Comparison of HIV-specific CD8 T-cell responses among uninfected individuals exposed to HIV parenterally and mucosally
To assess the influence of route of HIV exposure on the development of HIV-specific CD8 T-cell responses in exposed, uninfected (EU) individuals. Two groups of EU exposed to virus through either sexual or intravenous contact were studied. Group I included subjects (n = 20) who had unprotected sexual...
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Veröffentlicht in: | AIDS (London) 2005-02, Vol.19 (3), p.251-259 |
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creator | Makedonas, George Bruneau, Julie Alary, Michel Tsoukas, Christos M Lowndes, Catherine M Lamothe, François Bernard, Nicole F |
description | To assess the influence of route of HIV exposure on the development of HIV-specific CD8 T-cell responses in exposed, uninfected (EU) individuals.
Two groups of EU exposed to virus through either sexual or intravenous contact were studied. Group I included subjects (n = 20) who had unprotected sexual contact with known HIV-infected partners and no intravenous HIV exposure; Group II included individuals (n = 27) who had shared needles with HIV-infected partners and had no sexual exposure to this virus. Between-group comparisons were made for the proportion of responders, breadth, magnitude, and specificity of HIV-specific responses.
: The interferon-gamma ELISPOT assay was used to detect HIV-specific effector activity. Peripheral blood mononuclear cells (PBMC) from each subject were stimulated with a panel of HIV peptides restricted to the MHC class I alleles expressed by the individual.
A similar proportion of EU tested from each group (35.0% Group I versus 22.2% Group II) recognized at least one HIV peptide. Group I and II subjects recognized HIV peptides with a similar cumulative intensity of 130 +/- 67.5 and 182.9 +/- 184.2 spot forming cells/1 x 10 PBMC, respectively, and similar magnitude per stimulatory peptide of 82.7 and 78.4 SFC/1 x 10 PBMC, respectively. The proportion of stimulatory peptides derived from HIV Gag, reverse transcriptase, Env, and Nef was not significantly different between the two EU groups. HLA-A*0201 restricted HIV epitopes immunodominant in infected individuals are rarely stimulatory in EU subjects.
Both mucosal and parenteral exposure to HIV can elicit HIV-specific CD8 T-cell responses with similar characteristics. |
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Two groups of EU exposed to virus through either sexual or intravenous contact were studied. Group I included subjects (n = 20) who had unprotected sexual contact with known HIV-infected partners and no intravenous HIV exposure; Group II included individuals (n = 27) who had shared needles with HIV-infected partners and had no sexual exposure to this virus. Between-group comparisons were made for the proportion of responders, breadth, magnitude, and specificity of HIV-specific responses.
: The interferon-gamma ELISPOT assay was used to detect HIV-specific effector activity. Peripheral blood mononuclear cells (PBMC) from each subject were stimulated with a panel of HIV peptides restricted to the MHC class I alleles expressed by the individual.
A similar proportion of EU tested from each group (35.0% Group I versus 22.2% Group II) recognized at least one HIV peptide. Group I and II subjects recognized HIV peptides with a similar cumulative intensity of 130 +/- 67.5 and 182.9 +/- 184.2 spot forming cells/1 x 10 PBMC, respectively, and similar magnitude per stimulatory peptide of 82.7 and 78.4 SFC/1 x 10 PBMC, respectively. The proportion of stimulatory peptides derived from HIV Gag, reverse transcriptase, Env, and Nef was not significantly different between the two EU groups. HLA-A*0201 restricted HIV epitopes immunodominant in infected individuals are rarely stimulatory in EU subjects.
Both mucosal and parenteral exposure to HIV can elicit HIV-specific CD8 T-cell responses with similar characteristics.</description><identifier>ISSN: 0269-9370</identifier><identifier>PMID: 15718835</identifier><language>eng</language><publisher>England</publisher><subject>Adult ; Amino Acid Sequence ; CD8-Positive T-Lymphocytes - immunology ; Enzyme-Linked Immunosorbent Assay - methods ; Female ; Histocompatibility Testing ; HIV Infections - immunology ; HIV Infections - transmission ; HIV-1 ; HLA-A Antigens - immunology ; HLA-A2 Antigen - immunology ; Human immunodeficiency virus ; Humans ; Immunity, Cellular ; Immunity, Mucosal ; Immunodominant Epitopes - immunology ; Interferon-gamma - biosynthesis ; Male ; Middle Aged ; Molecular Sequence Data ; Needle Sharing - adverse effects ; Sexually Transmitted Diseases, Viral - immunology ; Substance Abuse, Intravenous - complications ; Viral Proteins - genetics ; Viral Proteins - immunology</subject><ispartof>AIDS (London), 2005-02, Vol.19 (3), p.251-259</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15718835$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Makedonas, George</creatorcontrib><creatorcontrib>Bruneau, Julie</creatorcontrib><creatorcontrib>Alary, Michel</creatorcontrib><creatorcontrib>Tsoukas, Christos M</creatorcontrib><creatorcontrib>Lowndes, Catherine M</creatorcontrib><creatorcontrib>Lamothe, François</creatorcontrib><creatorcontrib>Bernard, Nicole F</creatorcontrib><title>Comparison of HIV-specific CD8 T-cell responses among uninfected individuals exposed to HIV parenterally and mucosally</title><title>AIDS (London)</title><addtitle>AIDS</addtitle><description>To assess the influence of route of HIV exposure on the development of HIV-specific CD8 T-cell responses in exposed, uninfected (EU) individuals.
