Cell‐bound exopolysaccharides of Lactobacillus brevis KB290 enhance cytotoxic activity of mouse splenocytes
AIMS: This study aimed to identify the main active component of Lactobacillus brevis KB290 (KB290) that is responsible for enhanced cell‐mediated cytotoxic activity of mouse splenocytes Live KB290, a probiotic strain derived from a Japanese traditional pickle, was previously reported to modulate inn...
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| Veröffentlicht in: | Journal of applied microbiology 2015-02, Vol.118 (2), p.506-514 |
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| creator | Sasaki, E Suzuki, S Fukui, Y Yajima, N |
| description | AIMS: This study aimed to identify the main active component of Lactobacillus brevis KB290 (KB290) that is responsible for enhanced cell‐mediated cytotoxic activity of mouse splenocytes Live KB290, a probiotic strain derived from a Japanese traditional pickle, was previously reported to modulate innate immune responses as affecting on cell‐mediated cytotoxic activity of mouse splenocytes. METHODS AND RESULTS: We used live KB290, heat‐killed KB290, a derivative strain (Lact. brevis KB392) with different amounts of cell‐bound exopolysaccharide (EPS‐b), and a crude extract of EPS‐b from KB290 cell surface. Female BALB/c mice were fed a diet containing 10¹⁰ CFU live KB290, 10¹⁰ CFU live KB392, 15 mg heat‐killed KB290 or 600 μg crude extract of EPS‐b for 1 day. Live KB290 (P |
| doi_str_mv | 10.1111/jam.12686 |
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METHODS AND RESULTS: We used live KB290, heat‐killed KB290, a derivative strain (Lact. brevis KB392) with different amounts of cell‐bound exopolysaccharide (EPS‐b), and a crude extract of EPS‐b from KB290 cell surface. Female BALB/c mice were fed a diet containing 10¹⁰ CFU live KB290, 10¹⁰ CFU live KB392, 15 mg heat‐killed KB290 or 600 μg crude extract of EPS‐b for 1 day. Live KB290 (P < 0·01), heat‐killed KB290 (P < 0·05) and crude EPS‐b at 600 μg (P < 0·05) per mouse significantly enhanced cytotoxic activity; however, live KB392 had no effect. CONCLUSIONS: Both live and heat‐killed KB290 and crude EPS‐b significantly enhanced cytotoxic activity of mouse splenocytes. SIGNIFICANCE AND IMPACT OF THE STUDY: We demonstrated that EPS‐b produced by KB290 has a critical role in enhancing cell‐mediated cytotoxic activity in mouse spleen.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.12686</identifier><identifier>PMID: 25376258</identifier><language>eng</language><publisher>England: Published for the Society for Applied Bacteriology by Blackwell Science</publisher><subject>Animals ; cell‐bound exopolysaccharides ; cell‐mediated cytotoxicity ; cytotoxicity ; Cytotoxicity, Immunologic - drug effects ; diet ; exopolysaccharides ; Female ; females ; heat‐killed KB290 ; innate immunity ; Lactobacillus brevis ; Lactobacillus brevis KB290 ; Lactobacillus brevis KB392 ; mice ; Mice, Inbred BALB C ; pickles ; Polysaccharides, Bacterial - chemistry ; Polysaccharides, Bacterial - pharmacology ; probiotics ; Probiotics - pharmacology ; spleen ; Spleen - immunology ; splenocytes</subject><ispartof>Journal of applied microbiology, 2015-02, Vol.118 (2), p.506-514</ispartof><rights>2014 The Society for Applied Microbiology</rights><rights>2014 The Society for Applied Microbiology.