Synthesis and Properties of Novel Fluorescently Labeled Oligonucleotides Possessing Silylated Pyrene at the 5′-Terminus
Previously, we reported a novel fluorescence dye, a silylated pyrene derivative, as a DNA labeling agent. In this study, new silylated pyrene derivatives with different linker lengths and different substituents on the silicon atom have been prepared and introduced into oligonucleotides. In particula...
Gespeichert in:
| Veröffentlicht in: | Chemistry letters 2015, Vol.44 (1), p.44-46 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 46 |
|---|---|
| container_issue | 1 |
| container_start_page | 44 |
| container_title | Chemistry letters |
| container_volume | 44 |
| creator | Moriguchi, Tomohisa Moki, Daisuke Sekiguchi, Tohru Kato, Takahiko Shinozuka, Kazuo |
| description | Previously, we reported a novel fluorescence dye, a silylated pyrene derivative, as a DNA labeling agent. In this study, new silylated pyrene derivatives with different linker lengths and different substituents on the silicon atom have been prepared and introduced into oligonucleotides. In particular, when the diphenylsilylpyrene derivative was introduced as a fluorescence dye, the fluorescence intensity of the duplex was enhanced about 20 times compared to the signal in the absence of the target DNA. |
| doi_str_mv | 10.1246/cl.140800 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1786162219</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1786162219</sourcerecordid><originalsourceid>FETCH-LOGICAL-c329t-14df10930e1c3db02f58c264d50303fd70800c90f2e557cc85d7c637cf94478b3</originalsourceid><addsrcrecordid>eNqNkbFOHDEQhq2ISDlIiryBJZpQLBnba-9uiRAEpFM4CVKvfN5Z8MlnH7YXabs8Ux4pTxKfjgIlTappvvlG_z-EfGZwznitvhp3zmpoAd6RBRN1W0HD5BFZgFCqaoDzD-Q4pQ0AtJ3gCzLfzz4_YbKJaj_QVQw7jNliomGk38MLOnrtphAxGfTZzXSp1-hwoHfOPgY_GYch26Hwq5ASpmT9I723bnY6F2o1R_RIdablCJW_f_6qHjBurZ_SR_J-1C7hp9d5Qn5cXz1c3lTLu2-3lxfLygje5YrVw8igE4DMiGENfJSt4aoeJAgQ49Dsw5oORo5SNsa0cmiMEo0Zu7pu2rU4IV8O3l0MzxOm3G9tCeOc9him1LOmVUxxzrr_QBUXsi09F_T0L3QTpuhLkEJJ0ZWmxV54dqBMLO1EHPtdtFsd555Bv_9Xb1x_-Fdh1Sv7hFtriikYi3ne6J32b-z_LP4BqXabsQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1753902239</pqid></control><display><type>article</type><title>Synthesis and Properties of Novel Fluorescently Labeled Oligonucleotides Possessing Silylated Pyrene at the 5′-Terminus</title><source>Oxford Journals</source><creator>Moriguchi, Tomohisa ; Moki, Daisuke ; Sekiguchi, Tohru ; Kato, Takahiko ; Shinozuka, Kazuo</creator><creatorcontrib>Moriguchi, Tomohisa ; Moki, Daisuke ; Sekiguchi, Tohru ; Kato, Takahiko ; Shinozuka, Kazuo</creatorcontrib><description>Previously, we reported a novel fluorescence dye, a silylated pyrene derivative, as a DNA labeling agent. In this study, new silylated pyrene derivatives with different linker lengths and different substituents on the silicon atom have been prepared and introduced into oligonucleotides. In particular, when the diphenylsilylpyrene derivative was introduced as a fluorescence dye, the fluorescence intensity of the duplex was enhanced about 20 times compared to the signal in the absence of the target DNA.</description><identifier>ISSN: 0366-7022</identifier><identifier>EISSN: 1348-0715</identifier><identifier>DOI: 10.1246/cl.140800</identifier><language>eng</language><publisher>Tokyo: The Chemical Society of Japan</publisher><subject>Deoxyribonucleic acid ; Derivatives ; Dyes ; Fluorescence ; Oligonucleotides ; Pyrenes ; Silicon ; Synthesis</subject><ispartof>Chemistry letters, 2015, Vol.44 (1), p.44-46</ispartof><rights>The Chemical Society of Japan</rights><rights>Copyright Japan Science and Technology Agency 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c329t-14df10930e1c3db02f58c264d50303fd70800c90f2e557cc85d7c637cf94478b3</citedby><cites>FETCH-LOGICAL-c329t-14df10930e1c3db02f58c264d50303fd70800c90f2e557cc85d7c637cf94478b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids></links><search><creatorcontrib>Moriguchi, Tomohisa</creatorcontrib><creatorcontrib>Moki, Daisuke</creatorcontrib><creatorcontrib>Sekiguchi, Tohru</creatorcontrib><creatorcontrib>Kato, Takahiko</creatorcontrib><creatorcontrib>Shinozuka, Kazuo</creatorcontrib><title>Synthesis and Properties of Novel Fluorescently Labeled Oligonucleotides Possessing Silylated Pyrene at the 5′-Terminus</title><title>Chemistry letters</title><addtitle>Chemistry Letters</addtitle><description>Previously, we reported a novel fluorescence dye, a silylated pyrene derivative, as a DNA labeling agent. In this study, new silylated pyrene derivatives with different linker lengths and different substituents on the silicon atom have been prepared and introduced into oligonucleotides. In particular, when the diphenylsilylpyrene derivative was introduced as a fluorescence dye, the fluorescence intensity of the duplex was enhanced about 20 times compared to the signal in the absence of the target DNA.