Co‐overexpression of lmbW and metK led to increased lincomycin A production and decreased byproduct lincomycin B content in an industrial strain of Streptomyces lincolnensis
Aims To improve lincomycin A production and decrease the content of byproduct lincomycin B in an industrial lincomycin‐producing strain. Methods and Results The in silico analysis indicated that LmbW could be involved in propylproline biosynthesis of lincomyin A. In this study, we constructed an lmb...
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creator | Pang, A.‐P. Du, L. Lin, C.‐Y. Qiao, J. Zhao, G.‐R. |
description | Aims
To improve lincomycin A production and decrease the content of byproduct lincomycin B in an industrial lincomycin‐producing strain.
Methods and Results
The in silico analysis indicated that LmbW could be involved in propylproline biosynthesis of lincomyin A. In this study, we constructed an lmbW deletion mutant and found that the mutant lost the ability to produce lincomycin A, but increased the accumulation of lincomycin B. The loss of lincomycin A production can be restored by complementing the mutant with the expression of lmbW gene. When lmbW and metK (encoding S‐adenosylmethionine synthetase) was co‐overexpressed, lincomycin A titre was 1744·6 mg l−1, a 35·83% improvement over the original strain. Meanwhile, the content of lincomycin B was reduced to 4·41%, a remarkable decrease of 34·76%, compared to that of the original strain.
Conclusions
lmbW encodes a C‐methyltransferase involved in the biosynthesis of lincomycin A but not lincomycin B. Co‐overexpression of lmbW and metK improved lincomycin A production and decreased the content of lincomycin B.
Significance and Impact of the Study
The engineered Streptomyces lincolnensis strain shows promising application in the fermentation production of lincomycin A, which may help cut production costs and simplify downstream separation processes. |
doi_str_mv | 10.1111/jam.12919 |
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fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1787981275</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3812949171</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4219-cc56b25d4ab3ab48e50d28a449443d1f116a95e7e5c0557c9843acc7210a0c903</originalsourceid><addsrcrecordid>eNqNkc2KFDEUhYMozji68AUk4EYXNZPfSmXZNv6PuFBxWaRSt6GaVNImKbV3PsK8ybyTT2Kqq0dEEMwi94Z851wuB6GHlJzTci62ZjynTFN9C51SXsuK1YrdPvSikkSxE3QvpS0hlBNZ30UnrGaiEZqcout1-PnjKnyFCN93EVIagsdhg93YfcbG93iE_BY76HEOePA2gknl4Uobxr0dPF7hXQz9ZPOsnBU93FDd_vj1J_8M2-Az-FzsCl_ufko5DsbhUsxwGP8hR9jlWQFpETsPPg3pPrqzMS7Bg2M9Q59ePP-4flVdvn_5er26rKxgVFfWyrpjshem46YTDUjSs8YIoYXgPd1QWhstQYG0REpldSO4sVYxSgyxmvAz9GTxLRt8mSDldhySBeeMhzCllqpG6YYyJf8DpbKWmite0Md_odswRV8WmSmuZa20LtTThbIxpBRh0-7iMJq4bylp58DbEnh7CLywj46OUzdC_5u8SbgAFwvwbXCw_7dT-2b1brH8BRx4uEU</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1713956799</pqid></control><display><type>article</type><title>Co‐overexpression of lmbW and metK led to increased lincomycin A production and decreased byproduct lincomycin B content in an industrial strain of Streptomyces lincolnensis</title><source>MEDLINE</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Wiley Online Library All Journals</source><creator>Pang, A.‐P. ; Du, L. ; Lin, C.‐Y. ; Qiao, J. ; Zhao, G.‐R.</creator><creatorcontrib>Pang, A.‐P. ; Du, L. ; Lin, C.‐Y. ; Qiao, J. ; Zhao, G.‐R.</creatorcontrib><description>Aims
To improve lincomycin A production and decrease the content of byproduct lincomycin B in an industrial lincomycin‐producing strain.
Methods and Results
The in silico analysis indicated that LmbW could be involved in propylproline biosynthesis of lincomyin A. In this study, we constructed an lmbW deletion mutant and found that the mutant lost the ability to produce lincomycin A, but increased the accumulation of lincomycin B. The loss of lincomycin A production can be restored by complementing the mutant with the expression of lmbW gene. When lmbW and metK (encoding S‐adenosylmethionine synthetase) was co‐overexpressed, lincomycin A titre was 1744·6 mg l−1, a 35·83% improvement over the original strain. Meanwhile, the content of lincomycin B was reduced to 4·41%, a remarkable decrease of 34·76%, compared to that of the original strain.
