Heat stress enhances LTM formation in Lymnaea: role of HSPs and DNA methylation
Environmentally relevant stressors alter the memory-forming process in Lymnaea following operant conditioning of aerial respiration. One such stressor is heat. Previously, we found that following a 1 h heat shock, long-term memory (LTM) formation was enhanced. We also had shown that the heat stresso...
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| Veröffentlicht in: | Journal of experimental biology 2016-05, Vol.219 (Pt 9), p.1337-1345 |
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| container_issue | Pt 9 |
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| container_title | Journal of experimental biology |
| container_volume | 219 |
| creator | Sunada, Hiroshi Riaz, Hamza de Freitas, Emily Lukowiak, Kai Swinton, Cayley Swinton, Erin Protheroe, Amy Shymansky, Tamila Komatsuzaki, Yoshimasa Lukowiak, Ken |
| description | Environmentally relevant stressors alter the memory-forming process in Lymnaea following operant conditioning of aerial respiration. One such stressor is heat. Previously, we found that following a 1 h heat shock, long-term memory (LTM) formation was enhanced. We also had shown that the heat stressor activates at least two heat shock proteins (HSPs): HSP40 and HSP70. Here, we tested two hypotheses: (1) the production of HSPs is necessary for enhanced LTM formation; and (2) blocking DNA methylation prevents the heat stressor-induced enhancement of LTM formation. We show here that the enhancing effect of the heat stressor on LTM formation occurs even if snails experienced the stressor 3 days previously. We further show that a flavonoid, quercetin, which inhibits HSP activation, blocks the enhancing effect of the heat stressor on LTM formation. Finally, we show that injection of a DNA methylation blocker, 5-AZA, before snails experience the heat stressor prevents enhancement of memory formation. |
| doi_str_mv | 10.1242/jeb.134296 |
| format | Article |
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One such stressor is heat. Previously, we found that following a 1 h heat shock, long-term memory (LTM) formation was enhanced. We also had shown that the heat stressor activates at least two heat shock proteins (HSPs): HSP40 and HSP70. Here, we tested two hypotheses: (1) the production of HSPs is necessary for enhanced LTM formation; and (2) blocking DNA methylation prevents the heat stressor-induced enhancement of LTM formation. We show here that the enhancing effect of the heat stressor on LTM formation occurs even if snails experienced the stressor 3 days previously. We further show that a flavonoid, quercetin, which inhibits HSP activation, blocks the enhancing effect of the heat stressor on LTM formation. Finally, we show that injection of a DNA methylation blocker, 5-AZA, before snails experience the heat stressor prevents enhancement of memory formation.</description><identifier>ISSN: 0022-0949</identifier><identifier>EISSN: 1477-9145</identifier><identifier>DOI: 10.1242/jeb.134296</identifier><identifier>PMID: 27208033</identifier><language>eng</language><publisher>England</publisher><subject>Animals ; Behavior, Animal ; Conditioning, Operant ; DNA Methylation ; Heat-Shock Proteins - metabolism ; Heat-Shock Response ; Hot Temperature ; HSP40 Heat-Shock Proteins - metabolism ; HSP70 Heat-Shock Proteins - metabolism ; Lymnaea - genetics ; Lymnaea - physiology ; Memory, Long-Term</subject><ispartof>Journal of experimental biology, 2016-05, Vol.219 (Pt 9), p.1337-1345</ispartof><rights>2016. 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One such stressor is heat. Previously, we found that following a 1 h heat shock, long-term memory (LTM) formation was enhanced. We also had shown that the heat stressor activates at least two heat shock proteins (HSPs): HSP40 and HSP70. Here, we tested two hypotheses: (1) the production of HSPs is necessary for enhanced LTM formation; and (2) blocking DNA methylation prevents the heat stressor-induced enhancement of LTM formation. We show here that the enhancing effect of the heat stressor on LTM formation occurs even if snails experienced the stressor 3 days previously. We further show that a flavonoid, quercetin, which inhibits HSP activation, blocks the enhancing effect of the heat stressor on LTM formation. Finally, we show that injection of a DNA methylation blocker, 5-AZA, before snails experience the heat stressor prevents enhancement of memory formation.