Effect of Hypercholesterolemia on Fatty Infiltration and Quality of Tendon-to-Bone Healing in a Rabbit Model of a Chronic Rotator Cuff Tear: Electrophysiological, Biomechanical, and Histological Analyses
Background: The incidence of healing failure after rotator cuff repair is high, and fatty infiltration is a crucial factor in healing failure. Purpose: To verify the effect of hypercholesterolemia on fatty infiltration and the quality of tendon-to-bone healing and its reversibility by lowering the c...
Gespeichert in:
| Veröffentlicht in: | The American journal of sports medicine 2016-05, Vol.44 (5), p.1153-1164 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1164 |
|---|---|
| container_issue | 5 |
| container_start_page | 1153 |
| container_title | The American journal of sports medicine |
| container_volume | 44 |
| creator | Chung, Seok Won Park, HaeBong Kwon, Jieun Choe, Ghee Young Kim, Sae Hoon Oh, Joo Han |
| description | Background:
The incidence of healing failure after rotator cuff repair is high, and fatty infiltration is a crucial factor in healing failure.
Purpose:
To verify the effect of hypercholesterolemia on fatty infiltration and the quality of tendon-to-bone healing and its reversibility by lowering the cholesterol level in a chronic tear model using the rabbit supraspinatus.
Study Design:
Controlled laboratory study.
Methods:
Forty-eight rabbits were randomly allocated into 4 groups (n = 12 each). After 4 weeks of a high-cholesterol diet (groups A and B) and a regular diet (groups C and D), the supraspinatus tendon was detached and left alone for 6 weeks and then was repaired in a transosseous manner (groups A, B, and C). Group D served as a control. Group A continued to receive the high-cholesterol diet until the final evaluation (6 weeks after repair); however, at the time of repair, group B was changed to a general diet with administration of a cholesterol-lowering agent (simvastatin). Histological evaluation of the fat-to-muscle proportion was performed twice, at the time of repair and the final evaluation, and an electromyographic (EMG) test, mechanical test, and histological test of tendon-to-bone healing were performed at the final evaluation.
Results:
For the EMG test, group A showed a significantly smaller area of compound muscle action potential compared with groups C and D (all P |
| doi_str_mv | 10.1177/0363546515627816 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1790964675</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sage_id>10.1177_0363546515627816</sage_id><sourcerecordid>4070802211</sourcerecordid><originalsourceid>FETCH-LOGICAL-c351t-ab73a147d8ab0026031d5c74691c93ed1ef0e214f5280dcaefdd8a392887279c3</originalsourceid><addsrcrecordid>eNqNkU1rFTEYhYNY7G1170oCbtyM5mPyMUu9tN5CpVjqesgkb9opc5Nrklnc3-CfNsNtRQqFrl7Iec4Jh4PQe0o-U6rUF8IlF60UVEimNJWv0IoKwRrOpXiNVovcLPoxOsn5nhBCldRv0DGTHWVM8xX6c-Y92IKjx5v9DpK9ixPkAqme7WhwDPjclLLHF8GPU0mmjPXJBId_zmYaq1CdNxBcDE2JzbcYAG-gKuEWjxXE12YYxoJ_RAfTwhq8vksxjBZfx2JKTHg9-yXCpLfoyJspw7uHe4p-nZ_drDfN5dX3i_XXy8ZyQUtjBsUNbZXTZiCEScKpE1a1tZPtODgKngCjrRdME2cNeFdR3jGtFVOd5afo0yF3l-Lvubbtt2O2ME0mQJxzT1VHOtlKJV6Aas0YZ4RW9OMT9D7OKdQiSyBjlMu2qxQ5UDbFnBP4fpfGrUn7npJ-2bR_umm1fHgInoctuH-GxxEr0ByAbG7hv1-fC_wLAjGnug</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1792213649</pqid></control><display><type>article</type><title>Effect of Hypercholesterolemia on Fatty Infiltration and Quality of Tendon-to-Bone Healing in a Rabbit Model of a Chronic Rotator Cuff Tear: Electrophysiological, Biomechanical, and Histological Analyses</title><source>MEDLINE</source><source>SAGE Complete A-Z List</source><source>Alma/SFX Local Collection</source><creator>Chung, Seok Won ; Park, HaeBong ; Kwon, Jieun ; Choe, Ghee Young ; Kim, Sae Hoon ; Oh, Joo Han</creator><creatorcontrib>Chung, Seok Won ; Park, HaeBong ; Kwon, Jieun ; Choe, Ghee Young ; Kim, Sae Hoon ; Oh, Joo Han</creatorcontrib><description>Background:
The incidence of healing failure after rotator cuff repair is high, and fatty infiltration is a crucial factor in healing failure.