Conclusions
lmbW encodes a C‐methyltransferase involved in the biosynthesis of lincomycin A but not lincomycin B. Co‐overexpression of lmbW and metK improved lincomycin A production and decreased the content of lincomycin B.
Significance and Impact of the Study
The engineered Streptomyces lincolnensis strain shows promising application in the fermentation production of lincomycin A, which may help cut production costs and simplify downstream separation processes.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.12919</identifier><identifier>PMID: 26248490</identifier><identifier>CODEN: JAMIFK</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Bioengineering ; Biosynthesis ; Biotechnology ; Fermentation ; lincomycin ; Lincomycin - biosynthesis ; Lincosamides - biosynthesis ; lmbW ; metabolic engineering ; Methionine Adenosyltransferase - metabolism ; methyltransferase ; Microbiology ; Molecular Sequence Data ; Streptomyces - genetics ; Streptomyces - metabolism ; Streptomyces lincolnensis ; synthetic biology</subject><ispartof>Journal of applied microbiology, 2015-10, Vol.119 (4), p.1064-1074</ispartof><rights>2015 The Society for Applied Microbiology</rights><rights>2015 The Society for Applied Microbiology.</rights><rights>Copyright © 2015 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4219-cc56b25d4ab3ab48e50d28a449443d1f116a95e7e5c0557c9843acc7210a0c903</citedby><cites>FETCH-LOGICAL-c4219-cc56b25d4ab3ab48e50d28a449443d1f116a95e7e5c0557c9843acc7210a0c903</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjam.12919$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjam.12919$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26248490$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pang, A.‐P.</creatorcontrib><creatorcontrib>Du, L.</creatorcontrib><creatorcontrib>Lin, C.‐Y.</creatorcontrib><creatorcontrib>Qiao, J.</creatorcontrib><creatorcontrib>Zhao, G.‐R.</creatorcontrib><title>Co‐overexpression of lmbW and metK led to increased lincomycin A production and decreased byproduct lincomycin B content in an industrial strain of Streptomyces lincolnensis</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims
To improve lincomycin A production and decrease the content of byproduct lincomycin B in an industrial lincomycin‐producing strain.
Methods and Results
The in silico analysis indicated that LmbW could be involved in propylproline biosynthesis of lincomyin A. In this study, we constructed an lmbW deletion mutant and found that the mutant lost the ability to produce lincomycin A, but increased the accumulation of lincomycin B. The loss of lincomycin A production can be restored by complementing the mutant with the expression of lmbW gene. When lmbW and metK (encoding S‐adenosylmethionine synthetase) was co‐overexpressed, lincomycin A titre was 1744·6 mg l−1, a 35·83% improvement over the original strain. Meanwhile, the content of lincomycin B was reduced to 4·41%, a remarkable decrease of 34·76%, compared to that of the original strain.
Conclusions
lmbW encodes a C‐methyltransferase involved in the biosynthesis of lincomycin A but not lincomycin B. Co‐overexpression of lmbW and metK improved lincomycin A production and decreased the content of lincomycin B.
Significance and Impact of the Study
The engineered Streptomyces lincolnensis strain shows promising application in the fermentation production of lincomycin A, which may help cut production costs and simplify downstream separation processes.</description><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bioengineering</subject><subject>Biosynthesis</subject><subject>Biotechnology</subject><subject>Fermentation</subject><subject>lincomycin</subject><subject>Lincomycin - biosynthesis</subject><subject>Lincosamides - biosynthesis</subject><subject>lmbW</subject><subject>metabolic engineering</subject><subject>Methionine Adenosyltransferase - metabolism</subject><subject>methyltransferase</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Streptomyces - genetics</subject><subject>Streptomyces - metabolism</subject><subject>Streptomyces lincolnensis</subject><subject>synthetic biology</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc2KFDEUhYMozji68AUk4EYXNZPfSmXZNv6PuFBxWaRSt6GaVNImKbV3PsK8ybyTT2Kqq0dEEMwi94Z851wuB6GHlJzTci62ZjynTFN9C51SXsuK1YrdPvSikkSxE3QvpS0hlBNZ30UnrGaiEZqcout1-PnjKnyFCN93EVIagsdhg93YfcbG93iE_BY76HEOePA2gknl4Uobxr0dPF7hXQz9ZPOsnBU93FDd_vj1J_8M2-Az-FzsCl_ufko5DsbhUsxwGP8hR9jlWQFpETsPPg3pPrqzMS7Bg2M9Q59ePP-4flVdvn_5er26rKxgVFfWyrpjshem46YTDUjSs8YIoYXgPd1QWhstQYG0REpldSO4sVYxSgyxmvAz9GTxLRt8mSDldhySBeeMhzCllqpG6YYyJf8DpbKWmite0Md_odswRV8WmSmuZa20LtTThbIxpBRh0-7iMJq4bylp58DbEnh7CLywj46OUzdC_5u8SbgAFwvwbXCw_7dT-2b1brH8BRx4uEU</recordid><startdate>201510</startdate><enddate>201510</enddate><creator>Pang, A.