Resistance to antimicrobial agents among enterococci isolated from fecal samples of wild marine species in the southern coast of Brazil
The purpose of this study was to evaluate species distribution, antimicrobial resistance profiles, and presence of resistance genes in enterococci isolated from fecal samples of wild marine species, including seabirds (n=12), sea turtles (n=8), and mammals (n=3) found alive or dead in southern coast...
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| Veröffentlicht in: | Marine pollution bulletin 2016-04, Vol.105 (1), p.51-57 |
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| creator | Prichula, Janira Pereira, Rebeca Inhoque Wachholz, Guilherme Raffo Cardoso, Leonardo Almansa Tolfo, Neidimar Cezar Correa Santestevan, Naiara Aguiar Medeiros, Aline Weber Tavares, Maurício Frazzon, Jeverson d'Azevedo, Pedro Alves Frazzon, Ana Paula Guedes |
| description | The purpose of this study was to evaluate species distribution, antimicrobial resistance profiles, and presence of resistance genes in enterococci isolated from fecal samples of wild marine species, including seabirds (n=12), sea turtles (n=8), and mammals (n=3) found alive or dead in southern coast of Brazil. Enterococci were classified based on phenotypic and genotypic characteristics, tested for antibiotic susceptibility, and the presence of tet(S), tet(M), tet(L), mrsC, and erm(B) genes by PCR. Enterococcus faecalis and Enterococcus faecium were the most common species. Single (37.09%), double (25.80%), and multiple (16.12%) antibiotic resistance patterns were observed. Resistance to rifampicin occurred most frequently. The msrC, tet(M), and/or tet(L) genes were detected in 60.15%, 73.07%, and 23.07% of the resistant strains, respectively. In conclusion, the presence of antibiotic resistant strains in these species could be related to food web interactions and aquatic pollutants or linked to environmental resistome.
The paper of Prichula et al. describes the antimicrobial resistance profiles in different species of enterococci isolated from fecal samples of wild marine species, pointing to the importance of these wild species in studies of antibiotic resistance in the marine ecosystem. [Display omitted]
•Very few studies have examined the antimicrobial resistance in enterococci in fecal samples of wild marine species.•This study report the species distribution and occurrence of resistant-enterococci in feces of wild marine species.•Antibiotic resistance in enterococci isolated from these animals is an important gap that should be addressed.•Antibiotic resistant strains could be related to food web interactions, aquatic pollutants or environmental resistome. |
| doi_str_mv | 10.1016/j.marpolbul.2016.02.071 |
| format | Article |
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The paper of Prichula et al. describes the antimicrobial resistance profiles in different species of enterococci isolated from fecal samples of wild marine species, pointing to the importance of these wild species in studies of antibiotic resistance in the marine ecosystem. [Display omitted]
•Very few studies have examined the antimicrobial resistance in enterococci in fecal samples of wild marine species.•This study report the species distribution and occurrence of resistant-enterococci in feces of wild marine species.•Antibiotic resistance in enterococci isolated from these animals is an important gap that should be addressed.•Antibiotic resistant strains could be related to food web interactions, aquatic pollutants or environmental resistome.</description><identifier>ISSN: 0025-326X</identifier><identifier>EISSN: 1879-3363</identifier><identifier>DOI: 10.1016/j.marpolbul.2016.02.071</identifier><identifier>PMID: 26952995</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Anti-Bacterial Agents - pharmacology ; antibiotic resistance ; Aquatic Organisms - microbiology ; biogeography ; Brazil ; coasts ; Drug Resistance, Bacterial - genetics ; Drug Resistance, Microbial ; Enterococci resistant ; Enterococcus - isolation & purification ; Enterococcus faecalis ; Enterococcus faecalis - genetics ; Enterococcus faecium ; Enterococcus faecium - genetics ; Environmental Monitoring ; feces ; Feces - microbiology ; food webs ; genes ; mammals ; Marine ; Microbial Sensitivity Tests ; phenotype ; pollutants ; Polymerase Chain