A Microbead-based Single Base Extension Assay for the Detection of Known Single-base Changes in Genomic DNA

Biotinylated single base extension (SBE) products that can fluoresce through the successful incorporation of fluorophore-labeled dideoxynucleoside triphosphate were immobilized on streptavidin-coated magnetic bead surfaces via the biotin–streptavidin interaction. The resultant beads were washed and...

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Veröffentlicht in:	Chemistry letters 2015, Vol.44 (5), p.595-597
Hauptverfasser:	Fujishima, Kohei, Yamamoto, Shunsuke, Tsukagoshi, Kazuhiko, Hashimoto, Masahiko
Format:	Artikel
Sprache:	eng
Schlagworte:	Assaying Beads Capillarity Deoxyribonucleic acid Fluorescence Gene sequencing Resultants Strategy
Online-Zugang:	Volltext
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