A new approach for the construction of dual character in nanosystems

•We synthesized dual character nanoenzyme systems by using a new approach.•This multienzyme-nanoparticle synthesis approach can effectively be used in enzyme engineering and biotechnology.•Thermal stability of the prepared nanoparticles is considerably enhanced by the photosensitive crosslinking polymerization.•The prepared dual character nanoparticles are very stable even after 14 cycle usage. This study describes the preparation of nanoprotein particles carrying horseradish peroxidase (HRP) and alkaline phosphatase (ALP) using a photosensitive microemulsion polymerization technique. The prepared nanoparticles having dual character were characterized by transmission electron microscopy (TEM) and Zeta Sizer. The average particle size of p(HRP-co-ALP) nanoparticles was found to be about 90nm. The activity of nanoparticles having dual character was assayed using tetramethylbenzidine and paranitrophenyl phosphate (p-NPP) as substrates for HRP and ALP, respectively. The parameters such as pH, temperature and initial enzyme concentration that affect the activity were investigated by using p(HRP-co-ALP) nanoparticles and compared with the free HRP and ALP. The reusability of p(HRP-co-ALP) particles was also investigated and the results showed that the prepared nanoparticles exhibited admirable potential as a reusable catalyst.