An Alternative Method for the Genetic Transformation of Sweet Orange
An alternative method for transforming sweet orange [Citrus sinensis (L.) Osbeck] has been developed. Plasmid DNA encoding the non-destructive selectable marker enhanced green fluorescent protein gene was introduced using polyethylene glycol into protoplasts of 'Itaborai' sweet orange isol...
Gespeichert in:
| Veröffentlicht in: | In vitro cellular & developmental biology. Plant 2000-11, Vol.36 (6), p.450-455 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 455 |
|---|---|
| container_issue | 6 |
| container_start_page | 450 |
| container_title | In vitro cellular & developmental biology. Plant |
| container_volume | 36 |
| creator | Fleming, G. H. O. Olivares-Fuster S. Fatta Del-Bosco J. W. Grosser |
| description | An alternative method for transforming sweet orange [Citrus sinensis (L.) Osbeck] has been developed. Plasmid DNA encoding the non-destructive selectable marker enhanced green fluorescent protein gene was introduced using polyethylene glycol into protoplasts of 'Itaborai' sweet orange isolated from an embryogenic nucellar-derived suspension culture. Following protoplast culture in liquid medium and transfer to solid medium, transformed calluses were identified via expression of the green fluorescent protein, physically separated from non-transformed tissue, and cultured on somatic embryogenesis induction medium. Transgenic plantlets were recovered from germinating somatic embryos and by in vitro rooting of shoots. To expedite transgenic plant recovery, regenerated shoots were also micrografted onto sour orange seedling rootstocks. Presence of the transgene in calluses and regenerated sweet orange plants was verified by gene amplification and Southern analyses. Potential advantages of this transformation system over the commonly used Agrobacterium methods for citrus are discussed. |
| doi_str_mv | 10.1007/s11627-000-0081-8 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_17860782</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>4293388</jstor_id><sourcerecordid>4293388</sourcerecordid><originalsourceid>FETCH-LOGICAL-j260t-8d486c3a758585962d01e6b99314bd0882708100dcf118dde6db4317d42d03833</originalsourceid><addsrcrecordid>eNpdz81KAzEQAOAgCtbqAwgegoK31fxtMjmWqlWo9GA9L-kma3fZbmqSKr69kYoHCUOGmY9hBqFzSm4oIeo2UiqZKgghOYAWcIBGVKiyYBL0Yc5JKYpSKHmMTmLssqOEqhG6mwx40icXBpPaD4efXVp7ixsfcFo7PHODS22Nl8EMMRc3WfkB-wa_fDqX8CLX39wpOmpMH93Z7z9Grw_3y-ljMV_MnqaTedExSVIBVoCsuVEl5Kcls4Q6udKaU7GyBICpvDohtm4oBWudtCvBqbIiSw6cj9H1fu42-Pedi6natLF2fW8G53exogokUcAyvPwHO7_LJ_bZaMV1KZnM6OoXmVibvsmn1G2stqHdmPBVgVZAfkZd7FUXkw9_XcE05wD8G5Pubhw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>197395626</pqid></control><display><type>article</type><title>An Alternative Method for the Genetic Transformation of Sweet Orange</title><source>Jstor Complete Legacy</source><source>SpringerLink Journals</source><creator>Fleming, G. H. ; O. Olivares-Fuster ; S. Fatta Del-Bosco ; J. W. Grosser</creator><creatorcontrib>Fleming, G. H. ; O. Olivares-Fuster ; S. Fatta Del-Bosco ; J. W. Grosser</creatorcontrib><description>An alternative method for transforming sweet orange [Citrus sinensis (L.) Osbeck] has been developed. Plasmid DNA encoding the non-destructive selectable marker enhanced green fluorescent protein gene was introduced using polyethylene glycol into protoplasts of 'Itaborai' sweet orange isolated from an embryogenic nucellar-derived suspension culture. Following protoplast culture in liquid medium and transfer to solid medium, transformed calluses were identified via expression of the green fluorescent protein, physically separated from non-transformed tissue, and cultured on somatic embryogenesis induction medium. Transgenic plantlets were recovered from germinating somatic embryos and by in vitro rooting of shoots. To expedite transgenic plant recovery, regenerated shoots were also micrografted onto sour orange seedling rootstocks. Presence of the transgene in calluses and regenerated sweet orange plants was verified by gene amplification and Southern analyses. Potential advantages of this transformation system over the commonly used Agrobacterium methods for citrus are discussed.