Characterization of Cu(II) and Cd(II) resistance mechanisms in Sphingobium sp. PHE-SPH and Ochrobactrum sp. PHE-OCH and their potential application in the bioremediation of heavy metal-phenanthrene co-contaminated sites
Soil that is co-contaminated with heavy metals (HMs) and polycyclic aromatic hydrocarbons (PAHs) is difficult to bioremediate due to the ability of toxic metals to inhibit PAH degradation by bacteria. We demonstrated the resistance mechanisms to Cu(II) and Cd(II) of two newly isolated strains of Sph...
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| Veröffentlicht in: | Environmental science and pollution research international 2016-04, Vol.23 (7), p.6861-6872 |
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| creator | Chen, Chen Lei, Wenrui Lu, Min Zhang, Jianan Zhang, Zhou Luo, Chunling Chen, Yahua Hong, Qing Shen, Zhenguo |
| description | Soil that is co-contaminated with heavy metals (HMs) and polycyclic aromatic hydrocarbons (PAHs) is difficult to bioremediate due to the ability of toxic metals to inhibit PAH degradation by bacteria. We demonstrated the resistance mechanisms to Cu(II) and Cd(II) of two newly isolated strains of Sphingobium sp. PHE-SPH and Ochrobactrum sp. PHE-OCH and further tested their potential application in the bioremediation of HM-phenanthrene (PhA) co-contaminated sites. The PHE-SPH and PHE-OCH strains tolerated 4.63 and 4.34 mM Cu(II) and also showed tolerance to 0.48 and 1.52 mM Cd(II), respectively. Diverse resistance patterns were detected between the two strains. In PHE-OCH cells, the maximum accumulation of Cu(II) occurred in the cell wall, while the maximum accumulation was in the cytoplasm of PHE-SPH cells. This resulted in a sudden suppression of growth in PHE-OCH and a gradual inhibition in PHE-SPH as the concentration of Cu(II) increased. Organic acid production was markedly higher in PHE-OCH than in PHE-SPH, which may also have a role in the resistance mechanisms, and contributes to the higher Cd(II) tolerance of PHE-OCH. The factors involved in the absorption of Cu(II) or Cd(II) in PHE-SPH and PHE-OCH were identified as proteins and carbohydrates by Fourier transform infrared (FT-IR) spectroscopy. Furthermore, both strains showed the ability to efficiently degrade PhA and maintained this high degradation efficiency under HM stress. The high tolerance to HMs and the PhA degradation capacity make Sphingobium sp. PHE-SPH and Ochrobactrum sp. PHE-OCH excellent candidate organisms for the bioremediation of HM-PhA co-contaminated sites. |
| doi_str_mv | 10.1007/s11356-015-5926-0 |
| format | Article |
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PHE-SPH and Ochrobactrum sp. PHE-OCH and their potential application in the bioremediation of heavy metal-phenanthrene co-contaminated sites</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Chen, Chen ; Lei, Wenrui ; Lu, Min ; Zhang, Jianan ; Zhang, Zhou ; Luo, Chunling ; Chen, Yahua ; Hong, Qing ; Shen, Zhenguo</creator><creatorcontrib>Chen, Chen ; Lei, Wenrui ; Lu, Min ; Zhang, Jianan ; Zhang, Zhou ; Luo, Chunling ; Chen, Yahua ; Hong, Qing ; Shen, Zhenguo</creatorcontrib><description>Soil that is co-contaminated with heavy metals (HMs) and polycyclic aromatic hydrocarbons (PAHs) is difficult to bioremediate due to the ability of toxic metals to inhibit PAH degradation by bacteria. We demonstrated the resistance mechanisms to Cu(II) and Cd(II) of two newly isolated strains of Sphingobium sp. PHE-SPH and Ochrobactrum sp. PHE-OCH and further tested their potential application in the bioremediation of HM-phenanthrene (PhA) co-contaminated sites. The PHE-SPH and PHE-OCH strains tolerated 4.63 and 4.34 mM Cu(II) and also showed tolerance to 0.48 and 1.52 mM