The effects of diazinon on lipid peroxidation and antioxidant enzymes in erythrocytes in vitro
Diazinon is one of the most widely used organophosphate insecticides (OPI) in agriculture and public health programs. The aim of this study was to investigate how an OPI, diazinon, affects lipid peroxidation (LPO) and the antioxidant defense system in vitro. For this purpose, two experiments were ca...
Gespeichert in:
| Veröffentlicht in: | Human & experimental toxicology 2004-01, Vol.23 (1), p.9-13 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 13 |
|---|---|
| container_issue | 1 |
| container_start_page | 9 |
| container_title | Human & experimental toxicology |
| container_volume | 23 |
| creator | Altuntas, Irfan Kilinc, Ibrahim Orhan, Hikmet Demirel, Reha Koylu, Halis Delibas, Namik |
| description | Diazinon is one of the most widely used organophosphate insecticides (OPI) in agriculture and public health programs. The aim of this study was to investigate how an OPI, diazinon, affects lipid peroxidation (LPO) and the antioxidant defense system in vitro. For this purpose, two experiments were carried out. In experiment 1, the effects of various concentrations of diazinon on LPO and the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) in erythrocytes were studied. Each diazinon concentration was incubated with a previously prepared erythrocyte samples at +4°C for 0, 60 and 180 min. After incubation, the malondialdehyde (MDA) levels and the activities of SOD, GSH-Px and CAT were determined. In experiment 2, in order to determine the direct effect of diazinon on the activities of SOD, GSH-Px and CAT, the erythrocytes were haemolysed and incubated with the various concentrations of diazinon at +4°C for 0, 60 and 180 min. In experiment 1, MDA levels and the activities of SOD and GSH-Px increased with increasing diazinon concentration and incubation period, but CAT activity remained unchanged. In experiment 2, SOD activity was significantly decreased, and GSH-Px activity was significantly increased. From these results, it can be concluded that in vitro administration of diazinon results in the induction of erythrocyte LPO and changes the activities of antioxidant enzymes, suggesting that reactive oxygen species may be involved in the toxic effects of diazinon. |
| doi_str_mv | 10.1191/0960327104ht408oa |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_AFRWT</sourceid><recordid>TN_cdi_proquest_miscellaneous_17908094</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sage_id>10.1191_0960327104ht408oa</sage_id><sourcerecordid>791288731</sourcerecordid><originalsourceid>FETCH-LOGICAL-c422t-99adf7c42637475a1735470621e38adf2bb7f360288e4bf884f09a879478945d3</originalsourceid><addsrcrecordid>eNp1kMtKAzEUhoMotlYfwI0Mgu6mnmQyk2QpxRsU3NStQzqT2JRpUpNUbJ_elBYKipBDzuU7F36ELjEMMRb4DkQFBWEY6CxS4E4eoT6mjOUgoDhG_W093wI9dBbCHAAqUeJT1MMlEMYx9NH7ZKYypbVqYsiczlojN8Y6m6XXmaVps6Xy7tu0MpqUkrZNltxtxsZM2c16oUJmbKb8Os68a9ZxF3-Z6N05OtGyC-pi_w_Q2-PDZPScj1-fXkb347yhhMRcCNlqlvyqYJSVErOipAwqglXBU4lMp0wXFRDOFZ1qzqkGITkTlHFBy7YYoNvd3KV3nysVYr0woVFdJ61yq1BjJoCDoAm8_gXO3crbdFtNCHBc4iTdAOEd1HgXgle6XnqzkH5dY6i3ytd_lE89V_vBq-lCtYeOvdQJuNkDMjSy017axoQDVwElJeeJG-64ID_U4br_N_8ATsyZow</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>220815190</pqid></control><display><type>article</type><title>The effects of diazinon on lipid peroxidation and antioxidant enzymes in erythrocytes in vitro</title><source>Sage Journals GOLD Open Access 2024</source><creator>Altuntas, Irfan ; Kilinc, Ibrahim ; Orhan, Hikmet ; Demirel, Reha ; Koylu, Halis ; Delibas, Namik</creator><creatorcontrib>Altuntas, Irfan ; Kilinc, Ibrahim ; Orhan, Hikmet ; Demirel, Reha ; Koylu, Halis ; Delibas, Namik</creatorcontrib><description>Diazinon is one of the most widely used organophosphate insecticides (OPI) in agriculture and public health programs. The aim of this study was to investigate how an OPI, diazinon, affects lipid peroxidation (LPO) and the antioxidant defense system in vitro. For this purpose, two experiments were carried out. In experiment 1, the effects of various concentrations of diazinon on LPO and the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) in erythrocytes were studied. Each diazinon concentration was incubated with a previously prepared erythrocyte samples at +4°C for 0, 60 and 180 min. After incubation, the malondialdehyde (MDA) levels and the activities of SOD, GSH-Px and CAT were determined. In