</description><identifier>ISSN: 1054-5476</identifier><identifier>EISSN: 1475-2689</identifier><identifier>DOI: 10.1007/s11627-000-0081-8</identifier><language>eng</language><publisher>Wallingford: CABI Publishing</publisher><subject>Agrobacterium ; Agronomy. Soil science and plant productions ; Biological and medical sciences ; Biotechnology ; Biotechnology/Genetic Transformation/Functional Genomics ; Callus ; Citrus sinensis ; DNA ; Fundamental and applied biological sciences. Psychology ; Genetic engineering ; Genetic engineering applications ; Genetic technics ; Genetic transformation ; Genetics and breeding of economic plants ; green fluorescent protein ; Methods. Procedures. Technologies ; Plant breeding: fundamental aspects and methodology ; Plants ; Plasmids ; polyethylene glycol ; Protoplast culture ; Protoplasts ; Seedlings ; Somatic embryos ; Transgenic animals and transgenic plants ; Transgenic plants</subject><ispartof>In vitro cellular & developmental biology. Plant, 2000-11, Vol.36 (6), p.450-455</ispartof><rights>Copyright 2000 The Society for In Vitro Biology</rights><rights>2001 INIST-CNRS</rights><rights>Copyright Society for In Vitro Biology Nov/Dec 2000</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4293388$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4293388$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>309,310,314,776,780,785,786,799,23909,23910,25118,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=897802$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Fleming, G. H.</creatorcontrib><creatorcontrib>O. Olivares-Fuster</creatorcontrib><creatorcontrib>S. Fatta Del-Bosco</creatorcontrib><creatorcontrib>J. W. Grosser</creatorcontrib><title>An Alternative Method for the Genetic Transformation of Sweet Orange</title><title>In vitro cellular & developmental biology. Plant</title><description>An alternative method for transforming sweet orange [Citrus sinensis (L.) Osbeck] has been developed. Plasmid DNA encoding the non-destructive selectable marker enhanced green fluorescent protein gene was introduced using polyethylene glycol into protoplasts of 'Itaborai' sweet orange isolated from an embryogenic nucellar-derived suspension culture. Following protoplast culture in liquid medium and transfer to solid medium, transformed calluses were identified via expression of the green fluorescent protein, physically separated from non-transformed tissue, and cultured on somatic embryogenesis induction medium. Transgenic plantlets were recovered from germinating somatic embryos and by in vitro rooting of shoots. To expedite transgenic plant recovery, regenerated shoots were also micrografted onto sour orange seedling rootstocks. Presence of the transgene in calluses and regenerated sweet orange plants was verified by gene amplification and Southern analyses. Potential advantages of this transformation system over the commonly used Agrobacterium methods for citrus are discussed.</description><subject>Agrobacterium</subject><subject>Agronomy. Soil science and plant productions</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Biotechnology/Genetic Transformation/Functional Genomics</subject><subject>Callus</subject><subject>Citrus sinensis</subject><subject>DNA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic engineering</subject><subject>Genetic engineering applications</subject><subject>Genetic technics</subject><subject>Genetic transformation</subject><subject>Genetics and breeding of economic plants</subject><subject>green fluorescent protein</subject><subject>Methods. Procedures. Technologies</subject><subject>Plant breeding: fundamental aspects and methodology</subject><subject>Plants</subject><subject>Plasmids</subject><subject>polyethylene glycol</subject><subject>Protoplast culture</subject><subject>Protoplasts</subject><subject>Seedlings</subject><subject>Somatic embryos</subject><subject>Transgenic animals and transgenic plants</subject><subject>Transgenic plants</subject><issn>1054-5476</issn><issn>1475-2689</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNpdz81KAzEQAOAgCtbqAwgegoK31fxtMjmWqlWo9GA9L-kma3fZbmqSKr69kYoHCUOGmY9hBqFzSm4oIeo2UiqZKgghOYAWcIBGVKiyYBL0Yc5JKYpSKHmMTmLssqOEqhG6mwx40icXBpPaD4efXVp7ixsfcFo7PHODS22Nl8EMMRc3WfkB-wa_fDqX8CLX39wpOmpMH93Z7z9Grw_3y-ljMV_MnqaTedExSVIBVoCsuVEl5Kcls4Q6udKaU7GyBICpvDohtm4oBWudtCvBqbIiSw6cj9H1fu42-Pedi6natLF2fW8G53exogokUcAyvPwHO7_LJ_bZaMV1KZnM6OoXmVibvsmn1G2stqHdmPBVgVZAfkZd7FUXkw9_XcE05wD8G5Pubhw</recordid><startdate>20001101</startdate><enddate>20001101</enddate><creator>Fleming, G. H.</creator><creator>O. Olivares-Fuster</creator><creator>S. Fatta Del-Bosco</creator><creator>J. W. Grosser</creator><general>CABI Publishing</general><general>Cambridge University Press</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>3V.