Purpose:
To verify the effect of hypercholesterolemia on fatty infiltration and the quality of tendon-to-bone healing and its reversibility by lowering the cholesterol level in a chronic tear model using the rabbit supraspinatus.
Study Design:
Controlled laboratory study.
Methods:
Forty-eight rabbits were randomly allocated into 4 groups (n = 12 each). After 4 weeks of a high-cholesterol diet (groups A and B) and a regular diet (groups C and D), the supraspinatus tendon was detached and left alone for 6 weeks and then was repaired in a transosseous manner (groups A, B, and C). Group D served as a control. Group A continued to receive the high-cholesterol diet until the final evaluation (6 weeks after repair); however, at the time of repair, group B was changed to a general diet with administration of a cholesterol-lowering agent (simvastatin). Histological evaluation of the fat-to-muscle proportion was performed twice, at the time of repair and the final evaluation, and an electromyographic (EMG) test, mechanical test, and histological test of tendon-to-bone healing were performed at the final evaluation.
Results:
For the EMG test, group A showed a significantly smaller area of compound muscle action potential compared with groups C and D (all P <.01), and group B showed a larger area than group A, almost up to the level of group C (P = .312). Similarly, group A showed significantly lower mechanical properties both in load-to-failure and stiffness compared with groups C and D (all P <.05). In addition, although not significantly different, the mechanical properties of group B were higher than those of group A (mean load-to-failure: group A = 42.01 N, group B = 58.23 N [P = .103]; mean stiffness: group A = 36.32 N/mm, group B = 47.22 N/mm [P = .153]). For the histological test, groups A and B showed a significantly higher fat-to-muscle proportion than did groups C and D at 6 weeks after detachment (all P <.05), but at the final evaluation, group B showed a decreased fat-to-muscle proportion (mean ± SD: from 64.02% ± 11.87% to 54.68% ± 10.47%; P = .146) compared with group A, which showed increased fat-to-muscle proportion (from 59.26% ± 17.80% to 78.23% ± 10.87%; P = .015). Groups B and C showed better tendon-to-bone interface structures than did group A, which showed coarse and poorly organized collagen fibers with fat interposition.
Conclusion:
Hypercholesterolemia had a deleterious effect on fatty infiltration and the quality of tendon-to-bone repair site, and lowering hypercholesterolemia seemed to halt or reverse these harmful effects in this experimental model.
Clinical Relevance:
Systemic diseases such as hypercholesterolemia should be tightly controlled during the perioperative period of rotator cuff repair.</description><identifier>ISSN: 0363-5465</identifier><identifier>EISSN: 1552-3365</identifier><identifier>DOI: 10.1177/0363546515627816</identifier><identifier>PMID: 26912283</identifier><identifier>CODEN: AJSMDO</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Animals ; Biomechanics ; Body fat ; Bone and Bones - physiology ; Diet ; Disease Models, Animal ; Fats - metabolism ; Histology ; Hypercholesterolemia - physiopathology ; Male ; Physiology ; Rabbits ; Random Allocation ; Rotator Cuff Injuries - metabolism ; Rotator Cuff Injuries - surgery ; Tissue engineering ; Wound Healing</subject><ispartof>The American journal of sports medicine, 2016-05, Vol.44 (5), p.1153-1164</ispartof><rights>2016 The Author(s)</rights><rights>2016 The Author(s).</rights><rights>Copyright Sage Publications Ltd. May 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c351t-ab73a147d8ab0026031d5c74691c93ed1ef0e214f5280dcaefdd8a392887279c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/0363546515627816$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/0363546515627816$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,780,784,21819,27924,27925,43621,43622</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26912283$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chung, Seok Won</creatorcontrib><creatorcontrib>Park, HaeBong</creatorcontrib><creatorcontrib>Kwon, Jieun</creatorcontrib><creatorcontrib>Choe, Ghee Young</creatorcontrib><creatorcontrib>Kim, Sae Hoon</creatorcontrib><creatorcontrib>Oh, Joo Han</creatorcontrib><title>Effect of Hypercholesterolemia on Fatty Infiltration and Quality of Tendon-to-Bone Healing in a Rabbit Model of a Chronic Rotator Cuff Tear: Electrophysiological, Biomechanical, and Histological Analyses</title><title>The American journal of sports medicine</title><addtitle>Am J Sports Med</addtitle><description>Background:
The incidence of healing failure after rotator cuff repair is high, and fatty infiltration is a crucial factor in healing failure.
