Protein Kinase C Is Involved in the Regulation of hairless mRNA Expression during Mouse Keratinocyte Differentiation
The hairless (hr) gene is a putative transcriptional factor whose mutations lead to hair loss in animals and humans. As a step toward understanding the role of the hr gene, we investigated the expression of hr mRNA in mouse keratinocyte differentiation. Treatment of mouse primary keratinocyte cultur...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2001-06, Vol.284 (1), p.99-105 |
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| creator | Wan, Xiaozhu Kong, Juan Li, Yan Chun |
| description | The hairless (hr) gene is a putative transcriptional factor whose mutations lead to hair loss in animals and humans. As a step toward understanding the role of the hr gene, we investigated the expression of hr mRNA in mouse keratinocyte differentiation. Treatment of mouse primary keratinocyte cultures with phorbol-12-myristate-13-acetate (PMA) reduced DNA synthesis and sequentially induced an up-regulation of p21Cip1/WAF1 (p21), hr and involucrin (inv) mRNAs in a time-dependent fashion, suggesting that an increase in hr gene expression is associated with keratinocyte differentiation. This up-regulation was blocked by the RNA synthesis inhibitor actinomycin D. However, an increase in hr mRNA, but not in inv mRNA, was seen in cells treated with the protein synthesis inhibitor cycloheximide, suggesting that new protein synthesis is involved in the suppression of hr transcription or in the degradation of hr mRNA in the steady state. The up-regulation of hr mRNA expression by PMA was blocked by the protein kinase C (PKC) inhibitor, GF109203X. These data indicate that PKC activation is involved in the up-regulation of hr mRNA expression during mouse keratinocyte differentiation. |
| doi_str_mv | 10.1006/bbrc.2001.4923 |
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As a step toward understanding the role of the hr gene, we investigated the expression of hr mRNA in mouse keratinocyte differentiation. Treatment of mouse primary keratinocyte cultures with phorbol-12-myristate-13-acetate (PMA) reduced DNA synthesis and sequentially induced an up-regulation of p21Cip1/WAF1 (p21), hr and involucrin (inv) mRNAs in a time-dependent fashion, suggesting that an increase in hr gene expression is associated with keratinocyte differentiation. This up-regulation was blocked by the RNA synthesis inhibitor actinomycin D. However, an increase in hr mRNA, but not in inv mRNA, was seen in cells treated with the protein synthesis inhibitor cycloheximide, suggesting that new protein synthesis is involved in the suppression of hr transcription or in the degradation of hr mRNA in the steady state. The up-regulation of hr mRNA expression by PMA was blocked by the protein kinase C (PKC) inhibitor, GF109203X. These data indicate that PKC activation is involved in the up-regulation of hr mRNA expression during mouse keratinocyte differentiation.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.2001.4923</identifier><identifier>PMID: 11374877</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cell Differentiation - physiology ; Cells, Cultured ; Cyclin-Dependent Kinase Inhibitor p21 ; Cyclins - genetics ; Cyclins - metabolism ; cycloheximide ; DNA - metabolism ; Dose-Response Relationship, Drug ; Enzyme Activators - pharmacology ; Enzyme Inhibitors - pharmacology ; Gene Expression Regulation ; GF109203X ; hairless gene ; hr gene ; involucrin ; keratinocytes ; Keratinocytes - cytology ; Keratinocytes - drug effects ; Keratinocytes - metabolism ; Mice ; phorbol ester ; protein kinase C ; Protein Kinase C - antagonists & inhibitors ; Protein Kinase C - metabolism ; Protein Precursors - genetics ; Protein Precursors - metabolism ; Protein Synthesis Inhibitors - pharmacology ; Proteins - genetics ; Proteins - metabolism ; RNA, Messenger - metabolism ; Tetradecanoylphorbol Acetate - pharmacology ; Transcription Factors ; Up-Regulation</subject><ispartof>Biochemical and biophysical research communications, 2001-06, Vol.284 (1), p.99-105</ispartof><rights>2001 Academic Press</rights><rights>Copyright 2001 