Elevated levels of circulating CD4+CRTh2+ T cells characterize severe asthma

Summary Background Chemoattractant receptor‐homologous molecule expressed on Th2 cells (CRTh2) is a receptor for PGD2 and expressed by T cells, eosinophils, basophils, and ILC2 cells. CRTh2 expression by CD4+ T cells identifies the Th2 subset, and these cells have been characterized as allergen‐spec...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Clinical and experimental allergy 2016-06, Vol.46 (6), p.825-836
Hauptverfasser: Palikhe, N. S., Laratta, C., Nahirney, D., Vethanayagam, D., Bhutani, M., Vliagoftis, H., Cameron, L.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 836
container_issue 6
container_start_page 825
container_title Clinical and experimental allergy
container_volume 46
creator Palikhe, N. S.
Laratta, C.
Nahirney, D.
Vethanayagam, D.
Bhutani, M.
Vliagoftis, H.
Cameron, L.
description Summary Background Chemoattractant receptor‐homologous molecule expressed on Th2 cells (CRTh2) is a receptor for PGD2 and expressed by T cells, eosinophils, basophils, and ILC2 cells. CRTh2 expression by CD4+ T cells identifies the Th2 subset, and these cells have been characterized as allergen‐specific central memory Th2 cells. Recently, activation of the PGD2‐CRTh2 pathway in the lungs was associated with severe asthma. Objective To assess circulating levels of Th2 cells and related mediators in severe asthma and those who experience asthma exacerbations. Methods Peripheral blood cells expressing CRTh2 were characterized by flow cytometry and qRT‐PCR. Serum IL‐13 and PGD2 were measured by ELISA and compared with asthma severity and tendency to exacerbate. Results Severe asthmatics had more circulating CD4+CRTh2+ T cells, CRTh2 and GATA3 mRNA, and a higher level of serum IL‐13 compared to mild/moderate asthmatics. The proportion of CD4+CRTh2+ T cells was associated with lower lung function and was highest in severe asthmatics that exacerbated in the last year. Circulating CD4+CRTh2+ T cells, unlike eosinophils, were positively correlated with inhaled steroid dose. Conclusions and Clinical Relevance Elevated levels of circulating CD4+CRTh2+ T cells are a feature of severe asthma, despite high‐dose corticosteroids. Tracking the systemic level of these cells may help identify type 2 severe asthmatics at risk of exacerbation.
doi_str_mv 10.1111/cea.12741
format Article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_1792378698</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>4067836031</sourcerecordid><originalsourceid>FETCH-LOGICAL-i2471-f7e317fdba56bbc988bd7ec4475ab2bfc2fc83bc10e03631561103d337f10eae3</originalsourceid><addsrcrecordid>eNpdkU1PwzAMhiMEYmNw4A-gSlyQpm5J0zbtEZUxPiqQpiGOUZq6LKNdR9IC49eTfbADvtiKn9dy_CJ0TvCA2BhKEAPiMZ8coC6hYeB6Ng5RF8eB77Io9jvoxJg5xpgGcXSMOh7DLPbiqIvSUQmfooHcsRlK49SFI5WWbSkatXhzkhu_n0ymM6_vTB0JpSXkTGghG9DqBxxjVRocYZpZJU7RUSFKA2e73EMvt6Npcuemz-P75Dp1lecz4hYMKGFFnokgzDIZR1GWM5C-zwKReVkhvUJGNJMEA6YhJUFICKY5paywTwJoD11t5y51_dGCaXilzHo5sYC6NZzYz1EWhXFk0ct_6Lxu9cJut6YItTfZUBc7qs0qyPlSq0roFf-7kwWGW-BLlbDa9wnmawO4NYBvDODJ6HpTWIW7VSjTwPdeIfQ7DxllAX99GnP_YRI8ppMxZ_QXxnuEug</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1791300098</pqid></control><display><type>article</type><title>Elevated levels of circulating CD4+CRTh2+ T cells characterize severe asthma</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><creator>Palikhe, N. S. ; Laratta, C. ; Nahirney, D. ; Vethanayagam, D. ; Bhutani, M. ; Vliagoftis, H. ; Cameron, L.