Isolation and characterization of the murine transforming growth factor- beta 2 promoter

Isolation and characterization of the murine transforming growth factor- beta 2 promoter

This report describes the isolation and characterization of the 5' flanking region of the murine transforming growth factor beta-2 (TGF- beta 2) gene. A genomic clone containing the promoter region of the gene was isolated after screening a bacteriophage P1 genomic library. The resulting clone...

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Veröffentlicht in:Gene 2001-05, Vol.270 (1-2), p.201-209
Hauptverfasser: Wilder, P J, Desler, M M, Nowling, T K, Rizzino, A
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TGF-^b2 gene
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creator Wilder, P J
Desler, M M
Nowling, T K
Rizzino, A
description This report describes the isolation and characterization of the 5' flanking region of the murine transforming growth factor beta-2 (TGF- beta 2) gene. A genomic clone containing the promoter region of the gene was isolated after screening a bacteriophage P1 genomic library. The resulting clone was sequenced and compared to promoters for the human and chicken TGF- beta 2 genes. The sequence located near the transcription start site is highly conserved. It includes a TATA box, an E-box, and a largely conserved CRE/ATF site. A series of murine TGF- beta 2 promoter /reporter constructs was generated to identify regulatory regions of the gene. As in the case of the human TGF- beta 2 gene, sequences just upstream of the TATA box, including the CRE/ATF site, actively stimulate the murine TGF- beta 2 promoter. However, unlike the human TGF- beta 2 gene, the 5' flanking region of the murine TGF- beta 2 gene contains a long alternating purine/pyrimidine repeat that unexpectedly exerts a strong positive effect on its promoter. This is of particular interest since alternating purine/pyrimidine repeats in other promoters have been observed to be inhibitory.
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title Isolation and characterization of the murine transforming growth factor- beta 2 promoter
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