Enterococcus faecalis multi-drug resistance transporters: application for antibiotic discovery
Using bioinformatics approaches, 34 potential multidrug resistance (MDR) transporter sequences representing 4 different transporter families were identified in the unannotated Enterococcus faecalis database (TIGR). A functional genomics campaign generating single-gene insertional disruptions reveale...
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| Veröffentlicht in: | Journal of molecular microbiology and biotechnology 2001-04, Vol.3 (2), p.179-184 |
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| container_title | Journal of molecular microbiology and biotechnology |
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| creator | Davis, D R McAlpine, J B Pazoles, C J Talbot, M K Alder, E A White, C Jonas, B M Murray, B E Weinstock, G M Rogers, B L |
| description | Using bioinformatics approaches, 34 potential multidrug resistance (MDR) transporter sequences representing 4 different transporter families were identified in the unannotated Enterococcus faecalis database (TIGR). A functional genomics campaign generating single-gene insertional disruptions revealed several genes whose absence confers significant hypersensitivities to known antimicrobials. We constructed specific strains, disrupted in a variety of previously unpublished, putative MDR transporter genes, as tools to improve the success of whole-cell antimicrobial screening and discovery. Each of the potential transporters was inactivated at the gene level and then phenotypically characterized, both with single disruption mutants and with 2-gene mutants built upon a delta norA deleted strain background. |
| format | Article |
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A functional genomics campaign generating single-gene insertional disruptions revealed several genes whose absence confers significant hypersensitivities to known antimicrobials. We constructed specific strains, disrupted in a variety of previously unpublished, putative MDR transporter genes, as tools to improve the success of whole-cell antimicrobial screening and discovery. Each of the potential transporters was inactivated at the gene level and then phenotypically characterized, both with single disruption mutants and with 2-gene mutants built upon a delta norA deleted strain background.</description><identifier>ISSN: 1464-1801</identifier><identifier>PMID: 11321571</identifier><language>eng</language><publisher>Switzerland</publisher><subject>Anti-Bacterial Agents - chemical synthesis ; Anti-Bacterial Agents - chemistry ; Anti-Bacterial Agents - pharmacology ; ATP-Binding Cassette Transporters - genetics ; ATP-Binding Cassette Transporters - metabolism ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Carrier Proteins - genetics ; Carrier Proteins - metabolism ; Databases as Topic ; Drug Design ; Drug Resistance, Microbial ; Enterococcus faecalis ; Enterococcus faecalis - drug effects ; Enterococcus faecalis - genetics ; Enterococcus faecalis - physiology ; Gene Deletion ; Genomics ; Multidrug Resistance-Associated Proteins ; Mutagenesis ; norA gene ; Structure-Activity Relationship</subject><ispartof>Journal of molecular microbiology and biotechnology, 2001-04, Vol.3 (2), p.179-184</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11321571$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Davis, D R</creatorcontrib><creatorcontrib>McAlpine, J B</creatorcontrib><creatorcontrib>Pazoles, C J</creatorcontrib><creatorcontrib>Talbot, M K</creatorcontrib><creatorcontrib>Alder, E A</creatorcontrib><creatorcontrib>White, C</creatorcontrib><creatorcontrib>Jonas, B M</creatorcontrib><creatorcontrib>Murray, B E</creatorcontrib><creatorcontrib>Weinstock, G M</creatorcontrib><creatorcontrib>Rogers, B L</creatorcontrib><title>Enterococcus faecalis multi-drug resistance transporters: application for antibiotic discovery</title><title>Journal of molecular microbiology and biotechnology</title><addtitle>J Mol Microbiol Biotechnol</addtitle><description>Using bioinformatics approaches, 34 potential multidrug resistance (MDR) transporter sequences representing 4 different transporter families were identified in the unannotated Enterococcus faecalis database (TIGR). 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| ispartof | Journal of molecular microbiology and biotechnology, 2001-04, Vol.3 (2), p.179-184 |
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| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Anti-Bacterial Agents - chemical synthesis Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - pharmacology ATP-Binding Cassette Transporters - genetics ATP-Binding Cassette Transporters - metabolism Bacterial Proteins - genetics Bacterial Proteins - metabolism Carrier Proteins - genetics Carrier Proteins - metabolism Databases as Topic Drug Design Drug Resistance, Microbial Enterococcus faecalis Enterococcus faecalis - drug effects Enterococcus faecalis - genetics Enterococcus faecalis - physiology Gene Deletion Genomics Multidrug Resistance-Associated Proteins Mutagenesis norA gene Structure-Activity Relationship |
| title | Enterococcus faecalis multi-drug resistance transporters: application for antibiotic discovery |
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