</creator><creatorcontrib>Palikhe, N. S. ; Laratta, C. ; Nahirney, D. ; Vethanayagam, D. ; Bhutani, M. ; Vliagoftis, H. ; Cameron, L.</creatorcontrib><description>Summary Background Chemoattractant receptor‐homologous molecule expressed on Th2 cells (CRTh2) is a receptor for PGD2 and expressed by T cells, eosinophils, basophils, and ILC2 cells. CRTh2 expression by CD4+ T cells identifies the Th2 subset, and these cells have been characterized as allergen‐specific central memory Th2 cells. Recently, activation of the PGD2‐CRTh2 pathway in the lungs was associated with severe asthma. Objective To assess circulating levels of Th2 cells and related mediators in severe asthma and those who experience asthma exacerbations. Methods Peripheral blood cells expressing CRTh2 were characterized by flow cytometry and qRT‐PCR. Serum IL‐13 and PGD2 were measured by ELISA and compared with asthma severity and tendency to exacerbate. Results Severe asthmatics had more circulating CD4+CRTh2+ T cells, CRTh2 and GATA3 mRNA, and a higher level of serum IL‐13 compared to mild/moderate asthmatics. The proportion of CD4+CRTh2+ T cells was associated with lower lung function and was highest in severe asthmatics that exacerbated in the last year. Circulating CD4+CRTh2+ T cells, unlike eosinophils, were positively correlated with inhaled steroid dose. Conclusions and Clinical Relevance Elevated levels of circulating CD4+CRTh2+ T cells are a feature of severe asthma, despite high‐dose corticosteroids. Tracking the systemic level of these cells may help identify type 2 severe asthmatics at risk of exacerbation.</description><identifier>ISSN: 0954-7894</identifier><identifier>EISSN: 1365-2222</identifier><identifier>DOI: 10.1111/cea.12741</identifier><identifier>PMID: 27079298</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Adrenal Cortex Hormones - therapeutic use ; Adult ; Animals ; Asthma ; Asthma - blood ; Asthma - diagnosis ; Asthma - immunology ; Asthma - metabolism ; biomarker ; Biomarkers ; CD4+CRTh2+ T cells ; CRTh2 ; Cytokines - blood ; Cytokines - metabolism ; Disease Models, Animal ; Disease Progression ; exacerbation ; Gene Expression Profiling ; glucocorticosteroid responsiveness ; Humans ; Immunoglobulin E - blood ; Immunoglobulin E - immunology ; Immunophenotyping ; Inflammation Mediators - blood ; Inflammation Mediators - metabolism ; Lymphocyte Activation - immunology ; Lymphocyte Count ; Lymphocytes ; Mice ; Middle Aged ; PGD 2 ; PGD2 ; Phenotype ; Respiratory Function Tests ; Risk Factors ; severe asthma ; Severity of Illness Index ; Signal Transduction ; T-Lymphocyte Subsets - immunology ; T-Lymphocyte Subsets - metabolism ; Th2 cells ; Th2 Cells - immunology ; Th2 Cells - metabolism ; type 2 immunity</subject><ispartof>Clinical and experimental allergy, 2016-06, Vol.46 (6), p.825-836</ispartof><rights>2016 John Wiley &amp; Sons Ltd</rights><rights>2016 John Wiley &amp; Sons Ltd.</rights><rights>Copyright © 2016 John Wiley &amp; Sons Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fcea.12741$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fcea.12741$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27079298$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Palikhe, N. S.</creatorcontrib><creatorcontrib>Laratta, C.</creatorcontrib><creatorcontrib>Nahirney, D.</creatorcontrib><creatorcontrib>Vethanayagam, D.</creatorcontrib><creatorcontrib>Bhutani, M.</creatorcontrib><creatorcontrib>Vliagoftis, H.