</description><subject>Animals</subject><subject>Behavior, Animal</subject><subject>Conditioning, Operant</subject><subject>DNA Methylation</subject><subject>Heat-Shock Proteins - metabolism</subject><subject>Heat-Shock Response</subject><subject>Hot Temperature</subject><subject>HSP40 Heat-Shock Proteins - metabolism</subject><subject>HSP70 Heat-Shock Proteins - metabolism</subject><subject>Lymnaea - genetics</subject><subject>Lymnaea - physiology</subject><subject>Memory, Long-Term</subject><issn>0022-0949</issn><issn>1477-9145</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kM1OwkAYRSdGI4hufAAzS2NSnL-2jDsCKiZVTMR1883M11DSH5wpC97eKujd3M3Jzc0h5JqzMRdK3G_QjLlUQicnZMhVmkaaq_iUDBkTImJa6QG5CGHD-iSxOicDkQo2YVIOyXKB0NHQeQyBYrOGxmKg2eqVFq2voSvbhpYNzfZ1AwgP1LcV0ragi4_3QKFxdP42pTV26331C1-SswKqgFfHHpHPp8fVbBFly-eX2TSLrIzTLnJSamMFQ8PEBASYhBVYWKOM07Z_hqlDQFlwixALl0ykVdYloCHmEg2XI3J72N369muHocvrMlisKmiw3YWcp5ppESc87tG7A2p9G4LHIt_6sga_zznLfwTmvcD8ILCHb467O1Oj-0f_jMlvpxhrZg</recordid><startdate>20160501</startdate><enddate>20160501</enddate><creator>Sunada, Hiroshi</creator><creator>Riaz, Hamza</creator><creator>de Freitas, Emily</creator><creator>Lukowiak, Kai</creator><creator>Swinton, Cayley</creator><creator>Swinton, Erin</creator><creator>Protheroe, Amy</creator><creator>Shymansky, Tamila</creator><creator>Komatsuzaki, Yoshimasa</creator><creator>Lukowiak, Ken</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-9028-1931</orcidid></search><sort><creationdate>20160501</creationdate><title>Heat stress enhances LTM formation in Lymnaea: role of HSPs and DNA methylation</title><author>Sunada, Hiroshi ; Riaz, Hamza ; de Freitas, Emily ; Lukowiak, Kai ; Swinton, Cayley ; Swinton, Erin ; Protheroe, Amy ; Shymansky, Tamila ; Komatsuzaki, Yoshimasa ; Lukowiak, Ken</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-d339bc20eb028a2ab60fefcb4bd9c803e7deae3f1cea52d683c4cd6a9a513eb13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Behavior, Animal</topic><topic>Conditioning, Operant</topic><topic>DNA Methylation</topic><topic>Heat-Shock Proteins - metabolism</topic><topic>Heat-Shock Response</topic><topic>Hot Temperature</topic><topic>HSP40 Heat-Shock Proteins - metabolism</topic><topic>HSP70 Heat-Shock Proteins - metabolism</topic><topic>Lymnaea - genetics</topic><topic>Lymnaea - physiology</topic><topic>Memory, Long-Term</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sunada, Hiroshi</creatorcontrib><creatorcontrib>Riaz, Hamza</creatorcontrib><creatorcontrib>de Freitas, Emily</creatorcontrib><creatorcontrib>Lukowiak, Kai</creatorcontrib><creatorcontrib>Swinton, Cayley</creatorcontrib><creatorcontrib>Swinton, Erin</creatorcontrib><creatorcontrib>Protheroe, Amy</creatorcontrib><creatorcontrib>Shymansky, Tamila</creatorcontrib><creatorcontrib>Komatsuzaki, Yoshimasa</creatorcontrib><creatorcontrib>Lukowiak, Ken</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of experimental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sunada, Hiroshi</au><au>Riaz, Hamza</au><au>de Freitas, Emily</au><au>Lukowiak, Kai</au><au>Swinton, Cayley</au><au>Swinton, Erin</au><au>Protheroe, Amy</au><au>Shymansky, Tamila</au><au>Komatsuzaki, Yoshimasa</au><au>Lukowiak, Ken</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Heat stress enhances LTM formation in Lymnaea: role of HSPs and DNA methylation</atitle><jtitle>Journal of experimental biology</jtitle><addtitle>J Exp Biol</addtitle><date>2016-05-01</date><risdate>2016</risdate><volume>219</volume><issue>Pt 9</issue><spage>1337</spage><epage>1345</epage><pages>1337-1345</pages><issn>0022-0949</issn><eissn>1477-9145</eissn><abstract>Environmentally relevant stressors alter the memory-forming process in Lymnaea following operant conditioning of aerial respiration. One such stressor is heat. Previously, we found that following a 1 h heat shock, long-term memory (LTM) formation was enhanced. We also had shown that the heat stressor activates at least two heat shock proteins (HSPs): HSP40 and HSP70. Here, we tested two hypotheses: (1) the production of HSPs is necessary for enhanced LTM formation; and (2) blocking DNA methylation prevents the heat stressor-induced enhancement of LTM formation. We show here that the enhancing effect of the heat stressor on LTM formation occurs even if snails experienced the stressor 3 days previously. We further show that a flavonoid, quercetin, which inhibits HSP activation, blocks the enhancing effect of the heat stressor on LTM formation. Finally, we show that injection of a DNA methylation blocker, 5-AZA, before snails experience the heat stressor prevents enhancement of memory formation.</abstract><cop>England</cop><pmid>27208033</pmid><doi>10.1242/jeb.134296</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-9028-1931</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection; Company of Biologists |
| subjects | Animals Behavior, Animal Conditioning, Operant DNA Methylation Heat-Shock Proteins - metabolism Heat-Shock Response Hot Temperature HSP40 Heat-Shock Proteins - metabolism HSP70 Heat-Shock Proteins - metabolism Lymnaea - genetics Lymnaea - physiology Memory, Long-Term |
| title | Heat stress enhances LTM formation in Lymnaea: role of HSPs and DNA methylation |
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