Performance and microbial community variations in thermophilic anaerobic digesters treating OTC medicated cow manure under different operational conditions
•Initial concentration of 2mg/L OTC caused 10–30% inhibition on biogas yields.•Higher inhibition was observed as mixing rate increased.•OTC half-life was calculated between 13 and 19days.•Effect of OTC was more pronounced on microbial diversity than operational conditions.•Methanogenesis was mostly...
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| Veröffentlicht in: | Bioresource technology 2016-04, Vol.205, p.191-198 |
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| creator | Akyol, Çağrı Turker, Gokhan Ince, Orhan Ertekin, Emine Üstüner, Oya Ince, Bahar |
| description | •Initial concentration of 2mg/L OTC caused 10–30% inhibition on biogas yields.•Higher inhibition was observed as mixing rate increased.•OTC half-life was calculated between 13 and 19days.•Effect of OTC was more pronounced on microbial diversity than operational conditions.•Methanogenesis was mostly through H2 and CO2 utilization.
This study aimed to determine the fate and effect of oxytetracycline (OTC) and its metabolites during thermophilic anaerobic digestion of cow manure. OTC-medicated and non-medicated digesters were operated at 55°C with different volatile solids (VS) concentrations (4% and 6%) and mixing rates (90 and 120rpm). OTC and its metabolites were measured by HPLC and LC/MS/MS, respectively. Microbial community dynamics were monitored by denaturing gradient gel electrophoresis (DGGE) and real-time PCR (qPCR). Approximately 2mg/L initial OTC concentration caused 10–30% inhibition on biogas production and higher inhibition was observed as mixing rate increased. DGGE results indicated that OTC caused a shift in bacterial community structure and several species became dominant with time. Archaeal community decreased throughout the digestion period. RNA based qPCR analyses showed that gene copy numbers of bacteria and Methanomicrobiales declined in all digesters whereas gene copy numbers of Methanobacteriales and Methanosarcinales increased in high mixing rate digesters. |
| doi_str_mv | 10.1016/j.biortech.2016.01.071 |
| format | Article |
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This study aimed to determine the fate and effect of oxytetracycline (OTC) and its metabolites during thermophilic anaerobic digestion of cow manure. OTC-medicated and non-medicated digesters were operated at 55°C with different volatile solids (VS) concentrations (4% and 6%) and mixing rates (90 and 120rpm). OTC and its metabolites were measured by HPLC and LC/MS/MS, respectively. Microbial community dynamics were monitored by denaturing gradient gel electrophoresis (DGGE) and real-time PCR (qPCR). Approximately 2mg/L initial OTC concentration caused 10–30% inhibition on biogas production and higher inhibition was observed as mixing rate increased. DGGE results indicated that OTC caused a shift in bacterial community structure and several species became dominant with time. Archaeal community decreased throughout the digestion period. RNA based qPCR analyses showed that gene copy numbers of bacteria and Methanomicrobiales declined in all digesters whereas gene copy numbers of Methanobacteriales and Methanosarcinales increased in high mixing rate digesters.</description><identifier>ISSN: 0960-8524</identifier><identifier>EISSN: 1873-2976</identifier><identifier>DOI: 10.1016/j.biortech.2016.01.071</identifier><identifier>PMID: 26826959</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Anaerobiosis ; Animals ; Anti-Bacterial Agents - analysis ; Anti-Bacterial Agents - metabolism ; Anti-Bacterial Agents - pharmacology ; Archaea - drug effects ; Archaea - genetics ; Archaea - physiology ; Biofuels ; Bioreactors - microbiology ; Carbon Dioxide - metabolism ; Cattle ; Chromatography, High Pressure Liquid ; Cow manure ; Denaturing Gradient Gel Electrophoresis ; DGGE ; Female ; Half-Life ; Hydrogen - metabolism ; Manure - microbiology ; Methanomicrobiales ; Methanomicrobiales - genetics ; Methanomicrobiales - physiology ; Methanosarcinales ; Methanosarcinales - drug effects ; Methanosarcinales - metabolism ; Methanosarcinales - physiology ; Microbial Consortia - drug effects ; Microbial Consortia - genetics ; Microbial Consortia - physiology ; Oxytetracycline ; Oxytetracycline - analysis ; Oxytetracycline - metabolism ; Oxytetracycline - pharmacology ; Real-time PCR ; Real-Time Polymerase Chain Reaction ; Tandem Mass Spectrometry ; Thermophilic anaerobic digestion</subject><ispartof>Bioresource technology, 2016-04, Vol.205, p.191-198</ispartof><rights>2016 Elsevier Ltd</rights><rights>Copyright © 2016 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c467t-491dd6a9ab48d7cc4f3ed00571515025eb770e441f57511a00d8b455874f6c453</citedby><cites>FETCH-LOGICAL-c467t-491dd6a9ab48d7cc4f3ed00571515025eb770e441f57511a00d8b455874f6c453</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.biortech.2016.01.071$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26826959$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Akyol, Çağrı</creatorcontrib><creatorcontrib>Turker, Gokhan</creatorcontrib><creatorcontrib>Ince, Orhan</creatorcontrib><creatorcontrib>Ertekin, Emine</creatorcontrib><creatorcontrib>Üstüner, Oya</creatorcontrib><creatorcontrib>Ince, Bahar</creatorcontrib><title>Performance and microbial community variations in thermophilic anaerobic digesters treating OTC medicated cow manure under different operational conditions</title><title>Bioresource technology</title><addtitle>Bioresour Technol</addtitle><description>•Initial concentration of 2mg/L OTC caused 10–30% inhibition on biogas yields.•Higher inhibition was observed as mixing rate increased.•OTC half-life was calculated between 13 and 19days.•Effect of OTC was more pronounced on microbial diversity than operational conditions.•Methanogenesis was mostly through H2 and CO2 utilization.
This study aimed to determine the fate and effect of oxytetracycline (OTC) and its metabolites during thermophilic anaerobic digestion of cow manure. OTC-medicated and non-medicated digesters were operated at 55°C with different volatile solids (VS) concentrations (4% and 6%) and mixing rates (90 and 120rpm). OTC and its metabolites were measured by HPLC and LC/MS/MS, respectively. Microbial community dynamics were monitored by denaturing gradient gel electrophoresis (DGGE) and real-time PCR (qPCR). Approximately 2mg/L initial OTC concentration caused 10–30% inhibition on biogas production and higher inhibition was observed as mixing rate increased. DGGE results indicated that OTC caused a shift in bacterial community structure and several species became dominant with time. Archaeal community decreased throughout the digestion period. RNA based qPCR analyses showed that gene copy numbers of bacteria and Methanomicrobiales declined in all digesters whereas gene copy numbers of Methanobacteriales and Methanosarcinales increased in high mixing rate digesters.</description><subject>Anaerobiosis</subject><subject>Animals</subject><subject>Anti-Bacterial Agents - analysis</subject><subject>Anti-Bacterial Agents - metabolism</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Archaea - drug effects</subject><subject>Archaea - genetics</subject><subject>Archaea - physiology</subject><subject>Biofuels</subject><subject>Bioreactors - microbiology</subject><subject>Carbon Dioxide - metabolism</subject><subject>Cattle</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Cow manure</subject><subject>Denaturing Gradient Gel Electrophoresis</subject><subject>DGGE</subject><subject>Female</subject><subject>Half-Life</subject><subject>Hydrogen - metabolism</subject><subject>Manure - microbiology</subject><subject>Methanomicrobiales</subject><subject>Methanomicrobiales - genetics</subject><subject>Methanomicrobiales - physiology</subject><subject>Methanosarcinales</subject><subject>Methanosarcinales - drug effects</subject><subject>Methanosarcinales - metabolism</subject><subject>Methanosarcinales - physiology</subject><subject>Microbial Consortia - drug effects</subject><subject>Microbial Consortia - genetics</subject><subject>Microbial Consortia - physiology</subject><subject>Oxytetracycline</subject><subject>Oxytetracycline - analysis</subject><subject>Oxytetracycline - metabolism</subject><subject>Oxytetracycline - pharmacology</subject><subject>Real-time PCR</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Tandem Mass Spectrometry</subject><subject>Thermophilic anaerobic digestion</subject><issn>0960-8524</issn><issn>1873-2976</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu3CAQhlHUqtmmfYWIYy92By8Y-9Zq1SaRIqWH9IwwjLOsbNgATpVn6cuG3U167Qkh_f98mvkIuWRQM2Dt1109uBAzmm3dlH8NrAbJzsiKdXJdNb1s35EV9C1UnWj4OfmY0g4A1kw2H8h503ZN24t-Rf7-wjiGOGtvkGpv6exMDIPTEzVhnhfv8jN90tHp7IJP1HmatxjnsN-6yZlS0XjIG2rdA6aMMdEcsaT9A72739AZrTM6oy3z_tDCWSLSxVuMpTGOGNFnGvYYj4Aj1lt3hH0i70c9Jfz8-l6Q3z9_3G-uq9u7q5vN99vK8FbmivfM2lb3euCdlcbwcY0WQEgmmIBG4CAlIOdsFFIwpgFsN3AhOsnH1nCxviBfTnP3MTwuZQk1u2RwmrTHsCTFZA89bxgcou0pWo6UUsRR7aObdXxWDNRBjNqpNzHqIEYBU0VMKV6-MpahnORf7c1ECXw7BbBs-uQwqmQcFivWRTRZ2eD-x3gB6wimoA</recordid><startdate>201604</startdate><enddate>201604</enddate><creator>Akyol, Çağrı</creator><creator>Turker, Gokhan</creator><creator>Ince, Orhan</creator><creator>Ertekin, Emine</creator><creator>Üstüner, Oya</creator><creator>Ince, Bahar</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7ST</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>SOI</scope></search><sort><creationdate>201604</creationdate><title>Performance and microbial community variations in thermophilic anaerobic digesters treating OTC medicated cow manure under different operational conditions</title><author>Akyol, Çağrı ; 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This study aimed to determine the fate and effect of oxytetracycline (OTC) and its metabolites during thermophilic anaerobic digestion of cow manure. OTC-medicated and non-medicated digesters were operated at 55°C with different volatile solids (VS) concentrations (4% and 6%) and mixing rates (90 and 120rpm). OTC and its metabolites were measured by HPLC and LC/MS/MS, respectively. Microbial community dynamics were monitored by denaturing gradient gel electrophoresis (DGGE) and real-time PCR (qPCR). Approximately 2mg/L initial OTC concentration caused 10–30% inhibition on biogas production and higher inhibition was observed as mixing rate increased. DGGE results indicated that OTC caused a shift in bacterial community structure and several species became dominant with time. Archaeal community decreased throughout the digestion period. RNA based qPCR analyses showed that gene copy numbers of bacteria and Methanomicrobiales declined in all digesters whereas gene copy numbers of Methanobacteriales and Methanosarcinales increased in high mixing rate digesters.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>26826959</pmid><doi>10.1016/j.biortech.2016.01.071</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0960-8524 |
| ispartof | Bioresource technology, 2016-04, Vol.205, p.191-198 |
| issn | 0960-8524 1873-2976 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Anaerobiosis Animals Anti-Bacterial Agents - analysis Anti-Bacterial Agents - metabolism Anti-Bacterial Agents - pharmacology Archaea - drug effects Archaea - genetics Archaea - physiology Biofuels Bioreactors - microbiology Carbon Dioxide - metabolism Cattle Chromatography, High Pressure Liquid Cow manure Denaturing Gradient Gel Electrophoresis DGGE Female Half-Life Hydrogen - metabolism Manure - microbiology Methanomicrobiales Methanomicrobiales - genetics Methanomicrobiales - physiology Methanosarcinales Methanosarcinales - drug effects Methanosarcinales - metabolism Methanosarcinales - physiology Microbial Consortia - drug effects Microbial Consortia - genetics Microbial Consortia - physiology Oxytetracycline Oxytetracycline - analysis Oxytetracycline - metabolism Oxytetracycline - pharmacology Real-time PCR Real-Time Polymerase Chain Reaction Tandem Mass Spectrometry Thermophilic anaerobic digestion |
| title | Performance and microbial community variations in thermophilic anaerobic digesters treating OTC medicated cow manure under different operational conditions |
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