Comparing the ability of luminex xMAP super( registered ) salmonella serotyping assay and traditional serotyping method for serotyping salmonella isolated from southern Chinese population

Comparing the ability of luminex xMAP super( registered ) salmonella serotyping assay and traditional serotyping method for serotyping salmonella isolated from southern Chinese population

Aims To investigate whether the Luminex xMAP super( registered ) Salmonella Serotyping Assay (xMAP SSA) is applicable to serotype Salmonella from humans in southern China. Methods and Results Two hundred and five Salmonella isolates from diarrhoea patients were serotyped by xMAP SSA in parallel with...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of applied microbiology 2016-06, Vol.120 (6), p.1668-1676
Hauptverfasser: Liang, D W, Lu, J H, Wu, Q, Ke, B X, Jiang, CH, Long, J, Fang, Y P, Lin, L J, Zeng, N Y, Fu, L, Jiang, L X
Format: Artikel
Sprache:eng
Schlagworte:
Salmonella enteritidis
Salmonella typhimurium
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 1676
container_issue 6
container_start_page 1668
container_title Journal of applied microbiology
container_volume 120
creator Liang, D W
Lu, J H
Wu, Q
Ke, B X
Jiang, CH
Long, J
Fang, Y P
Lin, L J
Zeng, N Y
Fu, L
Jiang, L X
description Aims To investigate whether the Luminex xMAP super( registered ) Salmonella Serotyping Assay (xMAP SSA) is applicable to serotype Salmonella from humans in southern China. Methods and Results Two hundred and five Salmonella isolates from diarrhoea patients were serotyped by xMAP SSA in parallel with the traditional serotyping. Forty serotypes were identified among 205 isolates; the most prevalent serotypes identified were Salmonella Enteritidis, Salmonella Stanley, Salmonella I 4,5,12:i:-, and Salmonella Typhimurium. One hundred and ninety-five (95.1%, 195/205) isolates were serotyped completely by xMAP SSA, while 10 stereotypes were partially detected as they were not included in the assay. The xMAP SSA correctly identified 192 (98.5%, 192/195) isolates. Five nonmotile and three monophasic strains, which possessed flagellar antigen genes that weren't expressed, were completely serotyped by xMAP SSA; however, these isolates were left undetected by the traditional method. Conclusion The xMAP SSA used in the study is a microsphere-based, molecular assay that could rapidly and accurately serotype Salmonella. It is suitable to identify the serovars of Salmonella in southern China. Significance and Impact of the Study The xMAP SSA, with high-throughput characteristics, provides an accurate and rapid serotyping system that dramatically strengthens the capability of clinical and public health laboratories for Salmonella serotyping.
doi_str_mv 10.1111/jam.13106
format Article
fullrecord <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_miscellaneous_1790967416</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1790967416</sourcerecordid><originalsourceid>FETCH-proquest_miscellaneous_17909674163</originalsourceid><addsrcrecordid>eNqVjj9PwzAQxS0EEuXPwDe4sQwpdtIk6ogiEAsSA3tlyKVxZfuMz5Gaz8aXw0UMXbnlne6efu8JcafkSuV52Gu3UpWSzZlYqKqpi7Jpy_PffV3Usi0vxRXzXkpVybpZiO-OXNDR-B2kEUF_GGvSDDSAnZzxeIDD6-Mb8BQwLiHiznDCiD3cA2vryKO1GhgjpTkcKZpZz6B9Dynq3iRDXttTg8M0Ug8DxdPrCcwwWZ1yxBDJAdOUi0UP3ZjrMEKgMOV_5t6Ii0Fbxts_vRbL56f37qUIkb4m5LR1hj-PTI808Va1G7lp2rVqqn9YfwArgXIB</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1790967416</pqid></control><display><type>article</type><title>Comparing the ability of luminex xMAP super( registered ) salmonella serotyping assay and traditional serotyping method for serotyping salmonella isolated from southern Chinese population</title><source>Wiley Journals</source><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>Liang, D W ; Lu, J H ; Wu, Q ; Ke, B X ; Jiang, CH ; Long, J ; Fang, Y P ; Lin, L J ; Zeng, N Y ; Fu, L ; Jiang, L X</creator><creatorcontrib>Liang, D W ; Lu, J H ; Wu, Q ; Ke, B X ; Jiang, CH ; Long, J ; Fang, Y P ; Lin, L J ; Zeng, N Y ; Fu, L ; Jiang, L X</creatorcontrib><description>Aims To investigate whether the Luminex xMAP super( registered ) Salmonella Serotyping Assay (xMAP SSA) is applicable to serotype Salmonella from humans in southern China. Methods and Results Two hundred and five Salmonella isolates from diarrhoea patients were serotyped by xMAP SSA in parallel with the traditional serotyping. Forty serotypes were identified among 205 isolates; the most prevalent serotypes