Comparison of HIV-specific CD8 T-cell responses among uninfected individuals exposed to HIV parenterally and mucosally

To assess the influence of route of HIV exposure on the development of HIV-specific CD8 T-cell responses in exposed, uninfected (EU) individuals. Two groups of EU exposed to virus through either sexual or intravenous contact were studied. Group I included subjects (n = 20) who had unprotected sexual...

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Veröffentlicht in:AIDS (London) 2005-02, Vol.19 (3), p.251-259
Hauptverfasser: Makedonas, George, Bruneau, Julie, Alary, Michel, Tsoukas, Christos M, Lowndes, Catherine M, Lamothe, François, Bernard, Nicole F
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container_end_page 259
container_issue 3
container_start_page 251
container_title AIDS (London)
container_volume 19
creator Makedonas, George
Bruneau, Julie
Alary, Michel
Tsoukas, Christos M
Lowndes, Catherine M
Lamothe, François
Bernard, Nicole F
description To assess the influence of route of HIV exposure on the development of HIV-specific CD8 T-cell responses in exposed, uninfected (EU) individuals. Two groups of EU exposed to virus through either sexual or intravenous contact were studied. Group I included subjects (n = 20) who had unprotected sexual contact with known HIV-infected partners and no intravenous HIV exposure; Group II included individuals (n = 27) who had shared needles with HIV-infected partners and had no sexual exposure to this virus. Between-group comparisons were made for the proportion of responders, breadth, magnitude, and specificity of HIV-specific responses. : The interferon-gamma ELISPOT assay was used to detect HIV-specific effector activity. Peripheral blood mononuclear cells (PBMC) from each subject were stimulated with a panel of HIV peptides restricted to the MHC class I alleles expressed by the individual. A similar proportion of EU tested from each group (35.0% Group I versus 22.2% Group II) recognized at least one HIV peptide. Group I and II subjects recognized HIV peptides with a similar cumulative intensity of 130 +/- 67.5 and 182.9 +/- 184.2 spot forming cells/1 x 10 PBMC, respectively, and similar magnitude per stimulatory peptide of 82.7 and 78.4 SFC/1 x 10 PBMC, respectively. The proportion of stimulatory peptides derived from HIV Gag, reverse transcriptase, Env, and Nef was not significantly different between the two EU groups. HLA-A*0201 restricted HIV epitopes immunodominant in infected individuals are rarely stimulatory in EU subjects. Both mucosal and parenteral exposure to HIV can elicit HIV-specific CD8 T-cell responses with similar characteristics.
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subjects Adult
Amino Acid Sequence
CD8-Positive T-Lymphocytes - immunology
Enzyme-Linked Immunosorbent Assay - methods
Female
Histocompatibility Testing
HIV Infections - immunology
HIV Infections - transmission
HIV-1
HLA-A Antigens - immunology
HLA-A2 Antigen - immunology
Human immunodeficiency virus
Humans
Immunity, Cellular
Immunity, Mucosal
Immunodominant Epitopes - immunology
Interferon-gamma - biosynthesis
Male
Middle Aged
Molecular Sequence Data
Needle Sharing - adverse effects
Sexually Transmitted Diseases, Viral - immunology
Substance Abuse, Intravenous - complications
Viral Proteins - genetics
Viral Proteins - immunology
title Comparison of HIV-specific CD8 T-cell responses among uninfected individuals exposed to HIV parenterally and mucosally
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