Combination of anti-tuberculosis drugs with vitamin C or NAC against different Staphylococcus aureus and Mycobacterium tuberculosis strains
Hepatotoxicity due to anti tuberculosis drugs, rifampin and isoniazid, is a major problem in tuberculosis patients. Vitamin C, an antioxidant, and N-acetyl cysteine (NAC), a scavenger of active metabolites, reduce the hepatotoxicity. The aim of present study was to investigate the effect of vitamin...
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Veröffentlicht in: | Microbial pathogenesis 2016-04, Vol.93, p.83-87 |
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description | Hepatotoxicity due to anti tuberculosis drugs, rifampin and isoniazid, is a major problem in tuberculosis patients. Vitamin C, an antioxidant, and N-acetyl cysteine (NAC), a scavenger of active metabolites, reduce the hepatotoxicity. The aim of present study was to investigate the effect of vitamin C and NAC individually on the antibacterial activity of anti tuberculosis drugs against Mycobacterium tuberculosis and Staphylococcus aureus strains.
The MICs of each compound against all strains were determined in 96 wells plate. Rifampin was tested at serial two fold concentrations alone or in combination with NAC or vitamin C.
The MIC of rifampin against different strains of S. aureus was 0.008–0.032 μg/ml. The MIC of rifampin and isoniazid against M. tuberculosis strains were 40 and 0.2 μg/ml, respectively. Vitamin C and NAC had no antibacterial activity against all strains. MIC of rifampin was reduced two fold by combination with vitamin C for all S. aureus strains, while NAC did not affect the antibacterial activity of rifampin. Vitamin C and NAC had remarkable effects on the antibacterial activity of anti-tuberculosis drugs against M. tuberculosis.
Synergistic effects were observed between rifampin or isoniazid and vitamin C against all tested strains. However, combination therapy of rifampin and isoniazid with NAC was not being effective. This study highlighted the advantages of combination of anti-tuberculosis drugs and vitamin C to eradicate the microbial infections.
•Combination therapy of rifampin with vitamin C was effective approach to combat staphylococcal infections.•Synergistic effects were observed between rifampin or isoniazid and vitamin C against Mycobacterium tuberculosis strains.•NAC did not affect the antibacterial activity of anti-tuberculosis drugs. |
doi_str_mv | 10.1016/j.micpath.2015.11.006 |
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The MICs of each compound against all strains were determined in 96 wells plate. Rifampin was tested at serial two fold concentrations alone or in combination with NAC or vitamin C.
The MIC of rifampin against different strains of S. aureus was 0.008–0.032 μg/ml. The MIC of rifampin and isoniazid against M. tuberculosis strains were 40 and 0.2 μg/ml, respectively. Vitamin C and NAC had no antibacterial activity against all strains. MIC of rifampin was reduced two fold by combination with vitamin C for all S. aureus strains, while NAC did not affect the antibacterial activity of rifampin. Vitamin C and NAC had remarkable effects on the antibacterial activity of anti-tuberculosis drugs against M. tuberculosis.
Synergistic effects were observed between rifampin or isoniazid and vitamin C against all tested strains. However, combination therapy of rifampin and isoniazid with NAC was not being effective. This study highlighted the advantages of combination of anti-tuberculosis drugs and vitamin C to eradicate the microbial infections.
•Combination therapy of rifampin with vitamin C was effective approach to combat staphylococcal infections.•Synergistic effects were observed between rifampin or isoniazid and vitamin C against Mycobacterium tuberculosis strains.•NAC did not affect the antibacterial activity of anti-tuberculosis drugs.</description><identifier>ISSN: 0882-4010</identifier><identifier>EISSN: 1096-1208</identifier><identifier>DOI: 10.1016/j.micpath.2015.11.006</identifier><identifier>PMID: 26602814</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Acetylcysteine - pharmacology ; Antitubercular Agents - pharmacology ; Ascorbic Acid - pharmacology ; Drug Synergism ; Drug Therapy, Combination ; Humans ; Isoniazid ; Isoniazid - pharmacology ; Microbial Sensitivity Tests ; Mycobacterium tuberculosis ; Mycobacterium tuberculosis - drug effects ; Mycobacterium tuberculosis - physiology ; N-acetyl cysteine ; Rifampin ; Rifampin - pharmacology ; Staphylococcal Infections - drug therapy ; Staphylococcal Infections - microbiology ; Staphylococcus aureus ; Staphylococcus aureus - drug effects ; Staphylococcus aureus - physiology ; Tuberculosis - drug therapy ; Tuberculosis - microbiology ; Vitamin C</subject><ispartof>Microbial pathogenesis, 2016-04, Vol.93, p.83-87</ispartof><rights>2016 Elsevier Ltd</rights><rights>Copyright © 2016 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c398t-624816e88b276d483b814b9ccc442f555620474b0c09659c03283c6be1aa9ffa3</citedby><cites>FETCH-LOGICAL-c398t-624816e88b276d483b814b9ccc442f555620474b0c09659c03283c6be1aa9ffa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.micpath.2015.11.006$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26602814$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Khameneh, Bahman</creatorcontrib><creatorcontrib>Fazly Bazzaz, Bibi Sedigheh</creatorcontrib><creatorcontrib>Amani, Alireza</creatorcontrib><creatorcontrib>Rostami, Javad</creatorcontrib><creatorcontrib>Vahdati-Mashhadian, Nasser</creatorcontrib><title>Combination of anti-tuberculosis drugs with vitamin C or NAC against different Staphylococcus aureus and Mycobacterium tuberculosis strains</title><title>Microbial pathogenesis</title><addtitle>Microb Pathog</addtitle><description>Hepatotoxicity due to anti tuberculosis drugs, rifampin and isoniazid, is a major problem in tuberculosis patients. Vitamin C, an antioxidant, and N-acetyl cysteine (NAC), a scavenger of active metabolites, reduce the hepatotoxicity. The aim of present study was to investigate the effect of vitamin C and NAC individually on the antibacterial activity of anti tuberculosis drugs against Mycobacterium tuberculosis and Staphylococcus aureus strains.
