Real-time monitoring of basal H2O2 levels with peroxiredoxin-based probes

Editorial Summary A fluorescent sensor combining a mutated form of the 2-Cys peroxiredoxin Tsa2 unable to undergo thioredoxin-mediated reduction with a redox-sensitive GFP protein allows real-time detection of baseline hydrogen peroxide levels in yeast cells. Genetically encoded probes based on the...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Nature chemical biology 2016-06, Vol.12 (6), p.437-443
Hauptverfasser:	Morgan, Bruce, Van Laer, Koen, Owusu, Theresa N E, Ezeriņa, Daria, Pastor-Flores, Daniel, Amponsah, Prince Saforo, Tursch, Anja, Dick, Tobias P
Format:	Artikel
Sprache:	eng
Schlagworte:	14/35 631/1647/245 631/443/319 631/45 631/92/96 Biochemical Engineering Biochemistry Bioorganic Chemistry Cell Biology Cell growth Chemistry Chemistry/Food Science Cytosol - metabolism Homeostasis Hydrogen peroxide Hydrogen Peroxide - analysis Hydrogen Peroxide - metabolism Mitochondria - metabolism Molecular Probes - metabolism Oxygen - metabolism Partial Pressure Peroxiredoxins - metabolism Probes Proteins Saccharomyces cerevisiae - cytology Saccharomyces cerevisiae - growth & development Saccharomyces cerevisiae - metabolism Time Factors Yeasts
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!