Highly toxic and broad-spectrum insecticidal Bacillus thuringiensis engineered by using the transposon Tn917 and protoplast fusion

The chromosome of the Bacillus thuringiensis strain S184 that was toxic against the third instar larvae of Spodoptera litura with the LC(50) of 9.74 microg/ml was successfully integrated into two genes of cyt1Aa and cry11Aa using the transposon Tn917, yielding the primary engineered strain TnX. The...

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Veröffentlicht in:	Current microbiology 2001-08, Vol.43 (2), p.112-119
Hauptverfasser:	Yu, J, Pang, Y, Tang, M, Xie, R, Tan, L, Zeng, S, Yuan, M, Liu, J
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacillus thuringiensis Bacillus thuringiensis - genetics Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacterial Toxins Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Biological Assay Chromosomes cry11Aa gene Culex - microbiology Culex - physiology cyt1Aa gene DNA Transposable Elements Endotoxins - genetics Endotoxins - metabolism Fundamental and applied biological sciences. Psychology Genetic Engineering Hemolysin Proteins Insecticides Larva - microbiology Larva - physiology Larvae Metabolism. Enzymes Microbiology Pest Control, Biological Plasmids Spodoptera - microbiology Spodoptera - physiology Transformation, Genetic
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