Structural elucidation of a pectin from flowers of Lonicera japonica and its antipancreatic cancer activity

[Display omitted] ⿢A new rhamnogalacturonan from flowers of Lonicera japonica is characterized.⿢Backbone is alternate 1,4-α-d-GalpA and 1,2-α-l-Rhap.⿢Branches contain T- and 1,4,6-linked β-d-Galp, T- and 1,5-linked α-l-Araf.⿢Rhamnogalacturonan might inhibit BxPC-3 and PANC-1 pancreatic cancer cells...

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Veröffentlicht in:International journal of biological macromolecules 2016-07, Vol.88, p.130-137
Hauptverfasser: Lin, Liyan, Wang, Peipei, Du, Zhenyun, Wang, Wucheng, Cong, Qifei, Zheng, Changping, Jin, Can, Ding, Kan, Shao, Chenghao
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container_issue
container_start_page 130
container_title International journal of biological macromolecules
container_volume 88
creator Lin, Liyan
Wang, Peipei
Du, Zhenyun
Wang, Wucheng
Cong, Qifei
Zheng, Changping
Jin, Can
Ding, Kan
Shao, Chenghao
description [Display omitted] ⿢A new rhamnogalacturonan from flowers of Lonicera japonica is characterized.⿢Backbone is alternate 1,4-α-d-GalpA and 1,2-α-l-Rhap.⿢Branches contain T- and 1,4,6-linked β-d-Galp, T- and 1,5-linked α-l-Araf.⿢Rhamnogalacturonan might inhibit BxPC-3 and PANC-1 pancreatic cancer cells growth. To investigate polysaccharide structure from Lonicera japonica, and study its effects on behavior of pancreatic cells, a homogenous polysaccharide, LJ-02-1, was extracted and purified from flowers of L. japonica by DEAE-cellulose and Sephacryl S-200HR column. The molecular weight was estimated to be 54kDa. Monosaccharide composition was determined to be rhamnose, galacturonic acid, galactose and arabinose in the molar ratio of 10.77:7.88:15.45:65.89 by analyzing the PMP derivatives of the monosaccharides from 2M trifluoracetic acid hydrolysis via HPLC. Based on methylation analysis, partial acid hydrolysis, and NMR spectra, the polysaccharide was elucidated to be a rhamnogalacturonan backbone and substituted partly at C-4 of rhamnose. The branches were determined to be T- and 1,4,6-linked β-d-Galp, T- and 1,5-linked α-l-Araf. The polysaccharide might inhibit BxPC-3 and PANC-1 pancreatic cancer cells growth at the concentration of 1mg/mL with inhibitory ratio of 66.7% and 52.1%, respectively.
doi_str_mv 10.1016/j.ijbiomac.2016.03.025
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To investigate polysaccharide structure from Lonicera japonica, and study its effects on behavior of pancreatic cells, a homogenous polysaccharide, LJ-02-1, was extracted and purified from flowers of L. japonica by DEAE-cellulose and Sephacryl S-200HR column. The molecular weight was estimated to be 54kDa. Monosaccharide composition was determined to be rhamnose, galacturonic acid, galactose and arabinose in the molar ratio of 10.77:7.88:15.45:65.89 by analyzing the PMP derivatives of the monosaccharides from 2M trifluoracetic acid hydrolysis via HPLC. Based on methylation analysis, partial acid hydrolysis, and NMR spectra, the polysaccharide was elucidated to be a rhamnogalacturonan backbone and substituted partly at C-4 of rhamnose. The branches were determined to be T- and 1,4,6-linked β-d-Galp, T- and 1,5-linked α-l-Araf. The polysaccharide might inhibit BxPC-3 and PANC-1 pancreatic cancer cells growth at the concentration of 1mg/mL with inhibitory ratio of 66.7% and 52.1%, respectively.</description><identifier>ISSN: 0141-8130</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2016.03.025</identifier><identifier>PMID: 27000440</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Antineoplastic Agents, Phytogenic - chemistry ; Antineoplastic Agents, Phytogenic - isolation &amp; purification ; Antineoplastic Agents, Phytogenic - pharmacology ; Antipancreatic cancer cells growth ; Carbohydrate Sequence ; Cell Line, Tumor ; Cell Survival - drug effects ; Dose-Response Relationship, Drug ; Epithelial Cells - drug effects ; Epithelial Cells - pathology ; Flowers - chemistry ; Humans ; Hydrolysis ; Lonicera - chemistry ; Lonicera japonica ; Molecular Weight ; Pancreas - drug effects ; Pancreas - pathology ; Pectins - chemistry ; Pectins - isolation &amp; purification ; Pectins - pharmacology ; Polysaccharide ; Rhamnose - chemistry ; Rhamnose - isolation &amp; purification ; Trifluoroacetic Acid - chemistry</subject><ispartof>International journal of biological macromolecules, 2016-07, Vol.88, p.130-137</ispartof><rights>2016 Elsevier B.V.