Detection of the phytotoxin coronatine by ELISA and localization in infected plant tissue
Several pathovars of Pseudomonas syringae produce the phytotoxin coronatine (COR)in vitro, and the availability of purified toxin has facilitated development of immunological detection methods. A modified, indirect competitive ELISA using the COR-specific monoclonal antibody 11B8 was developed to de...
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| Veröffentlicht in: | Physiological and molecular plant pathology 2001-06, Vol.58 (6), p.247-258 |
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| container_title | Physiological and molecular plant pathology |
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| creator | Zhao, Y.F. Jones, W.T. Sutherland, P. Palmer, D.A. Mitchell, R.E. Reynolds, P.H.S. Damicone, J.P. Bender, C.L. |
| description | Several pathovars of Pseudomonas syringae produce the phytotoxin coronatine (COR)in vitro, and the availability of purified toxin has facilitated development of immunological detection methods. A modified, indirect competitive ELISA using the COR-specific monoclonal antibody 11B8 was developed to detect COR in various host plants infected by P. syringae. The estimated detection limit for COR was 50pg per well, and COR could be reliably quantified from 5 to 40ngml−1. The subcellular localization of COR within infected tomato tissue was investigated using the COR-specific antibody MAb 8H3G2. Immunofluorescence microscopy and immunogold labelling showed that COR was present inside tomato cells and was associated with chloroplasts and particles of proteinase inhibitor I. Localization studies indicated that COR is mobile in infected plant tissue and can be detected in healthy tissue adjacent to the bacterial lesions. |
| doi_str_mv | 10.1006/pmpp.2001.0334 |
| format | Article |
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A modified, indirect competitive ELISA using the COR-specific monoclonal antibody 11B8 was developed to detect COR in various host plants infected by P. syringae. The estimated detection limit for COR was 50pg per well, and COR could be reliably quantified from 5 to 40ngml−1. The subcellular localization of COR within infected tomato tissue was investigated using the COR-specific antibody MAb 8H3G2. Immunofluorescence microscopy and immunogold labelling showed that COR was present inside tomato cells and was associated with chloroplasts and particles of proteinase inhibitor I. Localization studies indicated that COR is mobile in infected plant tissue and can be detected in healthy tissue adjacent to the bacterial lesions.</description><identifier>ISSN: 0885-5765</identifier><identifier>EISSN: 1096-1178</identifier><identifier>DOI: 10.1006/pmpp.2001.0334</identifier><identifier>CODEN: PMPPEZ</identifier><language>eng</language><publisher>London: Elsevier India Pvt Ltd</publisher><subject>Bacterial plant pathogens ; Biological and medical sciences ; Brassica ; cauliflower ; collard ; coronatine ; Fundamental and applied biological sciences. Psychology ; Generalities. Techniques. Transmission, epidemiology, ecology. Antibacterial substances, control ; Glycinemax ; Lycopersicon esculentum ; Phytopathology. Animal pests. Plant and forest protection ; Pseudomonas syringae ; soybean ; tomato</subject><ispartof>Physiological and molecular plant pathology, 2001-06, Vol.58 (6), p.247-258</ispartof><rights>2001 Academic Press</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c347t-a935bdfee263d48930e5796534e606e45aac88b17928081ad56bed16fdb99f023</citedby><cites>FETCH-LOGICAL-c347t-a935bdfee263d48930e5796534e606e45aac88b17928081ad56bed16fdb99f023</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/pmpp.2001.0334$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14439492$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Y.F.</creatorcontrib><creatorcontrib>Jones, W.T.</creatorcontrib><creatorcontrib>Sutherland, P.</creatorcontrib><creatorcontrib>Palmer, D.A.</creatorcontrib><creatorcontrib>Mitchell, R.E.</creatorcontrib><creatorcontrib>Reynolds, P.H.S.</creatorcontrib><creatorcontrib>Damicone, J.P.</creatorcontrib><creatorcontrib>Bender, C.L.</creatorcontrib><title>Detection of the phytotoxin coronatine by ELISA and localization in infected plant tissue</title><title>Physiological and molecular plant pathology</title><description>Several pathovars of Pseudomonas syringae produce the phytotoxin coronatine (COR)in vitro, and the availability of purified toxin has facilitated development of immunological detection methods. A modified, indirect competitive ELISA using the COR-specific monoclonal antibody 11B8 was developed to detect COR in various host plants infected by P. syringae. The estimated detection limit for COR was 50pg per well, and COR could be reliably quantified from 5 to 40ngml−1. The subcellular localization of COR within infected tomato tissue was investigated using the COR-specific antibody MAb 8H3G2. Immunofluorescence microscopy and immunogold labelling showed that COR was present inside tomato cells and was associated with chloroplasts and particles of proteinase inhibitor I. Localization studies indicated that COR is mobile in infected plant tissue and can be detected in healthy tissue adjacent to the bacterial lesions.