experiment 2, in order to determine the direct effect of diazinon on the activities of SOD, GSH-Px and CAT, the erythrocytes were haemolysed and incubated with the various concentrations of diazinon at +4°C for 0, 60 and 180 min. In experiment 1, MDA levels and the activities of SOD and GSH-Px increased with increasing diazinon concentration and incubation period, but CAT activity remained unchanged. In experiment 2, SOD activity was significantly decreased, and GSH-Px activity was significantly increased. From these results, it can be concluded that in vitro administration of diazinon results in the induction of erythrocyte LPO and changes the activities of antioxidant enzymes, suggesting that reactive oxygen species may be involved in the toxic effects of diazinon.</description><identifier>ISSN: 0960-3271</identifier><identifier>EISSN: 1477-0903</identifier><identifier>DOI: 10.1191/0960327104ht408oa</identifier><identifier>PMID: 15027810</identifier><language>eng</language><publisher>Thousand Oaks, CA: SAGE Publications</publisher><subject>Adult ; Agricultural production ; Agriculture ; Analysis of Variance ; Antioxidants ; Biochemistry ; Biological and medical sciences ; Catalase - drug effects ; Catalase - metabolism ; Cells, Cultured ; Developing countries ; Diazinon - pharmacology ; Enzymes ; Erythrocytes - drug effects ; Erythrocytes - enzymology ; Experiments ; Glutathione Peroxidase - drug effects ; Glutathione Peroxidase - metabolism ; Humans ; Insecticides ; Insecticides - pharmacology ; LDCs ; Lipid peroxidation ; Lipid Peroxidation - drug effects ; Lipids ; Male ; Medical research ; Medical sciences ; Medicine ; Pesticides, fertilizers and other agrochemicals toxicology ; Poisoning ; Public health ; Superoxide Dismutase - drug effects ; Superoxide Dismutase - metabolism ; Toxicity ; Toxicology</subject><ispartof>Human & experimental toxicology, 2004-01, Vol.23 (1), p.9-13</ispartof><rights>2004 INIST-CNRS</rights><rights>2004 Arnold</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-99adf7c42637475a1735470621e38adf2bb7f360288e4bf884f09a879478945d3</citedby><cites>FETCH-LOGICAL-c422t-99adf7c42637475a1735470621e38adf2bb7f360288e4bf884f09a879478945d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1191/0960327104ht408oa$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1191/0960327104ht408oa$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,776,780,4010,21945,27830,27900,27901,27902,44921,45309</link.rule.ids><linktorsrc>$$Uhttps://journals.sagepub.com/doi/full/10.1191/0960327104ht408oa?utm_source=summon&utm_medium=discovery-provider$$EView_record_in_SAGE_Publications$$FView_record_in_$$GSAGE_Publications</linktorsrc><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16042588$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15027810$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Altuntas, Irfan</creatorcontrib><creatorcontrib>Kilinc, Ibrahim</creatorcontrib><creatorcontrib>Orhan, Hikmet</creatorcontrib><creatorcontrib>Demirel, Reha</creatorcontrib><creatorcontrib>Koylu, Halis</creatorcontrib><creatorcontrib>Delibas, Namik</creatorcontrib><title>The effects of diazinon on lipid peroxidation and antioxidant enzymes in erythrocytes in vitro</title><title>Human & experimental toxicology</title><addtitle>Hum Exp Toxicol</addtitle><description>Diazinon is one of the most widely used organophosphate insecticides (OPI) in agriculture and public health programs. The aim of this study was to investigate how an OPI, diazinon, affects lipid peroxidation (LPO) and the antioxidant defense system in vitro. For this purpose, two experiments were carried out. In experiment 1, the effects of various concentrations of diazinon on LPO and the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) in erythrocytes were studied. Each diazinon concentration was incubated with a previously prepared erythrocyte samples at +4°C for 0, 60 and 180 min. After incubation, the malondialdehyde (MDA) levels and the activities of SOD, GSH-Px and CAT were determined. In experiment 2, in order to determine the direct effect of diazinon on the activities of SOD, GSH-Px and CAT, the erythrocytes were haemolysed and incubated with the various concentrations of diazinon at +4°C for 0, 60 and 180 min. In experiment 1, MDA levels and the activities of SOD and GSH-Px increased with increasing diazinon concentration and incubation period, but CAT activity remained unchanged. In experiment 2, SOD activity was significantly decreased, and GSH-Px activity was significantly increased. From these results, it can be concluded that in vitro administration of diazinon results in the induction of erythrocyte LPO and changes the activities of antioxidant enzymes, suggesting that reactive oxygen species may be involved in the toxic effects of diazinon.