Anti-arthritic activity of 11-O-(4′-O-methyl galloyl)-bergenin and Crassula capitella extract in rats
Objectives Isolation and identification of phytochemicals of Crassula capitella (Thunberg), evaluation of the anti‐arthritic potential of the extract and the major isolated compound; 11‐O‐(4′‐O‐methyl galloyl)‐bergenin and underlying their mechanism on rat model of rheumatoid arthritis (RA). Methods...
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| Veröffentlicht in: | Journal of pharmacy and pharmacology 2016-06, Vol.68 (6), p.834-844 |
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| creator | El-Hawary, Seham S. Mohammed, Rabab Abouzid, Sameh Ali, Zeinab Y. Elwekeel, Ahlam |
| description | Objectives
Isolation and identification of phytochemicals of Crassula capitella (Thunberg), evaluation of the anti‐arthritic potential of the extract and the major isolated compound; 11‐O‐(4′‐O‐methyl galloyl)‐bergenin and underlying their mechanism on rat model of rheumatoid arthritis (RA).
Methods
Different fractions were subjected to column chromatography giving fourteen compound identified by mass and NMR spectroscopic techniques. RA was induced by intraplantar injection of complete Freund's adjuvant into the right hind paw of rats. Influence of tested samples in comparable to methotrexate on paw oedema, body weight gain, serum diagnostic markers, cartilage and bone degeneration enzymes, pro‐inflammatory mediators and oxidative stress biomarkers in arthritic rats.
Key findings
Fourteen phenolic compounds were isolated and identified for the first time from C. capitella. The major compound identified as 11‐O‐(4′‐O‐methyl galloyl)‐bergenin. Treatment of arthritic rats with extract or 11‐O‐(4′‐O‐methyl galloyl)‐bergenin with the tested doses can reduce the progression and severity of RA.
Conclusion
Crassula capitella is a new natural and abundant source for 11‐O‐(4′‐O‐methyl galloyl)‐bergenin for resolving chronic inflammatory diseases as RA through antioxidant, anti‐inflammatory and membrane stabilizing mechanism. |
| doi_str_mv | 10.1111/jphp.12566 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1792370445</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>4069178661</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3956-ddd1d14c9936c167edd33cb1171f87aea731ed1c09dcd1266d88587998bef83a3</originalsourceid><addsrcrecordid>eNp90MFu1DAQBmALgei2cOEBUCQuLZKLx47t5FitoKWqaCWKKvVieW1n10s2CbYDzY1n4pF4Erxs2wMHfBkfvvk1-hF6BeQY8nu3HlbDMVAuxBM0o6SkWAKvnqIZIZRixiXbQ_sxrgkhUgjxHO1RSRkBVs7Q8qRLHuuQVsEnbwptkv_u01T0TQGAL_Fh-fvnrzw3Lq2mtljqtu2n9ggvXFi6zneF7mwxDzrGsdWF0YNPrs0_d5dCDiuyCDrFF-hZo9voXt7PA_Tlw_vr-Rm-uDz9OD-5wIbVXGBrLVgoTV0zYUBIZy1jZgEgoamkdloycBYMqa2xQIWwVcUrWdfVwjUV0-wAHe5yh9B_G11MauOj2V7UuX6MCmRNmSRlyTN98w9d92Po8nVbBbyknJZZvd0pE_oYg2vUEPxGh0kBUdv61bZ-9bf-jF_fR46LjbOP9KHvDGAHfvjWTf-JUudXZ1cPoXi342Nyd487OnxVQjLJ1c2nU3VzK8_r22umPrM_0H2e-Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1791542524</pqid></control><display><type>article</type><title>Anti-arthritic activity of 11-O-(4′-O-methyl galloyl)-bergenin and Crassula capitella extract in rats</title><source>MEDLINE</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Wiley Online Library All Journals</source><creator>El-Hawary, Seham S. ; Mohammed, Rabab ; Abouzid, Sameh ; Ali, Zeinab Y. ; Elwekeel, Ahlam</creator><creatorcontrib>El-Hawary, Seham S. ; Mohammed, Rabab ; Abouzid, Sameh ; Ali, Zeinab Y. ; Elwekeel, Ahlam</creatorcontrib><description>Objectives
Isolation and identification of phytochemicals of Crassula capitella (Thunberg), evaluation of the anti‐arthritic potential of the extract and the major isolated compound; 11‐O‐(4′‐O‐methyl galloyl)‐bergenin and underlying their mechanism on rat model of rheumatoid arthritis (RA).
