Mitotic checkpoint genes hBUB1, hBUB1B, hBUB3 and TTK in human bladder cancer, screening for mutations and loss of heterozygosity
Chromosomal instability is common in bladder cancer and could be caused by mutations of mitotic checkpoint genes. Therefore we screened for mutations of the mitotic checkpoint genes hBUB1, hBUB1B, hBUB3 and TTK in six aneuploid bladder cancer cell lines and 15 human bladder tumours. The screening wa...
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| Veröffentlicht in: | Carcinogenesis (New York) 2001-05, Vol.22 (5), p.813-815 |
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| container_title | Carcinogenesis (New York) |
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| creator | Olesen, Sanne H. Thykjaer, Thomas Ørntoft, Torben F. |
| description | Chromosomal instability is common in bladder cancer and could be caused by mutations of mitotic checkpoint genes. Therefore we screened for mutations of the mitotic checkpoint genes hBUB1, hBUB1B, hBUB3 and TTK in six aneuploid bladder cancer cell lines and 15 human bladder tumours. The screening was performed by sequence analysis of the entire coding regions of the four genes. No mutations were detected in any of the four genes. We detected several sequence variations in hBUB1, hBUB1B and TTK both new and previously published. The genetic stability of the four gene loci were tested by loss of heterozygosity (LOH) analysis in the 15 patient samples, showing one LOH for each of the hBUB1B, hBUB3 and TTK loci (6.7%) of the cases, all in different tumour samples. No LOH was detected at hBUB1. We conclude that both mutational inactivation, and loss of one allele, of the examined mitotic checkpoint genes are relatively uncommon. |
| doi_str_mv | 10.1093/carcin/22.5.813 |
| format | Article |
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Therefore we screened for mutations of the mitotic checkpoint genes hBUB1, hBUB1B, hBUB3 and TTK in six aneuploid bladder cancer cell lines and 15 human bladder tumours. The screening was performed by sequence analysis of the entire coding regions of the four genes. No mutations were detected in any of the four genes. We detected several sequence variations in hBUB1, hBUB1B and TTK both new and previously published. The genetic stability of the four gene loci were tested by loss of heterozygosity (LOH) analysis in the 15 patient samples, showing one LOH for each of the hBUB1B, hBUB3 and TTK loci (6.7%) of the cases, all in different tumour samples. No LOH was detected at hBUB1. We conclude that both mutational inactivation, and loss of one allele, of the examined mitotic checkpoint genes are relatively uncommon.</description><identifier>ISSN: 0143-3334</identifier><identifier>ISSN: 1460-2180</identifier><identifier>EISSN: 1460-2180</identifier><identifier>DOI: 10.1093/carcin/22.5.813</identifier><identifier>PMID: 11323402</identifier><identifier>CODEN: CRNGDP</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>anaphase promoting complex ; APC ; Biological and medical sciences ; BUB ; budding uninhibited by benomyl ; Genes, cdc ; hBUB1 gene ; hBUB1B gene ; hBUB3 gene ; Humans ; LOH ; Loss of Heterozygosity ; MAD ; Medical sciences ; mitotic arrest deficiency ; Mutation ; Nephrology. Urinary tract diseases ; TTK gene ; Tumors of the urinary system ; Urinary Bladder Neoplasms - genetics ; Urinary tract. Prostate gland</subject><ispartof>Carcinogenesis (New York), 2001-05, Vol.22 (5), p.813-815</ispartof><rights>2001 INIST-CNRS</rights><rights>Copyright Oxford University Press(England) May 2001</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c525t-c1eb6803120de123531d1ef27a71adb694483bf6642b55e2817f37a1bfd7c6f53</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1023131$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11323402$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Olesen, Sanne H.</creatorcontrib><creatorcontrib>Thykjaer, Thomas</creatorcontrib><creatorcontrib>Ørntoft, Torben F.</creatorcontrib><title>Mitotic checkpoint genes hBUB1, hBUB1B, hBUB3 and TTK in human bladder cancer, screening for mutations and loss of heterozygosity</title><title>Carcinogenesis (New York)</title><addtitle>Carcinogenesis</addtitle><description>Chromosomal instability is common in bladder cancer and could be caused by mutations of mitotic checkpoint genes. Therefore we screened for mutations of the mitotic checkpoint genes hBUB1, hBUB1B, hBUB3 and TTK in six aneuploid bladder cancer cell lines and 15 human bladder tumours. The screening was performed by sequence analysis of the entire coding regions of the four genes. No mutations were detected in any of the four genes. We detected several sequence variations in hBUB1, hBUB1B and TTK both new and previously published. The genetic stability of the four gene loci were tested by loss of heterozygosity (LOH) analysis in the 15 patient samples, showing one LOH for each of the hBUB1B, hBUB3 and TTK loci (6.7%) of the cases, all in different tumour samples. No LOH was detected at hBUB1. We conclude that both mutational inactivation, and loss of one allele, of the examined mitotic checkpoint genes are relatively uncommon.