The MICs of each compound against all strains were determined in 96 wells plate. Rifampin was tested at serial two fold concentrations alone or in combination with NAC or vitamin C.
The MIC of rifampin against different strains of S. aureus was 0.008–0.032 μg/ml. The MIC of rifampin and isoniazid against M. tuberculosis strains were 40 and 0.2 μg/ml, respectively. Vitamin C and NAC had no antibacterial activity against all strains. MIC of rifampin was reduced two fold by combination with vitamin C for all S. aureus strains, while NAC did not affect the antibacterial activity of rifampin. Vitamin C and NAC had remarkable effects on the antibacterial activity of anti-tuberculosis drugs against M. tuberculosis.
Synergistic effects were observed between rifampin or isoniazid and vitamin C against all tested strains. However, combination therapy of rifampin and isoniazid with NAC was not being effective. This study highlighted the advantages of combination of anti-tuberculosis drugs and vitamin C to eradicate the microbial infections.
•Combination therapy of rifampin with vitamin C was effective approach to combat staphylococcal infections.•Synergistic effects were observed between rifampin or isoniazid and vitamin C against Mycobacterium tuberculosis strains.•NAC did not affect the antibacterial activity of anti-tuberculosis drugs.</description><subject>Acetylcysteine - pharmacology</subject><subject>Antitubercular Agents - pharmacology</subject><subject>Ascorbic Acid - pharmacology</subject><subject>Drug Synergism</subject><subject>Drug Therapy, Combination</subject><subject>Humans</subject><subject>Isoniazid</subject><subject>Isoniazid - pharmacology</subject><subject>Microbial Sensitivity Tests</subject><subject>Mycobacterium tuberculosis</subject><subject>Mycobacterium tuberculosis - drug effects</subject><subject>Mycobacterium tuberculosis - physiology</subject><subject>N-acetyl cysteine</subject><subject>Rifampin</subject><subject>Rifampin - pharmacology</subject><subject>Staphylococcal Infections - drug therapy</subject><subject>Staphylococcal Infections - microbiology</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Staphylococcus aureus - physiology</subject><subject>Tuberculosis - drug therapy</subject><subject>Tuberculosis - microbiology</subject><subject>Vitamin C</subject><issn>0882-4010</issn><issn>1096-1208</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUcuO0zAUtRCIKQOfAPKSTcK1k7jOCo2i4SENsADWln3jTF0ldvFjUL-BnyZVCxIrVmdzHjrnEPKSQc2AiTf7enF40HlXc2BdzVgNIB6RDYNeVIyDfEw2ICWvWmBwRZ6ltAeAvm36p-SKCwFcsnZDfg1hMc7r7IKnYaLaZ1flYmzEMofkEh1juU_0p8s7-uCyXpynAw2Rfr4ZqL7XzqdMRzdNNlqf6desD7vjHDAglkR1ifYEfqSfjhiMxmyjKwv9JyLlePJ5Tp5Mek72xQWvyfd3t9-GD9Xdl_cfh5u7Cpte5krwVjJhpTR8K8ZWNmZtYnpEbFs-dV0nOLTb1gCuU3Q9QsNlg8JYpnU_Tbq5Jq_PvocYfhSbslpcQjvP2ttQkmJb2fHVS_CV2p2pGENK0U7qEN2i41ExUKcf1F5dflCnHxRjav1h1b26RBSz2PGv6s_wK-HtmWDXog_ORpXQWY92dNFiVmNw_4n4Dbwwnqc</recordid><startdate>201604</startdate><enddate>201604</enddate><creator>Khameneh, Bahman</creator><creator>Fazly Bazzaz, Bibi Sedigheh</creator><creator>Amani, Alireza</creator><creator>Rostami, Javad</creator><creator>Vahdati-Mashhadian, Nasser</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>201604</creationdate><title>Combination of anti-tuberculosis drugs with vitamin C or NAC against different Staphylococcus aureus and Mycobacterium tuberculosis strains</title><author>Khameneh, Bahman ; Fazly Bazzaz, Bibi Sedigheh ; Amani, Alireza ; Rostami, Javad ; Vahdati-Mashhadian, Nasser</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c398t-624816e88b276d483b814b9ccc442f555620474b0c09659c03283c6be1aa9ffa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Acetylcysteine - pharmacology</topic><topic>Antitubercular Agents - pharmacology</topic><topic>Ascorbic Acid - pharmacology</topic><topic>Drug Synergism</topic><topic>Drug Therapy, Combination</topic><topic>Humans</topic><topic>Isoniazid</topic><topic>Isoniazid - pharmacology</topic><topic>Microbial Sensitivity Tests</topic><topic>Mycobacterium tuberculosis</topic><topic>Mycobacterium tuberculosis - drug effects</topic><topic>Mycobacterium tuberculosis - physiology</topic><topic>N-acetyl