Modelling affective pain in mice: Effects of inflammatory hypersensitivity on place escape/avoidance behaviour, anxiety and hedonic state
•Affective components of pain are not reflected by traditional pain assessment methods.•This mouse place escape/avoidance paradigm may be used to investigate affective pain.•Inflammatory hypersensitivity is associated with anxiety-like behaviour in mice.•No correlation between affective pain behavio...
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| Veröffentlicht in: | Journal of neuroscience methods 2016-03, Vol.262, p.85-92 |
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| creator | Refsgaard, L.K. Hoffmann-Petersen, J. Sahlholt, M. Pickering, D.S. Andreasen, J.T. |
| description | •Affective components of pain are not reflected by traditional pain assessment methods.•This mouse place escape/avoidance paradigm may be used to investigate affective pain.•Inflammatory hypersensitivity is associated with anxiety-like behaviour in mice.•No correlation between affective pain behaviour and possible confounding factors.
The place escape/avoidance paradigm (PEAP) has been used to assess the affective component of pain in rats. Using the Complete Freund's Adjuvant (CFA) model of inflammatory pain, the current study aimed at developing a mouse version of PEAP and investigating the relation between PEAP and other behavioural responses, namely anxiety-like behaviour, locomotor activity, and hedonic state.
A novel paradigm assessing the affective component of pain in mice was developed by modifying the setup known from rat studies: Animals were forced to stay 2×5min in the light and the dark area of a box while being stimulated with a suprathreshold filament on the untreated or treated paw, respectively. This was followed by a 30-min test with unrestricted movement. Anxiety-like behaviour, locomotor activity, and hedonic state were assessed with the elevated zero maze (EZM), an open field setup, and a saccharin preference test, respectively, and correlated with the PEAP behaviour to examine potentially confounding parameters of the novel paradigm.
In the PEAP, CFA-treated animals spent more time in the light area. CFA also increased anxiety-like behaviour significantly, whereas locomotor activity was unaffected. A significant, albeit modest, reduction in saccharin preference was observed. PEAP responses showed no significant correlations with any other behavioural measure.
The PEAP results suggest that this paradigm might be successfully applied in mice to study affective pain. CFA treatment was associated with increased anxiety-like behaviour and anhedonia; however, this appeared unrelated to the PEAP responses. |
| doi_str_mv | 10.1016/j.jneumeth.2016.01.019 |
| format | Article |
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The place escape/avoidance paradigm (PEAP) has been used to assess the affective component of pain in rats. Using the Complete Freund's Adjuvant (CFA) model of inflammatory pain, the current study aimed at developing a mouse version of PEAP and investigating the relation between PEAP and other behavioural responses, namely anxiety-like behaviour, locomotor activity, and hedonic state.
A novel paradigm assessing the affective component of pain in mice was developed by modifying the setup known from rat studies: Animals were forced to stay 2×5min in the light and the dark area of a box while being stimulated with a suprathreshold filament on the untreated or treated paw, respectively. This was followed by a 30-min test with unrestricted movement. Anxiety-like behaviour, locomotor activity, and hedonic state were assessed with the elevated zero maze (EZM), an open field setup, and a saccharin preference test, respectively, and correlated with the PEAP behaviour to examine potentially confounding parameters of the novel paradigm.
In the PEAP, CFA-treated animals spent more time in the light area. CFA also increased anxiety-like behaviour significantly, whereas locomotor activity was unaffected. A significant, albeit modest, reduction in saccharin preference was observed. PEAP responses showed no significant correlations with any other behavioural measure.
The PEAP results suggest that this paradigm might be successfully applied in mice to study affective pain. CFA treatment was associated with increased anxiety-like behaviour and anhedonia; however, this appeared unrelated to the PEAP responses.</description><identifier>ISSN: 0165-0270</identifier><identifier>EISSN: 1872-678X</identifier><identifier>DOI: 10.1016/j.jneumeth.2016.01.019</identifier><identifier>PMID: 26820902</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Affective pain ; Analysis of Variance ; Anhedonia ; Animals ; Anxiety ; Anxiety Disorders - diagnosis ; Anxiety Disorders - etiology ; Avoidance behaviour ; Avoidance Learning - physiology ; CFA ; Disease Models, Animal ; Female ; Food Preferences ; Freund's Adjuvant - toxicity ; Hyperalgesia - physiopathology ; Inflammation - chemically induced ; Inflammation - complications ; Locomotion - drug effects ; Locomotion - physiology ; Maze Learning - physiology ; Mice ; Mice, Inbred C57BL ; Pain - diagnosis ; Pain - etiology ; Pain - psychology ; Pain Measurement ; Pain Threshold - physiology ; Saccharin - administration & dosage ; Sweetening Agents - administration & dosage ; Time Factors</subject><ispartof>Journal of neuroscience methods, 2016-03, Vol.262, p.85-92</ispartof><rights>2016 Elsevier B.V.</rights><rights>Copyright © 2016 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c401t-82a234baaabf5cafd2969e0c5e6039c3c7b66bfe9d9c2935f4c4ce3a753f13b93</citedby><cites>FETCH-LOGICAL-c401t-82a234baaabf5cafd2969e0c5e6039c3c7b66bfe9d9c2935f4c4ce3a753f13b93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0165027016000364$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26820902$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Refsgaard, L.K.</creatorcontrib><creatorcontrib>Hoffmann-Petersen, J.</creatorcontrib><creatorcontrib>Sahlholt, M.</creatorcontrib><creatorcontrib>Pickering, D.S.</creatorcontrib><creatorcontrib>Andreasen, J.T.</creatorcontrib><title>Modelling affective pain in mice: Effects of inflammatory hypersensitivity on place escape/avoidance behaviour, anxiety and hedonic state</title><title>Journal of neuroscience methods</title><addtitle>J Neurosci Methods</addtitle><description>•Affective components of pain are not reflected by traditional pain assessment methods.•This mouse place escape/avoidance paradigm may be used to investigate affective pain.•Inflammatory hypersensitivity is associated with anxiety-like behaviour in mice.•No correlation between affective pain behaviour and possible confounding factors.
