Dead-time correction of fluorescence lifetime measurements and fluorescence lifetime imaging

Dead-time correction of fluorescence lifetime measurements and fluorescence lifetime imaging

We present a comprehensive theory of dead-time effects on Time-Correlated Single Photon Counting (TCSPC) as used for fluorescence lifetime measurements, and develop a correction algorithm to remove these artifacts. We apply this algorithm to fluorescence lifetime measurements as well as to Fluoresce...

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Veröffentlicht in:Optics express 2016-05, Vol.24 (9), p.9429-9445
Hauptverfasser: Isbaner, Sebastian, Karedla, Narain, Ruhlandt, Daja, Stein, Simon Christoph, Chizhik, Anna, Gregor, Ingo, Enderlein, Jörg
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container_end_page 9445
container_issue 9
container_start_page 9429
container_title Optics express
container_volume 24
creator Isbaner, Sebastian
Karedla, Narain
Ruhlandt, Daja
Stein, Simon Christoph
Chizhik, Anna
Gregor, Ingo
Enderlein, Jörg
description We present a comprehensive theory of dead-time effects on Time-Correlated Single Photon Counting (TCSPC) as used for fluorescence lifetime measurements, and develop a correction algorithm to remove these artifacts. We apply this algorithm to fluorescence lifetime measurements as well as to Fluorescence Lifetime Imaging Microscopy (FLIM), where rapid data acquisition is necessarily connected with high count rates. There, dead-time effects cannot be neglected, and lead to distortions in the observed lifetime image. The algorithm is quite general and completely independent of the particular nature of the measured signal. It can also be applied to any other single-event counting measurement with detector and/or electronics dead-time.
doi_str_mv 10.1364/OE.24.009429
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title Dead-time correction of fluorescence lifetime measurements and fluorescence lifetime imaging
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