3D Droplet Microfluidic Systems for High-Throughput Biological Experimentation
Herein, we describe the development of a multilayer droplet microfluidic system for creating concentration gradients and generating microdroplets of varying composition for high-throughput biochemical and cell-based screening applications. The 3D droplet-based microfluidic device consists of multipl...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2015-11, Vol.87 (21), p.10770-10778 |
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| container_title | Analytical chemistry (Washington) |
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| creator | Kang, Dong-Ku Gong, Xiuqing Cho, Soongwon Kim, Jin-young Edel, Joshua B Chang, Soo-Ik Choo, Jaebum deMello, Andrew J |
| description | Herein, we describe the development of a multilayer droplet microfluidic system for creating concentration gradients and generating microdroplets of varying composition for high-throughput biochemical and cell-based screening applications. The 3D droplet-based microfluidic device consists of multiple PDMS layers, which are used to generate logarithmic concentration gradient reagent profiles. Parallel flow focusing structures are used to form picoliter-sized droplets of defined volumes but of varying composition. As proof of concept, we demonstrate rapid enzymatic activity assays and drug cytotoxicity assays on bacteria. The 3D droplet-based microfluidic platform has the potential to allow for high-efficiency and high-throughput analysis, overcoming the structural limitations of single layer microfluidic systems. |
| doi_str_mv | 10.1021/acs.analchem.5b02402 |
| format | Article |
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The 3D droplet-based microfluidic platform has the potential to allow for high-efficiency and high-throughput analysis, overcoming the structural limitations of single layer microfluidic systems.</description><subject>Analytical chemistry</subject><subject>Anti-Bacterial Agents - chemistry</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Assaying</subject><subject>Bacteria</subject><subject>Bacteria - drug effects</subject><subject>Bacteria - enzymology</subject><subject>Bioassays</subject><subject>Biochemistry</subject><subject>Caspase 3 - chemistry</subject><subject>Caspase 3 - metabolism</subject><subject>Cell Survival - drug effects</subject><subject>Chemistry Techniques, Analytical - methods</subject><subject>Concentration gradient</subject><subject>Cytotoxicity</subject><subject>Devices</subject><subject>Droplets</subject><subject>Enzymes</subject><subject>Kinetics</subject><subject>Microfluidic Analytical Techniques</subject><subject>Microfluidics</subject><subject>Nanotechnology</subject><subject>Three dimensional</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUtLxDAUhYMoOj7-gUjBjZuON7dpmyx1HB_gY-G4LmmazkTaSU1a0H9vdEYFF-IqcPnOCZyPkEMKYwpIT6XyY7mUjVrodpyWgAxwg4xoihBnnOMmGQFAEmMOsEN2vX8GoBRotk12MGNMcAEjcp9cRBfOdo3uozujnK2bwVRGRY9vvtetj2rromszX8SzhbPDfNENfXRubGPnRskmmr522plWL3vZG7vcJ1u1bLw-WL975OlyOptcx7cPVzeTs9tYMpb1MVUgUWjMK55gKXiikhJrxnVVUkw50-EGFHOGvJYMNRWcK0qFhErVyGSyR05WvZ2zL4P2fdEar3TTyKW2gy9ozjMqEkzFP9AEwp6CYUCPf6HPdnBh408KOcvTPA0UW1FhLe-drosuLCDdW0Gh-FBTBDXFl5pirSbEjtblQ9nq6jv05SIAsAI-4j8f_9X5DmSYm_E</recordid><startdate>20151103</startdate><enddate>20151103</enddate><creator>Kang, Dong-Ku</creator><creator>Gong, Xiuqing</creator><creator>Cho, Soongwon</creator><creator>Kim, Jin-young</creator><creator>Edel, Joshua B</creator><creator>Chang, Soo-Ik</creator><creator>Choo, Jaebum</creator><creator>deMello, Andrew J</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20151103</creationdate><title>3D Droplet Microfluidic Systems for High-Throughput Biological Experimentation</title><author>Kang, Dong-Ku ; Gong, Xiuqing ; Cho, Soongwon ; Kim, Jin-young ; Edel, Joshua B ; Chang, Soo-Ik ; Choo, Jaebum ; deMello, Andrew J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a446t-1c0a29e27d832b983c3b2f48edb12584eb980127428fa42e1988c119a0dcf24a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Analytical chemistry</topic><topic>Anti-Bacterial Agents - chemistry</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Assaying</topic><topic>Bacteria</topic><topic>Bacteria - drug effects</topic><topic>Bacteria - enzymology</topic><topic>Bioassays</topic><topic>Biochemistry</topic><topic>Caspase 3 - chemistry</topic><topic>Caspase 3 - metabolism</topic><topic>Cell Survival - drug effects</topic><topic>Chemistry Techniques, Analytical - methods</topic><topic>Concentration gradient</topic><topic>Cytotoxicity</topic><topic>Devices</topic><topic>Droplets</topic><topic>Enzymes</topic><topic>Kinetics</topic><topic>Microfluidic Analytical Techniques</topic><topic>Microfluidics</topic><topic>Nanotechnology</topic><topic>Three dimensional</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kang, Dong-Ku</creatorcontrib><creatorcontrib>Gong, Xiuqing</creatorcontrib><creatorcontrib>Cho, Soongwon</creatorcontrib><creatorcontrib>Kim, Jin-young</creatorcontrib><creatorcontrib>Edel, Joshua B</creatorcontrib><creatorcontrib>Chang, Soo-Ik</creatorcontrib><creatorcontrib>Choo, Jaebum</creatorcontrib><creatorcontrib>deMello, Andrew J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kang, Dong-Ku</au><au>Gong, Xiuqing</au><au>Cho, Soongwon</au><au>Kim, Jin-young</au><au>Edel, Joshua B</au><au>Chang, Soo-Ik</au><au>Choo, Jaebum</au><au>deMello, Andrew J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>3D Droplet Microfluidic Systems for High-Throughput Biological Experimentation</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. 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| source | ACS Publications; MEDLINE |
| subjects | Analytical chemistry Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - pharmacology Assaying Bacteria Bacteria - drug effects Bacteria - enzymology Bioassays Biochemistry Caspase 3 - chemistry Caspase 3 - metabolism Cell Survival - drug effects Chemistry Techniques, Analytical - methods Concentration gradient Cytotoxicity Devices Droplets Enzymes Kinetics Microfluidic Analytical Techniques Microfluidics Nanotechnology Three dimensional |
| title | 3D Droplet Microfluidic Systems for High-Throughput Biological Experimentation |
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