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5536-3284333f4ddccdcc2cb719dbe0dae00fdfde5e95a33493d84947486ee26850dc3</citedby><cites>FETCH-LOGICAL-c5536-3284333f4ddccdcc2cb719dbe0dae00fdfde5e95a33493d84947486ee26850dc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjam.12686$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjam.12686$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25376258$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sasaki, E</creatorcontrib><creatorcontrib>Suzuki, S</creatorcontrib><creatorcontrib>Fukui, Y</creatorcontrib><creatorcontrib>Yajima, N</creatorcontrib><title>Cell‐bound exopolysaccharides of Lactobacillus brevis KB290 enhance cytotoxic activity of mouse splenocytes</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>AIMS: This study aimed to identify the main active component of Lactobacillus brevis KB290 (KB290) that is responsible for enhanced cell‐mediated cytotoxic activity of mouse splenocytes Live KB290, a probiotic strain derived from a Japanese traditional pickle, was previously reported to modulate innate immune responses as affecting on cell‐mediated cytotoxic activity of mouse splenocytes. METHODS AND RESULTS: We used live KB290, heat‐killed KB290, a derivative strain (Lact. brevis KB392) with different amounts of cell‐bound exopolysaccharide (EPS‐b), and a crude extract of EPS‐b from KB290 cell surface. Female BALB/c mice were fed a diet containing 10¹⁰ CFU live KB290, 10¹⁰ CFU live KB392, 15 mg heat‐killed KB290 or 600 μg crude extract of EPS‐b for 1 day. Live KB290 (P < 0·01), heat‐killed KB290 (P < 0·05) and crude EPS‐b at 600 μg (P < 0·05) per mouse significantly enhanced cytotoxic activity; however, live KB392 had no effect. CONCLUSIONS: Both live and heat‐killed KB290 and crude EPS‐b significantly enhanced cytotoxic activity of mouse splenocytes. SIGNIFICANCE AND IMPACT OF THE STUDY: We demonstrated that EPS‐b produced by KB290 has a critical role in enhancing cell‐mediated cytotoxic activity in mouse spleen.</description><subject>Animals</subject><subject>cell‐bound exopolysaccharides</subject><subject>cell‐mediated cytotoxicity</subject><subject>cytotoxicity</subject><subject>Cytotoxicity, Immunologic - drug effects</subject><subject>diet</subject><subject>exopolysaccharides</subject><subject>Female</subject><subject>females</subject><subject>heat‐killed KB290</subject><subject>innate immunity</subject><subject>Lactobacillus brevis</subject><subject>Lactobacillus brevis KB290</subject><subject>Lactobacillus brevis KB392</subject><subject>mice</subject><subject>Mice, Inbred BALB C</subject><subject>pickles</subject><subject>Polysaccharides, Bacterial - chemistry</subject><subject>Polysaccharides, Bacterial - pharmacology</subject><subject>probiotics</subject><subject>Probiotics - pharmacology</subject><subject>spleen</subject><subject>Spleen - immunology</subject><subject>splenocytes</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctO3TAQhi3UCihlwQsUL9tFwJf4tqRHvR_URcvacuxJMUriQ5xQsusj9Bn7JDUc6A51NNLM4ptfM_MjdETJCS1xeuX6E8qkljton3IpKiYVe3bf15Ugiu2hFzlfEUI5EXIX7THBlWRC76N-BV3359fvJs1DwHCbNqlbsvP-0o0xQMapxWvnp9Q4H7tuzrgZ4SZm_OUtMwTDcOkGD9gvU5rSbfS4sPEmTsvdYJ_mDDhvOhhSISC_RM9b12U4fKgH6OL9u--rj9X664dPq7N15YXgsuJM15zztg7B-5LMN4qa0AAJDghpQxtAgBGO89rwoGtTq1pLgPIDQYLnB-j1VnczpusZ8mT7mH251A1QdrJUaWWUIdr8H5WC1YRLxQv6Zov6MeU8Qms3Y-zduFhK7J0Rthhh740o7KsH2bnpIfwjHz9fgNMt8DN2sDytZD-fnT9KHm8nWpes-zHGbC--MUJF8VVraRT_C7Iynb4</recordid><startdate>201502</startdate><enddate>201502</enddate><creator>Sasaki, E</creator><creator>Suzuki, S</creator><creator>Fukui, Y</creator><creator>Yajima, N</creator><general>Published for the Society for Applied Bacteriology by Blackwell Science</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7ST</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>SOI</scope></search><sort><creationdate>201502</creationdate><title>Cell‐bound exopolysaccharides of Lactobacillus brevis KB290 enhance cytotoxic activity of mouse splenocytes</title><author>Sasaki, E ; Suzuki, S ; Fukui, Y ; Yajima, N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5536-3284333f4ddccdcc2cb719dbe0dae00fdfde5e95a33493d84947486ee26850dc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>cell‐bound exopolysaccharides</topic><topic>cell‐mediated cytotoxicity</topic><topic>cytotoxicity</topic><topic>Cytotoxicity, Immunologic - drug effects</topic><topic>diet</topic><topic>exopolysaccharides</topic><topic>Female</topic><topic>females</topic><topic>heat‐killed KB290</topic><topic>innate immunity</topic><topic>Lactobacillus brevis</topic><topic>Lactobacillus brevis KB290</topic><topic>Lactobacillus brevis KB392</topic><topic>mice</topic><topic>Mice, Inbred BALB C</topic><topic>pickles</topic><topic>Polysaccharides, Bacterial - chemistry</topic><topic>Polysaccharides, Bacterial - pharmacology</topic><topic>probiotics</topic><topic>Probiotics - pharmacology</topic><topic>spleen</topic><topic>Spleen - immunology</topic><topic>splenocytes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sasaki, E</creatorcontrib><creatorcontrib>Suzuki, S</creatorcontrib><creatorcontrib>Fukui, Y</creatorcontrib><creatorcontrib>Yajima, N</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sasaki, E</au><au>Suzuki, S</au><au>Fukui, Y</au><au>Yajima, N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cell‐bound exopolysaccharides of Lactobacillus brevis KB290 enhance cytotoxic activity of mouse splenocytes</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2015-02</date><risdate>2015</risdate><volume>118</volume><issue>2</issue><spage>506</spage><epage>514</epage><pages>506-514</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>AIMS: This study aimed to identify the main active component of Lactobacillus brevis KB290 (KB290) that is responsible for enhanced cell‐mediated cytotoxic activity of mouse splenocytes Live KB290, a probiotic strain derived from a Japanese traditional pickle, was previously reported to modulate innate immune responses as affecting on cell‐mediated cytotoxic activity of mouse splenocytes. METHODS AND RESULTS: We used live KB290, heat‐killed KB290, a derivative strain (Lact. brevis KB392) with different amounts of cell‐bound exopolysaccharide (EPS‐b), and a crude extract of EPS‐b from KB290 cell surface. Female BALB/c mice were fed a diet containing 10¹⁰ CFU live KB290, 10¹⁰ CFU live KB392, 15 mg heat‐killed KB290 or 600 μg crude extract of EPS‐b for 1 day. Live KB290 (P < 0·01), heat‐killed KB290 (P < 0·05) and crude EPS‐b at 600 μg (P < 0·05) per mouse significantly enhanced cytotoxic activity; however, live KB392 had no effect. CONCLUSIONS: Both live and heat‐killed KB290 and crude EPS‐b significantly enhanced cytotoxic activity of mouse splenocytes. SIGNIFICANCE AND IMPACT OF THE STUDY: We demonstrated that EPS‐b produced by KB290 has a critical role in enhancing cell‐mediated cytotoxic activity in mouse spleen.</abstract><cop>England</cop><pub>Published for the Society for Applied Bacteriology by Blackwell Science</pub><pmid>25376258</pmid><doi>10.1111/jam.12686</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); Wiley Online Library All Journals |
| subjects | Animals cell‐bound exopolysaccharides cell‐mediated cytotoxicity cytotoxicity Cytotoxicity, Immunologic - drug effects diet exopolysaccharides Female females heat‐killed KB290 innate immunity Lactobacillus brevis Lactobacillus brevis KB290 Lactobacillus brevis KB392 mice Mice, Inbred BALB C pickles Polysaccharides, Bacterial - chemistry Polysaccharides, Bacterial - pharmacology probiotics Probiotics - pharmacology spleen Spleen - immunology splenocytes |
| title | Cell‐bound exopolysaccharides of Lactobacillus brevis KB290 enhance cytotoxic activity of mouse splenocytes |
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