Identification of anti‐biofilm components in Withania somnifera and their effect on virulence of Streptococcus mutans biofilms
Aims The aim of this study was to identify components of the Withania somnifera that could show anti‐virulence activity against Streptococcus mutans biofilms. Methods and Results The anti‐acidogenic activity of fractions separated from W. somnifera was compared, and then the most active anti‐acidoge...
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| Veröffentlicht in: | Journal of applied microbiology 2015-08, Vol.119 (2), p.571-581 |
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| creator | Pandit, S. Cai, J.N. Song, K.Y. Jeon, J.G. |
| description | Aims
The aim of this study was to identify components of the Withania somnifera that could show anti‐virulence activity against Streptococcus mutans biofilms.
Methods and Results
The anti‐acidogenic activity of fractions separated from W. somnifera was compared, and then the most active anti‐acidogenic fraction was chemically characterized using gas chromatography‐mass spectroscopy. The effect of the identified components on the acidogenicity, aciduricity and extracellular polymeric substances (EPS) formation of S. mutans UA159 biofilms was evaluated. The change in accumulation and acidogenicity of S. mutans UA159 biofilms by periodic treatments (10 min per treatment) with the identified components was also investigated. Of the fractions, n‐hexane fraction showed the strongest anti‐acidogenic activity and was mainly composed of palmitic, linoleic and oleic acids. Of the identified components, linoleic and oleic acids strongly affected the acid production rate, F‐ATPase activity and EPS formation of the biofilms. Periodic treatment with linoleic and oleic acids during biofilm formation also inhibited the biofilm accumulation and acid production rate of the biofilms without killing the biofilm bacteria.
Conclusions
These results suggest that linoleic and oleic acids may be effective agents for restraining virulence of S. mutans biofilms.
Significance and Impact of the Study
Linoleic and oleic acids may be promising agents for controlling virulence of cariogenic biofilms and subsequent dental caries formation. |
| doi_str_mv | 10.1111/jam.12851 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1787984469</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1697756356</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3861-28c856a6af1e4745868fef26f19bce58f3277eb16b9c07f6f56ccf47967b10443</originalsourceid><addsrcrecordid>eNqF0cFqFDEYwPEgiq2rB19AAl70MG2SmXzJHEuxWql4UPE4ZLJfaJaZZE0ySm99hD6jT2K6u3oQxBBIAj_-ED5CnnN2wus63Zj5hAst-QNyzFuQjQAlHu7uXSOZEkfkSc4bxnjLJDwmR0L2CrgQx-T2co2heOetKT4GGh019f3z9m700flppjbO2xgqytQH-tWXaxO8oTnOwTtMpvo1LdfoE0Xn0BZaM999WiYMFu-Dn0rCbYk2WrtkOi_FhEwP-fyUPHJmyvjscK7Il4s3n8_fNVcf316en101ttXAG6GtlmDAOI6d6qQG7dAJcLwfLUrtWqEUjhzG3jLlwEmw1nWqBzVy1nXtirzad7cpflswl2H22eI0mYBxyQNXWvW666D_P4VeKQlt3Svy8i-6iUsK9SM7xZVkwKp6vVc2xZwTumGb_GzSzcDZcD_BoU5w2E2w2heH4jLOuP4jf4-sgtM9-OEnvPl3aXh_9mGf_AVD4acC</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1697175060</pqid></control><display><type>article</type><title>Identification of anti‐biofilm components in Withania somnifera and their effect on virulence of Streptococcus mutans biofilms</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Pandit, S. ; Cai, J.N. ; Song, K.Y. ; Jeon, J.G.</creator><creatorcontrib>Pandit, S. ; Cai, J.N. ; Song, K.Y. ; Jeon, J.G.</creatorcontrib><description>Aims
The aim of this study was to identify components of the Withania somnifera that could show anti‐virulence activity against Streptococcus mutans biofilms.
Methods and Results
The anti‐acidogenic activity of fractions separated from W. somnifera was compared, and then the most active anti‐acidogenic fraction was chemically characterized using gas chromatography‐mass spectroscopy. The effect of the identified components on the acidogenicity, aciduricity and extracellular polymeric substances (EPS) formation of S. mutans UA159 biofilms was evaluated. The change in accumulation and acidogenicity of S. mutans UA159 biofilms by periodic treatments (10 min per treatment) with the identified components was also investigated. Of the fractions, n‐hexane fraction showed the strongest anti‐acidogenic activity and was mainly composed of palmitic, linoleic and oleic acids. Of the identified components, linoleic and oleic acids strongly affected the acid production rate, F‐ATPase activity and EPS formation of the biofilms. Periodic treatment with linoleic and oleic acids during biofilm formation also inhibited the biofilm accumulation and acid production rate of the biofilms without killing the biofilm bacteria.
