>2001-05-31</date><risdate>2001</risdate><volume>122</volume><issue>1</issue><spage>81</spage><epage>87</epage><pages>81-87</pages><issn>0378-4274</issn><eissn>1879-3169</eissn><coden>TOLED5</coden><abstract>Triptolide, a traditional Chinese medicine, has been reported to be effective in the treatment of auto-immune diseases, and it can also induce anti-neoplastic activity on several human tumor cell lines. This study investigates the cytotoxic function and the functional mechanism of triptolide on tumor cells. Promyelocytic leukemia, (HL-60), T cell lymphoma (Jurkat), and human hepatocelluar carcinoma (SMMC-7721) cells were subjected to triptolide treatment, and cell growth inhibition was examined by XTT cell viability assay. Cell death mechanism (apoptosis) was confirmed through DNA fragmentation and DAPI staining. Triptolide inhibited 50% of cell growth (IC
50) on HL-60 cells at 7.5 nM, Jurkat cells at 27.5 nM and SMMC cells at 32 nM. Characteristic apoptotic features including internucleosomal DNA fragmentation and chromatin condensation were observed in triptolide treated cells. Data from the study indicates that triptolide could induce apoptosis in human tumor cell lines and it may be applicable as a potential chemotherapeutic agent for cancer treatment.</abstract><cop>Shannon</cop><cop>Amsterdam</cop><pub>Elsevier Ireland Ltd</pub><pmid>11397559</pmid><doi>10.1016/S0378-4274(01)00353-8</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-4274 |
| ispartof | Toxicology letters, 2001-05, Vol.122 (1), p.81-87 |
| issn | 0378-4274 1879-3169 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_17925860 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Antineoplastic agents Antineoplastic Agents, Alkylating - pharmacology Apoptosis Biological and medical sciences Cell Survival - drug effects Cytotoxicity Diterpenes - pharmacology Diterpenoid triepoxide DNA Fragmentation - drug effects Dose-Response Relationship, Drug Epoxy Compounds General aspects HL-60 Cells Humans Immuno-suppression Immunomodulators Indoles Jurkat Cells Medical sciences Microscopy, Fluorescence Pharmacology. Drug treatments Phenanthrenes Staining and Labeling Triptolide Tumor Cells, Cultured |
| title | Triptolide induced cytotoxic effects on human promyelocytic leukemia, T cell lymphoma and human hepatocellular carcinoma cell lines |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-21T20%3A20%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Triptolide%20induced%20cytotoxic%20effects%20on%20human%20promyelocytic%20leukemia,%20T%20cell%20lymphoma%20and%20human%20hepatocellular%20carcinoma%20cell%20lines&rft.jtitle=Toxicology%20letters&rft.au=Wai-Ching%20Chan,%20Ella&rft.date=2001-05-31&rft.volume=122&rft.issue=1&rft.spage=81&rft.epage=87&rft.pages=81-87&rft.issn=0378-4274&rft.eissn=1879-3169&rft.coden=TOLED5&rft_id=info:doi/10.1016/S0378-4274(01)00353-8&rft_dat=%3Cproquest_cross%3E17925860%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17925860&rft_id=info:pmid/11397559&rft_els_id=S0378427401003538&rfr_iscdi=true |