Cd(II), respectively. Diverse resistance patterns were detected between the two strains. In PHE-OCH cells, the maximum accumulation of Cu(II) occurred in the cell wall, while the maximum accumulation was in the cytoplasm of PHE-SPH cells. This resulted in a sudden suppression of growth in PHE-OCH and a gradual inhibition in PHE-SPH as the concentration of Cu(II) increased. Organic acid production was markedly higher in PHE-OCH than in PHE-SPH, which may also have a role in the resistance mechanisms, and contributes to the higher Cd(II) tolerance of PHE-OCH. The factors involved in the absorption of Cu(II) or Cd(II) in PHE-SPH and PHE-OCH were identified as proteins and carbohydrates by Fourier transform infrared (FT-IR) spectroscopy. Furthermore, both strains showed the ability to efficiently degrade PhA and maintained this high degradation efficiency under HM stress. The high tolerance to HMs and the PhA degradation capacity make Sphingobium sp. PHE-SPH and Ochrobactrum sp. PHE-OCH excellent candidate organisms for the bioremediation of HM-PhA co-contaminated sites.</description><identifier>ISSN: 0944-1344</identifier><identifier>EISSN: 1614-7499</identifier><identifier>DOI: 10.1007/s11356-015-5926-0</identifier><identifier>PMID: 26670028</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>absorption ; Acid production ; Anti-Bacterial Agents - pharmacology ; Aquatic Pollution ; Atmospheric Protection/Air Quality Control/Air Pollution ; bacteria ; Biodegradation, Environmental ; Bioremediation ; Cadmium ; Cadmium - analysis ; Cadmium - chemistry ; Cadmium - pharmacology ; Carbohydrates ; cell walls ; Copper ; Copper - analysis ; Copper - chemistry ; Copper - pharmacology ; cytoplasm ; Drug Resistance, Bacterial ; Earth and Environmental Science ; Ecotoxicology ; Environment ; Environmental Chemistry ; Environmental Health ; Environmental science ; Experiments ; Fourier transform infrared spectroscopy ; Fourier transforms ; Geochemistry ; Heavy metals ; Lethal Dose 50 ; Metals ; Microbial Sensitivity Tests ; Microorganisms ; Ochrobactrum ; Ochrobactrum - drug effects ; Ochrobactrum - metabolism ; Organic acids ; Phenanthrene ; Phenanthrenes - analysis ; Phenanthrenes - chemistry ; Phenanthrenes - pharmacology ; Polycyclic aromatic hydrocarbons ; proteins ; Research Article ; resistance mechanisms ; soil ; Soil - chemistry ; Soil contamination ; Soil Microbiology ; Soil Pollutants - analysis ; Soil Pollutants - chemistry ; Soil Pollutants - pharmacology ; Sphingobacterium - drug effects ; Sphingobacterium - metabolism ; Sphingobium ; Sphingomonas ; Studies ; toxicity ; Waste Water Technology ; Water Management ; Water Pollution Control</subject><ispartof>Environmental science and pollution research international, 2016-04, Vol.23 (7), p.6861-6872</ispartof><rights>Springer-Verlag Berlin Heidelberg 2015</rights><rights>Springer-Verlag Berlin Heidelberg 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c466t-ee03721def6e9a701606595123a1cb68cbc4ed4e649e83799441748d726cb643</citedby><cites>FETCH-LOGICAL-c466t-ee03721def6e9a701606595123a1cb68cbc4ed4e649e83799441748d726cb643</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11356-015-5926-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11356-015-5926-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26670028$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Chen</creatorcontrib><creatorcontrib>Lei, Wenrui</creatorcontrib><creatorcontrib>Lu, Min</creatorcontrib><creatorcontrib>Zhang, Jianan</creatorcontrib><creatorcontrib>Zhang, Zhou</creatorcontrib><creatorcontrib>Luo, Chunling</creatorcontrib><creatorcontrib>Chen, Yahua</creatorcontrib><creatorcontrib>Hong, Qing</creatorcontrib><creatorcontrib>Shen, Zhenguo</creatorcontrib><title>Characterization