Purpose:
To verify the effect of hypercholesterolemia on fatty infiltration and the quality of tendon-to-bone healing and its reversibility by lowering the cholesterol level in a chronic tear model using the rabbit supraspinatus.
Study Design:
Controlled laboratory study.
Methods:
Forty-eight rabbits were randomly allocated into 4 groups (n = 12 each). After 4 weeks of a high-cholesterol diet (groups A and B) and a regular diet (groups C and D), the supraspinatus tendon was detached and left alone for 6 weeks and then was repaired in a transosseous manner (groups A, B, and C). Group D served as a control. Group A continued to receive the high-cholesterol diet until the final evaluation (6 weeks after repair); however, at the time of repair, group B was changed to a general diet with administration of a cholesterol-lowering agent (simvastatin). Histological evaluation of the fat-to-muscle proportion was performed twice, at the time of repair and the final evaluation, and an electromyographic (EMG) test, mechanical test, and histological test of tendon-to-bone healing were performed at the final evaluation.
Results:
For the EMG test, group A showed a significantly smaller area of compound muscle action potential compared with groups C and D (all P <.01), and group B showed a larger area than group A, almost up to the level of group C (P = .312). Similarly, group A showed significantly lower mechanical properties both in load-to-failure and stiffness compared with groups C and D (all P <.05). In addition, although not significantly different, the mechanical properties of group B were higher than those of group A (mean load-to-failure: group A = 42.01 N, group B = 58.23 N [P = .103]; mean stiffness: group A = 36.32 N/mm, group B = 47.22 N/mm [P = .153]). For the histological test, groups A and B showed a significantly higher fat-to-muscle proportion than did groups C and D at 6 weeks after detachment (all P <.05), but at the final evaluation, group B showed a decreased fat-to-muscle proportion (mean ± SD: from 64.02% ± 11.87% to 54.68% ± 10.47%; P = .146) compared with group A, which showed increased fat-to-muscle proportion (from 59.26% ± 17.80% to 78.23% ± 10.87%; P = .015). Groups B and C showed better tendon-to-bone interface structures than did group A, which showed coarse and poorly organized collagen fibers with fat interposition.
Conclusion:
Hypercholesterolemia had a deleterious effect on fatty infiltration and the quality of tendon-to-bone repair site, and lowering hypercholesterolemia seemed to halt or reverse these harmful effects in this experimental model.
