High Frequency Plant Regeneration via Somatic Embryogenesis in Cell Suspension Cultures of Cowpea, Vigna unguiculata (L.) Walp
Embryogenic callus was induced from primary leaves of Vigna unguiculata (L.) Walp. in MS medium (Murashige and Skoog, 1962) containing 2,4-dichlorophenoxyacetic acid (2,4-D). Greenish-white, friable embryogenic calluses were used to establish suspension cultures. A shaking speed of 90 rpm and 0.4 ml...
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| Veröffentlicht in: | In vitro cellular & developmental biology. Plant 2000-11, Vol.36 (6), p.475-480 |
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| container_title | In vitro cellular & developmental biology. Plant |
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| creator | PREM ANAND, R GANAPATHI, A RAMESH ANBAZHAGAN, V VENGADESAN, G SELVARAJ, N |
| description | Embryogenic callus was induced from primary leaves of Vigna unguiculata (L.) Walp. in MS medium (Murashige and Skoog, 1962) containing 2,4-dichlorophenoxyacetic acid (2,4-D). Greenish-white, friable embryogenic calluses were used to establish suspension cultures. A shaking speed of 90 rpm and 0.4 ml packed cell volume per 25 ml medium were found to be optimal for maintaining suspension cultures. Globular, heart-shaped and torpedo-shaped embryos were developed in suspension culture containing 4.52 µM 2,4-D. Maturation of cotyledonary-stage somatic embryos was achieved on 0.05 µM 2,4-D, 5 µM abscisic acid and 3% mannitol. Twenty-two percent of the embryos were converted into plants and survived; survival in the field was 8-10%. |
| doi_str_mv | 10.1007/s11627-000-0085-4 |
| format | Article |
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A shaking speed of 90 rpm and 0.4 ml packed cell volume per 25 ml medium were found to be optimal for maintaining suspension cultures. Globular, heart-shaped and torpedo-shaped embryos were developed in suspension culture containing 4.52 µM 2,4-D. Maturation of cotyledonary-stage somatic embryos was achieved on 0.05 µM 2,4-D, 5 µM abscisic acid and 3% mannitol. Twenty-two percent of the embryos were converted into plants and survived; survival in the field was 8-10%.</abstract><cop>Wallingford</cop><pub>CABI Publishing</pub><doi>10.1007/s11627-000-0085-4</doi><tpages>6</tpages></addata></record> |
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| ispartof | In vitro cellular & developmental biology. Plant, 2000-11, Vol.36 (6), p.475-480 |
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| language | eng |
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| source | SpringerNature Journals; JSTOR Archive Collection A-Z Listing |
| subjects | 2,4-D Biological and medical sciences Biotechnology Callus Chemical suspensions Cultured cells Developmental Biology/Morphogenesis Embryonic cells Embryonic growth stage Embryos Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology In vitro propagation: entire plant regeneration from tissues and cell cultures Liquids Methods. Procedures. Technologies Plant cells Plant cells and fungal cells Plants Somatic embryogenesis Somatic embryos Vigna unguiculata |
| title | High Frequency Plant Regeneration via Somatic Embryogenesis in Cell Suspension Cultures of Cowpea, Vigna unguiculata (L.) Walp |
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