of Cu(II) and Cd(II) resistance mechanisms in Sphingobium sp. PHE-SPH and Ochrobactrum sp. PHE-OCH and their potential application in the bioremediation of heavy metal-phenanthrene co-contaminated sites</title><title>Environmental science and pollution research international</title><addtitle>Environ Sci Pollut Res</addtitle><addtitle>Environ Sci Pollut Res Int</addtitle><description>Soil that is co-contaminated with heavy metals (HMs) and polycyclic aromatic hydrocarbons (PAHs) is difficult to bioremediate due to the ability of toxic metals to inhibit PAH degradation by bacteria. We demonstrated the resistance mechanisms to Cu(II) and Cd(II) of two newly isolated strains of Sphingobium sp. PHE-SPH and Ochrobactrum sp. PHE-OCH and further tested their potential application in the bioremediation of HM-phenanthrene (PhA) co-contaminated sites. The PHE-SPH and PHE-OCH strains tolerated 4.63 and 4.34 mM Cu(II) and also showed tolerance to 0.48 and 1.52 mM Cd(II), respectively. Diverse resistance patterns were detected between the two strains. In PHE-OCH cells, the maximum accumulation of Cu(II) occurred in the cell wall, while the maximum accumulation was in the cytoplasm of PHE-SPH cells. This resulted in a sudden suppression of growth in PHE-OCH and a gradual inhibition in PHE-SPH as the concentration of Cu(II) increased. Organic acid production was markedly higher in PHE-OCH than in PHE-SPH, which may also have a role in the resistance mechanisms, and contributes to the higher Cd(II) tolerance of PHE-OCH. The factors involved in the absorption of Cu(II) or Cd(II) in PHE-SPH and PHE-OCH were identified as proteins and carbohydrates by Fourier transform infrared (FT-IR) spectroscopy. Furthermore, both strains showed the ability to efficiently degrade PhA and maintained this high degradation efficiency under HM stress. The high tolerance to HMs and the PhA degradation capacity make Sphingobium sp. PHE-SPH and Ochrobactrum sp. PHE-OCH excellent candidate organisms for the bioremediation of HM-PhA co-contaminated sites.</description><subject>absorption</subject><subject>Acid production</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Aquatic Pollution</subject><subject>Atmospheric Protection/Air Quality Control/Air Pollution</subject><subject>bacteria</subject><subject>Biodegradation, Environmental</subject><subject>Bioremediation</subject><subject>Cadmium</subject><subject>Cadmium - analysis</subject><subject>Cadmium - chemistry</subject><subject>Cadmium - pharmacology</subject><subject>Carbohydrates</subject><subject>cell walls</subject><subject>Copper</subject><subject>Copper - analysis</subject><subject>Copper - chemistry</subject><subject>Copper - pharmacology</subject><subject>cytoplasm</subject><subject>Drug Resistance, Bacterial</subject><subject>Earth and Environmental Science</subject><subject>Ecotoxicology</subject><subject>Environment</subject><subject>Environmental Chemistry</subject><subject>Environmental Health</subject><subject>Environmental science</subject><subject>Experiments</subject><subject>Fourier transform infrared spectroscopy</subject><subject>Fourier transforms</subject><subject>Geochemistry</subject><subject>Heavy metals</subject><subject>Lethal Dose 50</subject><subject>Metals</subject><subject>Microbial Sensitivity Tests</subject><subject>Microorganisms</subject><subject>Ochrobactrum</subject><subject>Ochrobactrum - drug effects</subject><subject>Ochrobactrum - metabolism</subject><subject>Organic acids</subject><subject>Phenanthrene</subject><subject>Phenanthrenes - analysis</subject><subject>Phenanthrenes - chemistry</subject><subject>Phenanthrenes - pharmacology</subject><subject>Polycyclic aromatic