CCC1 Is a Transporter That Mediates Vacuolar Iron Storage in Yeast
The budding yeast Saccharomyces cerevisiae can grow for generations in the absence of exogenous iron, indicating a capacity to store intracellular iron. As cells can accumulate iron by endocytosis we studied iron metabolism in yeast that were defective in endocytosis. We demonstrated that endocytosi...
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| Veröffentlicht in: | The Journal of biological chemistry 2001-08, Vol.276 (31), p.29515-29519 |
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| container_title | The Journal of biological chemistry |
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| creator | Li, L Chen, O S McVey Ward, D Kaplan, J |
| description | The budding yeast Saccharomyces cerevisiae can grow for generations in the absence of exogenous iron, indicating a capacity to store intracellular iron. As cells can
accumulate iron by endocytosis we studied iron metabolism in yeast that were defective in endocytosis. We demonstrated that
endocytosis-defective yeast ( Îend4 ) can store iron in the vacuole, indicating a transfer of iron from the cytosol to the vacuole. Using several different criteria
we demonstrated that CCC1 encodes a transporter that effects the accumulation of iron and Mn 2+ in vacuoles. Overexpression of CCC1, which is localized to the vacuole , lowers cytosolic iron and increases vacuolar iron content. Conversely, deletion of CCC1 results in decreased vacuolar iron content and decreased iron stores, which affect cytosolic iron levels and cell growth.
Furthermore Îccc1 cells show increased sensitivity to external iron. The sensitivity to iron is exacerbated by ectopic expression of the iron
transporter FET4 . These results indicate that yeast can store iron in the vacuole and that CCC1 is involved in the transfer of iron from the cytosol to the vacuole. |
| doi_str_mv | 10.1074/jbc.M103944200 |
| format | Article |
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accumulate iron by endocytosis we studied iron metabolism in yeast that were defective in endocytosis. We demonstrated that
endocytosis-defective yeast ( Îend4 ) can store iron in the vacuole, indicating a transfer of iron from the cytosol to the vacuole. Using several different criteria
we demonstrated that CCC1 encodes a transporter that effects the accumulation of iron and Mn 2+ in vacuoles. Overexpression of CCC1, which is localized to the vacuole , lowers cytosolic iron and increases vacuolar iron content. Conversely, deletion of CCC1 results in decreased vacuolar iron content and decreased iron stores, which affect cytosolic iron levels and cell growth.
Furthermore Îccc1 cells show increased sensitivity to external iron. The sensitivity to iron is exacerbated by ectopic expression of the iron
transporter FET4 . These results indicate that yeast can store iron in the vacuole and that CCC1 is involved in the transfer of iron from the cytosol to the vacuole.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1074/jbc.M103944200</identifier><identifier>PMID: 11390404</identifier><language>eng</language><publisher>United States: American Society for Biochemistry and Molecular Biology</publisher><subject>beta-Galactosidase - metabolism ; Cation Transport Proteins ; CCC1 gene ; Cell Division ; Ceruloplasmin - genetics ; Ceruloplasmin - metabolism ; Cytosol - metabolism ; DNA Primers ; end4 gene ; FET4 gene ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; Gene Deletion ; Genes, Reporter ; Iron - metabolism ; Kinetics ; Manganese - metabolism ; Recombinant Fusion Proteins - metabolism ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - growth & development ; Saccharomyces cerevisiae - metabolism ; Saccharomyces cerevisiae Proteins ; transporters ; Vacuoles - metabolism</subject><ispartof>The Journal of biological chemistry, 2001-08, Vol.276 (31), p.29515-29519</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-6a1c544cc9c1b6e3e3d80a1f00121ddbe71f6e4e2dd882f7416c0d7e73172fdf3</citedby><cites>FETCH-LOGICAL-c391t-6a1c544cc9c1b6e3e3d80a1f00121ddbe71f6e4e2dd882f7416c0d7e73172fdf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27907,27908</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11390404$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, L</creatorcontrib><creatorcontrib>Chen, O S</creatorcontrib><creatorcontrib>McVey Ward, D</creatorcontrib><creatorcontrib>Kaplan, J</creatorcontrib><title>CCC1 Is a Transporter That Mediates Vacuolar Iron Storage in Yeast</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>The budding yeast Saccharomyces cerevisiae can grow for generations in the absence of exogenous iron, indicating a capacity to store intracellular iron. As cells can
accumulate iron by endocytosis we studied iron metabolism in yeast that were defective in endocytosis. We demonstrated that
endocytosis-defective yeast ( Îend4 ) can store iron in the vacuole, indicating a transfer of iron from the cytosol to the vacuole. Using several different criteria
we demonstrated that CCC1 encodes a transporter that effects the accumulation of iron and Mn 2+ in vacuoles. Overexpression of CCC1, which is localized to the vacuole , lowers cytosolic iron and increases vacuolar iron content. Conversely, deletion of CCC1 results in decreased vacuolar iron content and decreased iron stores, which affect cytosolic iron levels and cell growth.