</creatorcontrib><creatorcontrib>Cameron, L.</creatorcontrib><title>Elevated levels of circulating CD4+CRTh2+ T cells characterize severe asthma</title><title>Clinical and experimental allergy</title><addtitle>Clin Exp Allergy</addtitle><description>Summary Background Chemoattractant receptor‐homologous molecule expressed on Th2 cells (CRTh2) is a receptor for PGD2 and expressed by T cells, eosinophils, basophils, and ILC2 cells. CRTh2 expression by CD4+ T cells identifies the Th2 subset, and these cells have been characterized as allergen‐specific central memory Th2 cells. Recently, activation of the PGD2‐CRTh2 pathway in the lungs was associated with severe asthma. Objective To assess circulating levels of Th2 cells and related mediators in severe asthma and those who experience asthma exacerbations. Methods Peripheral blood cells expressing CRTh2 were characterized by flow cytometry and qRT‐PCR. Serum IL‐13 and PGD2 were measured by ELISA and compared with asthma severity and tendency to exacerbate. Results Severe asthmatics had more circulating CD4+CRTh2+ T cells, CRTh2 and GATA3 mRNA, and a higher level of serum IL‐13 compared to mild/moderate asthmatics. The proportion of CD4+CRTh2+ T cells was associated with lower lung function and was highest in severe asthmatics that exacerbated in the last year. Circulating CD4+CRTh2+ T cells, unlike eosinophils, were positively correlated with inhaled steroid dose. Conclusions and Clinical Relevance Elevated levels of circulating CD4+CRTh2+ T cells are a feature of severe asthma, despite high‐dose corticosteroids. Tracking the systemic level of these cells may help identify type 2 severe asthmatics at risk of exacerbation.</description><subject>Adrenal Cortex Hormones - therapeutic use</subject><subject>Adult</subject><subject>Animals</subject><subject>Asthma</subject><subject>Asthma - blood</subject><subject>Asthma - diagnosis</subject><subject>Asthma - immunology</subject><subject>Asthma - metabolism</subject><subject>biomarker</subject><subject>Biomarkers</subject><subject>CD4+CRTh2+ T cells</subject><subject>CRTh2</subject><subject>Cytokines - blood</subject><subject>Cytokines - metabolism</subject><subject>Disease Models, Animal</subject><subject>Disease Progression</subject><subject>exacerbation</subject><subject>Gene Expression Profiling</subject><subject>glucocorticosteroid responsiveness</subject><subject>Humans</subject><subject>Immunoglobulin E - blood</subject><subject>Immunoglobulin E - immunology</subject><subject>Immunophenotyping</subject><subject>Inflammation Mediators - blood</subject><subject>Inflammation Mediators - metabolism</subject><subject>Lymphocyte Activation - immunology</subject><subject>Lymphocyte Count</subject><subject>Lymphocytes</subject><subject>Mice</subject><subject>Middle Aged</subject><subject>PGD 2</subject><subject>PGD2</subject><subject>Phenotype</subject><subject>Respiratory Function Tests</subject><subject>Risk Factors</subject><subject>severe asthma</subject><subject>Severity of Illness Index</subject><subject>Signal Transduction</subject><subject>T-Lymphocyte Subsets - immunology</subject><subject>T-Lymphocyte Subsets - metabolism</subject><subject>Th2 cells</subject><subject>Th2 Cells - immunology</subject><subject>Th2 Cells - metabolism</subject><subject>type 2 immunity</subject><issn>0954-7894</issn><issn>1365-2222</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkU1PwzAMhiMEYmNw4A-gSlyQpm5J0zbtEZUxPiqQpiGOUZq6LKNdR9IC49eTfbADvtiKn9dy_CJ0TvCA2BhKEAPiMZ8coC6hYeB6Ng5RF8eB77Io9jvoxJg5xpgGcXSMOh7DLPbiqIvSUQmfooHcsRlK49SFI5WWbSkatXhzkhu_n0ymM6_vTB0JpSXkTGghG9DqBxxjVRocYZpZJU7RUSFKA2e73EMvt6Npcuemz-P75Dp1lecz4hYMKGFFnokgzDIZR1GWM5C-zwKReVkhvUJGNJMEA6YhJUFICKY5paywTwJoD11t5y51_dGCaXilzHo5sYC6NZzYz1EWhXFk0ct_6Lxu9cJut6YItTfZUBc7qs0qyPlSq0roFf-7kwWGW-BLlbDa9wnmawO4NYBvDODJ6HpTWIW7VSjTwPdeIfQ7DxllAX99GnP_YRI8ppMxZ_QXxnuEug</recordid><startdate>201606</startdate><enddate>201606</enddate><creator>Palikhe, N. S.