hydrocarbons</subject><subject>proteins</subject><subject>Research Article</subject><subject>resistance mechanisms</subject><subject>soil</subject><subject>Soil - chemistry</subject><subject>Soil contamination</subject><subject>Soil Microbiology</subject><subject>Soil Pollutants - analysis</subject><subject>Soil Pollutants - chemistry</subject><subject>Soil Pollutants - pharmacology</subject><subject>Sphingobacterium - drug effects</subject><subject>Sphingobacterium - metabolism</subject><subject>Sphingobium</subject><subject>Sphingomonas</subject><subject>Studies</subject><subject>toxicity</subject><subject>Waste Water Technology</subject><subject>Water Management</subject><subject>Water Pollution Control</subject><issn>0944-1344</issn><issn>1614-7499</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNksFu1DAQhiMEokvhAbiAJS7lkGI7Xjs-oqiwK1VqpS1ny3FmN64SO9gOUnlVXgZvU6qKA-Lksf5vfo9Hf1G8JficYCw-RUKqNS8xWZdrSXPxrFgRTlgpmJTPixWWjJWkYuykeBXjLcYUSypeFieUc5Fv9ar41fQ6aJMg2J86We-Q36NmPttuPyLtOtR092WAaGPSzgAawfTa2ThGZB3aTb11B9_aeURxOkfXm4tyd725770yffBtNg9PxKtmEVMPNqDJJ3DJ6gHpaRqsWUbIvllGrfUBRujs42A96B93eYKkh3LqwWmX-gAOkPGl8S7p0TqdoEPRJoivixd7PUR483CeFjdfLm6aTXl59XXbfL4sDeM8lQC4EpR0sOcgtcCEY76Wa0IrTUzLa9MaBh0DziTUlZB5qUSwuhOUZ5lVp8XZYjsF_32GmNRoo4Fh0A78HBUREkuBa0r-AxX1kRQyox_-Qm_9HFz-R6bqmmNWsSNFFsoEH2OAvZqCHXW4UwSrY0bUkhGVM6KOGVE497x7cJ7bvN3Hjj-hyABdgJgld4Dw5Ol_uL5fmvbaK30INqpvO3pcJiYVZZJUvwH_5dDK</recordid><startdate>20160401</startdate><enddate>20160401</enddate><creator>Chen, Chen</creator><creator>Lei, Wenrui</creator><creator>Lu, Min</creator><creator>Zhang, Jianan</creator><creator>Zhang, Zhou</creator><creator>Luo, Chunling</creator><creator>Chen, Yahua</creator><creator>Hong, Qing</creator><creator>Shen, Zhenguo</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7SN</scope><scope>7T7</scope><scope>7TV</scope><scope>7U7</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>P64</scope><scope>PATMY</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>7X8</scope><scope>7ST</scope><scope>SOI</scope></search><sort><creationdate>20160401</creationdate><title>Characterization of Cu(II) and Cd(II) resistance mechanisms in Sphingobium sp. PHE-SPH and Ochrobactrum sp. PHE-OCH and their potential application in the bioremediation of heavy metal-phenanthrene co-contaminated sites</title><author>Chen, Chen ; Lei, Wenrui ; Lu, Min ; Zhang, Jianan ; Zhang, Zhou ; Luo, Chunling ; Chen, Yahua ; Hong, Qing ; Shen, Zhenguo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c466t-ee03721def6e9a701606595123a1cb68cbc4ed4e649e83799441748d726cb643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>absorption</topic><topic>Acid production</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Aquatic Pollution</topic><topic>Atmospheric Protection/Air Quality Control/Air Pollution</topic><topic>bacteria</topic><topic>Biodegradation, Environmental</topic><topic>Bioremediation</topic><topic>Cadmium</topic><topic>Cadmium - analysis</topic><topic>Cadmium - chemistry</topic><topic>Cadmium - pharmacology</topic><topic>Carbohydrates</topic><topic>cell walls</topic><topic>Copper</topic><topic>Copper - analysis</topic><topic>Copper - chemistry</topic><topic>Copper - pharmacology</topic><topic>cytoplasm</topic><topic>Drug Resistance, Bacterial</topic><topic>Earth and Environmental Science</topic><topic>Ecotoxicology</topic><topic>Environment</topic><topic>Environmental Chemistry</topic><topic>Environmental Health</topic><topic>Environmental science</topic><topic>Experiments</topic><topic>Fourier transform infrared spectroscopy</topic><topic>Fourier transforms</topic><topic>Geochemistry</topic><topic>Heavy metals</topic><topic>Lethal Dose 50</topic><topic>Metals</topic><topic>Microbial Sensitivity