Extensive developmental and metabolic alterations in cybrids Nicotiana tabacum (+ Hyoscyamus niger) are caused by complex nucleo‐cytoplasmic incompatibility
Summary The genetic basis of multiple phenotypic alterations was studied in cell‐engineered cybrids Nicotiana tabacum (+ Hyoscyamus niger) combining the nuclear genome of N. tabacum, plastome of H. niger and recombinant mitochondria. The plants possess a complex, maternally inheritable syndrome of n...
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creator | Zubko, Mikhajlo K. Zubko, Elena I. Ruban, Alexander V. Adler, Klaus Mock, Hans‐Peter Misera, Simon Gleba, Yuri YU Grimm, Bernhard |
description | Summary
The genetic basis of multiple phenotypic alterations was studied in cell‐engineered cybrids Nicotiana tabacum (+ Hyoscyamus niger) combining the nuclear genome of N. tabacum, plastome of H. niger and recombinant mitochondria. The plants possess a complex, maternally inheritable syndrome of nucleo‐cytoplasmic incompatibility, severely affecting growth, metabolism and development. In vivo, the syndrome was manifested as: late germination of seeds; dramatic decrease of chlorophyll and carotenoids in cotyledons and leaves; altered morphology of cotyledons, leaves and flowers; and dwarfism. The leaf phenotype depended on light intensity. In ‘green flowers’ (an extreme phenotype), homeotic function B was downregulated. In vitro, the incompatibility syndrome was restricted to the pigment deficiency of cotyledons. Electron microscopy revealed perturbations in the differentiation of chloroplasts and palisade parenchyma cells in bleached leaves. The pigment deficiency accompanied by retarded growth is discussed as a result of plastome–genome incompatibility, whereas other features are likely to be due to nucleo‐mitochondrial incompatibilities. |
doi_str_mv | 10.1046/j.1365-313x.2001.00997.x |
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The genetic basis of multiple phenotypic alterations was studied in cell‐engineered cybrids Nicotiana tabacum (+ Hyoscyamus niger) combining the nuclear genome of N. tabacum, plastome of H. niger and recombinant mitochondria. The plants possess a complex, maternally inheritable syndrome of nucleo‐cytoplasmic incompatibility, severely affecting growth, metabolism and development. In vivo, the syndrome was manifested as: late germination of seeds; dramatic decrease of chlorophyll and carotenoids in cotyledons and leaves; altered morphology of cotyledons, leaves and flowers; and dwarfism. The leaf phenotype depended on light intensity. In ‘green flowers’ (an extreme phenotype), homeotic function B was downregulated. In vitro, the incompatibility syndrome was restricted to the pigment deficiency of cotyledons. Electron microscopy revealed perturbations in the differentiation of chloroplasts and palisade parenchyma cells in bleached leaves. The pigment deficiency accompanied by retarded growth is discussed as a result of plastome–genome incompatibility, whereas other features are likely to be due to nucleo‐mitochondrial incompatibilities.</description><identifier>ISSN: 0960-7412</identifier><identifier>EISSN: 1365-313X</identifier><identifier>DOI: 10.1046/j.1365-313x.2001.00997.x</identifier><identifier>PMID: 11319030</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Agronomy. Soil science and plant productions ; Biological and medical sciences ; carotenoids ; Cell Fusion ; Cell Nucleus - genetics ; Cell Nucleus - physiology ; Chimera - genetics ; Chimera - growth & development ; Chimera - metabolism ; chloroplast biogenesis ; Chloroplasts - genetics ; Chloroplasts - ultrastructure ; Cotyledon - ultrastructure ; cybrids ; Cytoplasm - genetics ; Cytoplasm - physiology ; cytoplasmic homeosis model ; DNA, Mitochondrial ; DNA, Recombinant ; Fundamental and applied biological sciences. Psychology ; Genes, Homeobox ; Genes, Plant ; Genetic Engineering ; Genetics and breeding of economic plants ; Hyoscyamus ; Hyoscyamus niger ; Interspecific and intergeneric hybridization, introgressions ; maternal inheritance ; Nicotiana ; Nicotiana - genetics ; Nicotiana - physiology ; Nicotiana tabacum ; nucleo-cytoplasmic incompatibility ; Phenotype ; Pigments, Biological - analysis ; Plant breeding: fundamental aspects and methodology ; Plant Stems - genetics ; Plant Stems - growth & development ; Plants, Toxic ; plastomes ; Solanaceae - genetics ; Solanaceae - physiology</subject><ispartof>The Plant journal : for cell and molecular biology, 2001-03, Vol.25 (6), p.627-639</ispartof><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5397-2dc0b63cc5f81fa952ca774b16f74e7c9c3c4d93e2eaede01867e0b88d16b9d23</citedby><cites>FETCH-LOGICAL-c5397-2dc0b63cc5f81fa952ca774b16f74e7c9c3c4d93e2eaede01867e0b88d16b9d23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-313x.2001.00997.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-313x.2001.00997.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=978176$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11319030$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zubko, Mikhajlo K.</creatorcontrib><creatorcontrib>Zubko, Elena I.</creatorcontrib><creatorcontrib>Ruban, Alexander V.</creatorcontrib><creatorcontrib>Adler, Klaus</creatorcontrib><creatorcontrib>Mock, Hans‐Peter</creatorcontrib><creatorcontrib>Misera, Simon</creatorcontrib><creatorcontrib>Gleba, Yuri YU</creatorcontrib><creatorcontrib>Grimm, Bernhard</creatorcontrib><title>Extensive developmental and metabolic alterations in cybrids Nicotiana tabacum (+ Hyoscyamus niger) are caused by complex nucleo‐cytoplasmic incompatibility</title><title>The Plant journal : for cell and molecular biology</title><addtitle>Plant J</addtitle><description>Summary
The genetic basis of multiple phenotypic alterations was studied in cell‐engineered cybrids Nicotiana tabacum (+ Hyoscyamus niger) combining the nuclear genome of N. tabacum, plastome of H. niger and recombinant mitochondria. The plants possess a complex, maternally inheritable syndrome of nucleo‐cytoplasmic incompatibility, severely affecting growth, metabolism and development. In vivo, the syndrome was manifested as: late germination of seeds; dramatic decrease of chlorophyll and carotenoids in cotyledons and leaves; altered morphology of cotyledons, leaves and flowers; and dwarfism. The leaf phenotype depended on light intensity. In ‘green flowers’ (an extreme phenotype), homeotic function B was downregulated. In vitro, the incompatibility syndrome was restricted to the pigment deficiency of cotyledons. Electron microscopy revealed perturbations in the differentiation of chloroplasts and palisade parenchyma cells in bleached leaves. The pigment deficiency accompanied by retarded growth is discussed as a result of plastome–genome incompatibility, whereas other features are likely to be due to nucleo‐mitochondrial incompatibilities.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Biological and medical sciences</subject><subject>carotenoids</subject><subject>Cell Fusion</subject><subject>Cell Nucleus - genetics</subject><subject>Cell Nucleus - physiology</subject><subject>Chimera - genetics</subject><subject>Chimera - growth & development</subject><subject>Chimera - metabolism</subject><subject>chloroplast biogenesis</subject><subject>Chloroplasts - genetics</subject><subject>Chloroplasts - ultrastructure</subject><subject>Cotyledon - ultrastructure</subject><subject>cybrids</subject><subject>Cytoplasm - genetics</subject><subject>Cytoplasm - physiology</subject><subject>cytoplasmic homeosis model</subject><subject>DNA, Mitochondrial</subject><subject>DNA, Recombinant</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Homeobox</subject><subject>Genes, Plant</subject><subject>Genetic