The place escape/avoidance paradigm (PEAP) has been used to assess the affective component of pain in rats. Using the Complete Freund's Adjuvant (CFA) model of inflammatory pain, the current study aimed at developing a mouse version of PEAP and investigating the relation between PEAP and other behavioural responses, namely anxiety-like behaviour, locomotor activity, and hedonic state.
A novel paradigm assessing the affective component of pain in mice was developed by modifying the setup known from rat studies: Animals were forced to stay 2×5min in the light and the dark area of a box while being stimulated with a suprathreshold filament on the untreated or treated paw, respectively. This was followed by a 30-min test with unrestricted movement. Anxiety-like behaviour, locomotor activity, and hedonic state were assessed with the elevated zero maze (EZM), an open field setup, and a saccharin preference test, respectively, and correlated with the PEAP behaviour to examine potentially confounding parameters of the novel paradigm.
In the PEAP, CFA-treated animals spent more time in the light area. CFA also increased anxiety-like behaviour significantly, whereas locomotor activity was unaffected. A significant, albeit modest, reduction in saccharin preference was observed. PEAP responses showed no significant correlations with any other behavioural measure.
The PEAP results suggest that this paradigm might be successfully applied in mice to study affective pain. CFA treatment was associated with increased anxiety-like behaviour and anhedonia; however, this appeared unrelated to the PEAP responses.</description><subject>Affective pain</subject><subject>Analysis of Variance</subject><subject>Anhedonia</subject><subject>Animals</subject><subject>Anxiety</subject><subject>Anxiety Disorders - diagnosis</subject><subject>Anxiety Disorders - etiology</subject><subject>Avoidance behaviour</subject><subject>Avoidance Learning - physiology</subject><subject>CFA</subject><subject>Disease Models, Animal</subject><subject>Female</subject><subject>Food Preferences</subject><subject>Freund's Adjuvant - toxicity</subject><subject>Hyperalgesia - physiopathology</subject><subject>Inflammation - chemically induced</subject><subject>Inflammation - complications</subject><subject>Locomotion - drug effects</subject><subject>Locomotion - physiology</subject><subject>Maze Learning - physiology</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Pain - diagnosis</subject><subject>Pain - etiology</subject><subject>Pain - psychology</subject><subject>Pain Measurement</subject><subject>Pain Threshold - physiology</subject><subject>Saccharin - administration & dosage</subject><subject>Sweetening Agents - administration & dosage</subject><subject>Time Factors</subject><issn>0165-0270</issn><issn>1872-678X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc9u1DAQxi0EokvhFSofOZCt7SROzAlUlT9SEReQuFkTe8x6ldjBTlbsI_DWeNmWK0gjWf78mxnr-wi54mzLGZfX--0-4DrhstuKct8yXko9Ihved6KSXf_tMdmUh7ZiomMX5FnOe8ZYo5h8Si6E7AVTTGzIr0_R4jj68J2Cc2gWf0A6gw-01OQNvqa3f_RMoyuaG2GaYInpSHfHGVPGkH1p8suRxkDnEQxSzAZmvIZD9BZCEQbcwcHHNb2iEH56LDAES3doY_CG5gUWfE6eOBgzvrg_L8nXd7dfbj5Ud5_ff7x5e1eZhvGl6gWIuhkAYHCtAWeFkgqZaVGyWpnadIOUg0NllRGqbl1jGoM1dG3teD2o-pK8PM-dU_yxYl705LMpHkDAuGbNO8VU0zRt_x-oVJL3hS2oPKMmxZwTOj0nP0E6as70KTG91w-J6VNimvFSp-9c3e9Yhwnt37aHiArw5gxgMeXgMelsPBZXrU8lF22j_9eO3zQ1rno</recordid><startdate>20160315</startdate><enddate>20160315</enddate><creator>Refsgaard, L.K.</creator><creator>Hoffmann-Petersen, J.</creator><creator>Sahlholt, M.</creator><creator>Pickering, D.S.</creator><creator>Andreasen, J.T.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TK</scope></search><sort><creationdate>20160315</creationdate><title>Modelling affective pain in mice: Effects of inflammatory hypersensitivity on place escape/avoidance behaviour, anxiety and hedonic state</title><author>Refsgaard, L.K. ; Hoffmann-Petersen, J. ; Sahlholt, M. ; Pickering, D.S. ; Andreasen, J.T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c401t-82a234baaabf5cafd2969e0c5e6039c3c7b66bfe9d9c2935f4c4ce3a753f13b93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Affective pain</topic><topic>Analysis of Variance</topic><topic>Anhedonia</topic><topic>Animals</topic><topic>Anxiety</topic><topic>Anxiety Disorders - diagnosis</topic><topic>Anxiety Disorders - etiology</topic><topic>Avoidance behaviour</topic><topic>Avoidance Learning - physiology</topic><topic>CFA</topic><topic>Disease Models, Animal</topic><topic>Female</topic><topic>Food Preferences</topic><topic>Freund's Adjuvant - toxicity</topic><topic>Hyperalgesia - physiopathology</topic><topic>Inflammation - chemically induced</topic><topic>Inflammation - complications</topic><topic>Locomotion - drug effects</topic><topic>Locomotion - physiology</topic><topic>Maze Learning - physiology</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Pain - diagnosis</topic><topic>Pain - etiology</topic><topic>Pain - psychology</topic><topic>Pain Measurement</topic><topic>Pain Threshold - physiology</topic><topic>Saccharin - administration & dosage</topic><topic>Sweetening Agents - administration & dosage</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Refsgaard, L.K.