Tests</topic><topic>Microorganisms</topic><topic>Ochrobactrum</topic><topic>Ochrobactrum - drug effects</topic><topic>Ochrobactrum - metabolism</topic><topic>Organic acids</topic><topic>Phenanthrene</topic><topic>Phenanthrenes - analysis</topic><topic>Phenanthrenes - chemistry</topic><topic>Phenanthrenes - pharmacology</topic><topic>Polycyclic aromatic hydrocarbons</topic><topic>proteins</topic><topic>Research Article</topic><topic>resistance mechanisms</topic><topic>soil</topic><topic>Soil - chemistry</topic><topic>Soil contamination</topic><topic>Soil Microbiology</topic><topic>Soil Pollutants - analysis</topic><topic>Soil Pollutants - chemistry</topic><topic>Soil Pollutants - pharmacology</topic><topic>Sphingobacterium - drug effects</topic><topic>Sphingobacterium - metabolism</topic><topic>Sphingobium</topic><topic>Sphingomonas</topic><topic>Studies</topic><topic>toxicity</topic><topic>Waste Water Technology</topic><topic>Water Management</topic><topic>Water Pollution Control</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Chen</creatorcontrib><creatorcontrib>Lei, Wenrui</creatorcontrib><creatorcontrib>Lu, Min</creatorcontrib><creatorcontrib>Zhang, Jianan</creatorcontrib><creatorcontrib>Zhang, Zhou</creatorcontrib><creatorcontrib>Luo, Chunling</creatorcontrib><creatorcontrib>Chen, Yahua</creatorcontrib><creatorcontrib>Hong, Qing</creatorcontrib><creatorcontrib>Shen, Zhenguo</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ecology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Pollution Abstracts</collection><collection>Toxicology Abstracts</collection><collection>ABI/INFORM Collection</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Global (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Business Premium Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Business Premium Collection (Alumni)</collection><collection>Health Research Premium Collection</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ABI/INFORM Professional Advanced</collection><collection>ABI/INFORM Global</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>ProQuest One Business</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>Environment Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Environmental science and pollution research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Chen</au><au>Lei, Wenrui</au><au>Lu, Min</au><au>Zhang, Jianan</au><au>Zhang, Zhou</au><au>Luo, Chunling</au><au>Chen, Yahua</au><au>Hong, Qing</au><au>Shen, Zhenguo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of Cu(II) and Cd(II) resistance mechanisms in Sphingobium sp. PHE-SPH and Ochrobactrum sp. PHE-OCH and their potential application in the bioremediation of heavy metal-phenanthrene co-contaminated sites</atitle><jtitle>Environmental science and pollution research international</jtitle><stitle>Environ Sci Pollut Res</stitle><addtitle>Environ Sci Pollut Res Int</addtitle><date>2016-04-01</date><risdate>2016</risdate><volume>23</volume><issue>7</issue><spage>6861</spage><epage>6872</epage><pages>6861-6872</pages><issn>0944-1344</issn><eissn>1614-7499</eissn><abstract>Soil that is co-contaminated with heavy metals (HMs) and polycyclic aromatic hydrocarbons (PAHs) is difficult to bioremediate due to the ability of toxic metals to inhibit PAH degradation by bacteria. We demonstrated the resistance mechanisms to Cu(II) and Cd(II) of two newly isolated strains of Sphingobium sp. PHE-SPH and Ochrobactrum sp. PHE-OCH and further tested their potential application in the bioremediation of HM-phenanthrene (PhA) co-contaminated sites. The PHE-SPH and PHE-OCH strains tolerated 4.63 and 4.34 mM Cu(II) and also showed tolerance to 0.48 and 1.52 mM Cd(II), respectively. Diverse resistance patterns were detected between the two strains. In