Production of intergeneric somatic hybrids of chrysanthemum [ Dendranthema × grandiflorum (Ramat.) Kitamura] and wormwood ( Artemisia sieversiana J. F. Ehrh. ex. Willd) with rust ( Puccinia horiana Henning) resistance by electrofusion of protoplasts

We produced intergeneric somatic hybrids between chrysanthemum [ Dendranthema× grandiflorum (Ramat.) Kitamura] and wormwood ( Artemisia sieversiana J. F. Ehrh. ex. Willd) by electrofusion of mesophyll protoplasts. Chrysanthemum protoplasts were inactivated with iodoacetamide for 15 min at 5 °C and e...

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Veröffentlicht in:	Plant science (Limerick) 2004-03, Vol.166 (3), p.695-702
Hauptverfasser:	Furuta, Hideo, Shinoyama, Harue, Nomura, Yukio, Maeda, Masuo, Makara, Kouichi
Format:	Artikel
Sprache:	eng
Schlagworte:	Agronomy. Soil science and plant productions Artemisia Artemisia sieversiana Biological and medical sciences chromosomes Chrysanthemum morifolium Compositae family Dendranthema grandiflorum DNA Electrofusion Fundamental and applied biological sciences. Psychology Genetics and breeding of economic plants Haploidy, in vitro culture applications, somatic hybrids in vitro regeneration intergeneric hybridization Intergenetic somatic hybrid micropropagation ornamental plants phenotypic variation Plant breeding: fundamental aspects and methodology plant morphology plant pathogenic fungi pollen germination protoplast fusion Puccinia horiana resistance mechanisms rooting rust diseases Rust resistance somatic hybridization
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