</description><subject>Bacterial plant pathogens</subject><subject>Biological and medical sciences</subject><subject>Brassica</subject><subject>cauliflower</subject><subject>collard</subject><subject>coronatine</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Generalities. Techniques. Transmission, epidemiology, ecology. Antibacterial substances, control</subject><subject>Glycinemax</subject><subject>Lycopersicon esculentum</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Pseudomonas syringae</subject><subject>soybean</subject><subject>tomato</subject><issn>0885-5765</issn><issn>1096-1178</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><recordid>eNp1kL1PwzAQxS0EEuVjZfYCW4odO449Ij4rVWIABibLsS-qURoH20WUv56EIjEhnXTLe-_u_RA6o2ROCRGXw3oY5iUhdE4Y43toRokSBaW13EczImVVVLWoDtFRSm-EEMUpnaHXG8hgsw89Di3OK8DDaptDDp--xzbE0Jvse8DNFt8uF09X2PQOd8Gazn-ZH5ufph0zwOGhM33G2ae0gRN00JouwenvPkYvd7fP1w_F8vF-cX21LCzjdS6MYlXjWoBSMMelYgSqWomKcRBEAK-MsVI2tFalJJIaV4kGHBWta5RqScmO0cUud4jhfQMp67VPFrrxFQibpEcnpWU9Cec7oY0hpQitHqJfm7jVlOiJoJ4I6omgngiOhvPfZJPGwm00vfXpz8U5U1xNwXKng7Hmh4eok_XQW3A-jly0C_6_E9_AAIUP</recordid><startdate>20010601</startdate><enddate>20010601</enddate><creator>Zhao, Y.F.</creator><creator>Jones, W.T.</creator><creator>Sutherland, P.</creator><creator>Palmer, D.A.</creator><creator>Mitchell, R.E.</creator><creator>Reynolds, P.H.S.</creator><creator>Damicone, J.P.</creator><creator>Bender, C.L.</creator><general>Elsevier India Pvt Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20010601</creationdate><title>Detection of the phytotoxin coronatine by ELISA and localization in infected plant tissue</title><author>Zhao, Y.F. ; Jones, W.T. ; Sutherland, P. ; Palmer, D.A. ; Mitchell, R.E. ; Reynolds, P.H.S. ; Damicone, J.P. ; Bender, C.L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c347t-a935bdfee263d48930e5796534e606e45aac88b17928081ad56bed16fdb99f023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Bacterial plant pathogens</topic><topic>Biological and medical sciences</topic><topic>Brassica</topic><topic>cauliflower</topic><topic>collard</topic><topic>coronatine</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Generalities. Techniques. Transmission, epidemiology, ecology. Antibacterial substances, control</topic><topic>Glycinemax</topic><topic>Lycopersicon esculentum</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Pseudomonas syringae</topic><topic>soybean</topic><topic>tomato</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Y.F.</creatorcontrib><creatorcontrib>Jones, W.T.</creatorcontrib><creatorcontrib>Sutherland, P.</creatorcontrib><creatorcontrib>Palmer, D.A.</creatorcontrib><creatorcontrib>Mitchell, R.E.</creatorcontrib><creatorcontrib>Reynolds, P.H.S.</creatorcontrib><creatorcontrib>Damicone, J.P.</creatorcontrib><creatorcontrib>Bender, C.L.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Physiological and molecular plant pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Y.F.</au><au>Jones, W.T.</au><au>Sutherland, P.</au><au>Palmer, D.A.</au><au>Mitchell, R.E.</au><au>Reynolds, P.H.S.</au><au>Damicone, J.P.</au><au>Bender, C.L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of the phytotoxin coronatine by ELISA and localization in infected plant tissue</atitle><jtitle>Physiological and molecular plant pathology</jtitle><date>2001-06-01</date><risdate>2001</risdate><volume>58</volume><issue>6</issue><spage>247</spage><epage>258</epage><pages>247-258</pages><issn>0885-5765</issn><eissn>1096-1178</eissn><coden>PMPPEZ</coden><abstract>Several pathovars of Pseudomonas syringae produce the phytotoxin coronatine (COR)in vitro, and the availability of purified toxin has facilitated development of immunological detection methods. A modified, indirect competitive ELISA using the COR-specific monoclonal antibody 11B8 was developed to detect COR in various host plants infected by P. syringae. The estimated detection limit for COR was 50pg per well, and COR could be reliably quantified from 5 to 40ngml−1. The subcellular localization of COR within infected tomato tissue was investigated using the COR-specific antibody MAb 8H3G2. Immunofluorescence microscopy and immunogold labelling showed that COR was present inside tomato cells and was associated with chloroplasts and particles of proteinase inhibitor I. Localization studies indicated that COR is mobile in infected plant tissue and can be detected in healthy tissue adjacent to the bacterial lesions.</abstract><cop>London</cop><pub>Elsevier India Pvt Ltd</pub><doi>10.1006/pmpp.2001.0334</doi><tpages>12</tpages></addata></record> |
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| subjects | Bacterial plant pathogens Biological and medical sciences Brassica cauliflower collard coronatine Fundamental and applied biological sciences. Psychology Generalities. Techniques. Transmission, epidemiology, ecology. Antibacterial substances, control Glycinemax Lycopersicon esculentum Phytopathology. Animal pests. Plant and forest protection Pseudomonas syringae soybean tomato |
| title | Detection of the phytotoxin coronatine by ELISA and localization in infected plant tissue |
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