identified were Salmonella Enteritidis, Salmonella Stanley, Salmonella I 4,5,12:i:-, and Salmonella Typhimurium. One hundred and ninety-five (95.1%, 195/205) isolates were serotyped completely by xMAP SSA, while 10 stereotypes were partially detected as they were not included in the assay. The xMAP SSA correctly identified 192 (98.5%, 192/195) isolates. Five nonmotile and three monophasic strains, which possessed flagellar antigen genes that weren't expressed, were completely serotyped by xMAP SSA; however, these isolates were left undetected by the traditional method. Conclusion The xMAP SSA used in the study is a microsphere-based, molecular assay that could rapidly and accurately serotype Salmonella. It is suitable to identify the serovars of Salmonella in southern China. Significance and Impact of the Study The xMAP SSA, with high-throughput characteristics, provides an accurate and rapid serotyping system that dramatically strengthens the capability of clinical and public health laboratories for Salmonella serotyping.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.13106</identifier><language>eng</language><subject>Salmonella enteritidis ; Salmonella typhimurium</subject><ispartof>Journal of applied microbiology, 2016-06, Vol.120 (6), p.1668-1676</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Liang, D W</creatorcontrib><creatorcontrib>Lu, J H</creatorcontrib><creatorcontrib>Wu, Q</creatorcontrib><creatorcontrib>Ke, B X</creatorcontrib><creatorcontrib>Jiang, CH</creatorcontrib><creatorcontrib>Long, J</creatorcontrib><creatorcontrib>Fang, Y P</creatorcontrib><creatorcontrib>Lin, L J</creatorcontrib><creatorcontrib>Zeng, N Y</creatorcontrib><creatorcontrib>Fu, L</creatorcontrib><creatorcontrib>Jiang, L X</creatorcontrib><title>Comparing the ability of luminex xMAP super( registered ) salmonella serotyping assay and traditional serotyping method for serotyping salmonella isolated from southern Chinese population</title><title>Journal of applied microbiology</title><description>Aims To investigate whether the Luminex xMAP super( registered ) Salmonella Serotyping Assay (xMAP SSA) is applicable to serotype Salmonella from humans in southern China. Methods and Results Two hundred and five Salmonella isolates from diarrhoea patients were serotyped by xMAP SSA in parallel with the traditional serotyping. Forty serotypes were identified among 205 isolates; the most prevalent serotypes identified were Salmonella Enteritidis, Salmonella Stanley, Salmonella I 4,5,12:i:-, and Salmonella Typhimurium. One hundred and ninety-five (95.1%, 195/205) isolates were serotyped completely by xMAP SSA, while 10 stereotypes were partially detected as they were not included in the assay. The xMAP SSA correctly identified 192 (98.5%, 192/195) isolates. Five nonmotile and three monophasic strains, which possessed flagellar antigen genes that weren't expressed, were completely serotyped by xMAP SSA; however, these isolates were left undetected by the traditional method. Conclusion The xMAP SSA used in the study is a microsphere-based, molecular assay that could rapidly and accurately serotype Salmonella. It is suitable to identify the serovars of Salmonella in southern China. Significance and Impact of the Study The xMAP SSA, with high-throughput characteristics, provides an accurate and rapid serotyping system that dramatically strengthens the capability of clinical and public health laboratories for Salmonella serotyping.</description><subject>Salmonella enteritidis</subject><subject>Salmonella typhimurium</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNqVjj9PwzAQxS0EEuXPwDe4sQwpdtIk6ogiEAsSA3tlyKVxZfuMz5Gaz8aXw0UMXbnlne6efu8JcafkSuV52Gu3UpWSzZlYqKqpi7Jpy_PffV3Usi0vxRXzXkpVybpZiO-OXNDR-B2kEUF_GGvSDDSAnZzxeIDD6-Mb8BQwLiHiznDCiD3cA2vryKO1GhgjpTkcKZpZz6B9Dynq3iRDXttTg8M0Ug8DxdPrCcwwWZ1yxBDJAdOUi0UP3ZjrMEKgMOV_5t6Ii0Fbxts_vRbL56f37qUIkb4m5LR1hj-PTI808Va1G7lp2rVqqn9YfwArgXIB</recordid><startdate>20160601</startdate><enddate>20160601</enddate><creator>Liang, D W</creator><creator>Lu, J H</creator><creator>Wu, Q</creator><creator>Ke, B X</creator><creator>Jiang, CH</creator><creator>Long, J</creator><creator>Fang, Y P</creator><creator>Lin, L J</creator><creator>Zeng, N Y</creator><creator>Fu, L</creator><creator>Jiang, L X</creator><scope>7QL</scope><scope>7ST</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>SOI</scope></search><sort><creationdate>20160601</creationdate><title>Comparing the