</rights><rights>Copyright © 2016 Elsevier B.V. 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To investigate polysaccharide structure from Lonicera japonica, and study its effects on behavior of pancreatic cells, a homogenous polysaccharide, LJ-02-1, was extracted and purified from flowers of L. japonica by DEAE-cellulose and Sephacryl S-200HR column. The molecular weight was estimated to be 54kDa. Monosaccharide composition was determined to be rhamnose, galacturonic acid, galactose and arabinose in the molar ratio of 10.77:7.88:15.45:65.89 by analyzing the PMP derivatives of the monosaccharides from 2M trifluoracetic acid hydrolysis via HPLC. Based on methylation analysis, partial acid hydrolysis, and NMR spectra, the polysaccharide was elucidated to be a rhamnogalacturonan backbone and substituted partly at C-4 of rhamnose. The branches were determined to be T- and 1,4,6-linked β-d-Galp, T- and 1,5-linked α-l-Araf. The polysaccharide might inhibit BxPC-3 and PANC-1 pancreatic cancer cells growth at the concentration of 1mg/mL with inhibitory ratio of 66.7% and 52.1%, respectively.</description><subject>Antineoplastic Agents, Phytogenic - chemistry</subject><subject>Antineoplastic Agents, Phytogenic - isolation &amp; purification</subject><subject>Antineoplastic Agents, Phytogenic - pharmacology</subject><subject>Antipancreatic cancer cells growth</subject><subject>Carbohydrate Sequence</subject><subject>Cell Line, Tumor</subject><subject>Cell Survival - drug effects</subject><subject>Dose-Response Relationship, Drug</subject><subject>Epithelial Cells - drug effects</subject><subject>Epithelial Cells - pathology</subject><subject>Flowers - chemistry</subject><subject>Humans</subject><subject>Hydrolysis</subject><subject>Lonicera - chemistry</subject><subject>Lonicera japonica</subject><subject>Molecular Weight</subject><subject>Pancreas - drug effects</subject><subject>Pancreas - pathology</subject><subject>Pectins - chemistry</subject><subject>Pectins - isolation &amp; purification</subject><subject>Pectins - pharmacology</subject><subject>Polysaccharide</subject><subject>Rhamnose - chemistry</subject><subject>Rhamnose - isolation &amp; purification</subject><subject>Trifluoroacetic Acid - chemistry</subject><issn>0141-8130</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE9v3CAQxVHVqtmk_QoRx17sgDGYvbWK-k9aqYe2Z4SHQcK1jQs4Vb59WW3Sa0_zmHnzRvwIueWs5Yyru6kN0xjiYqHt6rtlomWdfEEOXA_HhjEmXpID4z1vNBfsilznPNWukly_JlfdUHXfswP59b2kHcqe7Exx3iE4W0JcafTU0g2hhJX6FBfq5_gHUz4PTnENgMnSyW5naaldHQ0l11rCZldIWFOAQpWYqK0pD6E8viGvvJ0zvn2qN-Tnp48_7r80p2-fv95_ODUglC5NB710XjipnBDjCEeLPchh4Ep6qZRCL-p00Cg7jd4f_eBGDsg1YKdH1Ysb8u6Su6X4e8dczBIy4DzbFeOeDR_0cVBMalWt6mKFFHNO6M2WwmLTo-HMnEGbyTyDNmfQhglTQdfF26cb-7ig-7f2TLYa3l8MWH_6EDCZDAErDxdSxWpcDP-78Rdp25S8</recordid><startdate>201607</startdate><enddate>201607</enddate><creator>Lin, Liyan</creator><creator>Wang, Peipei</creator><creator>Du, Zhenyun</creator><creator>Wang, Wucheng</creator><creator>Cong, Qifei</creator><creator>Zheng, Changping</creator><creator>Jin, Can</creator><creator>Ding, Kan</creator><creator>Shao, Chenghao</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201607</creationdate><title>Structural elucidation of a pectin from flowers of Lonicera japonica and its antipancreatic cancer activity</title><author>Lin, Liyan ; Wang, Peipei ; Du, Zhenyun ; Wang, Wucheng ; Cong, Qifei ; Zheng, Changping ; Jin, Can ; Ding, Kan ; Shao, Chenghao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-2c45df3d56d33bbc9ae4c577165f5666ef3f3d78e528eff9f7db1ce18ce28b643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Antineoplastic Agents, Phytogenic - chemistry</topic><topic>Antineoplastic Agents, Phytogenic - isolation &amp; purification</topic><topic>Antineoplastic Agents, Phytogenic - pharmacology</topic><topic>Antipancreatic cancer cells growth</topic><topic>Carbohydrate Sequence</topic><topic>Cell Line, Tumor</topic><topic>Cell