Simultaneous Determination of Tramadol and O-Desmethyltramadol in Human Plasma Using HPLC–DAD

A sensitive and accurate method for the extraction and quantification of tramadol (T) and its active metabolite, O-desmethyltramadol (ODT) in human plasma with high-performance liquid chromatography–diode array detection was developed and validated. The analytes were extracted from plasma samples by...

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Veröffentlicht in:	Journal of chromatographic science 2014-11, Vol.52 (10), p.1186-1192
Hauptverfasser:	Hilal, Maha A., Mohamed, Khaled M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetonitriles - chemistry Ammonium hydroxide Buffers Calibration Chromatography Chromatography, High Pressure Liquid - methods Drug Stability Ethers Extraction Hospitals Human Humans Limit of Detection Linear Models Liquids Phosphates - chemistry Potassium Compounds - chemistry Propranolol - blood Reproducibility of Results Tramadol - analogs & derivatives Tramadol - blood
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