Screening and validation of long non-coding RNAs in brain tissue of inflammation-induced preterm mice
To investigate the association between long non-coding RNAs (lncRNAs) and brain injury in inflammation-induced preterm mice, and to provide a reference for the prevention and treatment of brain injury. An intraperitoneal injection of lipopolysaccharide in pregnant mice was performed to establish a m...
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| Veröffentlicht in: | Zhongguo dang dai er ke za zhi 2016-05, Vol.18 (5), p.435-439 |
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| creator | Chen, Ru-Juan Xi, Sha Wang, Fan Xiao, Mi Lin, Xiao-Jie Liu, Li |
| description | To investigate the association between long non-coding RNAs (lncRNAs) and brain injury in inflammation-induced preterm mice, and to provide a reference for the prevention and treatment of brain injury.
An intraperitoneal injection of lipopolysaccharide in pregnant mice was performed to establish a model of inflammation-induced preterm mice with brain injury (preterm group). The full-term mice delivered by normal pregnant mice were used as controls (full-term group). The lncRNA chip assay was used to screen out the lncRNAs associated with brain injury in preterm mice. Quantitative real-time PCR was used to validate the lncRNAs identified by the above method.
The preterm and full-term groups showed significant differences in the expression of 1 978 lncRNAs (P |
| doi_str_mv | 10.7499/j.issn.1008-8830.2016.05.012 |
| format | Article |
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An intraperitoneal injection of lipopolysaccharide in pregnant mice was performed to establish a model of inflammation-induced preterm mice with brain injury (preterm group). The full-term mice delivered by normal pregnant mice were used as controls (full-term group). The lncRNA chip assay was used to screen out the lncRNAs associated with brain injury in preterm mice. Quantitative real-time PCR was used to validate the lncRNAs identified by the above method.
The preterm and full-term groups showed significant differences in the expression of 1 978 lncRNAs (P<0.05), consisting of 786 up-regulated lncRNAs and 1 192 down-regulated lncRNAs, and 29 lncRNAs were 1.5 or more times differentially expressed between the two groups. A further analysis was performed for the 10 most differentially expressed lncRNAs, and th</description><identifier>ISSN: 1008-8830</identifier><identifier>DOI: 10.7499/j.issn.1008-8830.2016.05.012</identifier><identifier>PMID: 27165594</identifier><language>chi</language><publisher>China</publisher><subject>Animals ; Brain - metabolism ; Female ; Inflammation - complications ; Inflammation - metabolism ; Mice ; Mice, Inbred BALB C ; Receptors, G-Protein-Coupled - physiology ; RNA, Long Noncoding - analysis ; Signal Transduction - physiology</subject><ispartof>Zhongguo dang dai er ke za zhi, 2016-05, Vol.18 (5), p.435-439</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27165594$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Ru-Juan</creatorcontrib><creatorcontrib>Xi, Sha</creatorcontrib><creatorcontrib>Wang, Fan</creatorcontrib><creatorcontrib>Xiao, Mi</creatorcontrib><creatorcontrib>Lin, Xiao-Jie</creatorcontrib><creatorcontrib>Liu, Li</creatorcontrib><title>Screening and validation of long non-coding RNAs in brain tissue of inflammation-induced preterm mice</title><title>Zhongguo dang dai er ke za zhi</title><addtitle>Zhongguo Dang Dai Er Ke Za Zhi</addtitle><description>To investigate the association between long non-coding RNAs (lncRNAs) and brain injury in inflammation-induced preterm mice, and to provide a reference for the prevention and treatment of brain injury.
An intraperitoneal injection of lipopolysaccharide in pregnant mice was performed to establish a model of inflammation-induced preterm mice with brain injury (preterm group). The full-term mice delivered by normal pregnant mice were used as controls (full-term group). The lncRNA chip assay was used to screen out the lncRNAs associated with brain injury in preterm mice. Quantitative real-time PCR was used to validate the lncRNAs identified by the above method.