</creator><creator>Laratta, C.</creator><creator>Nahirney, D.</creator><creator>Vethanayagam, D.</creator><creator>Bhutani, M.</creator><creator>Vliagoftis, H.</creator><creator>Cameron, L.</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7T5</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>201606</creationdate><title>Elevated levels of circulating CD4+CRTh2+ T cells characterize severe asthma</title><author>Palikhe, N. S. ; Laratta, C. ; Nahirney, D. ; Vethanayagam, D. ; Bhutani, M. ; Vliagoftis, H. ; Cameron, L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i2471-f7e317fdba56bbc988bd7ec4475ab2bfc2fc83bc10e03631561103d337f10eae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adrenal Cortex Hormones - therapeutic use</topic><topic>Adult</topic><topic>Animals</topic><topic>Asthma</topic><topic>Asthma - blood</topic><topic>Asthma - diagnosis</topic><topic>Asthma - immunology</topic><topic>Asthma - metabolism</topic><topic>biomarker</topic><topic>Biomarkers</topic><topic>CD4+CRTh2+ T cells</topic><topic>CRTh2</topic><topic>Cytokines - blood</topic><topic>Cytokines - metabolism</topic><topic>Disease Models, Animal</topic><topic>Disease Progression</topic><topic>exacerbation</topic><topic>Gene Expression Profiling</topic><topic>glucocorticosteroid responsiveness</topic><topic>Humans</topic><topic>Immunoglobulin E - blood</topic><topic>Immunoglobulin E - immunology</topic><topic>Immunophenotyping</topic><topic>Inflammation Mediators - blood</topic><topic>Inflammation Mediators - metabolism</topic><topic>Lymphocyte Activation - immunology</topic><topic>Lymphocyte Count</topic><topic>Lymphocytes</topic><topic>Mice</topic><topic>Middle Aged</topic><topic>PGD 2</topic><topic>PGD2</topic><topic>Phenotype</topic><topic>Respiratory Function Tests</topic><topic>Risk Factors</topic><topic>severe asthma</topic><topic>Severity of Illness Index</topic><topic>Signal Transduction</topic><topic>T-Lymphocyte Subsets - immunology</topic><topic>T-Lymphocyte Subsets - metabolism</topic><topic>Th2 cells</topic><topic>Th2 Cells - immunology</topic><topic>Th2 Cells - metabolism</topic><topic>type 2 immunity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Palikhe, N. S.</creatorcontrib><creatorcontrib>Laratta, C.</creatorcontrib><creatorcontrib>Nahirney, D.</creatorcontrib><creatorcontrib>Vethanayagam, D.</creatorcontrib><creatorcontrib>Bhutani, M.</creatorcontrib><creatorcontrib>Vliagoftis, H.</creatorcontrib><creatorcontrib>Cameron, L.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical and experimental allergy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Palikhe, N. S.</au><au>Laratta, C.</au><au>Nahirney, D.</au><au>Vethanayagam, D.</au><au>Bhutani, M.</au><au>Vliagoftis, H.