Furthermore Îccc1 cells show increased sensitivity to external iron. The sensitivity to iron is exacerbated by ectopic expression of the iron
transporter FET4 . These results indicate that yeast can store iron in the vacuole and that CCC1 is involved in the transfer of iron from the cytosol to the vacuole.</description><subject>beta-Galactosidase - metabolism</subject><subject>Cation Transport Proteins</subject><subject>CCC1 gene</subject><subject>Cell Division</subject><subject>Ceruloplasmin - genetics</subject><subject>Ceruloplasmin - metabolism</subject><subject>Cytosol - metabolism</subject><subject>DNA Primers</subject><subject>end4 gene</subject><subject>FET4 gene</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>Gene Deletion</subject><subject>Genes, Reporter</subject><subject>Iron - metabolism</subject><subject>Kinetics</subject><subject>Manganese - metabolism</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - growth & development</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Saccharomyces cerevisiae Proteins</subject><subject>transporters</subject><subject>Vacuoles - metabolism</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkDtPwzAUhS0EoqWwMiIPiC3lXtt5jRDxqNSKgYJgshz7pg1qm2InQvx7glqpZznLd87wMXaJMEZI1e1XacczBJkrJQCO2BAhk5GM8eOYDQEERrmIswE7C-EL-qgcT9kAUeagQA3ZfVEUyCeBGz73ZhO2jW_J8_nStHxGrjYtBf5ubNesjOcT32z4a9t4syBeb_gnmdCes5PKrAJd7HvE3h4f5sVzNH15mhR308jKHNsoMWhjpazNLZYJSZIuA4MVAAp0rqQUq4QUCeeyTFSpwsSCSymVmIrKVXLEbna_W998dxRava6DpdXKbKjpgsY0hxiSrAfHO9D6JgRPld76em38r0bQ_9Z0b00frPWDq_1zV67JHfC9ph643gHLerH8qT3psm7sktZapImWqEUeYyz_AFC3cn8</recordid><startdate>20010803</startdate><enddate>20010803</enddate><creator>Li, L</creator><creator>Chen, O S</creator><creator>McVey Ward, D</creator><creator>Kaplan, J</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20010803</creationdate><title>CCC1 Is a Transporter That Mediates Vacuolar Iron Storage in Yeast</title><author>Li, L ; Chen, O S ; McVey Ward, D ; Kaplan, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-6a1c544cc9c1b6e3e3d80a1f00121ddbe71f6e4e2dd882f7416c0d7e73172fdf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>beta-Galactosidase - metabolism</topic><topic>Cation Transport Proteins</topic><topic>CCC1 gene</topic><topic>Cell Division</topic><topic>Ceruloplasmin - genetics</topic><topic>Ceruloplasmin - metabolism</topic><topic>Cytosol - metabolism</topic><topic>DNA Primers</topic><topic>end4 gene</topic><topic>FET4 gene</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - metabolism</topic><topic>Gene Deletion</topic><topic>Genes, Reporter</topic><topic>Iron - metabolism</topic><topic>Kinetics</topic><topic>Manganese - metabolism</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - growth & development</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Saccharomyces cerevisiae Proteins</topic><topic>transporters</topic><topic>Vacuoles - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, L</creatorcontrib><creatorcontrib>Chen, O S</creatorcontrib><creatorcontrib>McVey Ward, D</creatorcontrib><creatorcontrib>Kaplan, J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, L</au><au>Chen, O S</au><au>McVey Ward, D</au><au>Kaplan, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CCC1 Is a Transporter That Mediates Vacuolar Iron Storage in Yeast</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>2001-08-03</date><risdate>2001</risdate><volume>276</volume><issue>31</issue><spage>29515</spage><epage>29519</epage><pages>29515-29519</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>The budding yeast Saccharomyces cerevisiae can grow for generations in the absence of exogenous iron, indicating a capacity to store intracellular iron. As cells can
accumulate iron by endocytosis we studied iron metabolism in yeast that were defective in endocytosis. We demonstrated that
endocytosis-defective yeast ( Îend4 ) can store iron in the vacuole, indicating a transfer of iron from the cytosol to the vacuole. Using several different criteria
we demonstrated that CCC1 encodes a transporter that effects the accumulation of iron and Mn 2+ in vacuoles. Overexpression of CCC1, which is localized to the vacuole , lowers cytosolic iron and increases vacuolar iron content. Conversely, deletion of CCC1 results in decreased vacuolar iron content and decreased iron stores, which affect cytosolic iron levels and cell growth.
Furthermore Îccc1 cells show increased sensitivity to external iron. The sensitivity to iron is exacerbated by ectopic expression of the iron
transporter FET4 . These results indicate that yeast can store iron in the vacuole and that CCC1 is involved in the transfer of iron from the cytosol to the vacuole.</abstract><cop>United States</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>11390404</pmid><doi>10.1074/jbc.M103944200</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | beta-Galactosidase - metabolism Cation Transport Proteins CCC1 gene Cell Division Ceruloplasmin - genetics Ceruloplasmin - metabolism Cytosol - metabolism DNA Primers end4 gene FET4 gene Fungal Proteins - genetics Fungal Proteins - metabolism Gene Deletion Genes, Reporter Iron - metabolism Kinetics Manganese - metabolism Recombinant Fusion Proteins - metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - growth & development Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins transporters Vacuoles - metabolism |
| title | CCC1 Is a Transporter That Mediates Vacuolar Iron Storage in Yeast |
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