Reaction ; Resistance genes ; rifampicin ; sea turtles ; seabirds ; water pollution ; Wild marine species</subject><ispartof>Marine pollution bulletin, 2016-04, Vol.105 (1), p.51-57</ispartof><rights>2016 Elsevier Ltd</rights><rights>Copyright © 2016 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c428t-c5aa5efd7261502b7e3c1257d2a6d4313d787f18d589682cb3a599e928716e333</citedby><cites>FETCH-LOGICAL-c428t-c5aa5efd7261502b7e3c1257d2a6d4313d787f18d589682cb3a599e928716e333</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0025326X16301230$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26952995$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Prichula, Janira</creatorcontrib><creatorcontrib>Pereira, Rebeca Inhoque</creatorcontrib><creatorcontrib>Wachholz, Guilherme Raffo</creatorcontrib><creatorcontrib>Cardoso, Leonardo Almansa</creatorcontrib><creatorcontrib>Tolfo, Neidimar Cezar Correa</creatorcontrib><creatorcontrib>Santestevan, Naiara Aguiar</creatorcontrib><creatorcontrib>Medeiros, Aline Weber</creatorcontrib><creatorcontrib>Tavares, Maurício</creatorcontrib><creatorcontrib>Frazzon, Jeverson</creatorcontrib><creatorcontrib>d'Azevedo, Pedro Alves</creatorcontrib><creatorcontrib>Frazzon, Ana Paula Guedes</creatorcontrib><title>Resistance to antimicrobial agents among enterococci isolated from fecal samples of wild marine species in the southern coast of Brazil</title><title>Marine pollution bulletin</title><addtitle>Mar Pollut Bull</addtitle><description>The purpose of this study was to evaluate species distribution, antimicrobial resistance profiles, and presence of resistance genes in enterococci isolated from fecal samples of wild marine species, including seabirds (n=12), sea turtles (n=8), and mammals (n=3) found alive or dead in southern coast of Brazil. Enterococci were classified based on phenotypic and genotypic characteristics, tested for antibiotic susceptibility, and the presence of tet(S), tet(M), tet(L), mrsC, and erm(B) genes by PCR. Enterococcus faecalis and Enterococcus faecium were the most common species. Single (37.09%), double (25.80%), and multiple (16.12%) antibiotic resistance patterns were observed. Resistance to rifampicin occurred most frequently. The msrC, tet(M), and/or tet(L) genes were detected in 60.15%, 73.07%, and 23.07% of the resistant strains, respectively. In conclusion, the presence of antibiotic resistant strains in these species could be related to food web interactions and aquatic pollutants or linked to environmental resistome.
The paper of Prichula et al. describes the antimicrobial resistance profiles in different species of enterococci isolated from fecal samples of wild marine species, pointing to the importance of these wild species in studies of antibiotic resistance in the marine ecosystem. [Display omitted]
•Very few studies have examined the antimicrobial resistance in enterococci in fecal samples of wild marine species.•This study report the species distribution and occurrence of resistant-enterococci in feces of wild marine species.•Antibiotic resistance in enterococci isolated from these animals is an important gap that should be addressed.•Antibiotic resistant strains could be related to food web interactions, aquatic pollutants or environmental resistome.</description><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>antibiotic resistance</subject><subject>Aquatic Organisms - microbiology</subject><subject>biogeography</subject><subject>Brazil</subject><subject>coasts</subject><subject>Drug Resistance, Bacterial - genetics</subject><subject>Drug Resistance, Microbial</subject><subject>Enterococci resistant</subject><subject>Enterococcus - isolation & purification</subject><subject>Enterococcus faecalis</subject><subject>Enterococcus faecalis - genetics</subject><subject>Enterococcus faecium</subject><subject>Enterococcus faecium - genetics</subject><subject>Environmental Monitoring</subject><subject>feces</subject><subject>Feces - microbiology</subject><subject>food webs</subject><subject>genes</subject><subject>mammals</subject><subject>Marine</subject><subject>Microbial Sensitivity Tests</subject><subject>phenotype</subject><subject>pollutants</subject><subject>Polymerase Chain Reaction</subject><subject>Resistance genes</subject><subject>rifampicin</subject><subject>sea turtles</subject><subject>seabirds</subject><subject>water