</creatorcontrib><creatorcontrib>Hoffmann-Petersen, J.</creatorcontrib><creatorcontrib>Sahlholt, M.</creatorcontrib><creatorcontrib>Pickering, D.S.</creatorcontrib><creatorcontrib>Andreasen, J.T.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Neurosciences Abstracts</collection><jtitle>Journal of neuroscience methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Refsgaard, L.K.</au><au>Hoffmann-Petersen, J.</au><au>Sahlholt, M.</au><au>Pickering, D.S.</au><au>Andreasen, J.T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modelling affective pain in mice: Effects of inflammatory hypersensitivity on place escape/avoidance behaviour, anxiety and hedonic state</atitle><jtitle>Journal of neuroscience methods</jtitle><addtitle>J Neurosci Methods</addtitle><date>2016-03-15</date><risdate>2016</risdate><volume>262</volume><spage>85</spage><epage>92</epage><pages>85-92</pages><issn>0165-0270</issn><eissn>1872-678X</eissn><abstract>•Affective components of pain are not reflected by traditional pain assessment methods.•This mouse place escape/avoidance paradigm may be used to investigate affective pain.•Inflammatory hypersensitivity is associated with anxiety-like behaviour in mice.•No correlation between affective pain behaviour and possible confounding factors.
The place escape/avoidance paradigm (PEAP) has been used to assess the affective component of pain in rats. Using the Complete Freund's Adjuvant (CFA) model of inflammatory pain, the current study aimed at developing a mouse version of PEAP and investigating the relation between PEAP and other behavioural responses, namely anxiety-like behaviour, locomotor activity, and hedonic state.
A novel paradigm assessing the affective component of pain in mice was developed by modifying the setup known from rat studies: Animals were forced to stay 2×5min in the light and the dark area of a box while being stimulated with a suprathreshold filament on the untreated or treated paw, respectively. This was followed by a 30-min test with unrestricted movement. Anxiety-like behaviour, locomotor activity, and hedonic state were assessed with the elevated zero maze (EZM), an open field setup, and a saccharin preference test, respectively, and correlated with the PEAP behaviour to examine potentially confounding parameters of the novel paradigm.
In the PEAP, CFA-treated animals spent more time in the light area. CFA also increased anxiety-like behaviour significantly, whereas locomotor activity was unaffected. A significant, albeit modest, reduction in saccharin preference was observed. PEAP responses showed no significant correlations with any other behavioural measure.
The PEAP results suggest that this paradigm might be successfully applied in mice to study affective pain. CFA treatment was associated with increased anxiety-like behaviour and anhedonia; however, this appeared unrelated to the PEAP responses.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>26820902</pmid><doi>10.1016/j.jneumeth.2016.01.019</doi><tpages>8</tpages></addata></record> |
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| subjects | Affective pain Analysis of Variance Anhedonia Animals Anxiety Anxiety Disorders - diagnosis Anxiety Disorders - etiology Avoidance behaviour Avoidance Learning - physiology CFA Disease Models, Animal Female Food Preferences Freund's Adjuvant - toxicity Hyperalgesia - physiopathology Inflammation - chemically induced Inflammation - complications Locomotion - drug effects Locomotion - physiology Maze Learning - physiology Mice Mice, Inbred C57BL Pain - diagnosis Pain - etiology Pain - psychology Pain Measurement Pain Threshold - physiology Saccharin - administration & dosage Sweetening Agents - administration & dosage Time Factors |
| title | Modelling affective pain in mice: Effects of inflammatory hypersensitivity on place escape/avoidance behaviour, anxiety and hedonic state |
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