Azurin-DNA Conjugate with the Binding Motif of a Transcriptional Regulator, CooA
An azurin-DNA conjugate (Azu-DNA) where the DNA part contains the binding sequence of a carbon monoxide (CO)-dependent transcriptional regulator, CooA, has been prepared. CooA in the CO-bound form (CO-CooA) showed specific binding to Azu-DNA. The association of CO-CooA with Azu-DNA affects the elect...
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| Veröffentlicht in: | Chemistry letters 2014-01, Vol.43 (8), p.1204-1204 |
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| container_title | Chemistry letters |
| container_volume | 43 |
| creator | Nakajima, Hiroshi Miyazaki, Soji Itoh, Takaaki Hayamura, Mao Watanabe, Yoshihito |
| description | An azurin-DNA conjugate (Azu-DNA) where the DNA part contains the binding sequence of a carbon monoxide (CO)-dependent transcriptional regulator, CooA, has been prepared. CooA in the CO-bound form (CO-CooA) showed specific binding to Azu-DNA. The association of CO-CooA with Azu-DNA affects the electron transfer (ET) between Azu-DNA and its redox partner protein, cytochrome c552. Consequently, the CO-dependent modulation of ET was achieved. This could be a potential platform for a novel biobased sensor, which exploits the transcriptional regulator as a detection module and whose readout is immediately transduced to a readily processable electronic signal. |
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CooA in the CO-bound form (CO-CooA) showed specific binding to Azu-DNA. The association of CO-CooA with Azu-DNA affects the electron transfer (ET) between Azu-DNA and its redox partner protein, cytochrome c552. Consequently, the CO-dependent modulation of ET was achieved. This could be a potential platform for a novel biobased sensor, which exploits the transcriptional regulator as a detection module and whose readout is immediately transduced to a readily processable electronic signal.</description><identifier>ISSN: 0366-7022</identifier><identifier>EISSN: 1348-0715</identifier><language>eng</language><publisher>Tokyo: Chemical Society of Japan</publisher><subject>Binding ; Carbon monoxide ; Conjugates ; Deoxyribonucleic acid ; Electronics ; Modulation ; Platforms ; Regulators</subject><ispartof>Chemistry letters, 2014-01, Vol.43 (8), p.1204-1204</ispartof><rights>Copyright Japan Science and Technology Agency 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids></links><search><creatorcontrib>Nakajima, Hiroshi</creatorcontrib><creatorcontrib>Miyazaki, Soji</creatorcontrib><creatorcontrib>Itoh, Takaaki</creatorcontrib><creatorcontrib>Hayamura, Mao</creatorcontrib><creatorcontrib>Watanabe, Yoshihito</creatorcontrib><title>Azurin-DNA Conjugate with the Binding Motif of a Transcriptional Regulator, CooA</title><title>Chemistry letters</title><description>An azurin-DNA conjugate (Azu-DNA) where the DNA part contains the binding sequence of a carbon monoxide (CO)-dependent transcriptional regulator, CooA, has been prepared. CooA in the CO-bound form (CO-CooA) showed specific binding to Azu-DNA. The association of CO-CooA with Azu-DNA affects the electron transfer (ET) between Azu-DNA and its redox partner protein, cytochrome c552. Consequently, the CO-dependent modulation of ET was achieved. This could be a potential platform for a novel biobased sensor, which exploits the transcriptional regulator as a detection module and whose readout is immediately transduced to a readily processable electronic signal.