cysteine</topic><topic>Rifampin</topic><topic>Rifampin - pharmacology</topic><topic>Staphylococcal Infections - drug therapy</topic><topic>Staphylococcal Infections - microbiology</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - drug effects</topic><topic>Staphylococcus aureus - physiology</topic><topic>Tuberculosis - drug therapy</topic><topic>Tuberculosis - microbiology</topic><topic>Vitamin C</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Khameneh, Bahman</creatorcontrib><creatorcontrib>Fazly Bazzaz, Bibi Sedigheh</creatorcontrib><creatorcontrib>Amani, Alireza</creatorcontrib><creatorcontrib>Rostami, Javad</creatorcontrib><creatorcontrib>Vahdati-Mashhadian, Nasser</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Microbial pathogenesis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Khameneh, Bahman</au><au>Fazly Bazzaz, Bibi Sedigheh</au><au>Amani, Alireza</au><au>Rostami, Javad</au><au>Vahdati-Mashhadian, Nasser</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Combination of anti-tuberculosis drugs with vitamin C or NAC against different Staphylococcus aureus and Mycobacterium tuberculosis strains</atitle><jtitle>Microbial pathogenesis</jtitle><addtitle>Microb Pathog</addtitle><date>2016-04</date><risdate>2016</risdate><volume>93</volume><spage>83</spage><epage>87</epage><pages>83-87</pages><issn>0882-4010</issn><eissn>1096-1208</eissn><abstract>Hepatotoxicity due to anti tuberculosis drugs, rifampin and isoniazid, is a major problem in tuberculosis patients. Vitamin C, an antioxidant, and N-acetyl cysteine (NAC), a scavenger of active metabolites, reduce the hepatotoxicity. The aim of present study was to investigate the effect of vitamin C and NAC individually on the antibacterial activity of anti tuberculosis drugs against Mycobacterium tuberculosis and Staphylococcus aureus strains.
The MICs of each compound against all strains were determined in 96 wells plate. Rifampin was tested at serial two fold concentrations alone or in combination with NAC or vitamin C.
The MIC of rifampin against different strains of S. aureus was 0.008–0.032 μg/ml. The MIC of rifampin and isoniazid against M. tuberculosis strains were 40 and 0.2 μg/ml, respectively. Vitamin C and NAC had no antibacterial activity against all strains. MIC of rifampin was reduced two fold by combination with vitamin C for all S. aureus strains, while NAC did not affect the antibacterial activity of rifampin. Vitamin C and NAC had remarkable effects on the antibacterial activity of anti-tuberculosis drugs against M. tuberculosis.
Synergistic effects were observed between rifampin or isoniazid and vitamin C against all tested strains. However, combination therapy of rifampin and isoniazid with NAC was not being effective. This study highlighted the advantages of combination of anti-tuberculosis drugs and vitamin C to eradicate the microbial infections.
•Combination therapy of rifampin with vitamin C was effective approach to combat staphylococcal infections.•Synergistic effects were observed between rifampin or isoniazid and vitamin C against Mycobacterium tuberculosis strains.•NAC did not affect the antibacterial activity of anti-tuberculosis drugs.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>26602814</pmid><doi>10.1016/j.micpath.2015.11.006</doi><tpages>5</tpages></addata></record> |
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subjects | Acetylcysteine - pharmacology Antitubercular Agents - pharmacology Ascorbic Acid - pharmacology Drug Synergism Drug Therapy, Combination Humans Isoniazid Isoniazid - pharmacology Microbial Sensitivity Tests Mycobacterium tuberculosis Mycobacterium tuberculosis - drug effects Mycobacterium tuberculosis - physiology N-acetyl cysteine Rifampin Rifampin - pharmacology Staphylococcal Infections - drug therapy Staphylococcal Infections - microbiology Staphylococcus aureus Staphylococcus aureus - drug effects Staphylococcus aureus - physiology Tuberculosis - drug therapy Tuberculosis - microbiology Vitamin C |
title | Combination of anti-tuberculosis drugs with vitamin C or NAC against different Staphylococcus aureus and Mycobacterium tuberculosis strains |
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