Rapid, cost-effective DNA quantification via a visually-detectable aggregation of superparamagnetic silica-magnetite nanoparticles
DNA and silica-coated magnetic particles entangle and form visible aggregates under chaotropic conditions with a rotating magnetic field, in a manner that enables quantification of DNA by image analysis. As a means of exploring the mechanism of this DNA quantitation assay, nanoscale SiO 2 -coated Fe...
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| Veröffentlicht in: | Nano research 2014-05, Vol.7 (5), p.755-764 |
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| creator | Liu, Qian Li, Jingyi Liu, Hongxue Tora, Ibrahim Ide, Matthew S. Lu, Jiwei Davis, Robert J. Green, David L. Landers, James P. |
| description | DNA and silica-coated magnetic particles entangle and form visible aggregates under chaotropic conditions with a rotating magnetic field, in a manner that enables quantification of DNA by image analysis. As a means of exploring the mechanism of this DNA quantitation assay, nanoscale SiO
2
-coated Fe
3
O
4
(Fe
3
O
4
@SiO
2
) particles are synthesized via a solvothermal method. Characterization of the particles defines them to be ∼200 nm in diameter with a large surface area (141.89 m
2
/g), possessing superparamagnetic properties and exhibiting high saturation magnetization (38 emu/g). The synthesized Fe
3
O
4
@SiO
2
nanoparticles are exploited in the DNA quantification assay and, as predicted, the nanoparticles provide better sensitivity than commercial microscale Dynabeads® for quantifying DNA, with a detection limit of 4 kilobase-pair fragments of human DNA. Their utility is proven using nanoparticle DNA quantification to guide efficient polymerase chain reaction (PCR) amplification of short tandem repeat loci for human identification. |
| doi_str_mv | 10.1007/s12274-014-0436-9 |
| format | Article |
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2
-coated Fe
3
O
4
(Fe
3
O
4
@SiO
2
) particles are synthesized via a solvothermal method. Characterization of the particles defines them to be ∼200 nm in diameter with a large surface area (141.89 m
2
/g), possessing superparamagnetic properties and exhibiting high saturation magnetization (38 emu/g). The synthesized Fe
3
O
4
@SiO
2
nanoparticles are exploited in the DNA quantification assay and, as predicted, the nanoparticles provide better sensitivity than commercial microscale Dynabeads® for quantifying DNA, with a detection limit of 4 kilobase-pair fragments of human DNA. Their utility is proven using nanoparticle DNA quantification to guide efficient polymerase chain reaction (PCR) amplification of short tandem repeat loci for human identification.</description><identifier>ISSN: 1998-0124</identifier><identifier>EISSN: 1998-0000</identifier><identifier>DOI: 10.1007/s12274-014-0436-9</identifier><language>eng</language><publisher>Heidelberg: Tsinghua University Press</publisher><subject>Assaying ; Atomic/Molecular Structure and Spectra ; Biomedicine ; Biotechnology ; Chemistry and Materials Science ; Condensed Matter Physics ; Deoxyribonucleic acid ; DNA ; Fragments ; Human ; Loci ; Magnetic fields ; Materials Science ; Microscopy ; Nanoparticles ; Nanostructure ; Nanotechnology ; Polymerase chain reaction ; Research Article ; Silica ; Silicon dioxide</subject><ispartof>Nano research, 2014-05, Vol.7 (5), p.755-764</ispartof><rights>Tsinghua University Press and Springer-Verlag Berlin Heidelberg 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c382t-56ebcde45cf697a8abc2f4987447edc5f9b10e4ff81482c871f1aa6239a6cd633</citedby><cites>FETCH-LOGICAL-c382t-56ebcde45cf697a8abc2f4987447edc5f9b10e4ff81482c871f1aa6239a6cd633</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12274-014-0436-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12274-014-0436-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Liu, Qian</creatorcontrib><creatorcontrib>Li, Jingyi</creatorcontrib><creatorcontrib>Liu, Hongxue</creatorcontrib><creatorcontrib>Tora, Ibrahim</creatorcontrib><creatorcontrib>Ide, Matthew S.</creatorcontrib><creatorcontrib>Lu, Jiwei</creatorcontrib><creatorcontrib>Davis, Robert J.</creatorcontrib><creatorcontrib>Green, David L.</creatorcontrib><creatorcontrib>Landers, James P.</creatorcontrib><title>Rapid, cost-effective DNA quantification via a visually-detectable aggregation of superparamagnetic silica-magnetite nanoparticles</title><title>Nano research</title><addtitle>Nano Res</addtitle><description>DNA and silica-coated magnetic particles entangle and form visible aggregates under chaotropic conditions with a rotating magnetic field, in a manner that enables quantification of DNA by image analysis. As a means of exploring the mechanism of this DNA quantitation assay, nanoscale SiO