</description><subject>anaphase promoting complex</subject><subject>APC</subject><subject>Biological and medical sciences</subject><subject>BUB</subject><subject>budding uninhibited by benomyl</subject><subject>Genes, cdc</subject><subject>hBUB1 gene</subject><subject>hBUB1B gene</subject><subject>hBUB3 gene</subject><subject>Humans</subject><subject>LOH</subject><subject>Loss of Heterozygosity</subject><subject>MAD</subject><subject>Medical sciences</subject><subject>mitotic arrest deficiency</subject><subject>Mutation</subject><subject>Nephrology. Urinary tract diseases</subject><subject>TTK gene</subject><subject>Tumors of the urinary system</subject><subject>Urinary Bladder Neoplasms - genetics</subject><subject>Urinary tract. Prostate gland</subject><issn>0143-3334</issn><issn>1460-2180</issn><issn>1460-2180</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkc2P0zAQxSMEYsvCmRuyEOK0aT2eOE6PdMXSFV1xoCshLpbjOK13E7vYiUS58Z9jNhUgTu8wv3nz8bLsJdA50CUutAraugVjcz6vAB9lMyhKmjOo6ONsRqHAHBGLs-xZjHeUQol8-TQ7A0CGBWWz7OeNHfxgNdF7o-8P3rqB7IwzkexXtyu4mGQ1KRLlGrLdfiTWkf3YK0fqTjWNCUQrp024IFEHY5x1O9L6QPpxUIP1Lj40dj5G4luyN4MJ_sdx56Mdjs-zJ63qonlx0vPs9ur99nKdbz59uL58t8k1Z3zINZi6rCgCo40BhhyhAdMyoQSopi6XRVFh3ZZlwWrODatAtCgU1G0jdNlyPM_eTr6H4L-NJg6yt1GbrlPO-DFKEFUlhGAJfP0feOfH4NJuksESAXlZJGgxQTqkq4Jp5SHYXoWjBCp_RyOnaCRjkssUTep4dbId6940f_lTFgl4cwJU1KprQ3qpjf_4sjQbEpZPmI2D-f6nrMK9LAUKLtdfvsr16vNVsaE3EvEXRVSlSg</recordid><startdate>20010501</startdate><enddate>20010501</enddate><creator>Olesen, Sanne H.</creator><creator>Thykjaer, Thomas</creator><creator>Ørntoft, Torben F.</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20010501</creationdate><title>Mitotic checkpoint genes hBUB1, hBUB1B, hBUB3 and TTK in human bladder cancer, screening for mutations and loss of heterozygosity</title><author>Olesen, Sanne H. ; Thykjaer, Thomas ; Ørntoft, Torben F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c525t-c1eb6803120de123531d1ef27a71adb694483bf6642b55e2817f37a1bfd7c6f53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>anaphase promoting complex</topic><topic>APC</topic><topic>Biological and medical sciences</topic><topic>BUB</topic><topic>budding uninhibited by benomyl</topic><topic>Genes, cdc</topic><topic>hBUB1 gene</topic><topic>hBUB1B gene</topic><topic>hBUB3 gene</topic><topic>Humans</topic><topic>LOH</topic><topic>Loss of Heterozygosity</topic><topic>MAD</topic><topic>Medical sciences</topic><topic>mitotic arrest deficiency</topic><topic>Mutation</topic><topic>Nephrology. Urinary tract diseases</topic><topic>TTK gene</topic><topic>Tumors of the urinary system</topic><topic>Urinary Bladder Neoplasms - genetics</topic><topic>Urinary tract. Prostate gland</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Olesen, Sanne H.</creatorcontrib><creatorcontrib>Thykjaer, Thomas</creatorcontrib><creatorcontrib>Ørntoft, Torben F.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Carcinogenesis (New York)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Olesen, Sanne H.</au><au>Thykjaer, Thomas</au><au>Ørntoft, Torben F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mitotic checkpoint genes hBUB1, hBUB1B, hBUB3 and TTK in human bladder cancer, screening for mutations and loss of heterozygosity</atitle><jtitle>Carcinogenesis (New York)</jtitle><addtitle>Carcinogenesis</addtitle><date>2001-05-01</date><risdate>2001</risdate><volume>22</volume><issue>5</issue><spage>813</spage><epage>815</epage><pages>813-815</pages><issn>0143-3334</issn><issn>1460-2180</issn><eissn>1460-2180</eissn><coden>CRNGDP</coden><abstract>Chromosomal instability is common in bladder cancer and could be caused by mutations of mitotic checkpoint genes. Therefore we screened for mutations of the mitotic checkpoint genes hBUB1, hBUB1B, hBUB3 and TTK in six aneuploid bladder cancer cell lines and 15 human bladder tumours. The screening was performed by sequence analysis of the entire coding regions of the four genes. No mutations were detected in any of the four genes. We detected several sequence variations in hBUB1, hBUB1B and TTK both new and previously published. The genetic stability of the four gene loci were tested by loss of heterozygosity (LOH) analysis in the 15 patient samples, showing one LOH for each of the hBUB1B, hBUB3 and TTK loci (6.7%) of the cases, all in different tumour samples. No LOH was detected at hBUB1. We conclude that both mutational inactivation, and loss of one allele, of the examined mitotic checkpoint genes are relatively uncommon.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>11323402</pmid><doi>10.1093/carcin/22.5.813</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | anaphase promoting complex APC Biological and medical sciences BUB budding uninhibited by benomyl Genes, cdc hBUB1 gene hBUB1B gene hBUB3 gene Humans LOH Loss of Heterozygosity MAD Medical sciences mitotic arrest deficiency Mutation Nephrology. Urinary tract diseases TTK gene Tumors of the urinary system Urinary Bladder Neoplasms - genetics Urinary tract. Prostate gland |
| title | Mitotic checkpoint genes hBUB1, hBUB1B, hBUB3 and TTK in human bladder cancer, screening for mutations and loss of heterozygosity |
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