Engineering</subject><subject>Genetics and breeding of economic plants</subject><subject>Hyoscyamus</subject><subject>Hyoscyamus niger</subject><subject>Interspecific and intergeneric hybridization, introgressions</subject><subject>maternal inheritance</subject><subject>Nicotiana</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana - physiology</subject><subject>Nicotiana tabacum</subject><subject>nucleo-cytoplasmic incompatibility</subject><subject>Phenotype</subject><subject>Pigments, Biological - analysis</subject><subject>Plant breeding: fundamental aspects and methodology</subject><subject>Plant Stems - genetics</subject><subject>Plant Stems - growth & development</subject><subject>Plants, Toxic</subject><subject>plastomes</subject><subject>Solanaceae - genetics</subject><subject>Solanaceae - physiology</subject><issn>0960-7412</issn><issn>1365-313X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcGK1EAQhoMo7uzqK0iDIIpM7E5n0mnYiyyrqyzqYQVvTaVSkR463TGdrMltYV_AR_DZ9knMOKNePVVBfX_9hy9JmOCp4HnxapsKWWzWUsgpzTgXKedaq3S6l6z-HL7cT1ZcF3ytcpEdJccxbhdQySJ_mBwJIYXmkq-Sn-fTQD7aa2I1XZMLXUt-AMfA16ylAargLDJwA_Uw2OAjs57hXPW2juyDxTBY8MAWEHBs2fOXdze3F3OIOEM7RubtV-pfMOiJIYyRalbNDEPbOZqYH9FRuLv5gfMQOgexXaqs352Xrso6O8yPkgcNuEiPD_Mk-fzm_OrsYn358e27s9eXa9xIrdZZjbwqJOKmKUUDepMhKJVXomhUTgo1SsxrLSkjoJq4KAtFvCrLWhSVrjN5kjzb_-368G2kOJjWRiTnwFMYoxGqVCLL9QKWexD7EGNPjel620I_G8HNzo7Zmp0Es7NjdnbMbztmWqJPDh1j1VL9L3jQsQBPDwBEBNf04NHGv5xWpVDFQp3uqe_W0fzf9ebq0_tlkb8AemKyBA</recordid><startdate>200103</startdate><enddate>200103</enddate><creator>Zubko, Mikhajlo K.</creator><creator>Zubko, Elena I.</creator><creator>Ruban, Alexander V.</creator><creator>Adler, Klaus</creator><creator>Mock, Hans‐Peter</creator><creator>Misera, Simon</creator><creator>Gleba, Yuri YU</creator><creator>Grimm, Bernhard</creator><general>Blackwell Science Ltd</general><general>Blackwell Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>200103</creationdate><title>Extensive developmental and metabolic alterations in cybrids Nicotiana tabacum (+ Hyoscyamus niger) are caused by complex nucleo‐cytoplasmic incompatibility</title><author>Zubko, Mikhajlo K. ; Zubko, Elena I. ; Ruban, Alexander V. ; Adler, Klaus ; Mock, Hans‐Peter ; Misera, Simon ; Gleba, Yuri YU ; Grimm, Bernhard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5397-2dc0b63cc5f81fa952ca774b16f74e7c9c3c4d93e2eaede01867e0b88d16b9d23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Biological and medical sciences</topic><topic>carotenoids</topic><topic>Cell Fusion</topic><topic>Cell Nucleus - genetics</topic><topic>Cell Nucleus - physiology</topic><topic>Chimera - genetics</topic><topic>Chimera - growth & development</topic><topic>Chimera - metabolism</topic><topic>chloroplast biogenesis</topic><topic>Chloroplasts - genetics</topic><topic>Chloroplasts - ultrastructure</topic><topic>Cotyledon - ultrastructure</topic><topic>cybrids</topic><topic>Cytoplasm - genetics</topic><topic>Cytoplasm - physiology</topic><topic>cytoplasmic homeosis model</topic><topic>DNA, Mitochondrial</topic><topic>DNA, Recombinant</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Homeobox</topic><topic>Genes, Plant</topic><topic>Genetic Engineering</topic><topic>Genetics and breeding of economic plants</topic><topic>Hyoscyamus</topic><topic>Hyoscyamus niger</topic><topic>Interspecific and intergeneric hybridization, introgressions</topic><topic>maternal inheritance</topic><topic>Nicotiana</topic><topic>Nicotiana - genetics</topic><topic>Nicotiana - physiology</topic><topic>Nicotiana tabacum</topic><topic>nucleo-cytoplasmic incompatibility</topic><topic>Phenotype</topic><topic>Pigments, Biological - analysis</topic><topic>Plant breeding: fundamental aspects and methodology</topic><topic>Plant Stems - genetics</topic><topic>Plant Stems - growth & development</topic><topic>Plants, Toxic</topic><topic>plastomes</topic><topic>Solanaceae - genetics</topic><topic>Solanaceae - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zubko, Mikhajlo K.