Methods
Different fractions were subjected to column chromatography giving fourteen compound identified by mass and NMR spectroscopic techniques. RA was induced by intraplantar injection of complete Freund's adjuvant into the right hind paw of rats. Influence of tested samples in comparable to methotrexate on paw oedema, body weight gain, serum diagnostic markers, cartilage and bone degeneration enzymes, pro‐inflammatory mediators and oxidative stress biomarkers in arthritic rats.
Key findings
Fourteen phenolic compounds were isolated and identified for the first time from C. capitella. The major compound identified as 11‐O‐(4′‐O‐methyl galloyl)‐bergenin. Treatment of arthritic rats with extract or 11‐O‐(4′‐O‐methyl galloyl)‐bergenin with the tested doses can reduce the progression and severity of RA.
Conclusion
Crassula capitella is a new natural and abundant source for 11‐O‐(4′‐O‐methyl galloyl)‐bergenin for resolving chronic inflammatory diseases as RA through antioxidant, anti‐inflammatory and membrane stabilizing mechanism.</description><identifier>ISSN: 0022-3573</identifier><identifier>EISSN: 2042-7158</identifier><identifier>DOI: 10.1111/jphp.12566</identifier><identifier>PMID: 27230134</identifier><identifier>CODEN: JPPMAB</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>11-O-(4′-O-methyl galloyl)-bergenin ; Animals ; Arthritis, Experimental - blood ; Arthritis, Experimental - chemically induced ; Arthritis, Experimental - pathology ; Arthritis, Experimental - prevention & control ; Benzopyrans - isolation & purification ; Benzopyrans - pharmacology ; Biomarkers - blood ; Bone and Bones - drug effects ; Bone and Bones - enzymology ; Carbon-13 Magnetic Resonance Spectroscopy ; Cartilage, Articular - drug effects ; Cartilage, Articular - enzymology ; Chromatography, High Pressure Liquid ; Crassula capitella ; Crassulaceae - chemistry ; Cytokines - blood ; Disease Progression ; Egypt ; Female ; Freund's Adjuvant ; Gallic Acid - analogs & derivatives ; Gallic Acid - isolation & purification ; Gallic Acid - pharmacology ; Inflammation Mediators - blood ; Joints - drug effects ; Joints - enzymology ; Joints - pathology ; lysosomal enzymes ; Mass Spectrometry ; Oxidative Stress - drug effects ; Phytotherapy ; Plant Components, Aerial ; Plant Extracts - isolation & purification ; Plant Extracts - pharmacology ; Plants, Medicinal ; pro-inflammatory mediators ; Proton Magnetic Resonance Spectroscopy ; Rats, Sprague-Dawley ; rheumatoid arthritis ; Severity of Illness Index ; Time Factors ; Weight Gain</subject><ispartof>Journal of pharmacy and pharmacology, 2016-06, Vol.68 (6), p.834-844</ispartof><rights>2016 Royal Pharmaceutical Society</rights><rights>2016 Royal Pharmaceutical Society.</rights><rights>Copyright © 2016 Royal Pharmaceutical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3956-ddd1d14c9936c167edd33cb1171f87aea731ed1c09dcd1266d88587998bef83a3</citedby><cites>FETCH-LOGICAL-c3956-ddd1d14c9936c167edd33cb1171f87aea731ed1c09dcd1266d88587998bef83a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjphp.12566$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjphp.12566$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27230134$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>El-Hawary, Seham S.</creatorcontrib><creatorcontrib>Mohammed, Rabab</creatorcontrib><creatorcontrib>Abouzid, Sameh</creatorcontrib><creatorcontrib>Ali, Zeinab Y.</creatorcontrib><creatorcontrib>Elwekeel, Ahlam</creatorcontrib><title>Anti-arthritic activity of 11-O-(4′-O-methyl galloyl)-bergenin and Crassula capitella extract in rats</title><title>Journal of pharmacy and pharmacology</title><addtitle>J Pharm Pharmacol</addtitle><description>Objectives
Isolation and identification of phytochemicals of Crassula capitella (Thunberg), evaluation of the anti‐arthritic potential of the extract and the major isolated compound; 11‐O‐(4′‐O‐methyl galloyl)‐bergenin and underlying their mechanism on rat model of rheumatoid arthritis (RA).
