Breeding of high biomass and lipid producing Desmodesmus sp. by Ethylmethane sulfonate-induced mutation
•EMS mutation could be used for breeding of Desmodesmus sp. successfully.•High biomass and lipid production mutants S5 and G3 were obtained.•The cells of mutant strains had higher contents of MUFAs and neutral lipids.•The mutant strains had higher stability for producing biomass and lipid. To improv...
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| Veröffentlicht in: | Bioresource technology 2016-05, Vol.207, p.268-275 |
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| creator | Zhang, Yi He, Meilin Zou, Shanmei Fei, Cong Yan, Yongquan Zheng, Shiyan Rajper, Aftab Ahmed Wang, Changhai |
| description | •EMS mutation could be used for breeding of Desmodesmus sp. successfully.•High biomass and lipid production mutants S5 and G3 were obtained.•The cells of mutant strains had higher contents of MUFAs and neutral lipids.•The mutant strains had higher stability for producing biomass and lipid.
To improve the biomass yield and lipid productivity, two desert microalgae, Desmodesmus sp. S81 and G41 were induced mutagenesis by Ethylmethane sulfonate (EMS), and obtained two potential mutants, Desmodesmus sp. S5 and G3 from the mutagenic clones for their greatly promoted biomass and lipid production. The results showed that the biomass yield, lipid content and lipid productivity of the mutant strains S5 and G3 were 778.10mg·L−1, 48.41% and 19.83mg·L−1·d−1, 739.52mg·L−1, 46.01%, and 17.92mg·L−1·d−1, respectively, which presented the increment of 45.50%, 8.00% and 74.24%, 20.67%, 10.35% and 55.77% than those of S81 and G41. Comparing with the wild strains, the mutants showed reduced PUFAs and glycol lipids, elevated MUFAs and neutral lipids contents, which were appropriate for biodiesel production. |
| doi_str_mv | 10.1016/j.biortech.2016.01.120 |
| format | Article |
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To improve the biomass yield and lipid productivity, two desert microalgae, Desmodesmus sp. S81 and G41 were induced mutagenesis by Ethylmethane sulfonate (EMS), and obtained two potential mutants, Desmodesmus sp. S5 and G3 from the mutagenic clones for their greatly promoted biomass and lipid production. The results showed that the biomass yield, lipid content and lipid productivity of the mutant strains S5 and G3 were 778.10mg·L−1, 48.41% and 19.83mg·L−1·d−1, 739.52mg·L−1, 46.01%, and 17.92mg·L−1·d−1, respectively, which presented the increment of 45.50%, 8.00% and 74.24%, 20.67%, 10.35% and 55.77% than those of S81 and G41. Comparing with the wild strains, the mutants showed reduced PUFAs and glycol lipids, elevated MUFAs and neutral lipids contents, which were appropriate for biodiesel production.</description><identifier>ISSN: 0960-8524</identifier><identifier>EISSN: 1873-2976</identifier><identifier>DOI: 10.1016/j.biortech.2016.01.120</identifier><identifier>PMID: 26894567</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Biofuels - microbiology ; Biomass ; Cell Culture Techniques - methods ; Chlorophyll - metabolism ; Chlorophyta - drug effects ; Chlorophyta - genetics ; Chlorophyta - growth & development ; Chlorophyta - metabolism ; Desmodesmus ; Ethyl Methanesulfonate - pharmacology ; Ethylmethane sulfonate ; Fatty Acids - metabolism ; Fluorescence ; Lipid ; Lipids - biosynthesis ; Microalgae - genetics ; Microalgae - growth & development ; Microalgae - metabolism ; Mutagenesis ; Mutation ; Mutation - genetics ; Oxazines - metabolism ; Time Factors</subject><ispartof>Bioresource technology, 2016-05, Vol.207, p.268-275</ispartof><rights>2016 Elsevier Ltd</rights><rights>Copyright © 2016 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c401t-bb278ddf584cf1bd53ee634d0380164d4ffb4628fe26233f554e4aa2e3d6d6cb3</citedby><cites>FETCH-LOGICAL-c401t-bb278ddf584cf1bd53ee634d0380164d4ffb4628fe26233f554e4aa2e3d6d6cb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.biortech.2016.01.120$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26894567$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Yi</creatorcontrib><creatorcontrib>He, Meilin</creatorcontrib><creatorcontrib>Zou, Shanmei</creatorcontrib><creatorcontrib>Fei, Cong</creatorcontrib><creatorcontrib>Yan, Yongquan</creatorcontrib><creatorcontrib>Zheng, Shiyan</creatorcontrib><creatorcontrib>Rajper, Aftab Ahmed</creatorcontrib><creatorcontrib>Wang, Changhai</creatorcontrib><title>Breeding of high biomass and lipid producing Desmodesmus sp. by Ethylmethane sulfonate-induced mutation</title><title>Bioresource technology</title><addtitle>Bioresour Technol</addtitle><description>•EMS mutation could be used for breeding of Desmodesmus sp. successfully.•High biomass and lipid production mutants S5 and G3 were obtained.•The cells of mutant strains had higher contents of MUFAs and neutral lipids.•The mutant strains had higher stability for producing biomass and lipid.
