Pparγ Expression in T Cells as a Prognostic Marker of Sepsis
ABSTRACTTranslating murine data to the human situation, we proposed that the level of peroxisome proliferator-activated receptor γ (PPARγ) expression in T cells from septic patients correlates with clinical outcome. In this preliminary report, we analyzed PPARγ mRNA expression in CD3 T cells derived...
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| Veröffentlicht in: | Shock (Augusta, Ga.) Ga.), 2016-06, Vol.45 (6), p.591-597 |
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| creator | Brenneis, Marco Aghajaanpour, Ramin Knape, Tilo Sha, Lisa K Neb, Holger Meybohm, Patrick Zacharowski, Kai Hauser, Ingeborg A Büttner, Stefan Parnham, Michael J Brüne, Bernhard von Knethen, Andreas |
| description | ABSTRACTTranslating murine data to the human situation, we proposed that the level of peroxisome proliferator-activated receptor γ (PPARγ) expression in T cells from septic patients correlates with clinical outcome. In this preliminary report, we analyzed PPARγ mRNA expression in CD3 T cells derived from blood of a very small number of septic patients (n = 18) on various days up to 2 weeks after the initial diagnosis. CD3 T cell count was determined by flow cytometry. T cells from n = 11 healthy donors were included as controls. Maximal PPARγ mRNA expression was observed on the day of sepsis diagnosis (day 0; 5,896 ± 1,523 copies PPARγ mRNA/25 ng mRNA, P |
| doi_str_mv | 10.1097/SHK.0000000000000568 |
| format | Article |
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In this preliminary report, we analyzed PPARγ mRNA expression in CD3 T cells derived from blood of a very small number of septic patients (n = 18) on various days up to 2 weeks after the initial diagnosis. CD3 T cell count was determined by flow cytometry. T cells from n = 11 healthy donors were included as controls. Maximal PPARγ mRNA expression was observed on the day of sepsis diagnosis (day 0; 5,896 ± 1,523 copies PPARγ mRNA/25 ng mRNA, P < 0.05 vs. controls). In contrast, the number of CD3 T cells was significantly decreased in septic patients compared with healthy controls (296 ± 31 vs. 1,803 ± 134 T cells/μL blood, P < 0.001). Setting two arbitrary limitspatients with a PPARγ expression in T cells higher than 7,000 copies/25 ng mRNA, of whom five of six patients died during the ICU stay, and patients with a T cell count below 100 T cells/μL blood, of whom five of eight patients died, we identified a correlation between sepsis survival and low T cell number, paired with high T cell-specific PPARγ expression. Among all 18 sepsis patients, four fulfilled the criteria for both arbitrary settings and all four of these patients subsequently died. We suggest that both high PPARγ expression in T cells and low absolute T cell number in blood of septic patients may have the potential as a new prognostic marker for a poor sepsis outcome.</description><identifier>ISSN: 1073-2322</identifier><identifier>EISSN: 1540-0514</identifier><identifier>DOI: 10.1097/SHK.0000000000000568</identifier><identifier>PMID: 26796570</identifier><language>eng</language><publisher>United States: by the Shock Society</publisher><subject>Adolescent ; Adult ; Aged ; Biomarkers - blood ; Case-Control Studies ; CD3 Complex - metabolism ; Female ; Follow-Up Studies ; Hospitals, University ; Humans ; Intensive Care Units ; Lymphocyte Count ; Male ; Middle Aged ; PPAR gamma - blood ; Predictive Value of Tests ; Prognosis ; Sensitivity and Specificity ; Sepsis - blood ; Sepsis - diagnosis ; Sepsis - mortality ; Survival Analysis ; T-Lymphocytes - metabolism</subject><ispartof>Shock (Augusta, Ga.), 2016-06, Vol.45 (6), p.591-597</ispartof><rights>2016 by the Shock Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3568-50deb2de8e74bb5c1adacc6b2942129ac63a5056f810654d9691c7eff34b9aa03</citedby><cites>FETCH-LOGICAL-c3568-50deb2de8e74bb5c1adacc6b2942129ac63a5056f810654d9691c7eff34b9aa03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26796570$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brenneis, Marco</creatorcontrib><creatorcontrib>Aghajaanpour, Ramin</creatorcontrib><creatorcontrib>Knape, Tilo</creatorcontrib><creatorcontrib>Sha, Lisa K</creatorcontrib><creatorcontrib>Neb, Holger</creatorcontrib><creatorcontrib>Meybohm, Patrick</creatorcontrib><creatorcontrib>Zacharowski, Kai</creatorcontrib><creatorcontrib>Hauser, Ingeborg A</creatorcontrib><creatorcontrib>Büttner, Stefan</creatorcontrib><creatorcontrib>Parnham, Michael J</creatorcontrib><creatorcontrib>Brüne, Bernhard</creatorcontrib><creatorcontrib>von Knethen, Andreas</creatorcontrib><title>Pparγ Expression in T Cells as a Prognostic Marker of Sepsis</title><title>Shock (Augusta, Ga.)