Methods
Different fractions were subjected to column chromatography giving fourteen compound identified by mass and NMR spectroscopic techniques. RA was induced by intraplantar injection of complete Freund's adjuvant into the right hind paw of rats. Influence of tested samples in comparable to methotrexate on paw oedema, body weight gain, serum diagnostic markers, cartilage and bone degeneration enzymes, pro‐inflammatory mediators and oxidative stress biomarkers in arthritic rats.
Key findings
Fourteen phenolic compounds were isolated and identified for the first time from C. capitella. The major compound identified as 11‐O‐(4′‐O‐methyl galloyl)‐bergenin. Treatment of arthritic rats with extract or 11‐O‐(4′‐O‐methyl galloyl)‐bergenin with the tested doses can reduce the progression and severity of RA.
Conclusion
Crassula capitella is a new natural and abundant source for 11‐O‐(4′‐O‐methyl galloyl)‐bergenin for resolving chronic inflammatory diseases as RA through antioxidant, anti‐inflammatory and membrane stabilizing mechanism.</description><subject>11-O-(4′-O-methyl galloyl)-bergenin</subject><subject>Animals</subject><subject>Arthritis, Experimental - blood</subject><subject>Arthritis, Experimental - chemically induced</subject><subject>Arthritis, Experimental - pathology</subject><subject>Arthritis, Experimental - prevention & control</subject><subject>Benzopyrans - isolation & purification</subject><subject>Benzopyrans - pharmacology</subject><subject>Biomarkers - blood</subject><subject>Bone and Bones - drug effects</subject><subject>Bone and Bones - enzymology</subject><subject>Carbon-13 Magnetic Resonance Spectroscopy</subject><subject>Cartilage, Articular - drug effects</subject><subject>Cartilage, Articular - enzymology</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Crassula capitella</subject><subject>Crassulaceae - chemistry</subject><subject>Cytokines - blood</subject><subject>Disease Progression</subject><subject>Egypt</subject><subject>Female</subject><subject>Freund's Adjuvant</subject><subject>Gallic Acid - analogs & derivatives</subject><subject>Gallic Acid - isolation & purification</subject><subject>Gallic Acid - pharmacology</subject><subject>Inflammation Mediators - blood</subject><subject>Joints - drug effects</subject><subject>Joints - enzymology</subject><subject>Joints - pathology</subject><subject>lysosomal enzymes</subject><subject>Mass Spectrometry</subject><subject>Oxidative Stress - drug effects</subject><subject>Phytotherapy</subject><subject>Plant Components, Aerial</subject><subject>Plant Extracts - isolation & purification</subject><subject>Plant Extracts - pharmacology</subject><subject>Plants, Medicinal</subject><subject>pro-inflammatory mediators</subject><subject>Proton Magnetic Resonance Spectroscopy</subject><subject>Rats, Sprague-Dawley</subject><subject>rheumatoid arthritis</subject><subject>Severity of Illness Index</subject><subject>Time Factors</subject><subject>Weight Gain</subject><issn>0022-3573</issn><issn>2042-7158</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90MFu1DAQBmALgei2cOEBUCQuLZKLx47t5FitoKWqaCWKKvVieW1n10s2CbYDzY1n4pF4Erxs2wMHfBkfvvk1-hF6BeQY8nu3HlbDMVAuxBM0o6SkWAKvnqIZIZRixiXbQ_sxrgkhUgjxHO1RSRkBVs7Q8qRLHuuQVsEnbwptkv_u01T0TQGAL_Fh-fvnrzw3Lq2mtljqtu2n9ggvXFi6zneF7mwxDzrGsdWF0YNPrs0_d5dCDiuyCDrFF-hZo9voXt7PA_Tlw_vr-Rm-uDz9OD-5wIbVXGBrLVgoTV0zYUBIZy1jZgEgoamkdloycBYMqa2xQIWwVcUrWdfVwjUV0-wAHe5yh9B_G11MauOj2V7UuX6MCmRNmSRlyTN98w9d92Po8nVbBbyknJZZvd0pE_oYg2vUEPxGh0kBUdv61bZ-9bf-jF_fR46LjbOP9KHvDGAHfvjWTf-JUudXZ1cPoXi342Nyd487OnxVQjLJ1c2nU3VzK8_r22umPrM_0H2e-Q</recordid><startdate>201606</startdate><enddate>201606</enddate><creator>El-Hawary, Seham S.