Two groups of EU exposed to virus through either sexual or intravenous contact were studied. Group I included subjects (n = 20) who had unprotected sexual contact with known HIV-infected partners and no intravenous HIV exposure; Group II included individuals (n = 27) who had shared needles with HIV-infected partners and had no sexual exposure to this virus. Between-group comparisons were made for the proportion of responders, breadth, magnitude, and specificity of HIV-specific responses.
: The interferon-gamma ELISPOT assay was used to detect HIV-specific effector activity. Peripheral blood mononuclear cells (PBMC) from each subject were stimulated with a panel of HIV peptides restricted to the MHC class I alleles expressed by the individual.
A similar proportion of EU tested from each group (35.0% Group I versus 22.2% Group II) recognized at least one HIV peptide. Group I and II subjects recognized HIV peptides with a similar cumulative intensity of 130 +/- 67.5 and 182.9 +/- 184.2 spot forming cells/1 x 10 PBMC, respectively, and similar magnitude per stimulatory peptide of 82.7 and 78.4 SFC/1 x 10 PBMC, respectively. The proportion of stimulatory peptides derived from HIV Gag, reverse transcriptase, Env, and Nef was not significantly different between the two EU groups. HLA-A*0201 restricted HIV epitopes immunodominant in infected individuals are rarely stimulatory in EU subjects.
Both mucosal and parenteral exposure to HIV can elicit HIV-specific CD8 T-cell responses with similar characteristics.</description><subject>Adult</subject><subject>Amino Acid Sequence</subject><subject>CD8-Positive T-Lymphocytes - immunology</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Female</subject><subject>Histocompatibility Testing</subject><subject>HIV Infections - immunology</subject><subject>HIV Infections - transmission</subject><subject>HIV-1</subject><subject>HLA-A Antigens - immunology</subject><subject>HLA-A2 Antigen - immunology</subject><subject>Human immunodeficiency virus</subject><subject>Humans</subject><subject>Immunity, Cellular</subject><subject>Immunity, Mucosal</subject><subject>Immunodominant Epitopes - immunology</subject><subject>Interferon-gamma - biosynthesis</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Molecular Sequence Data</subject><subject>Needle Sharing - adverse effects</subject><subject>Sexually Transmitted Diseases, Viral - immunology</subject><subject>Substance Abuse, Intravenous - complications</subject><subject>Viral Proteins - genetics</subject><subject>Viral Proteins - immunology</subject><issn>0269-9370</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kMtOwzAQRb0A0VL4BeQVu0h2_MwShUeRKrEpbCPHniCjxDZxUtG_pxFlNZrRuUeae4HWpJRVUTFFVug65y9CiCBaX6EVFYpqzcQaHeo4JDP6HAOOHd6-fhQ5gfWdt7h-1HhfWOh7PEJOMWTI2AwxfOI5-NCBncBhH5w_eDebPmP4STGfblNcTPgkhjDBaPr-iE1weJhtzMt2gy67UwBuz3OD3p-f9vW22L29vNYPuyKVnEyFlK51UuoSSqOYUrwVTHRSVkRzrYRl2gApK112reZOcwmMkpYS2lFhBeVsg-7_vGmM3zPkqRl8Xj4yAeKcG6q0JLxawLszOLcDuCaNfjDjsflviv0CJ5Zj_g</recordid><startdate>20050218</startdate><enddate>20050218</enddate><creator>Makedonas, George</creator><creator>Bruneau, Julie</creator><creator>Alary, Michel</creator><creator>Tsoukas, Christos M</creator><creator>Lowndes, Catherine M</creator><creator>Lamothe, François</creator><creator>Bernard, Nicole F</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>20050218</creationdate><title>Comparison of HIV-specific CD8 T-cell responses among uninfected individuals exposed to HIV parenterally and mucosally</title><author>Makedonas, George ; Bruneau, Julie ; Alary, Michel ; Tsoukas, Christos M ; Lowndes, Catherine M ; Lamothe, François ; Bernard, Nicole F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p240t-66dbd6682e2a73774b535f669084875c38ae02982fb84d846e310b101f15c5143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Adult</topic><topic>Amino Acid Sequence</topic><topic>CD8-Positive T-Lymphocytes - immunology</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Female</topic><topic>Histocompatibility Testing</topic><topic>HIV Infections - immunology</topic><topic>HIV Infections - transmission</topic><topic>HIV-1</topic><topic>HLA-A Antigens - immunology</topic><topic>HLA-A2 Antigen - immunology</topic><topic>Human immunodeficiency