Conclusions
These results suggest that linoleic and oleic acids may be effective agents for restraining virulence of S. mutans biofilms.
Significance and Impact of the Study
Linoleic and oleic acids may be promising agents for controlling virulence of cariogenic biofilms and subsequent dental caries formation.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.12851</identifier><identifier>PMID: 25976122</identifier><identifier>CODEN: JAMIFK</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>acidogenicity ; aciduricity ; Bacteriology ; Biofilms ; Biofilms - drug effects ; Dental caries ; Dental Caries - microbiology ; Gas Chromatography-Mass Spectrometry ; Humans ; linoleic acid ; Microbiology ; Plant Extracts - chemistry ; Plant Extracts - pharmacology ; Streptococcal Infections - microbiology ; Streptococcus mutans ; Streptococcus mutans - drug effects ; Streptococcus mutans - pathogenicity ; Streptococcus mutans - physiology ; Streptococcus mutans biofilm ; Virology ; Virulence - drug effects ; Withania - chemistry ; Withania somnifera</subject><ispartof>Journal of applied microbiology, 2015-08, Vol.119 (2), p.571-581</ispartof><rights>2015 The Society for Applied Microbiology</rights><rights>2015 The Society for Applied Microbiology.</rights><rights>Copyright © 2015 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3861-28c856a6af1e4745868fef26f19bce58f3277eb16b9c07f6f56ccf47967b10443</citedby><cites>FETCH-LOGICAL-c3861-28c856a6af1e4745868fef26f19bce58f3277eb16b9c07f6f56ccf47967b10443</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjam.12851$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjam.12851$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25976122$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pandit, S.</creatorcontrib><creatorcontrib>Cai, J.N.</creatorcontrib><creatorcontrib>Song, K.Y.</creatorcontrib><creatorcontrib>Jeon, J.G.</creatorcontrib><title>Identification of anti‐biofilm components in Withania somnifera and their effect on virulence of Streptococcus mutans biofilms</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims
The aim of this study was to identify components of the Withania somnifera that could show anti‐virulence activity against Streptococcus mutans biofilms.
Methods and Results
The anti‐acidogenic activity of fractions separated from W. somnifera was compared, and then the most active anti‐acidogenic fraction was chemically characterized using gas chromatography‐mass spectroscopy. The effect of the identified components on the acidogenicity, aciduricity and extracellular polymeric substances (EPS) formation of S. mutans UA159 biofilms was evaluated. The change in accumulation and acidogenicity of S. mutans UA159 biofilms by periodic treatments (10 min per treatment) with the identified components was also investigated. Of the fractions, n‐hexane fraction showed the strongest anti‐acidogenic activity and was mainly composed of palmitic, linoleic and oleic acids. Of the identified components, linoleic and oleic acids strongly affected the acid production rate, F‐ATPase activity and EPS formation of the biofilms. Periodic treatment with linoleic and oleic acids during biofilm formation also inhibited the biofilm accumulation and acid production rate of the biofilms without killing the biofilm bacteria.
Conclusions
These results suggest that linoleic and oleic acids may be effective agents for restraining virulence of S. mutans biofilms.
Significance and Impact of the Study
Linoleic and oleic acids may be promising agents for controlling virulence of cariogenic biofilms and subsequent dental caries formation.</description><subject>acidogenicity</subject><subject>aciduricity</subject><subject>Bacteriology</subject><subject>Biofilms</subject><subject>Biofilms - drug effects</subject><subject>Dental caries</subject><subject>Dental Caries - microbiology</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>Humans</subject><subject>linoleic acid</subject><subject>Microbiology</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - pharmacology</subject><subject>Streptococcal Infections - microbiology</subject><subject>Streptococcus mutans</subject><subject>Streptococcus mutans - drug effects</subject><subject>Streptococcus mutans - pathogenicity</subject><subject>Streptococcus mutans - physiology</subject><subject>Streptococcus mutans biofilm</subject><subject>Virology</subject><subject>Virulence - drug effects</subject><subject>Withania - chemistry</subject><subject>Withania somnifera</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0cFqFDEYwPEgiq2rB19AAl70MG2SmXzJHEuxWql4UPE4ZLJfaJaZZE0ySm99hD6jT2K6u3oQxBBIAj_-ED5CnnN2wus63Zj5hAst-QNyzFuQjQAlHu7uXSOZEkfkSc4bxnjLJDwmR0L2CrgQx-T2co2heOetKT4GGh019f3z9m700flppjbO2xgqytQH-tWXaxO8oTnOwTtMpvo1LdfoE0Xn0BZaM999WiYMFu-Dn0rCbYk2WrtkOi_FhEwP-fyUPHJmyvjscK7Il4s3n8_fNVcf316en101ttXAG6GtlmDAOI6d6qQG7dAJcLwfLUrtWqEUjhzG3jLlwEmw1nWqBzVy1nXtirzad7cpflswl2H22eI0mYBxyQNXWvW666D_P4VeKQlt3Svy8i-6iUsK9SM7xZVkwKp6vVc2xZwTumGb_GzSzcDZcD_BoU5w2E2w2heH4jLOuP4jf4-sgtM9-OEnvPl3aXh_9mGf_AVD4acC</recordid><startdate>201508</startdate><enddate>201508</enddate><creator>Pandit, S.