Modern affinity reagents: Recombinant antibodies and aptamers
Affinity reagents are essential tools in both basic and applied research; however, there is a growing concern about the reproducibility of animal-derived monoclonal antibodies. The need for higher quality affinity reagents has prompted the development of methods that provide scientific, economic, an...
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| Veröffentlicht in: | Biotechnology advances 2015-12, Vol.33 (8), p.1787-1798 |
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| container_title | Biotechnology advances |
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| creator | Groff, Katherine Brown, Jeffrey Clippinger, Amy J. |
| description | Affinity reagents are essential tools in both basic and applied research; however, there is a growing concern about the reproducibility of animal-derived monoclonal antibodies. The need for higher quality affinity reagents has prompted the development of methods that provide scientific, economic, and time-saving advantages and do not require the use of animals. This review describes two types of affinity reagents, recombinant antibodies and aptamers, which are non-animal technologies that can replace the use of animal-derived monoclonal antibodies. Recombinant antibodies are protein-based reagents, while aptamers are nucleic-acid-based. In light of the scientific advantages of these technologies, this review also discusses ways to gain momentum in the use of modern affinity reagents, including an update to the 1999 National Academy of Sciences monoclonal antibody production report and federal incentives for recombinant antibody and aptamer efforts. In the long-term, these efforts have the potential to improve the overall quality and decrease the cost of scientific research. |
| doi_str_mv | 10.1016/j.biotechadv.2015.10.004 |
| format | Article |
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In the long-term, these efforts have the potential to improve the overall quality and decrease the cost of scientific research.</description><subject>Affinity</subject><subject>Affinity reagents</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies - immunology</subject><subject>Antibodies - metabolism</subject><subject>Aptamers</subject><subject>Aptamers, Nucleotide - biosynthesis</subject><subject>Aptamers, Nucleotide - genetics</subject><subject>Ascites antibodies</subject><subject>Biotechnology</subject><subject>Gain</subject><subject>Monoclonal antibodies</subject><subject>Reagents</subject><subject>Recombinant</subject><subject>Recombinant antibodies</subject><subject>Recombinant Proteins - biosynthesis</subject><subject>Recombinant Proteins - immunology</subject><subject>Reproducibility</subject><subject>SELEX Aptamer Technique</subject><issn>0734-9750</issn><issn>1873-1899</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1P3DAQhi3UCraUv4By5JLt-CN2jNRDi4BWoqqE6NlyxhPwapNs7SwS_x6vFsqxHKyxPM_MK_lhrOKw5MD1l9Wyi9NM-ODD41IAb8rzEkAdsAVvjax5a-0HtgAjVW1NA0fsU84rKCA08pAdCa1aAVIt2NdfU6A0Vr7v4xjnpyqRv6dxzufVLeE0dHH041yVE7spRMrlGiq_mf1AKX9mH3u_znTyUo_Zn6vLu4sf9c3v658X325qVBbm2uvQCO2FAa9b6Iz2RqK0KNuWS-QQUNu-F6IT1lJA5EidajigMT32Hcljdrbfu0nT3y3l2Q0xI63XfqRpmx03rebWcg7vQRshFQj7DlRZzZVVqqDtHsU05Zyod5sUB5-eHAe3U-JW7k2J2ynZdYqSMnr6krLtBgr_Bl8dFOD7HqDyg4-RkssYaUQKMRHOLkzx_ynPOGyhLw</recordid><startdate>20151201</startdate><enddate>20151201</enddate><creator>Groff, Katherine</creator><creator>Brown, Jeffrey</creator><creator>Clippinger, Amy J.</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7U5</scope><scope>L7M</scope></search><sort><creationdate>20151201</creationdate><title>Modern affinity reagents: Recombinant antibodies and aptamers</title><author>Groff, Katherine ; 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| issn | 0734-9750 1873-1899 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Affinity Affinity reagents Animals Antibodies Antibodies - immunology Antibodies - metabolism Aptamers Aptamers, Nucleotide - biosynthesis Aptamers, Nucleotide - genetics Ascites antibodies Biotechnology Gain Monoclonal antibodies Reagents Recombinant Recombinant antibodies Recombinant Proteins - biosynthesis Recombinant Proteins - immunology Reproducibility SELEX Aptamer Technique |
| title | Modern affinity reagents: Recombinant antibodies and aptamers |
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