To improve the biomass yield and lipid productivity, two desert microalgae, Desmodesmus sp. S81 and G41 were induced mutagenesis by Ethylmethane sulfonate (EMS), and obtained two potential mutants, Desmodesmus sp. S5 and G3 from the mutagenic clones for their greatly promoted biomass and lipid production. The results showed that the biomass yield, lipid content and lipid productivity of the mutant strains S5 and G3 were 778.10mg·L−1, 48.41% and 19.83mg·L−1·d−1, 739.52mg·L−1, 46.01%, and 17.92mg·L−1·d−1, respectively, which presented the increment of 45.50%, 8.00% and 74.24%, 20.67%, 10.35% and 55.77% than those of S81 and G41. Comparing with the wild strains, the mutants showed reduced PUFAs and glycol lipids, elevated MUFAs and neutral lipids contents, which were appropriate for biodiesel production.</description><subject>Biofuels - microbiology</subject><subject>Biomass</subject><subject>Cell Culture Techniques - methods</subject><subject>Chlorophyll - metabolism</subject><subject>Chlorophyta - drug effects</subject><subject>Chlorophyta - genetics</subject><subject>Chlorophyta - growth & development</subject><subject>Chlorophyta - metabolism</subject><subject>Desmodesmus</subject><subject>Ethyl Methanesulfonate - pharmacology</subject><subject>Ethylmethane sulfonate</subject><subject>Fatty Acids - metabolism</subject><subject>Fluorescence</subject><subject>Lipid</subject><subject>Lipids - biosynthesis</subject><subject>Microalgae - genetics</subject><subject>Microalgae - growth & development</subject><subject>Microalgae - metabolism</subject><subject>Mutagenesis</subject><subject>Mutation</subject><subject>Mutation - genetics</subject><subject>Oxazines - metabolism</subject><subject>Time Factors</subject><issn>0960-8524</issn><issn>1873-2976</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtu2zAQRYmgReOk_YWAy26k8CVK3iVNnAcQIJt2TVDk0KIhiQ5JFfDfh4aTbruZwQD3zsw9CF1RUlNC5fWu7n2IGcxQszLXhNaUkTO0ol3LK7Zu5Re0ImtJqq5h4hxdpLQjhHDasm_onMluLRrZrtD2VwSwft7i4PDgtwMueyedEtazxaPfe4v3MdjFHDX3kKZgS1kSTvsa9we8ycNhnCAPegacltGFWWeo_FwsYPG0ZJ19mL-jr06PCX589Ev052Hz--6penl9fL67famMIDRXfc_azlrXdMI42tuGA0guLOFdSSmscK4XknUOmGScu6YRILRmwK200vT8Ev087S1Pvy2Qspp8MjCO5b2wJEXbNSnRiWBFKk9SE0NKEZzaRz_peFCUqCNktVOfkNURsiJUFcjFePVxY-knsP9sn1SL4OYkgJL0r4eokvEwFx4-gsnKBv-_G--HxJMZ</recordid><startdate>201605</startdate><enddate>201605</enddate><creator>Zhang, Yi</creator><creator>He, Meilin</creator><creator>Zou, Shanmei</creator><creator>Fei, Cong</creator><creator>Yan, Yongquan</creator><creator>Zheng, Shiyan</creator><creator>Rajper, Aftab Ahmed</creator><creator>Wang, Changhai</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7ST</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>P64</scope><scope>SOI</scope></search><sort><creationdate>201605</creationdate><title>Breeding of high biomass and lipid producing Desmodesmus sp. by Ethylmethane sulfonate-induced mutation</title><author>Zhang, Yi ; He, Meilin ; Zou, Shanmei ; Fei, Cong ; Yan, Yongquan ; Zheng, Shiyan ; Rajper, Aftab Ahmed ; Wang, Changhai</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c401t-bb278ddf584cf1bd53ee634d0380164d4ffb4628fe26233f554e4aa2e3d6d6cb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Biofuels - microbiology</topic><topic>Biomass</topic><topic>Cell Culture Techniques - methods</topic><topic>Chlorophyll - metabolism</topic><topic>Chlorophyta - drug effects</topic><topic>Chlorophyta - genetics</topic><topic>Chlorophyta - growth & development</topic><topic>Chlorophyta - metabolism</topic><topic>Desmodesmus</topic><topic>Ethyl