</description><subject>Binding</subject><subject>Carbon monoxide</subject><subject>Conjugates</subject><subject>Deoxyribonucleic acid</subject><subject>Electronics</subject><subject>Modulation</subject><subject>Platforms</subject><subject>Regulators</subject><issn>0366-7022</issn><issn>1348-0715</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNqNz8tKxDAYBeAgCtbRdwi4cWEhyd8k7bLWK4wXpPshbZNOSk1qkyL49FZ05crV2XycwzlACYUsT4mk_BAlBIRIJWHsGJ2EMBBC8gJYgl7Kz2W2Lr1-KnHl3bD0Kmr8YeMex73GV9Z11vX40UdrsDdY4XpWLrSznaL1To34VffLqKKfL9cCX56iI6PGoM9-c4Pq25u6uk-3z3cPVblNpyLjace6vAOgpGDQNLlkrSmAS6qh5bJpKKdac9qRjspGGZIZUMZAA0JKYSijsEEXP7XT7N8XHeLuzYZWj6Ny2i9hR2UuqGCF4P-ggoHM1_WVnv-hg1_m9eW34lAQwmQGX__GZgA</recordid><startdate>20140101</startdate><enddate>20140101</enddate><creator>Nakajima, Hiroshi</creator><creator>Miyazaki, Soji</creator><creator>Itoh, Takaaki</creator><creator>Hayamura, Mao</creator><creator>Watanabe, Yoshihito</creator><general>Chemical Society of Japan</general><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>7TM</scope></search><sort><creationdate>20140101</creationdate><title>Azurin-DNA Conjugate with the Binding Motif of a Transcriptional Regulator, CooA</title><author>Nakajima, Hiroshi ; Miyazaki, Soji ; Itoh, Takaaki ; Hayamura, Mao ; Watanabe, Yoshihito</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p945-d2d8d3310923bb872cf93571e3c57bb151ee51d0d17baf04f3aff3b36776f1213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Binding</topic><topic>Carbon monoxide</topic><topic>Conjugates</topic><topic>Deoxyribonucleic acid</topic><topic>Electronics</topic><topic>Modulation</topic><topic>Platforms</topic><topic>Regulators</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nakajima, Hiroshi</creatorcontrib><creatorcontrib>Miyazaki, Soji</creatorcontrib><creatorcontrib>Itoh, Takaaki</creatorcontrib><creatorcontrib>Hayamura, Mao</creatorcontrib><creatorcontrib>Watanabe, Yoshihito</creatorcontrib><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Chemistry letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nakajima, Hiroshi</au><au>Miyazaki, Soji</au><au>Itoh, Takaaki</au><au>Hayamura, Mao</au><au>Watanabe, Yoshihito</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Azurin-DNA Conjugate with the Binding Motif of a Transcriptional Regulator, CooA</atitle><jtitle>Chemistry letters</jtitle><date>2014-01-01</date><risdate>2014</risdate><volume>43</volume><issue>8</issue><spage>1204</spage><epage>1204</epage><pages>1204-1204</pages><issn>0366-7022</issn><eissn>1348-0715</eissn><abstract>An azurin-DNA conjugate (Azu-DNA) where the DNA part contains the binding sequence of a carbon monoxide (CO)-dependent transcriptional regulator, CooA, has been prepared. CooA in the CO-bound form (CO-CooA) showed specific binding to Azu-DNA. The association of CO-CooA with Azu-DNA affects the electron transfer (ET) between Azu-DNA and its redox partner protein, cytochrome c552. Consequently, the CO-dependent modulation of ET was achieved. This could be a potential platform for a novel biobased sensor, which exploits the transcriptional regulator as a detection module and whose readout is immediately transduced to a readily processable electronic signal.</abstract><cop>Tokyo</cop><pub>Chemical Society of Japan</pub><tpages>1</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0366-7022 |
| ispartof | Chemistry letters, 2014-01, Vol.43 (8), p.1204-1204 |
| issn | 0366-7022 1348-0715 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1786162965 |
| source | Oxford University Press Journals All Titles (1996-Current) |
| subjects | Binding Carbon monoxide Conjugates Deoxyribonucleic acid Electronics Modulation Platforms Regulators |
| title | Azurin-DNA Conjugate with the Binding Motif of a Transcriptional Regulator, CooA |
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