The preterm and full-term groups showed significant differences in the expression of 1 978 lncRNAs (P<0.05), consisting of 786 up-regulated lncRNAs and 1 192 down-regulated lncRNAs, and 29 lncRNAs were 1.5 or more times differentially expressed between the two groups. A further analysis was performed for the 10 most differentially expressed lncRNAs, and th</description><subject>Animals</subject><subject>Brain - metabolism</subject><subject>Female</subject><subject>Inflammation - complications</subject><subject>Inflammation - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Receptors, G-Protein-Coupled - physiology</subject><subject>RNA, Long Noncoding - analysis</subject><subject>Signal Transduction - physiology</subject><issn>1008-8830</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEtPwzAQhH0A0VL4C8gHDlwS7DiO7WNV8ZIqkHicIz_WyFXihDhB4t-TQuEyK42-3dEOQpeU5KJU6nqXh5RiTgmRmZSM5AWhVU54TmhxhJb__gKdprQjhMtSsRO0KAStOFflEsGLHQBiiO9YR4c_dROcHkMXcedx08127GJmO7cnnh_XCYeIzaBnHefsCfZciL7Rbfuzl4XoJgsO9wOMMLS4DRbO0LHXTYLzw1yht9ub1819tn26e9ist1lPi2rMrBGEGmMkgOCM28oL5g0xVaFkRRzz8z-6pM5r4ktwCrgooKwUt0KCs4at0NXv3X7oPiZIY92GZKFpdIRuSjUVUnImpOIzenFAJ9OCq_shtHr4qv-qYd9zxGil</recordid><startdate>201605</startdate><enddate>201605</enddate><creator>Chen, Ru-Juan</creator><creator>Xi, Sha</creator><creator>Wang, Fan</creator><creator>Xiao, Mi</creator><creator>Lin, Xiao-Jie</creator><creator>Liu, Li</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201605</creationdate><title>Screening and validation of long non-coding RNAs in brain tissue of inflammation-induced preterm mice</title><author>Chen, Ru-Juan ; Xi, Sha ; Wang, Fan ; Xiao, Mi ; Lin, Xiao-Jie ; Liu, Li</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p126t-cb701bbb8ee7535c6f73fb0b629860d3f008a41dfa0f4ed9e572e4695c78edcb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Brain - metabolism</topic><topic>Female</topic><topic>Inflammation - complications</topic><topic>Inflammation - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Receptors, G-Protein-Coupled - physiology</topic><topic>RNA, Long Noncoding - analysis</topic><topic>Signal Transduction - physiology</topic><toplevel>online_resources</toplevel><creatorcontrib>Chen, Ru-Juan</creatorcontrib><creatorcontrib>Xi, Sha</creatorcontrib><creatorcontrib>Wang, Fan</creatorcontrib><creatorcontrib>Xiao, Mi</creatorcontrib><creatorcontrib>Lin, Xiao-Jie</creatorcontrib><creatorcontrib>Liu, Li</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Zhongguo dang dai er ke za zhi</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Ru-Juan</au><au>Xi, Sha</au><au>Wang, Fan</au><au>Xiao, Mi</au><au>Lin, Xiao-Jie</au><au>Liu, Li</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Screening and validation of long non-coding RNAs in brain tissue of inflammation-induced preterm mice</atitle><jtitle>Zhongguo dang dai er ke za zhi</jtitle><addtitle>Zhongguo Dang Dai Er Ke Za Zhi</addtitle><date>2016-05</date><risdate>2016</risdate><volume>18</volume><issue>5</issue><spage>435</spage><epage>439</epage><pages>435-439</pages><issn>1008-8830</issn><abstract>To investigate the association between long non-coding RNAs (lncRNAs) and brain injury in inflammation-induced preterm mice, and to provide a reference for the prevention and treatment of brain injury.
An intraperitoneal injection of lipopolysaccharide in pregnant mice was performed to establish a model of inflammation-induced preterm mice with brain injury (preterm group). The full-term mice delivered by normal pregnant mice were used as controls (full-term group). The lncRNA chip assay was used to screen out the lncRNAs associated with brain injury in preterm mice. Quantitative real-time PCR was used to validate the lncRNAs identified by the above method.
The preterm and full-term groups showed significant differences in the expression of 1 978 lncRNAs (P<0.05), consisting of 786 up-regulated lncRNAs and 1 192 down-regulated lncRNAs, and 29 lncRNAs were 1.5 or more times differentially expressed between the two groups. A further analysis was performed for the 10 most differentially expressed lncRNAs, and th</abstract><cop>China</cop><pmid>27165594</pmid><doi>10.7499/j.issn.1008-8830.2016.05.012</doi><tpages>5</tpages></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Animals Brain - metabolism Female Inflammation - complications Inflammation - metabolism Mice Mice, Inbred BALB C Receptors, G-Protein-Coupled - physiology RNA, Long Noncoding - analysis Signal Transduction - physiology |
| title | Screening and validation of long non-coding RNAs in brain tissue of inflammation-induced preterm mice |
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