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c371t-3b12c7a8a6a946e1392658a074755fed225f82195e3f989124140cb4ba960ada3</citedby><cites>FETCH-LOGICAL-c371t-3b12c7a8a6a946e1392658a074755fed225f82195e3f989124140cb4ba960ada3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/bbrc.2001.4923$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11374877$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wan, Xiaozhu</creatorcontrib><creatorcontrib>Kong, Juan</creatorcontrib><creatorcontrib>Li, Yan Chun</creatorcontrib><title>Protein Kinase C Is Involved in the Regulation of hairless mRNA Expression during Mouse Keratinocyte Differentiation</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>The hairless (hr) gene is a putative transcriptional factor whose mutations lead to hair loss in animals and humans. As a step toward understanding the role of the hr gene, we investigated the expression of hr mRNA in mouse keratinocyte differentiation. Treatment of mouse primary keratinocyte cultures with phorbol-12-myristate-13-acetate (PMA) reduced DNA synthesis and sequentially induced an up-regulation of p21Cip1/WAF1 (p21), hr and involucrin (inv) mRNAs in a time-dependent fashion, suggesting that an increase in hr gene expression is associated with keratinocyte differentiation. This up-regulation was blocked by the RNA synthesis inhibitor actinomycin D. However, an increase in hr mRNA, but not in inv mRNA, was seen in cells treated with the protein synthesis inhibitor cycloheximide, suggesting that new protein synthesis is involved in the suppression of hr transcription or in the degradation of hr mRNA in the steady state. The up-regulation of hr mRNA expression by PMA was blocked by the protein kinase C (PKC) inhibitor, GF109203X. These data indicate that PKC activation is involved in the up-regulation of hr mRNA expression during mouse keratinocyte differentiation.</description><subject>Animals</subject><subject>Cell Differentiation - physiology</subject><subject>Cells, Cultured</subject><subject>Cyclin-Dependent Kinase Inhibitor p21</subject><subject>Cyclins - genetics</subject><subject>Cyclins - metabolism</subject><subject>cycloheximide</subject><subject>DNA - metabolism</subject><subject>Dose-Response Relationship, Drug</subject><subject>Enzyme Activators - pharmacology</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Gene Expression Regulation</subject><subject>GF109203X</subject><subject>hairless gene</subject><subject>hr gene</subject><subject>involucrin</subject><subject>keratinocytes</subject><subject>Keratinocytes - cytology</subject><subject>Keratinocytes - drug effects</subject><subject>Keratinocytes - metabolism</subject><subject>Mice</subject><subject>phorbol ester</subject><subject>protein kinase C</subject><subject>Protein Kinase C - antagonists & inhibitors</subject><subject>Protein Kinase C - metabolism</subject><subject>Protein Precursors - genetics</subject><subject>Protein Precursors - metabolism</subject><subject>Protein Synthesis Inhibitors - pharmacology</subject><subject>Proteins - genetics</subject><subject>Proteins - metabolism</subject><subject>RNA, Messenger - metabolism</subject><subject>Tetradecanoylphorbol Acetate - pharmacology</subject><subject>Transcription Factors</subject><subject>Up-Regulation</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kEtv3CAURlGVqpkk3XZZscrOUy7GD5bR5DVK-lCUSt0hjK8TKg9MAI-afx_cGamrrtDlnu8IPkI-AVsCY_WXrgtmyRmDpZC8fEcWwCQrODBxRBYsEwWX8OuYnMT4O1MgavmBHAOUjWibZkHSj-ATWkfvrNMR6YquI127nR932NN8n56RPuDTNOpkvaN-oM_ahhFjpJuHbxf06s825GHe9VOw7ol-9VMW3WHICefNa0J6aYcBA7pk_1rOyPtBjxE_Hs5T8vP66nF1W9x_v1mvLu4LUzaQirIDbhrd6lpLUSOUktdVq1kjmqoasOe8GloOssJykK0ELkAw04lOy5rpXpen5Hzv3Qb_MmFMamOjwXHUDvMjFTRziosMLvegCT7GgIPaBrvR4VUBU3PPau5ZzT2ruecc-HwwT90G-3_4odgMtHsA8_92FoOKxqIz2NuAJqne2_-53wBxMYzB</recordid><startdate>20010601</startdate><enddate>20010601</enddate><creator>Wan, Xiaozhu</creator><creator>Kong, Juan</creator><creator>Li, Yan Chun</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope></search><sort><creationdate>20010601</creationdate><title>Protein Kinase C Is Involved in the Regulation of hairless mRNA Expression during Mouse Keratinocyte Differentiation</title><author>Wan, Xiaozhu ; Kong, Juan ; Li, Yan Chun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c371t-3b12c7a8a6a946e1392658a074755fed225f82195e3f989124140cb4ba960ada3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Cell