Clinical Relevance:
Systemic diseases such as hypercholesterolemia should be tightly controlled during the perioperative period of rotator cuff repair.</description><subject>Animals</subject><subject>Biomechanics</subject><subject>Body fat</subject><subject>Bone and Bones - physiology</subject><subject>Diet</subject><subject>Disease Models, Animal</subject><subject>Fats - metabolism</subject><subject>Histology</subject><subject>Hypercholesterolemia - physiopathology</subject><subject>Male</subject><subject>Physiology</subject><subject>Rabbits</subject><subject>Random Allocation</subject><subject>Rotator Cuff Injuries - metabolism</subject><subject>Rotator Cuff Injuries - surgery</subject><subject>Tissue engineering</subject><subject>Wound Healing</subject><issn>0363-5465</issn><issn>1552-3365</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1rFTEYhYNY7G1170oCbtyM5mPyMUu9tN5CpVjqesgkb9opc5Nrklnc3-CfNsNtRQqFrl7Iec4Jh4PQe0o-U6rUF8IlF60UVEimNJWv0IoKwRrOpXiNVovcLPoxOsn5nhBCldRv0DGTHWVM8xX6c-Y92IKjx5v9DpK9ixPkAqme7WhwDPjclLLHF8GPU0mmjPXJBId_zmYaq1CdNxBcDE2JzbcYAG-gKuEWjxXE12YYxoJ_RAfTwhq8vksxjBZfx2JKTHg9-yXCpLfoyJspw7uHe4p-nZ_drDfN5dX3i_XXy8ZyQUtjBsUNbZXTZiCEScKpE1a1tZPtODgKngCjrRdME2cNeFdR3jGtFVOd5afo0yF3l-Lvubbtt2O2ME0mQJxzT1VHOtlKJV6Aas0YZ4RW9OMT9D7OKdQiSyBjlMu2qxQ5UDbFnBP4fpfGrUn7npJ-2bR_umm1fHgInoctuH-GxxEr0ByAbG7hv1-fC_wLAjGnug</recordid><startdate>20160501</startdate><enddate>20160501</enddate><creator>Chung, Seok Won</creator><creator>Park, HaeBong</creator><creator>Kwon, Jieun</creator><creator>Choe, Ghee Young</creator><creator>Kim, Sae Hoon</creator><creator>Oh, Joo Han</creator><general>SAGE Publications</general><general>Sage Publications Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TS</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>U9A</scope><scope>7X8</scope></search><sort><creationdate>20160501</creationdate><title>Effect of Hypercholesterolemia on Fatty Infiltration and Quality of Tendon-to-Bone Healing in a Rabbit Model of a Chronic Rotator Cuff Tear</title><author>Chung, Seok Won ; Park, HaeBong ; Kwon, Jieun ; Choe, Ghee Young ; Kim, Sae Hoon ; Oh, Joo Han</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c351t-ab73a147d8ab0026031d5c74691c93ed1ef0e214f5280dcaefdd8a392887279c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Biomechanics</topic><topic>Body fat</topic><topic>Bone and Bones - physiology</topic><topic>Diet</topic><topic>Disease Models, Animal</topic><topic>Fats - metabolism</topic><topic>Histology</topic><topic>Hypercholesterolemia - physiopathology</topic><topic>Male</topic><topic>Physiology</topic><topic>Rabbits</topic><topic>Random Allocation</topic><topic>Rotator Cuff Injuries - metabolism</topic><topic>Rotator Cuff Injuries - surgery</topic><topic>Tissue engineering</topic><topic>Wound Healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chung, Seok Won</creatorcontrib><creatorcontrib>Park, HaeBong</creatorcontrib><creatorcontrib>Kwon, Jieun</creatorcontrib><creatorcontrib>Choe, Ghee Young</creatorcontrib><creatorcontrib>Kim, Sae Hoon</creatorcontrib><creatorcontrib>Oh, Joo Han</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Physical Education Index</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>The American journal of sports medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chung, Seok Won</au><au>Park, HaeBong</au><au>Kwon, Jieun</au><au>Choe, Ghee Young</au><au>Kim, Sae Hoon</au><au>Oh, Joo Han</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Hypercholesterolemia on Fatty Infiltration and Quality of Tendon-to-Bone Healing in a Rabbit Model of a Chronic Rotator Cuff Tear: Electrophysiological, Biomechanical, and Histological Analyses</atitle><jtitle>The American journal of sports medicine</jtitle><addtitle>Am J Sports Med</addtitle><date>2016-05-01</date><risdate>2016</risdate><volume>44</volume><issue>5</issue><spage>1153</spage><epage>1164</epage><pages>1153-1164</pages><issn>0363-5465</issn><eissn>1552-3365</eissn><coden>AJSMDO</coden><abstract>Background:
The incidence of healing failure after rotator cuff repair is high, and fatty infiltration is a crucial factor in healing failure.
Purpose:
To verify the effect of hypercholesterolemia on fatty infiltration and the quality of tendon-to-bone healing and its reversibility by lowering the cholesterol level in a chronic tear model using the rabbit supraspinatus.
Study Design:
Controlled laboratory study.