</creator><creator>Cai, J.N.</creator><creator>Song, K.Y.</creator><creator>Jeon, J.G.</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7ST</scope><scope>SOI</scope></search><sort><creationdate>201508</creationdate><title>Identification of anti‐biofilm components in Withania somnifera and their effect on virulence of Streptococcus mutans biofilms</title><author>Pandit, S. ; Cai, J.N. ; Song, K.Y. ; Jeon, J.G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3861-28c856a6af1e4745868fef26f19bce58f3277eb16b9c07f6f56ccf47967b10443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>acidogenicity</topic><topic>aciduricity</topic><topic>Bacteriology</topic><topic>Biofilms</topic><topic>Biofilms - drug effects</topic><topic>Dental caries</topic><topic>Dental Caries - microbiology</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>Humans</topic><topic>linoleic acid</topic><topic>Microbiology</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - pharmacology</topic><topic>Streptococcal Infections - microbiology</topic><topic>Streptococcus mutans</topic><topic>Streptococcus mutans - drug effects</topic><topic>Streptococcus mutans - pathogenicity</topic><topic>Streptococcus mutans - physiology</topic><topic>Streptococcus mutans biofilm</topic><topic>Virology</topic><topic>Virulence - drug effects</topic><topic>Withania - chemistry</topic><topic>Withania somnifera</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pandit, S.</creatorcontrib><creatorcontrib>Cai, J.N.</creatorcontrib><creatorcontrib>Song, K.Y.</creatorcontrib><creatorcontrib>Jeon, J.G.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Environment Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pandit, S.</au><au>Cai, J.N.</au><au>Song, K.Y.</au><au>Jeon, J.G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of anti‐biofilm components in Withania somnifera and their effect on virulence of Streptococcus mutans biofilms</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2015-08</date><risdate>2015</risdate><volume>119</volume><issue>2</issue><spage>571</spage><epage>581</epage><pages>571-581</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><coden>JAMIFK</coden><abstract>Aims
The aim of this study was to identify components of the Withania somnifera that could show anti‐virulence activity against Streptococcus mutans biofilms.
Methods and Results
The anti‐acidogenic activity of fractions separated from W. somnifera was compared, and then the most active anti‐acidogenic fraction was chemically characterized using gas chromatography‐mass spectroscopy. The effect of the identified components on the acidogenicity, aciduricity and extracellular polymeric substances (EPS) formation of S. mutans UA159 biofilms was evaluated. The change in accumulation and acidogenicity of S. mutans UA159 biofilms by periodic treatments (10 min per treatment) with the identified components was also investigated. Of the fractions, n‐hexane fraction showed the strongest anti‐acidogenic activity and was mainly composed of palmitic, linoleic and oleic acids. Of the identified components, linoleic and oleic acids strongly affected the acid production rate, F‐ATPase activity and EPS formation of the biofilms. Periodic treatment with linoleic and oleic acids during biofilm formation also inhibited the biofilm accumulation and acid production rate of the biofilms without killing the biofilm bacteria.
Conclusions
These results suggest that linoleic and oleic acids may be effective agents for restraining virulence of S. mutans biofilms.
Significance and Impact of the Study
Linoleic and oleic acids may be promising agents for controlling virulence of cariogenic biofilms and subsequent dental caries formation.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>25976122</pmid><doi>10.1111/jam.12851</doi><tpages>11</tpages></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | acidogenicity aciduricity Bacteriology Biofilms Biofilms - drug effects Dental caries Dental Caries - microbiology Gas Chromatography-Mass Spectrometry Humans linoleic acid Microbiology Plant Extracts - chemistry Plant Extracts - pharmacology Streptococcal Infections - microbiology Streptococcus mutans Streptococcus mutans - drug effects Streptococcus mutans - pathogenicity Streptococcus mutans - physiology Streptococcus mutans biofilm Virology Virulence - drug effects Withania - chemistry Withania somnifera |
| title | Identification of anti‐biofilm components in Withania somnifera and their effect on virulence of Streptococcus mutans biofilms |
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