</scope><scope>4T-</scope><scope>4U-</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>S0X</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20001101</creationdate><title>An Alternative Method for the Genetic Transformation of Sweet Orange</title><author>Fleming, G. H. ; O. Olivares-Fuster ; S. Fatta Del-Bosco ; J. W. Grosser</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j260t-8d486c3a758585962d01e6b99314bd0882708100dcf118dde6db4317d42d03833</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Agrobacterium</topic><topic>Agronomy. Soil science and plant productions</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Biotechnology/Genetic Transformation/Functional Genomics</topic><topic>Callus</topic><topic>Citrus sinensis</topic><topic>DNA</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic engineering</topic><topic>Genetic engineering applications</topic><topic>Genetic technics</topic><topic>Genetic transformation</topic><topic>Genetics and breeding of economic plants</topic><topic>green fluorescent protein</topic><topic>Methods. Procedures. Technologies</topic><topic>Plant breeding: fundamental aspects and methodology</topic><topic>Plants</topic><topic>Plasmids</topic><topic>polyethylene glycol</topic><topic>Protoplast culture</topic><topic>Protoplasts</topic><topic>Seedlings</topic><topic>Somatic embryos</topic><topic>Transgenic animals and transgenic plants</topic><topic>Transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fleming, G. H.</creatorcontrib><creatorcontrib>O. Olivares-Fuster</creatorcontrib><creatorcontrib>S. Fatta Del-Bosco</creatorcontrib><creatorcontrib>J. W. Grosser</creatorcontrib><collection>Pascal-Francis</collection><collection>ProQuest Central (Corporate)</collection><collection>Docstoc</collection><collection>University Readers</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>In vitro cellular & developmental biology. Plant</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fleming, G. H.</au><au>O. Olivares-Fuster</au><au>S. Fatta Del-Bosco</au><au>J. W. Grosser</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An Alternative Method for the Genetic Transformation of Sweet Orange</atitle><jtitle>In vitro cellular & developmental biology. Plant</jtitle><date>2000-11-01</date><risdate>2000</risdate><volume>36</volume><issue>6</issue><spage>450</spage><epage>455</epage><pages>450-455</pages><issn>1054-5476</issn><eissn>1475-2689</eissn><abstract>An alternative method for transforming sweet orange [Citrus sinensis (L.) Osbeck] has been developed. Plasmid DNA encoding the non-destructive selectable marker enhanced green fluorescent protein gene was introduced using polyethylene glycol into protoplasts of 'Itaborai' sweet orange isolated from an embryogenic nucellar-derived suspension culture. Following protoplast culture in liquid medium and transfer to solid medium, transformed calluses were identified via expression of the green fluorescent protein, physically separated from non-transformed tissue, and cultured on somatic embryogenesis induction medium. Transgenic plantlets were recovered from germinating somatic embryos and by in vitro rooting of shoots. To expedite transgenic plant recovery, regenerated shoots were also micrografted onto sour orange seedling rootstocks. Presence of the transgene in calluses and regenerated sweet orange plants was verified by gene amplification and Southern analyses. Potential advantages of this transformation system over the commonly used Agrobacterium methods for citrus are discussed.</abstract><cop>Wallingford</cop><pub>CABI Publishing</pub><doi>10.1007/s11627-000-0081-8</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1054-5476 |
| ispartof | In vitro cellular & developmental biology. Plant, 2000-11, Vol.36 (6), p.450-455 |
| issn | 1054-5476 1475-2689 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_17860782 |
| source | Jstor Complete Legacy; SpringerLink Journals |
| subjects | Agrobacterium Agronomy. Soil science and plant productions Biological and medical sciences Biotechnology Biotechnology/Genetic Transformation/Functional Genomics Callus Citrus sinensis DNA Fundamental and applied biological sciences. Psychology Genetic engineering Genetic engineering applications Genetic technics Genetic transformation Genetics and breeding of economic plants green fluorescent protein Methods. Procedures. Technologies Plant breeding: fundamental aspects and methodology Plants Plasmids polyethylene glycol Protoplast culture Protoplasts Seedlings Somatic embryos Transgenic animals and transgenic plants Transgenic plants |
| title | An Alternative Method for the Genetic Transformation of Sweet Orange |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T02%3A26%3A10IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=An%20Alternative%20Method%20for%20the%20Genetic%20Transformation%20of%20Sweet%20Orange&rft.jtitle=In%20vitro%20cellular%20&%20developmental%20biology.%20Plant&rft.au=Fleming,%20G.%20H.&rft.date=2000-11-01&rft.volume=36&rft.issue=6&rft.spage=450&rft.epage=455&rft.pages=450-455&rft.issn=1054-5476&rft.eissn=1475-2689&rft_id=info:doi/10.1007/s11627-000-0081-8&rft_dat=%3Cjstor_proqu%3E4293388%3C/jstor_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=197395626&rft_id=info:pmid/&rft_jstor_id=4293388&rfr_iscdi=true |