</creator><creator>Mohammed, Rabab</creator><creator>Abouzid, Sameh</creator><creator>Ali, Zeinab Y.</creator><creator>Elwekeel, Ahlam</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TK</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>201606</creationdate><title>Anti-arthritic activity of 11-O-(4′-O-methyl galloyl)-bergenin and Crassula capitella extract in rats</title><author>El-Hawary, Seham S. ; Mohammed, Rabab ; Abouzid, Sameh ; Ali, Zeinab Y. ; Elwekeel, Ahlam</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3956-ddd1d14c9936c167edd33cb1171f87aea731ed1c09dcd1266d88587998bef83a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>11-O-(4′-O-methyl galloyl)-bergenin</topic><topic>Animals</topic><topic>Arthritis, Experimental - blood</topic><topic>Arthritis, Experimental - chemically induced</topic><topic>Arthritis, Experimental - pathology</topic><topic>Arthritis, Experimental - prevention & control</topic><topic>Benzopyrans - isolation & purification</topic><topic>Benzopyrans - pharmacology</topic><topic>Biomarkers - blood</topic><topic>Bone and Bones - drug effects</topic><topic>Bone and Bones - enzymology</topic><topic>Carbon-13 Magnetic Resonance Spectroscopy</topic><topic>Cartilage, Articular - drug effects</topic><topic>Cartilage, Articular - enzymology</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Crassula capitella</topic><topic>Crassulaceae - chemistry</topic><topic>Cytokines - blood</topic><topic>Disease Progression</topic><topic>Egypt</topic><topic>Female</topic><topic>Freund's Adjuvant</topic><topic>Gallic Acid - analogs & derivatives</topic><topic>Gallic Acid - isolation & purification</topic><topic>Gallic Acid - pharmacology</topic><topic>Inflammation Mediators - blood</topic><topic>Joints - drug effects</topic><topic>Joints - enzymology</topic><topic>Joints - pathology</topic><topic>lysosomal enzymes</topic><topic>Mass Spectrometry</topic><topic>Oxidative Stress - drug effects</topic><topic>Phytotherapy</topic><topic>Plant Components, Aerial</topic><topic>Plant Extracts - isolation & purification</topic><topic>Plant Extracts - pharmacology</topic><topic>Plants, Medicinal</topic><topic>pro-inflammatory mediators</topic><topic>Proton Magnetic Resonance Spectroscopy</topic><topic>Rats, Sprague-Dawley</topic><topic>rheumatoid arthritis</topic><topic>Severity of Illness Index</topic><topic>Time Factors</topic><topic>Weight Gain</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>El-Hawary, Seham S.</creatorcontrib><creatorcontrib>Mohammed, Rabab</creatorcontrib><creatorcontrib>Abouzid, Sameh</creatorcontrib><creatorcontrib>Ali, Zeinab Y.</creatorcontrib><creatorcontrib>Elwekeel, Ahlam</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmacy and pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>El-Hawary, Seham S.</au><au>Mohammed, Rabab</au><au>Abouzid, Sameh</au><au>Ali, Zeinab Y.</au><au>Elwekeel, Ahlam</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anti-arthritic activity of 11-O-(4′-O-methyl galloyl)-bergenin and Crassula capitella extract in rats</atitle><jtitle>Journal of pharmacy and pharmacology</jtitle><addtitle>J Pharm Pharmacol</addtitle><date>2016-06</date><risdate>2016</risdate><volume>68</volume><issue>6</issue><spage>834</spage><epage>844</epage><pages>834-844</pages><issn>0022-3573</issn><eissn>2042-7158</eissn><coden>JPPMAB</coden><abstract>Objectives