ability of luminex xMAP super( registered ) salmonella serotyping assay and traditional serotyping method for serotyping salmonella isolated from southern Chinese population</title><author>Liang, D W ; Lu, J H ; Wu, Q ; Ke, B X ; Jiang, CH ; Long, J ; Fang, Y P ; Lin, L J ; Zeng, N Y ; Fu, L ; Jiang, L X</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_miscellaneous_17909674163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Salmonella enteritidis</topic><topic>Salmonella typhimurium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liang, D W</creatorcontrib><creatorcontrib>Lu, J H</creatorcontrib><creatorcontrib>Wu, Q</creatorcontrib><creatorcontrib>Ke, B X</creatorcontrib><creatorcontrib>Jiang, CH</creatorcontrib><creatorcontrib>Long, J</creatorcontrib><creatorcontrib>Fang, Y P</creatorcontrib><creatorcontrib>Lin, L J</creatorcontrib><creatorcontrib>Zeng, N Y</creatorcontrib><creatorcontrib>Fu, L</creatorcontrib><creatorcontrib>Jiang, L X</creatorcontrib><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liang, D W</au><au>Lu, J H</au><au>Wu, Q</au><au>Ke, B X</au><au>Jiang, CH</au><au>Long, J</au><au>Fang, Y P</au><au>Lin, L J</au><au>Zeng, N Y</au><au>Fu, L</au><au>Jiang, L X</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparing the ability of luminex xMAP super( registered ) salmonella serotyping assay and traditional serotyping method for serotyping salmonella isolated from southern Chinese population</atitle><jtitle>Journal of applied microbiology</jtitle><date>2016-06-01</date><risdate>2016</risdate><volume>120</volume><issue>6</issue><spage>1668</spage><epage>1676</epage><pages>1668-1676</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>Aims To investigate whether the Luminex xMAP super( registered ) Salmonella Serotyping Assay (xMAP SSA) is applicable to serotype Salmonella from humans in southern China. Methods and Results Two hundred and five Salmonella isolates from diarrhoea patients were serotyped by xMAP SSA in parallel with the traditional serotyping. Forty serotypes were identified among 205 isolates; the most prevalent serotypes identified were Salmonella Enteritidis, Salmonella Stanley, Salmonella I 4,5,12:i:-, and Salmonella Typhimurium. One hundred and ninety-five (95.1%, 195/205) isolates were serotyped completely by xMAP SSA, while 10 stereotypes were partially detected as they were not included in the assay. The xMAP SSA correctly identified 192 (98.5%, 192/195) isolates. Five nonmotile and three monophasic strains, which possessed flagellar antigen genes that weren't expressed, were completely serotyped by xMAP SSA; however, these isolates were left undetected by the traditional method. Conclusion The xMAP SSA used in the study is a microsphere-based, molecular assay that could rapidly and accurately serotype Salmonella. It is suitable to identify the serovars of Salmonella in southern China. Significance and Impact of the Study The xMAP SSA, with high-throughput characteristics, provides an accurate and rapid serotyping system that dramatically strengthens the capability of clinical and public health laboratories for Salmonella serotyping.</abstract><doi>10.1111/jam.13106</doi></addata></record>
fulltext fulltext
identifier ISSN: 1364-5072
ispartof Journal of applied microbiology, 2016-06, Vol.120 (6), p.1668-1676
issn 1364-5072
1365-2672
language eng
recordid cdi_proquest_miscellaneous_1790967416
source Wiley Journals; Oxford University Press Journals All Titles (1996-Current)
subjects Salmonella enteritidis
Salmonella typhimurium
title Comparing the ability of luminex xMAP super( registered ) salmonella serotyping assay and traditional serotyping method for serotyping salmonella isolated from southern Chinese population
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T12%3A20%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparing%20the%20ability%20of%20luminex%20xMAP%20super(%20registered%20)%20salmonella%20serotyping%20assay%20and%20traditional%20serotyping%20method%20for%20serotyping%20salmonella%20isolated%20from%20southern%20Chinese%20population&rft.jtitle=Journal%20of%20applied%20microbiology&rft.au=Liang,%20D%20W&rft.date=2016-06-01&rft.volume=120&rft.issue=6&rft.spage=1668&rft.epage=1676&rft.pages=1668-1676&rft.issn=1364-5072&rft.eissn=1365-2672&rft_id=info:doi/10.1111/jam.13106&rft_dat=%3Cproquest%3E1790967416%3C/proquest%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1790967416&rft_id=info:pmid/&rfr_iscdi=true