virus</topic><topic>Humans</topic><topic>Immunity, Cellular</topic><topic>Immunity, Mucosal</topic><topic>Immunodominant Epitopes - immunology</topic><topic>Interferon-gamma - biosynthesis</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Molecular Sequence Data</topic><topic>Needle Sharing - adverse effects</topic><topic>Sexually Transmitted Diseases, Viral - immunology</topic><topic>Substance Abuse, Intravenous - complications</topic><topic>Viral Proteins - genetics</topic><topic>Viral Proteins - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Makedonas, George</creatorcontrib><creatorcontrib>Bruneau, Julie</creatorcontrib><creatorcontrib>Alary, Michel</creatorcontrib><creatorcontrib>Tsoukas, Christos M</creatorcontrib><creatorcontrib>Lowndes, Catherine M</creatorcontrib><creatorcontrib>Lamothe, François</creatorcontrib><creatorcontrib>Bernard, Nicole F</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>AIDS (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Makedonas, George</au><au>Bruneau, Julie</au><au>Alary, Michel</au><au>Tsoukas, Christos M</au><au>Lowndes, Catherine M</au><au>Lamothe, François</au><au>Bernard, Nicole F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of HIV-specific CD8 T-cell responses among uninfected individuals exposed to HIV parenterally and mucosally</atitle><jtitle>AIDS (London)</jtitle><addtitle>AIDS</addtitle><date>2005-02-18</date><risdate>2005</risdate><volume>19</volume><issue>3</issue><spage>251</spage><epage>259</epage><pages>251-259</pages><issn>0269-9370</issn><abstract>To assess the influence of route of HIV exposure on the development of HIV-specific CD8 T-cell responses in exposed, uninfected (EU) individuals.
Two groups of EU exposed to virus through either sexual or intravenous contact were studied. Group I included subjects (n = 20) who had unprotected sexual contact with known HIV-infected partners and no intravenous HIV exposure; Group II included individuals (n = 27) who had shared needles with HIV-infected partners and had no sexual exposure to this virus. Between-group comparisons were made for the proportion of responders, breadth, magnitude, and specificity of HIV-specific responses.
: The interferon-gamma ELISPOT assay was used to detect HIV-specific effector activity. Peripheral blood mononuclear cells (PBMC) from each subject were stimulated with a panel of HIV peptides restricted to the MHC class I alleles expressed by the individual.
A similar proportion of EU tested from each group (35.0% Group I versus 22.2% Group II) recognized at least one HIV peptide. Group I and II subjects recognized HIV peptides with a similar cumulative intensity of 130 +/- 67.5 and 182.9 +/- 184.2 spot forming cells/1 x 10 PBMC, respectively, and similar magnitude per stimulatory peptide of 82.7 and 78.4 SFC/1 x 10 PBMC, respectively. The proportion of stimulatory peptides derived from HIV Gag, reverse transcriptase, Env, and Nef was not significantly different between the two EU groups. HLA-A*0201 restricted HIV epitopes immunodominant in infected individuals are rarely stimulatory in EU subjects.
Both mucosal and parenteral exposure to HIV can elicit HIV-specific CD8 T-cell responses with similar characteristics.</abstract><cop>England</cop><pmid>15718835</pmid><tpages>9</tpages></addata></record> |
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source | MEDLINE; Journals@Ovid Complete; EZB-FREE-00999 freely available EZB journals |
subjects | Adult Amino Acid Sequence CD8-Positive T-Lymphocytes - immunology Enzyme-Linked Immunosorbent Assay - methods Female Histocompatibility Testing HIV Infections - immunology HIV Infections - transmission HIV-1 HLA-A Antigens - immunology HLA-A2 Antigen - immunology Human immunodeficiency virus Humans Immunity, Cellular Immunity, Mucosal Immunodominant Epitopes - immunology Interferon-gamma - biosynthesis Male Middle Aged Molecular Sequence Data Needle Sharing - adverse effects Sexually Transmitted Diseases, Viral - immunology Substance Abuse, Intravenous - complications Viral Proteins - genetics Viral Proteins - immunology |
title | Comparison of HIV-specific CD8 T-cell responses among uninfected individuals exposed to HIV parenterally and mucosally |
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