</description><subject>Adult</subject><subject>Agricultural production</subject><subject>Agriculture</subject><subject>Analysis of Variance</subject><subject>Antioxidants</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Catalase - drug effects</subject><subject>Catalase - metabolism</subject><subject>Cells, Cultured</subject><subject>Developing countries</subject><subject>Diazinon - pharmacology</subject><subject>Enzymes</subject><subject>Erythrocytes - drug effects</subject><subject>Erythrocytes - enzymology</subject><subject>Experiments</subject><subject>Glutathione Peroxidase - drug effects</subject><subject>Glutathione Peroxidase - metabolism</subject><subject>Humans</subject><subject>Insecticides</subject><subject>Insecticides - pharmacology</subject><subject>LDCs</subject><subject>Lipid peroxidation</subject><subject>Lipid Peroxidation - drug effects</subject><subject>Lipids</subject><subject>Male</subject><subject>Medical research</subject><subject>Medical sciences</subject><subject>Medicine</subject><subject>Pesticides, fertilizers and other agrochemicals toxicology</subject><subject>Poisoning</subject><subject>Public health</subject><subject>Superoxide Dismutase - drug effects</subject><subject>Superoxide Dismutase - metabolism</subject><subject>Toxicity</subject><subject>Toxicology</subject><issn>0960-3271</issn><issn>1477-0903</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kMtKAzEUhoMotlYfwI0Mgu6mnmQyk2QpxRsU3NStQzqT2JRpUpNUbJ_elBYKipBDzuU7F36ELjEMMRb4DkQFBWEY6CxS4E4eoT6mjOUgoDhG_W093wI9dBbCHAAqUeJT1MMlEMYx9NH7ZKYypbVqYsiczlojN8Y6m6XXmaVps6Xy7tu0MpqUkrZNltxtxsZM2c16oUJmbKb8Os68a9ZxF3-Z6N05OtGyC-pi_w_Q2-PDZPScj1-fXkb347yhhMRcCNlqlvyqYJSVErOipAwqglXBU4lMp0wXFRDOFZ1qzqkGITkTlHFBy7YYoNvd3KV3nysVYr0woVFdJ61yq1BjJoCDoAm8_gXO3crbdFtNCHBc4iTdAOEd1HgXgle6XnqzkH5dY6i3ytd_lE89V_vBq-lCtYeOvdQJuNkDMjSy017axoQDVwElJeeJG-64ID_U4br_N_8ATsyZow</recordid><startdate>200401</startdate><enddate>200401</enddate><creator>Altuntas, Irfan</creator><creator>Kilinc, Ibrahim</creator><creator>Orhan, Hikmet</creator><creator>Demirel, Reha</creator><creator>Koylu, Halis</creator><creator>Delibas, Namik</creator><general>SAGE Publications</general><general>Arnold</general><general>Sage Publications Ltd</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7ST</scope><scope>7TK</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>SOI</scope></search><sort><creationdate>200401</creationdate><title>The effects of diazinon on lipid peroxidation and antioxidant enzymes in erythrocytes in vitro</title><author>Altuntas, Irfan ; Kilinc, Ibrahim ; Orhan, Hikmet ; Demirel, Reha ; Koylu, Halis ; Delibas, Namik</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-99adf7c42637475a1735470621e38adf2bb7f360288e4bf884f09a879478945d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adult</topic><topic>Agricultural production</topic><topic>Agriculture</topic><topic>Analysis of Variance</topic><topic>Antioxidants</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Catalase - drug effects</topic><topic>Catalase - metabolism</topic><topic>Cells, Cultured</topic><topic>Developing countries</topic><topic>Diazinon - pharmacology</topic><topic>Enzymes</topic><topic>Erythrocytes - drug effects</topic><topic>Erythrocytes - enzymology</topic><topic>Experiments</topic><topic>Glutathione Peroxidase - drug effects</topic><topic>Glutathione Peroxidase - metabolism</topic><topic>Humans</topic><topic>Insecticides</topic><topic>Insecticides - pharmacology</topic><topic>LDCs</topic><topic>Lipid peroxidation</topic><topic>Lipid Peroxidation - drug effects</topic><topic>Lipids</topic><topic>Male</topic><topic>Medical research</topic><topic>Medical sciences</topic><topic>Medicine</topic><topic>Pesticides, fertilizers and other agrochemicals toxicology</topic><topic>Poisoning</topic><topic>Public health</topic><topic>Superoxide Dismutase - drug effects</topic><topic>Superoxide Dismutase - metabolism</topic><topic>Toxicity</topic><topic>Toxicology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Altuntas, Irfan</creatorcontrib><creatorcontrib>Kilinc, Ibrahim</creatorcontrib><creatorcontrib>Orhan, Hikmet</creatorcontrib><creatorcontrib>Demirel, Reha</creatorcontrib><creatorcontrib>Koylu, Halis</creatorcontrib><creatorcontrib>Delibas, Namik</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Environment Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Environment Abstracts</collection><jtitle>Human & experimental toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Altuntas, Irfan</au><au>Kilinc, Ibrahim</au><au>Orhan, Hikmet</au><au>Demirel, Reha</au><au>Koylu, Halis</au><au>Delibas, Namik</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effects of diazinon on lipid peroxidation and antioxidant enzymes in erythrocytes in vitro</atitle><jtitle>Human & experimental toxicology</jtitle><addtitle>Hum Exp Toxicol</addtitle><date>2004-01</date><risdate>2004</risdate><volume>23</volume><issue>1</issue><spage>9</spage><epage>13</epage><pages>9-13</pages><issn>0960-3271</issn><eissn>1477-0903</eissn><abstract>Diazinon is one of the most widely used organophosphate insecticides (OPI) in agriculture and public health programs. The aim of this study was to investigate how an OPI, diazinon, affects lipid peroxidation (LPO) and the antioxidant defense system in vitro. For this purpose, two experiments were carried out. In experiment 1, the effects of various concentrations of diazinon on LPO and the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) in erythrocytes were studied. Each diazinon concentration was incubated with a previously prepared erythrocyte samples at +4°C for 0, 60 and 180 min. After incubation, the malondialdehyde (MDA) levels and the activities of SOD, GSH-Px and CAT were determined. In experiment 2, in order to determine the direct effect of diazinon on the activities of SOD, GSH-Px and CAT, the erythrocytes were haemolysed and incubated with the various concentrations of diazinon at +4°C for 0, 60 and 180 min. In experiment 1, MDA levels and the activities of SOD and GSH-Px increased with increasing diazinon concentration and incubation period, but CAT activity remained unchanged. In experiment 2, SOD activity was significantly decreased, and GSH-Px activity was significantly increased. From these results, it can be concluded that in vitro administration of diazinon results in the induction of erythrocyte LPO and changes the activities of antioxidant enzymes, suggesting that reactive oxygen species may be involved in the toxic effects of diazinon.</abstract><cop>Thousand Oaks, CA</cop><pub>SAGE Publications</pub><pmid>15027810</pmid><doi>10.1191/0960327104ht408oa</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | ISSN: 0960-3271 |
| ispartof | Human & experimental toxicology, 2004-01, Vol.23 (1), p.9-13 |
| issn | 0960-3271 1477-0903 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_17908094 |
| source | Sage Journals GOLD Open Access 2024 |
| subjects | Adult Agricultural production Agriculture Analysis of Variance Antioxidants Biochemistry Biological and medical sciences Catalase - drug effects Catalase - metabolism Cells, Cultured Developing countries Diazinon - pharmacology Enzymes Erythrocytes - drug effects Erythrocytes - enzymology Experiments Glutathione Peroxidase - drug effects Glutathione Peroxidase - metabolism Humans Insecticides Insecticides - pharmacology LDCs Lipid peroxidation Lipid Peroxidation - drug effects Lipids Male Medical research Medical sciences Medicine Pesticides, fertilizers and other agrochemicals toxicology Poisoning Public health Superoxide Dismutase - drug effects Superoxide Dismutase - metabolism Toxicity Toxicology |
| title | The effects of diazinon on lipid peroxidation and antioxidant enzymes in erythrocytes in vitro |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-15T11%3A00%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_AFRWT&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20effects%20of%20diazinon%20on%20lipid%20peroxidation%20and%20antioxidant%20enzymes%20in%20erythrocytes%20in%20vitro&rft.jtitle=Human%20&%20experimental%20toxicology&rft.au=Altuntas,%20Irfan&rft.date=2004-01&rft.volume=23&rft.issue=1&rft.spage=9&rft.epage=13&rft.pages=9-13&rft.issn=0960-3271&rft.eissn=1477-0903&rft_id=info:doi/10.1191/0960327104ht408oa&rft_dat=%3Cproquest_AFRWT%3E791288731%3C/proquest_AFRWT%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=220815190&rft_id=info:pmid/15027810&rft_sage_id=10.1191_0960327104ht408oa&rfr_iscdi=true |