</au><au>Cameron, L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Elevated levels of circulating CD4+CRTh2+ T cells characterize severe asthma</atitle><jtitle>Clinical and experimental allergy</jtitle><addtitle>Clin Exp Allergy</addtitle><date>2016-06</date><risdate>2016</risdate><volume>46</volume><issue>6</issue><spage>825</spage><epage>836</epage><pages>825-836</pages><issn>0954-7894</issn><eissn>1365-2222</eissn><abstract>Summary Background Chemoattractant receptor‐homologous molecule expressed on Th2 cells (CRTh2) is a receptor for PGD2 and expressed by T cells, eosinophils, basophils, and ILC2 cells. CRTh2 expression by CD4+ T cells identifies the Th2 subset, and these cells have been characterized as allergen‐specific central memory Th2 cells. Recently, activation of the PGD2‐CRTh2 pathway in the lungs was associated with severe asthma. Objective To assess circulating levels of Th2 cells and related mediators in severe asthma and those who experience asthma exacerbations. Methods Peripheral blood cells expressing CRTh2 were characterized by flow cytometry and qRT‐PCR. Serum IL‐13 and PGD2 were measured by ELISA and compared with asthma severity and tendency to exacerbate. Results Severe asthmatics had more circulating CD4+CRTh2+ T cells, CRTh2 and GATA3 mRNA, and a higher level of serum IL‐13 compared to mild/moderate asthmatics. The proportion of CD4+CRTh2+ T cells was associated with lower lung function and was highest in severe asthmatics that exacerbated in the last year. Circulating CD4+CRTh2+ T cells, unlike eosinophils, were positively correlated with inhaled steroid dose. Conclusions and Clinical Relevance Elevated levels of circulating CD4+CRTh2+ T cells are a feature of severe asthma, despite high‐dose corticosteroids. Tracking the systemic level of these cells may help identify type 2 severe asthmatics at risk of exacerbation.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>27079298</pmid><doi>10.1111/cea.12741</doi><tpages>12</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0954-7894
ispartof Clinical and experimental allergy, 2016-06, Vol.46 (6), p.825-836
issn 0954-7894
1365-2222
language eng
recordid cdi_proquest_miscellaneous_1792378698
source MEDLINE; Access via Wiley Online Library
subjects Adrenal Cortex Hormones - therapeutic use
Adult
Animals
Asthma
Asthma - blood
Asthma - diagnosis
Asthma - immunology
Asthma - metabolism
biomarker
Biomarkers
CD4+CRTh2+ T cells
CRTh2
Cytokines - blood
Cytokines - metabolism
Disease Models, Animal
Disease Progression
exacerbation
Gene Expression Profiling
glucocorticosteroid responsiveness
Humans
Immunoglobulin E - blood
Immunoglobulin E - immunology
Immunophenotyping
Inflammation Mediators - blood
Inflammation Mediators - metabolism
Lymphocyte Activation - immunology
Lymphocyte Count
Lymphocytes
Mice
Middle Aged
PGD 2
PGD2
Phenotype
Respiratory Function Tests
Risk Factors
severe asthma
Severity of Illness Index
Signal Transduction
T-Lymphocyte Subsets - immunology
T-Lymphocyte Subsets - metabolism
Th2 cells
Th2 Cells - immunology
Th2 Cells - metabolism
type 2 immunity
title Elevated levels of circulating CD4+CRTh2+ T cells characterize severe asthma
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T19%3A02%3A10IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Elevated%20levels%20of%20circulating%20CD4+CRTh2+%20T%20cells%20characterize%20severe%20asthma&rft.jtitle=Clinical%20and%20experimental%20allergy&rft.au=Palikhe,%20N.%20S.&rft.date=2016-06&rft.volume=46&rft.issue=6&rft.spage=825&rft.epage=836&rft.pages=825-836&rft.issn=0954-7894&rft.eissn=1365-2222&rft_id=info:doi/10.1111/cea.12741&rft_dat=%3Cproquest_pubme%3E4067836031%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1791300098&rft_id=info:pmid/27079298&rfr_iscdi=true