2
-coated Fe
3
O
4
(Fe
3
O
4
@SiO
2
) particles are synthesized via a solvothermal method. Characterization of the particles defines them to be ∼200 nm in diameter with a large surface area (141.89 m
2
/g), possessing superparamagnetic properties and exhibiting high saturation magnetization (38 emu/g). The synthesized Fe
3
O
4
@SiO
2
nanoparticles are exploited in the DNA quantification assay and, as predicted, the nanoparticles provide better sensitivity than commercial microscale Dynabeads® for quantifying DNA, with a detection limit of 4 kilobase-pair fragments of human DNA. Their utility is proven using nanoparticle DNA quantification to guide efficient polymerase chain reaction (PCR) amplification of short tandem repeat loci for human identification.</description><subject>Assaying</subject><subject>Atomic/Molecular Structure and Spectra</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>Chemistry and Materials Science</subject><subject>Condensed Matter Physics</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Fragments</subject><subject>Human</subject><subject>Loci</subject><subject>Magnetic fields</subject><subject>Materials Science</subject><subject>Microscopy</subject><subject>Nanoparticles</subject><subject>Nanostructure</subject><subject>Nanotechnology</subject><subject>Polymerase chain reaction</subject><subject>Research Article</subject><subject>Silica</subject><subject>Silicon 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quantification via a visually-detectable aggregation of superparamagnetic silica-magnetite nanoparticles</atitle><jtitle>Nano research</jtitle><stitle>Nano Res</stitle><date>2014-05-01</date><risdate>2014</risdate><volume>7</volume><issue>5</issue><spage>755</spage><epage>764</epage><pages>755-764</pages><issn>1998-0124</issn><eissn>1998-0000</eissn><abstract>DNA and silica-coated magnetic particles entangle and form visible aggregates under chaotropic conditions with a rotating magnetic field, in a manner that enables quantification of DNA by image analysis. As a means of exploring the mechanism of this DNA quantitation assay, nanoscale SiO
2
-coated Fe
3
O
4
(Fe
3
O
4
@SiO
2
) particles are synthesized via a solvothermal method. Characterization of the particles defines them to be ∼200 nm in diameter with a large surface area (141.89 m
2
/g), possessing superparamagnetic properties and exhibiting high saturation magnetization (38 emu/g). The synthesized Fe
3
O
4
@SiO
2
nanoparticles are exploited in the DNA quantification assay and, as predicted, the nanoparticles provide better sensitivity than commercial microscale Dynabeads® for quantifying DNA, with a detection limit of 4 kilobase-pair fragments of human DNA. Their utility is proven using nanoparticle DNA quantification to guide efficient polymerase chain reaction (PCR) amplification of short tandem repeat loci for human identification.</abstract><cop>Heidelberg</cop><pub>Tsinghua University Press</pub><doi>10.1007/s12274-014-0436-9</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1998-0124 |
| ispartof | Nano research, 2014-05, Vol.7 (5), p.755-764 |
| issn | 1998-0124 1998-0000 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1786177447 |
| source | SpringerLink Journals |
| subjects | Assaying Atomic/Molecular Structure and Spectra Biomedicine Biotechnology Chemistry and Materials Science Condensed Matter Physics Deoxyribonucleic acid DNA Fragments Human Loci Magnetic fields Materials Science Microscopy Nanoparticles Nanostructure Nanotechnology Polymerase chain reaction Research Article Silica Silicon dioxide |
| title | Rapid, cost-effective DNA quantification via a visually-detectable aggregation of superparamagnetic silica-magnetite nanoparticles |
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