Isolation and identification of phytochemicals of Crassula capitella (Thunberg), evaluation of the anti‐arthritic potential of the extract and the major isolated compound; 11‐O‐(4′‐O‐methyl galloyl)‐bergenin and underlying their mechanism on rat model of rheumatoid arthritis (RA).
Methods
Different fractions were subjected to column chromatography giving fourteen compound identified by mass and NMR spectroscopic techniques. RA was induced by intraplantar injection of complete Freund's adjuvant into the right hind paw of rats. Influence of tested samples in comparable to methotrexate on paw oedema, body weight gain, serum diagnostic markers, cartilage and bone degeneration enzymes, pro‐inflammatory mediators and oxidative stress biomarkers in arthritic rats.
Key findings
Fourteen phenolic compounds were isolated and identified for the first time from C. capitella. The major compound identified as 11‐O‐(4′‐O‐methyl galloyl)‐bergenin. Treatment of arthritic rats with extract or 11‐O‐(4′‐O‐methyl galloyl)‐bergenin with the tested doses can reduce the progression and severity of RA.
Conclusion
Crassula capitella is a new natural and abundant source for 11‐O‐(4′‐O‐methyl galloyl)‐bergenin for resolving chronic inflammatory diseases as RA through antioxidant, anti‐inflammatory and membrane stabilizing mechanism.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>27230134</pmid><doi>10.1111/jphp.12566</doi><tpages>11</tpages></addata></record> |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); Wiley Online Library All Journals |
| subjects | 11-O-(4′-O-methyl galloyl)-bergenin Animals Arthritis, Experimental - blood Arthritis, Experimental - chemically induced Arthritis, Experimental - pathology Arthritis, Experimental - prevention & control Benzopyrans - isolation & purification Benzopyrans - pharmacology Biomarkers - blood Bone and Bones - drug effects Bone and Bones - enzymology Carbon-13 Magnetic Resonance Spectroscopy Cartilage, Articular - drug effects Cartilage, Articular - enzymology Chromatography, High Pressure Liquid Crassula capitella Crassulaceae - chemistry Cytokines - blood Disease Progression Egypt Female Freund's Adjuvant Gallic Acid - analogs & derivatives Gallic Acid - isolation & purification Gallic Acid - pharmacology Inflammation Mediators - blood Joints - drug effects Joints - enzymology Joints - pathology lysosomal enzymes Mass Spectrometry Oxidative Stress - drug effects Phytotherapy Plant Components, Aerial Plant Extracts - isolation & purification Plant Extracts - pharmacology Plants, Medicinal pro-inflammatory mediators Proton Magnetic Resonance Spectroscopy Rats, Sprague-Dawley rheumatoid arthritis Severity of Illness Index Time Factors Weight Gain |
| title | Anti-arthritic activity of 11-O-(4′-O-methyl galloyl)-bergenin and Crassula capitella extract in rats |
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