Differentiation - physiology</topic><topic>Cells, Cultured</topic><topic>Cyclin-Dependent Kinase Inhibitor p21</topic><topic>Cyclins - genetics</topic><topic>Cyclins - metabolism</topic><topic>cycloheximide</topic><topic>DNA - metabolism</topic><topic>Dose-Response Relationship, Drug</topic><topic>Enzyme Activators - pharmacology</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Gene Expression Regulation</topic><topic>GF109203X</topic><topic>hairless gene</topic><topic>hr gene</topic><topic>involucrin</topic><topic>keratinocytes</topic><topic>Keratinocytes - cytology</topic><topic>Keratinocytes - drug effects</topic><topic>Keratinocytes - metabolism</topic><topic>Mice</topic><topic>phorbol ester</topic><topic>protein kinase C</topic><topic>Protein Kinase C - antagonists & inhibitors</topic><topic>Protein Kinase C - metabolism</topic><topic>Protein Precursors - genetics</topic><topic>Protein Precursors - metabolism</topic><topic>Protein Synthesis Inhibitors - pharmacology</topic><topic>Proteins - genetics</topic><topic>Proteins - metabolism</topic><topic>RNA, Messenger - metabolism</topic><topic>Tetradecanoylphorbol Acetate - pharmacology</topic><topic>Transcription Factors</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wan, Xiaozhu</creatorcontrib><creatorcontrib>Kong, Juan</creatorcontrib><creatorcontrib>Li, Yan Chun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wan, Xiaozhu</au><au>Kong, Juan</au><au>Li, Yan Chun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protein Kinase C Is Involved in the Regulation of hairless mRNA Expression during Mouse Keratinocyte Differentiation</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2001-06-01</date><risdate>2001</risdate><volume>284</volume><issue>1</issue><spage>99</spage><epage>105</epage><pages>99-105</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>The hairless (hr) gene is a putative transcriptional factor whose mutations lead to hair loss in animals and humans. As a step toward understanding the role of the hr gene, we investigated the expression of hr mRNA in mouse keratinocyte differentiation. Treatment of mouse primary keratinocyte cultures with phorbol-12-myristate-13-acetate (PMA) reduced DNA synthesis and sequentially induced an up-regulation of p21Cip1/WAF1 (p21), hr and involucrin (inv) mRNAs in a time-dependent fashion, suggesting that an increase in hr gene expression is associated with keratinocyte differentiation. This up-regulation was blocked by the RNA synthesis inhibitor actinomycin D. However, an increase in hr mRNA, but not in inv mRNA, was seen in cells treated with the protein synthesis inhibitor cycloheximide, suggesting that new protein synthesis is involved in the suppression of hr transcription or in the degradation of hr mRNA in the steady state. The up-regulation of hr mRNA expression by PMA was blocked by the protein kinase C (PKC) inhibitor, GF109203X. These data indicate that PKC activation is involved in the up-regulation of hr mRNA expression during mouse keratinocyte differentiation.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>11374877</pmid><doi>10.1006/bbrc.2001.4923</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals Cell Differentiation - physiology Cells, Cultured Cyclin-Dependent Kinase Inhibitor p21 Cyclins - genetics Cyclins - metabolism cycloheximide DNA - metabolism Dose-Response Relationship, Drug Enzyme Activators - pharmacology Enzyme Inhibitors - pharmacology Gene Expression Regulation GF109203X hairless gene hr gene involucrin keratinocytes Keratinocytes - cytology Keratinocytes - drug effects Keratinocytes - metabolism Mice phorbol ester protein kinase C Protein Kinase C - antagonists & inhibitors Protein Kinase C - metabolism Protein Precursors - genetics Protein Precursors - metabolism Protein Synthesis Inhibitors - pharmacology Proteins - genetics Proteins - metabolism RNA, Messenger - metabolism Tetradecanoylphorbol Acetate - pharmacology Transcription Factors Up-Regulation |
| title | Protein Kinase C Is Involved in the Regulation of hairless mRNA Expression during Mouse Keratinocyte Differentiation |
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