</creatorcontrib><creatorcontrib>Zubko, Elena I.</creatorcontrib><creatorcontrib>Ruban, Alexander V.</creatorcontrib><creatorcontrib>Adler, Klaus</creatorcontrib><creatorcontrib>Mock, Hans‐Peter</creatorcontrib><creatorcontrib>Misera, Simon</creatorcontrib><creatorcontrib>Gleba, Yuri YU</creatorcontrib><creatorcontrib>Grimm, Bernhard</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>The Plant journal : for cell and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zubko, Mikhajlo K.</au><au>Zubko, Elena I.</au><au>Ruban, Alexander V.</au><au>Adler, Klaus</au><au>Mock, Hans‐Peter</au><au>Misera, Simon</au><au>Gleba, Yuri YU</au><au>Grimm, Bernhard</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Extensive developmental and metabolic alterations in cybrids Nicotiana tabacum (+ Hyoscyamus niger) are caused by complex nucleo‐cytoplasmic incompatibility</atitle><jtitle>The Plant journal : for cell and molecular biology</jtitle><addtitle>Plant J</addtitle><date>2001-03</date><risdate>2001</risdate><volume>25</volume><issue>6</issue><spage>627</spage><epage>639</epage><pages>627-639</pages><issn>0960-7412</issn><eissn>1365-313X</eissn><abstract>Summary
The genetic basis of multiple phenotypic alterations was studied in cell‐engineered cybrids Nicotiana tabacum (+ Hyoscyamus niger) combining the nuclear genome of N. tabacum, plastome of H. niger and recombinant mitochondria. The plants possess a complex, maternally inheritable syndrome of nucleo‐cytoplasmic incompatibility, severely affecting growth, metabolism and development. In vivo, the syndrome was manifested as: late germination of seeds; dramatic decrease of chlorophyll and carotenoids in cotyledons and leaves; altered morphology of cotyledons, leaves and flowers; and dwarfism. The leaf phenotype depended on light intensity. In ‘green flowers’ (an extreme phenotype), homeotic function B was downregulated. In vitro, the incompatibility syndrome was restricted to the pigment deficiency of cotyledons. Electron microscopy revealed perturbations in the differentiation of chloroplasts and palisade parenchyma cells in bleached leaves. The pigment deficiency accompanied by retarded growth is discussed as a result of plastome–genome incompatibility, whereas other features are likely to be due to nucleo‐mitochondrial incompatibilities.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>11319030</pmid><doi>10.1046/j.1365-313x.2001.00997.x</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Agronomy. Soil science and plant productions Biological and medical sciences carotenoids Cell Fusion Cell Nucleus - genetics Cell Nucleus - physiology Chimera - genetics Chimera - growth & development Chimera - metabolism chloroplast biogenesis Chloroplasts - genetics Chloroplasts - ultrastructure Cotyledon - ultrastructure cybrids Cytoplasm - genetics Cytoplasm - physiology cytoplasmic homeosis model DNA, Mitochondrial DNA, Recombinant Fundamental and applied biological sciences. Psychology Genes, Homeobox Genes, Plant Genetic Engineering Genetics and breeding of economic plants Hyoscyamus Hyoscyamus niger Interspecific and intergeneric hybridization, introgressions maternal inheritance Nicotiana Nicotiana - genetics Nicotiana - physiology Nicotiana tabacum nucleo-cytoplasmic incompatibility Phenotype Pigments, Biological - analysis Plant breeding: fundamental aspects and methodology Plant Stems - genetics Plant Stems - growth & development Plants, Toxic plastomes Solanaceae - genetics Solanaceae - physiology |
title | Extensive developmental and metabolic alterations in cybrids Nicotiana tabacum (+ Hyoscyamus niger) are caused by complex nucleo‐cytoplasmic incompatibility |
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