Methanesulfonate - pharmacology</topic><topic>Ethylmethane sulfonate</topic><topic>Fatty Acids - metabolism</topic><topic>Fluorescence</topic><topic>Lipid</topic><topic>Lipids - biosynthesis</topic><topic>Microalgae - genetics</topic><topic>Microalgae - growth & development</topic><topic>Microalgae - metabolism</topic><topic>Mutagenesis</topic><topic>Mutation</topic><topic>Mutation - genetics</topic><topic>Oxazines - metabolism</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Yi</creatorcontrib><creatorcontrib>He, Meilin</creatorcontrib><creatorcontrib>Zou, Shanmei</creatorcontrib><creatorcontrib>Fei, Cong</creatorcontrib><creatorcontrib>Yan, Yongquan</creatorcontrib><creatorcontrib>Zheng, Shiyan</creatorcontrib><creatorcontrib>Rajper, Aftab Ahmed</creatorcontrib><creatorcontrib>Wang, Changhai</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Environment Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Bioresource technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Yi</au><au>He, Meilin</au><au>Zou, Shanmei</au><au>Fei, Cong</au><au>Yan, Yongquan</au><au>Zheng, Shiyan</au><au>Rajper, Aftab Ahmed</au><au>Wang, Changhai</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Breeding of high biomass and lipid producing Desmodesmus sp. by Ethylmethane sulfonate-induced mutation</atitle><jtitle>Bioresource technology</jtitle><addtitle>Bioresour Technol</addtitle><date>2016-05</date><risdate>2016</risdate><volume>207</volume><spage>268</spage><epage>275</epage><pages>268-275</pages><issn>0960-8524</issn><eissn>1873-2976</eissn><abstract>•EMS mutation could be used for breeding of Desmodesmus sp. successfully.•High biomass and lipid production mutants S5 and G3 were obtained.•The cells of mutant strains had higher contents of MUFAs and neutral lipids.•The mutant strains had higher stability for producing biomass and lipid.
To improve the biomass yield and lipid productivity, two desert microalgae, Desmodesmus sp. S81 and G41 were induced mutagenesis by Ethylmethane sulfonate (EMS), and obtained two potential mutants, Desmodesmus sp. S5 and G3 from the mutagenic clones for their greatly promoted biomass and lipid production. The results showed that the biomass yield, lipid content and lipid productivity of the mutant strains S5 and G3 were 778.10mg·L−1, 48.41% and 19.83mg·L−1·d−1, 739.52mg·L−1, 46.01%, and 17.92mg·L−1·d−1, respectively, which presented the increment of 45.50%, 8.00% and 74.24%, 20.67%, 10.35% and 55.77% than those of S81 and G41. Comparing with the wild strains, the mutants showed reduced PUFAs and glycol lipids, elevated MUFAs and neutral lipids contents, which were appropriate for biodiesel production.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>26894567</pmid><doi>10.1016/j.biortech.2016.01.120</doi><tpages>8</tpages></addata></record> |
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| subjects | Biofuels - microbiology Biomass Cell Culture Techniques - methods Chlorophyll - metabolism Chlorophyta - drug effects Chlorophyta - genetics Chlorophyta - growth & development Chlorophyta - metabolism Desmodesmus Ethyl Methanesulfonate - pharmacology Ethylmethane sulfonate Fatty Acids - metabolism Fluorescence Lipid Lipids - biosynthesis Microalgae - genetics Microalgae - growth & development Microalgae - metabolism Mutagenesis Mutation Mutation - genetics Oxazines - metabolism Time Factors |
| title | Breeding of high biomass and lipid producing Desmodesmus sp. by Ethylmethane sulfonate-induced mutation |
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