PHE-OCH cells, the maximum accumulation of Cu(II) occurred in the cell wall, while the maximum accumulation was in the cytoplasm of PHE-SPH cells. This resulted in a sudden suppression of growth in PHE-OCH and a gradual inhibition in PHE-SPH as the concentration of Cu(II) increased. Organic acid production was markedly higher in PHE-OCH than in PHE-SPH, which may also have a role in the resistance mechanisms, and contributes to the higher Cd(II) tolerance of PHE-OCH. The factors involved in the absorption of Cu(II) or Cd(II) in PHE-SPH and PHE-OCH were identified as proteins and carbohydrates by Fourier transform infrared (FT-IR) spectroscopy. Furthermore, both strains showed the ability to efficiently degrade PhA and maintained this high degradation efficiency under HM stress. The high tolerance to HMs and the PhA degradation capacity make Sphingobium sp. PHE-SPH and Ochrobactrum sp. PHE-OCH excellent candidate organisms for the bioremediation of HM-PhA co-contaminated sites.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>26670028</pmid><doi>10.1007/s11356-015-5926-0</doi><tpages>12</tpages></addata></record> |
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| ispartof | Environmental science and pollution research international, 2016-04, Vol.23 (7), p.6861-6872 |
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| recordid | cdi_proquest_miscellaneous_1790970821 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | absorption Acid production Anti-Bacterial Agents - pharmacology Aquatic Pollution Atmospheric Protection/Air Quality Control/Air Pollution bacteria Biodegradation, Environmental Bioremediation Cadmium Cadmium - analysis Cadmium - chemistry Cadmium - pharmacology Carbohydrates cell walls Copper Copper - analysis Copper - chemistry Copper - pharmacology cytoplasm Drug Resistance, Bacterial Earth and Environmental Science Ecotoxicology Environment Environmental Chemistry Environmental Health Environmental science Experiments Fourier transform infrared spectroscopy Fourier transforms Geochemistry Heavy metals Lethal Dose 50 Metals Microbial Sensitivity Tests Microorganisms Ochrobactrum Ochrobactrum - drug effects Ochrobactrum - metabolism Organic acids Phenanthrene Phenanthrenes - analysis Phenanthrenes - chemistry Phenanthrenes - pharmacology Polycyclic aromatic hydrocarbons proteins Research Article resistance mechanisms soil Soil - chemistry Soil contamination Soil Microbiology Soil Pollutants - analysis Soil Pollutants - chemistry Soil Pollutants - pharmacology Sphingobacterium - drug effects Sphingobacterium - metabolism Sphingobium Sphingomonas Studies toxicity Waste Water Technology Water Management Water Pollution Control |
| title | Characterization of Cu(II) and Cd(II) resistance mechanisms in Sphingobium sp. PHE-SPH and Ochrobactrum sp. PHE-OCH and their potential application in the bioremediation of heavy metal-phenanthrene co-contaminated sites |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T10%3A27%3A40IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Characterization%20of%20Cu(II)%20and%20Cd(II)%20resistance%20mechanisms%20in%20Sphingobium%20sp.%20PHE-SPH%20and%20Ochrobactrum%20sp.%20PHE-OCH%20and%20their%20potential%20application%20in%20the%20bioremediation%20of%20heavy%20metal-phenanthrene%20co-contaminated%20sites&rft.jtitle=Environmental%20science%20and%20pollution%20research%20international&rft.au=Chen,%20Chen&rft.date=2016-04-01&rft.volume=23&rft.issue=7&rft.spage=6861&rft.epage=6872&rft.pages=6861-6872&rft.issn=0944-1344&rft.eissn=1614-7499&rft_id=info:doi/10.1007/s11356-015-5926-0&rft_dat=%3Cproquest_cross%3E1790970821%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1788604349&rft_id=info:pmid/26670028&rfr_iscdi=true |