‐P.</creator><creator>Du, L.</creator><creator>Lin, C.‐Y.</creator><creator>Qiao, J.</creator><creator>Zhao, G.‐R.</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7ST</scope><scope>SOI</scope></search><sort><creationdate>201510</creationdate><title>Co‐overexpression of lmbW and metK led to increased lincomycin A production and decreased byproduct lincomycin B content in an industrial strain of Streptomyces lincolnensis</title><author>Pang, A.‐P. ; Du, L. ; Lin, C.‐Y. ; Qiao, J. ; Zhao, G.‐R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4219-cc56b25d4ab3ab48e50d28a449443d1f116a95e7e5c0557c9843acc7210a0c903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bioengineering</topic><topic>Biosynthesis</topic><topic>Biotechnology</topic><topic>Fermentation</topic><topic>lincomycin</topic><topic>Lincomycin - biosynthesis</topic><topic>Lincosamides - biosynthesis</topic><topic>lmbW</topic><topic>metabolic engineering</topic><topic>Methionine Adenosyltransferase - metabolism</topic><topic>methyltransferase</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Streptomyces - genetics</topic><topic>Streptomyces - metabolism</topic><topic>Streptomyces lincolnensis</topic><topic>synthetic biology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pang, A.‐P.</creatorcontrib><creatorcontrib>Du, L.</creatorcontrib><creatorcontrib>Lin, C.‐Y.</creatorcontrib><creatorcontrib>Qiao, J.</creatorcontrib><creatorcontrib>Zhao, G.‐R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Environment Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pang, A.‐P.</au><au>Du, L.</au><au>Lin, C.‐Y.</au><au>Qiao, J.</au><au>Zhao, G.‐R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Co‐overexpression of lmbW and metK led to increased lincomycin A production and decreased byproduct lincomycin B content in an industrial strain of Streptomyces lincolnensis</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2015-10</date><risdate>2015</risdate><volume>119</volume><issue>4</issue><spage>1064</spage><epage>1074</epage><pages>1064-1074</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><coden>JAMIFK</coden><abstract>Aims
To improve lincomycin A production and decrease the content of byproduct lincomycin B in an industrial lincomycin‐producing strain.
Methods and Results
The in silico analysis indicated that LmbW could be involved in propylproline biosynthesis of lincomyin A. In this study, we constructed an lmbW deletion mutant and found that the mutant lost the ability to produce lincomycin A, but increased the accumulation of lincomycin B. The loss of lincomycin A production can be restored by complementing the mutant with the expression of lmbW gene. When lmbW and metK (encoding S‐adenosylmethionine synthetase) was co‐overexpressed, lincomycin A titre was 1744·6 mg l−1, a 35·83% improvement over the original strain. Meanwhile, the content of lincomycin B was reduced to 4·41%, a remarkable decrease of 34·76%, compared to that of the original strain.
Conclusions
lmbW encodes a C‐methyltransferase involved in the biosynthesis of lincomycin A but not lincomycin B. Co‐overexpression of lmbW and metK improved lincomycin A production and decreased the content of lincomycin B.
Significance and Impact of the Study
The engineered Streptomyces lincolnensis strain shows promising application in the fermentation production of lincomycin A, which may help cut production costs and simplify downstream separation processes.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>26248490</pmid><doi>10.1111/jam.12919</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Bacterial Proteins - genetics Bacterial Proteins - metabolism Bioengineering Biosynthesis Biotechnology Fermentation lincomycin Lincomycin - biosynthesis Lincosamides - biosynthesis lmbW metabolic engineering Methionine Adenosyltransferase - metabolism methyltransferase Microbiology Molecular Sequence Data Streptomyces - genetics Streptomyces - metabolism Streptomyces lincolnensis synthetic biology |
title | Co‐overexpression of lmbW and metK led to increased lincomycin A production and decreased byproduct lincomycin B content in an industrial strain of Streptomyces lincolnensis |
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