</title><addtitle>Shock</addtitle><description>ABSTRACTTranslating murine data to the human situation, we proposed that the level of peroxisome proliferator-activated receptor γ (PPARγ) expression in T cells from septic patients correlates with clinical outcome. In this preliminary report, we analyzed PPARγ mRNA expression in CD3 T cells derived from blood of a very small number of septic patients (n = 18) on various days up to 2 weeks after the initial diagnosis. CD3 T cell count was determined by flow cytometry. T cells from n = 11 healthy donors were included as controls. Maximal PPARγ mRNA expression was observed on the day of sepsis diagnosis (day 0; 5,896 ± 1,523 copies PPARγ mRNA/25 ng mRNA, P < 0.05 vs. controls). In contrast, the number of CD3 T cells was significantly decreased in septic patients compared with healthy controls (296 ± 31 vs. 1,803 ± 134 T cells/μL blood, P < 0.001). Setting two arbitrary limitspatients with a PPARγ expression in T cells higher than 7,000 copies/25 ng mRNA, of whom five of six patients died during the ICU stay, and patients with a T cell count below 100 T cells/μL blood, of whom five of eight patients died, we identified a correlation between sepsis survival and low T cell number, paired with high T cell-specific PPARγ expression. Among all 18 sepsis patients, four fulfilled the criteria for both arbitrary settings and all four of these patients subsequently died. We suggest that both high PPARγ expression in T cells and low absolute T cell number in blood of septic patients may have the potential as a new prognostic marker for a poor sepsis outcome.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Biomarkers - blood</subject><subject>Case-Control Studies</subject><subject>CD3 Complex - metabolism</subject><subject>Female</subject><subject>Follow-Up Studies</subject><subject>Hospitals, University</subject><subject>Humans</subject><subject>Intensive Care Units</subject><subject>Lymphocyte Count</subject><subject>Male</subject><subject>Middle Aged</subject><subject>PPAR gamma - blood</subject><subject>Predictive Value of Tests</subject><subject>Prognosis</subject><subject>Sensitivity and Specificity</subject><subject>Sepsis - blood</subject><subject>Sepsis - diagnosis</subject><subject>Sepsis - mortality</subject><subject>Survival Analysis</subject><subject>T-Lymphocytes - metabolism</subject><issn>1073-2322</issn><issn>1540-0514</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEFOwzAQRS0EoqVwA4S8ZJNiO3YcL1igqlBEEZVa1pbjODQ0jYOdqHAu7sGZMGpBiAWjkWYW_898PQBOMRpiJPjFfHI3RL-LJeke6GNGUYQYpvthRzyOSExIDxx5_4wQobHgh6BHEi4SxlEfXM4a5T7e4fi1ccb70tawrOECjkxVeahCw5mzT7X1banhvXIr46At4Nw0vvTH4KBQlTcnuzkAj9fjxWgSTR9ubkdX00jHIVXEUG4ykpvUcJplTGOVK62TjAhKMBFKJ7FiIX-RYpQwmotEYM1NUcQ0E0qheADOt3cbZ18641u5Lr0OEVVtbOcl5qngTCCEg5RupdpZ750pZOPKtXJvEiP5BU4GcPIvuGA7233osrXJf0zfpIIg3Qo2tmqN86uq2xgnl0ZV7fL_25_hWnko</recordid><startdate>201606</startdate><enddate>201606</enddate><creator>Brenneis, Marco</creator><creator>Aghajaanpour, Ramin</creator><creator>Knape, Tilo</creator><creator>Sha, Lisa K</creator><creator>Neb, Holger</creator><creator>Meybohm, Patrick</creator><creator>Zacharowski, Kai</creator><creator>Hauser, Ingeborg A</creator><creator>Büttner, Stefan</creator><creator>Parnham, Michael J</creator><creator>Brüne, Bernhard</creator><creator>von Knethen, Andreas</creator><general>by the Shock Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201606</creationdate><title>Pparγ Expression in T Cells as a Prognostic Marker of Sepsis</title><author>Brenneis, Marco ; Aghajaanpour, Ramin ; Knape, Tilo ; Sha, Lisa K ; Neb, Holger ; Meybohm, Patrick ; Zacharowski, Kai ; Hauser, Ingeborg A ; Büttner, Stefan ; Parnham, Michael J ; Brüne, Bernhard ; von Knethen, Andreas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3568-50deb2de8e74bb5c1adacc6b2942129ac63a5056f810654d9691c7eff34b9aa03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Biomarkers - blood</topic><topic>Case-Control Studies</topic><topic>CD3 Complex - metabolism</topic><topic>Female</topic><topic>Follow-Up Studies</topic><topic>Hospitals, University</topic><topic>Humans</topic><topic>Intensive Care Units</topic><topic>Lymphocyte Count</topic><topic>Male</topic><topic>Middle Aged</topic><topic>PPAR gamma - blood</topic><topic>Predictive Value of Tests</topic><topic>Prognosis</topic><topic>Sensitivity and Specificity</topic><topic>Sepsis - blood</topic><topic>Sepsis - diagnosis</topic><topic>Sepsis - mortality</topic><topic>Survival Analysis</topic><topic>T-Lymphocytes - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brenneis, Marco</creatorcontrib><creatorcontrib>Aghajaanpour, Ramin</creatorcontrib><creatorcontrib>Knape, Tilo</creatorcontrib><creatorcontrib>Sha, Lisa K</creatorcontrib><creatorcontrib>Neb, Holger</creatorcontrib><creatorcontrib>Meybohm, Patrick</creatorcontrib><creatorcontrib>Zacharowski, Kai</creatorcontrib><creatorcontrib>Hauser, Ingeborg A</creatorcontrib><creatorcontrib>Büttner, Stefan</creatorcontrib><creatorcontrib>Parnham, Michael J</creatorcontrib><creatorcontrib>Brüne, Bernhard</creatorcontrib><creatorcontrib>von Knethen, Andreas</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Shock (Augusta, Ga.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brenneis, Marco</au><au>Aghajaanpour, Ramin</au><au>Knape, Tilo</au><au>Sha, Lisa K</au><au>Neb, Holger</au><au>Meybohm, Patrick</au><au>Zacharowski, Kai</au><au>Hauser, Ingeborg A</au><au>Büttner, Stefan</au><au>Parnham, Michael J</au><au>Brüne, Bernhard</au><au>von Knethen, Andreas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pparγ Expression in T Cells as a Prognostic Marker of Sepsis</atitle><jtitle>Shock (Augusta, Ga.)</jtitle><addtitle>Shock</addtitle><date>2016-06</date><risdate>2016</risdate><volume>45</volume><issue>6</issue><spage>591</spage><epage>597</epage><pages>591-597</pages><issn>1073-2322</issn><eissn>1540-0514</eissn><abstract>ABSTRACTTranslating murine data to the human situation, we proposed that the level of peroxisome proliferator-activated receptor γ (PPARγ) expression in T cells from septic patients correlates with clinical outcome. In this preliminary report, we analyzed PPARγ mRNA expression in CD3 T cells derived from blood of a very small number of septic patients (n = 18) on various days up to 2 weeks after the initial diagnosis. CD3 T cell count was determined by flow cytometry. T cells from n = 11 healthy donors were included as controls. Maximal PPARγ mRNA expression was observed on the day of sepsis diagnosis (day 0; 5,896 ± 1,523 copies PPARγ mRNA/25 ng mRNA, P < 0.05 vs. controls). In contrast, the number of CD3 T cells was significantly decreased in septic patients compared with healthy controls (296 ± 31 vs. 1,803 ± 134 T cells/μL blood, P < 0.001). Setting two arbitrary limitspatients with a PPARγ expression in T cells higher than 7,000 copies/25 ng mRNA, of whom five of six patients died during the ICU stay, and patients with a T cell count below 100 T cells/μL blood, of whom five of eight patients died, we identified a correlation between sepsis survival and low T cell number, paired with high T cell-specific PPARγ expression. Among all 18 sepsis patients, four fulfilled the criteria for both arbitrary settings and all four of these patients subsequently died. We suggest that both high PPARγ expression in T cells and low absolute T cell number in blood of septic patients may have the potential as a new prognostic marker for a poor sepsis outcome.</abstract><cop>United States</cop><pub>by the Shock Society</pub><pmid>26796570</pmid><doi>10.1097/SHK.0000000000000568</doi><tpages>7</tpages></addata></record> |
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| subjects | Adolescent Adult Aged Biomarkers - blood Case-Control Studies CD3 Complex - metabolism Female Follow-Up Studies Hospitals, University Humans Intensive Care Units Lymphocyte Count Male Middle Aged PPAR gamma - blood Predictive Value of Tests Prognosis Sensitivity and Specificity Sepsis - blood Sepsis - diagnosis Sepsis - mortality Survival Analysis T-Lymphocytes - metabolism |
| title | Pparγ Expression in T Cells as a Prognostic Marker of Sepsis |
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