Methods:
Forty-eight rabbits were randomly allocated into 4 groups (n = 12 each). After 4 weeks of a high-cholesterol diet (groups A and B) and a regular diet (groups C and D), the supraspinatus tendon was detached and left alone for 6 weeks and then was repaired in a transosseous manner (groups A, B, and C). Group D served as a control. Group A continued to receive the high-cholesterol diet until the final evaluation (6 weeks after repair); however, at the time of repair, group B was changed to a general diet with administration of a cholesterol-lowering agent (simvastatin). Histological evaluation of the fat-to-muscle proportion was performed twice, at the time of repair and the final evaluation, and an electromyographic (EMG) test, mechanical test, and histological test of tendon-to-bone healing were performed at the final evaluation.
Results:
For the EMG test, group A showed a significantly smaller area of compound muscle action potential compared with groups C and D (all P <.01), and group B showed a larger area than group A, almost up to the level of group C (P = .312). Similarly, group A showed significantly lower mechanical properties both in load-to-failure and stiffness compared with groups C and D (all P <.05). In addition, although not significantly different, the mechanical properties of group B were higher than those of group A (mean load-to-failure: group A = 42.01 N, group B = 58.23 N [P = .103]; mean stiffness: group A = 36.32 N/mm, group B = 47.22 N/mm [P = .153]). For the histological test, groups A and B showed a significantly higher fat-to-muscle proportion than did groups C and D at 6 weeks after detachment (all P <.05), but at the final evaluation, group B showed a decreased fat-to-muscle proportion (mean ± SD: from 64.02% ± 11.87% to 54.68% ± 10.47%; P = .146) compared with group A, which showed increased fat-to-muscle proportion (from 59.26% ± 17.80% to 78.23% ± 10.87%; P = .015). Groups B and C showed better tendon-to-bone interface structures than did group A, which showed coarse and poorly organized collagen fibers with fat interposition.
Conclusion:
Hypercholesterolemia had a deleterious effect on fatty infiltration and the quality of tendon-to-bone repair site, and lowering hypercholesterolemia seemed to halt or reverse these harmful effects in this experimental model.
Clinical Relevance:
Systemic diseases such as hypercholesterolemia should be tightly controlled during the perioperative period of rotator cuff repair.</abstract><cop>Los Angeles, CA</cop><pub>SAGE Publications</pub><pmid>26912283</pmid><doi>10.1177/0363546515627816</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0363-5465 |
| ispartof | The American journal of sports medicine, 2016-05, Vol.44 (5), p.1153-1164 |
| issn | 0363-5465 1552-3365 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1790964675 |
| source | MEDLINE; SAGE Complete A-Z List; Alma/SFX Local Collection |
| subjects | Animals Biomechanics Body fat Bone and Bones - physiology Diet Disease Models, Animal Fats - metabolism Histology Hypercholesterolemia - physiopathology Male Physiology Rabbits Random Allocation Rotator Cuff Injuries - metabolism Rotator Cuff Injuries - surgery Tissue engineering Wound Healing |
| title | Effect of Hypercholesterolemia on Fatty Infiltration and Quality of Tendon-to-Bone Healing in a Rabbit Model of a Chronic Rotator Cuff Tear: Electrophysiological, Biomechanical, and Histological Analyses |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T16%3A16%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20Hypercholesterolemia%20on%20Fatty%20Infiltration%20and%20Quality%20of%20Tendon-to-Bone%20Healing%20in%20a%20Rabbit%20Model%20of%20a%20Chronic%20Rotator%20Cuff%20Tear:%20Electrophysiological,%20Biomechanical,%20and%20Histological%20Analyses&rft.jtitle=The%20American%20journal%20of%20sports%20medicine&rft.au=Chung,%20Seok%20Won&rft.date=2016-05-01&rft.volume=44&rft.issue=5&rft.spage=1153&rft.epage=1164&rft.pages=1153-1164&rft.issn=0363-5465&rft.eissn=1552-3365&rft.coden=AJSMDO&rft_id=info:doi/10.1177/0363546515627816&rft_dat=%3Cproquest_cross%3E4070802211%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1792213649&rft_id=info:pmid/26912283&rft_sage_id=10.1177_0363546515627816&rfr_iscdi=true |