Survival - drug effects</topic><topic>Dose-Response Relationship, Drug</topic><topic>Epithelial Cells - drug effects</topic><topic>Epithelial Cells - pathology</topic><topic>Flowers - chemistry</topic><topic>Humans</topic><topic>Hydrolysis</topic><topic>Lonicera - chemistry</topic><topic>Lonicera japonica</topic><topic>Molecular Weight</topic><topic>Pancreas - drug effects</topic><topic>Pancreas - pathology</topic><topic>Pectins - chemistry</topic><topic>Pectins - isolation &amp; purification</topic><topic>Pectins - pharmacology</topic><topic>Polysaccharide</topic><topic>Rhamnose - chemistry</topic><topic>Rhamnose - isolation &amp; purification</topic><topic>Trifluoroacetic Acid - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lin, Liyan</creatorcontrib><creatorcontrib>Wang, Peipei</creatorcontrib><creatorcontrib>Du, Zhenyun</creatorcontrib><creatorcontrib>Wang, Wucheng</creatorcontrib><creatorcontrib>Cong, Qifei</creatorcontrib><creatorcontrib>Zheng, Changping</creatorcontrib><creatorcontrib>Jin, Can</creatorcontrib><creatorcontrib>Ding, Kan</creatorcontrib><creatorcontrib>Shao, Chenghao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of biological macromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lin, Liyan</au><au>Wang, Peipei</au><au>Du, Zhenyun</au><au>Wang, Wucheng</au><au>Cong, Qifei</au><au>Zheng, Changping</au><au>Jin, Can</au><au>Ding, Kan</au><au>Shao, Chenghao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structural elucidation of a pectin from flowers of Lonicera japonica and its antipancreatic cancer activity</atitle><jtitle>International journal of biological macromolecules</jtitle><addtitle>Int J Biol Macromol</addtitle><date>2016-07</date><risdate>2016</risdate><volume>88</volume><spage>130</spage><epage>137</epage><pages>130-137</pages><issn>0141-8130</issn><eissn>1879-0003</eissn><abstract>[Display omitted] ⿢A new rhamnogalacturonan from flowers of Lonicera japonica is characterized.⿢Backbone is alternate 1,4-α-d-GalpA and 1,2-α-l-Rhap.⿢Branches contain T- and 1,4,6-linked β-d-Galp, T- and 1,5-linked α-l-Araf.⿢Rhamnogalacturonan might inhibit BxPC-3 and PANC-1 pancreatic cancer cells growth. To investigate polysaccharide structure from Lonicera japonica, and study its effects on behavior of pancreatic cells, a homogenous polysaccharide, LJ-02-1, was extracted and purified from flowers of L. japonica by DEAE-cellulose and Sephacryl S-200HR column. The molecular weight was estimated to be 54kDa. Monosaccharide composition was determined to be rhamnose, galacturonic acid, galactose and arabinose in the molar ratio of 10.77:7.88:15.45:65.89 by analyzing the PMP derivatives of the monosaccharides from 2M trifluoracetic acid hydrolysis via HPLC. Based on methylation analysis, partial acid hydrolysis, and NMR spectra, the polysaccharide was elucidated to be a rhamnogalacturonan backbone and substituted partly at C-4 of rhamnose. The branches were determined to be T- and 1,4,6-linked β-d-Galp, T- and 1,5-linked α-l-Araf. The polysaccharide might inhibit BxPC-3 and PANC-1 pancreatic cancer cells growth at the concentration of 1mg/mL with inhibitory ratio of 66.7% and 52.1%, respectively.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>27000440</pmid><doi>10.1016/j.ijbiomac.2016.03.025</doi><tpages>8</tpages></addata></record>
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subjects Antineoplastic Agents, Phytogenic - chemistry
Antineoplastic Agents, Phytogenic - isolation & purification
Antineoplastic Agents, Phytogenic - pharmacology
Antipancreatic cancer cells growth
Carbohydrate Sequence
Cell Line, Tumor
Cell Survival - drug effects
Dose-Response Relationship, Drug
Epithelial Cells - drug effects
Epithelial Cells - pathology
Flowers - chemistry
Humans
Hydrolysis
Lonicera - chemistry
Lonicera japonica
Molecular Weight
Pancreas - drug effects
Pancreas - pathology
Pectins - chemistry
Pectins - isolation & purification
Pectins - pharmacology
Polysaccharide
Rhamnose - chemistry
Rhamnose - isolation & purification
Trifluoroacetic Acid - chemistry
title Structural elucidation of a pectin from flowers of Lonicera japonica and its antipancreatic cancer activity
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