Production of 1,3-propanediol by Clostridium beijerinckii DSM 791 from crude glycerol and corn steep liquor: Process optimization and metabolic engineering

•C. beijerinckii DSM 791 is a robust organism for 1,3-PDO production from glycerol.•Replacing yeast extract with corn steep liquor increased cell growth and productivity.•Stable, long-term 1,3-PDO production was obtained in repeated batch fermentations.•Cell was engineered to overexpress GDH and DHA...

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Veröffentlicht in:	Bioresource technology 2016-07, Vol.212, p.100-110
Hauptverfasser:	Wischral, Daiana, Zhang, Jianzhi, Cheng, Chi, Lin, Meng, De Souza, Lucas Monteiro Galotti, Pessoa, Fernando L. Pellegrini, Pereira, Nei, Yang, Shang-Tian
Format:	Artikel
Sprache:	eng
Schlagworte:	1,3-Propanediol Acetates - metabolism Batch Cell Culture Techniques Bioreactors - microbiology Butyrates - metabolism Clostridium beijerinckii Clostridium beijerinckii - metabolism Clostridium beijerinckii DSM 791 Dihydroxyacetone kinase Escherichia coli Fermentation Glycerol Glycerol - metabolism Glycerol dehydrogenase Hydrogen-Ion Concentration Kinetics Metabolic Engineering - methods Metabolic Networks and Pathways Plasmids - metabolism Propylene Glycols - metabolism Sugar Alcohol Dehydrogenases - metabolism Waste Disposal, Fluid Zea mays - chemistry
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