pollution</subject><subject>Wild marine species</subject><issn>0025-326X</issn><issn>1879-3363</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1TAQhS0EopfCK1Av2ST4J4ntZamAIlVCAiqxsxx7cvGVE19sBwQvwGvX0S3dwmrs0XdmRucgdEFJSwkdXh_a2aRjDOMaWlYbLWEtEfQR2lEpVMP5wB-jHSGsbzgbvp6hZzkfCCGCCfoUnbFB9Uypfof-fILsczGLBVwiNkvxs7cpjt4EbPawlIzNHJc9rk9I0UZrPfY5BlPA4SnFGU9gK5zNfAyQcZzwTx8crgf6BXA-gvW17RdcvtVvXGtJC7bR5LLBb5L57cNz9GQyIcOL-3qObt-9_XJ13dx8fP_h6vKmsR2TpbG9MT1MTrCB9oSNArilrBeOmcF1nHInpJiodL1Ug2R25KZXChSTgg7AOT9Hr05zjyl-XyEXPftsIQSzQFyzplWvJCVS_g_adQNRapsqTmh1LucEkz4mXw34pSnRW2D6oB8C01tgmjBdA6vKl_dL1nEG96D7m1AFLk7AZKI2--Szvv28TSCEqq6WSlyeCKi-_fCQdK6O10CdT2CLdtH_84w7br-2aQ</recordid><startdate>20160415</startdate><enddate>20160415</enddate><creator>Prichula, Janira</creator><creator>Pereira, Rebeca Inhoque</creator><creator>Wachholz, Guilherme Raffo</creator><creator>Cardoso, Leonardo Almansa</creator><creator>Tolfo, Neidimar Cezar Correa</creator><creator>Santestevan, Naiara Aguiar</creator><creator>Medeiros, Aline Weber</creator><creator>Tavares, Maurício</creator><creator>Frazzon, Jeverson</creator><creator>d'Azevedo, Pedro Alves</creator><creator>Frazzon, Ana Paula Guedes</creator><general>Elsevier Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7ST</scope><scope>7T7</scope><scope>7TN</scope><scope>7TV</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H97</scope><scope>L.G</scope><scope>P64</scope><scope>SOI</scope></search><sort><creationdate>20160415</creationdate><title>Resistance to antimicrobial agents among enterococci isolated from fecal samples of wild marine species in the southern coast of Brazil</title><author>Prichula, Janira ; 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Enterococci were classified based on phenotypic and genotypic characteristics, tested for antibiotic susceptibility, and the presence of tet(S), tet(M), tet(L), mrsC, and erm(B) genes by PCR. Enterococcus faecalis and Enterococcus faecium were the most common species. Single (37.09%), double (25.80%), and multiple (16.12%) antibiotic resistance patterns were observed. Resistance to rifampicin occurred most frequently. The msrC, tet(M), and/or tet(L) genes were detected in 60.15%, 73.07%, and 23.07% of the resistant strains, respectively. In conclusion, the presence of antibiotic resistant strains in these species could be related to food web interactions and aquatic pollutants or linked to environmental resistome.
The paper of Prichula et al. describes the antimicrobial resistance profiles in different species of enterococci isolated from fecal samples of wild marine species, pointing to the importance of these wild species in studies of antibiotic resistance in the marine ecosystem. [Display omitted]
•Very few studies have examined the antimicrobial resistance in enterococci in fecal samples of wild marine species.•This study report the species distribution and occurrence of resistant-enterococci in feces of wild marine species.•Antibiotic resistance in enterococci isolated from these animals is an important gap that should be addressed.•Antibiotic resistant strains could be related to food web interactions, aquatic pollutants or environmental resistome.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>26952995</pmid><doi>10.1016/j.marpolbul.2016.02.071</doi><tpages>7</tpages></addata></record> |
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| ispartof | Marine pollution bulletin, 2016-04, Vol.105 (1), p.51-57 |
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| subjects | Animals Anti-Bacterial Agents - pharmacology antibiotic resistance Aquatic Organisms - microbiology biogeography Brazil coasts Drug Resistance, Bacterial - genetics Drug Resistance, Microbial Enterococci resistant Enterococcus - isolation & purification Enterococcus faecalis Enterococcus faecalis - genetics Enterococcus faecium Enterococcus faecium - genetics Environmental Monitoring feces Feces - microbiology food webs genes mammals Marine Microbial Sensitivity Tests phenotype pollutants Polymerase Chain Reaction Resistance genes rifampicin sea turtles seabirds water pollution Wild marine species |
| title | Resistance to antimicrobial agents among enterococci isolated from fecal samples of wild marine species in the southern coast of Brazil |
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