</description><subject>Deoxyribonucleic acid</subject><subject>Derivatives</subject><subject>Dyes</subject><subject>Fluorescence</subject><subject>Oligonucleotides</subject><subject>Pyrenes</subject><subject>Silicon</subject><subject>Synthesis</subject><issn>0366-7022</issn><issn>1348-0715</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNqNkbFOHDEQhq2ISDlIiryBJZpQLBnba-9uiRAEpFM4CVKvfN5Z8MlnH7YXabs8Ux4pTxKfjgIlTappvvlG_z-EfGZwznitvhp3zmpoAd6RBRN1W0HD5BFZgFCqaoDzD-Q4pQ0AtJ3gCzLfzz4_YbKJaj_QVQw7jNliomGk38MLOnrtphAxGfTZzXSp1-hwoHfOPgY_GYch26Hwq5ASpmT9I723bnY6F2o1R_RIdablCJW_f_6qHjBurZ_SR_J-1C7hp9d5Qn5cXz1c3lTLu2-3lxfLygje5YrVw8igE4DMiGENfJSt4aoeJAgQ49Dsw5oORo5SNsa0cmiMEo0Zu7pu2rU4IV8O3l0MzxOm3G9tCeOc9him1LOmVUxxzrr_QBUXsi09F_T0L3QTpuhLkEJJ0ZWmxV54dqBMLO1EHPtdtFsd555Bv_9Xb1x_-Fdh1Sv7hFtriikYi3ne6J32b-z_LP4BqXabsQ</recordid><startdate>2015</startdate><enddate>2015</enddate><creator>Moriguchi, Tomohisa</creator><creator>Moki, Daisuke</creator><creator>Sekiguchi, Tohru</creator><creator>Kato, Takahiko</creator><creator>Shinozuka, Kazuo</creator><general>The Chemical Society of Japan</general><general>Chemical Society of Japan</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>7TM</scope></search><sort><creationdate>2015</creationdate><title>Synthesis and Properties of Novel Fluorescently Labeled Oligonucleotides Possessing Silylated Pyrene at the 5′-Terminus</title><author>Moriguchi, Tomohisa ; Moki, Daisuke ; Sekiguchi, Tohru ; Kato, Takahiko ; Shinozuka, Kazuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c329t-14df10930e1c3db02f58c264d50303fd70800c90f2e557cc85d7c637cf94478b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Deoxyribonucleic acid</topic><topic>Derivatives</topic><topic>Dyes</topic><topic>Fluorescence</topic><topic>Oligonucleotides</topic><topic>Pyrenes</topic><topic>Silicon</topic><topic>Synthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moriguchi, Tomohisa</creatorcontrib><creatorcontrib>Moki, Daisuke</creatorcontrib><creatorcontrib>Sekiguchi, Tohru</creatorcontrib><creatorcontrib>Kato, Takahiko</creatorcontrib><creatorcontrib>Shinozuka, Kazuo</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Chemistry letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moriguchi, Tomohisa</au><au>Moki, Daisuke</au><au>Sekiguchi, Tohru</au><au>Kato, Takahiko</au><au>Shinozuka, Kazuo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synthesis and Properties of Novel Fluorescently Labeled Oligonucleotides Possessing Silylated Pyrene at the 5′-Terminus</atitle><jtitle>Chemistry letters</jtitle><addtitle>Chemistry Letters</addtitle><date>2015</date><risdate>2015</risdate><volume>44</volume><issue>1</issue><spage>44</spage><epage>46</epage><pages>44-46</pages><issn>0366-7022</issn><eissn>1348-0715</eissn><abstract>Previously, we reported a novel fluorescence dye, a silylated pyrene derivative, as a DNA labeling agent. In this study, new silylated pyrene derivatives with different linker lengths and different substituents on the silicon atom have been prepared and introduced into oligonucleotides. In particular, when the diphenylsilylpyrene derivative was introduced as a fluorescence dye, the fluorescence intensity of the duplex was enhanced about 20 times compared to the signal in the absence of the target DNA.</abstract><cop>Tokyo</cop><pub>The Chemical Society of Japan</pub><doi>10.1246/cl.140800</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0366-7022 |
| ispartof | Chemistry letters, 2015, Vol.44 (1), p.44-46 |
| issn | 0366-7022 1348-0715 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1786162219 |
| source | Oxford Journals |
| subjects | Deoxyribonucleic acid Derivatives Dyes Fluorescence Oligonucleotides Pyrenes Silicon Synthesis |
| title | Synthesis and Properties of Novel Fluorescently Labeled Oligonucleotides Possessing Silylated Pyrene at the 5′-Terminus |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T10%3A44%3A21IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Synthesis%20and%20Properties%20of%20Novel%20Fluorescently%20Labeled%20Oligonucleotides%20Possessing%20Silylated%20Pyrene%20at%20the%205%E2%80%B2-Terminus&rft.jtitle=Chemistry%20letters&rft.au=Moriguchi,%20Tomohisa&rft.date=2015&rft.volume=44&rft.issue=1&rft.spage=44&rft.epage=46&rft.pages=44-46&rft.issn=0366-7022&rft.eissn=1348-0715&rft_id=info:doi/10.1246/cl.140800&rft_dat=%3Cproquest_cross%3E1786162219%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1753902239&rft_id=info:pmid/&rfr_iscdi=true |