CRISPR/Cas9 advances engineering of microbial cell factories

One of the key drivers for successful metabolic engineering in microbes is the efficacy by which genomes can be edited. As such there are many methods to choose from when aiming to modify genomes, especially those of model organisms like yeast and bacteria. In recent years, clustered regularly inter...

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Veröffentlicht in:	Metabolic engineering 2016-03, Vol.34, p.44-59
Hauptverfasser:	Jakočiūnas, Tadas, Jensen, Michael K., Keasling, Jay D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria Clustered Regularly Interspaced Short Palindromic Repeats - genetics CRISPR-Associated Proteins - genetics CRISPR-Cas Systems - genetics CRISPR/Cas9 Genetic Enhancement - methods Genome editing Genome, Microbial - genetics Metabolic engineering Metabolic Engineering - methods Metabolic Networks and Pathways - genetics Recombineering Yeast
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