Molecular characterization and antimicrobial susceptibility of tcdA -negative Clostridium difficile isolates from Guangzhou, China
Abstract This study aimed to investigate the molecular characteristics and antimicrobial susceptibility of Clostridium difficile clinical isolates in Guangzhou, China. One hundred twenty isolates were collected from Guangzhou General Hospital at the Guangzhou Military Command in China from March 201...
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| Veröffentlicht in: | Diagnostic microbiology and infectious disease 2016-04, Vol.84 (4), p.361-365 |
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| creator | Lidan, Chen Linhai, Li Yang, Liao Zhaohui, Sun Xiaoyan, Huang Yuling, Shi |
| description | Abstract This study aimed to investigate the molecular characteristics and antimicrobial susceptibility of Clostridium difficile clinical isolates in Guangzhou, China. One hundred twenty isolates were collected from Guangzhou General Hospital at the Guangzhou Military Command in China from March 2014 to April 2015, and 9 isolates were identified as tcdA -negative/ tcdB -positive (A− B+ ) strains. Results showed that all of the strains were confirmed to be ST37 and 0 single nucleotide variants (SNVs) were found in the PaLoc region, and >60 SNVs were identified throughout the whole genome sequence. The results show the diversity of the antibiotic and gene mutations present in these strains. All of the A− B+ isolates were highly resistant to clindamycin and erythromycin; showed an average sensitivity to fluoroquinolones; and maintained a high susceptibility to metronidazole, vancomycin, and tigecycline. |
| doi_str_mv | 10.1016/j.diagmicrobio.2015.12.015 |
| format | Article |
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One hundred twenty isolates were collected from Guangzhou General Hospital at the Guangzhou Military Command in China from March 2014 to April 2015, and 9 isolates were identified as tcdA -negative/ tcdB -positive (A− B+ ) strains. Results showed that all of the strains were confirmed to be ST37 and 0 single nucleotide variants (SNVs) were found in the PaLoc region, and >60 SNVs were identified throughout the whole genome sequence. The results show the diversity of the antibiotic and gene mutations present in these strains. All of the A− B+ isolates were highly resistant to clindamycin and erythromycin; showed an average sensitivity to fluoroquinolones; and maintained a high susceptibility to metronidazole, vancomycin, and tigecycline.</description><identifier>ISSN: 0732-8893</identifier><identifier>EISSN: 1879-0070</identifier><identifier>DOI: 10.1016/j.diagmicrobio.2015.12.015</identifier><identifier>PMID: 26867962</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adult ; Anti-Bacterial Agents - pharmacology ; Antimicrobial susceptibility test ; Bacterial Proteins - genetics ; Bacterial Toxins - genetics ; China ; Clostridium difficile ; Clostridium difficile - classification ; Clostridium difficile - drug effects ; Clostridium difficile - genetics ; Clostridium difficile - isolation & purification ; Clostridium Infections - microbiology ; Drug Resistance, Bacterial ; Enterotoxins - deficiency ; Genetic Variation ; Genome, Bacterial ; Genotype ; gyrA gene ; gyrB gene ; High-throughput sequencing ; Hospitals, General ; Humans ; Infectious Disease ; Internal Medicine ; Microbial Sensitivity Tests ; Sequence Analysis, DNA ; TcdA-negative strain</subject><ispartof>Diagnostic microbiology and infectious disease, 2016-04, Vol.84 (4), p.361-365</ispartof><rights>Elsevier Inc.</rights><rights>2016 Elsevier Inc.</rights><rights>Copyright © 2016 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c538t-bfb76deea447fec8f7972f1fdf00465116d9f766498b1da821b9634e157afa693</citedby><cites>FETCH-LOGICAL-c538t-bfb76deea447fec8f7972f1fdf00465116d9f766498b1da821b9634e157afa693</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0732889315004654$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26867962$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lidan, Chen</creatorcontrib><creatorcontrib>Linhai, Li</creatorcontrib><creatorcontrib>Yang, Liao</creatorcontrib><creatorcontrib>Zhaohui, Sun</creatorcontrib><creatorcontrib>Xiaoyan, Huang</creatorcontrib><creatorcontrib>Yuling, Shi</creatorcontrib><title>Molecular characterization and antimicrobial susceptibility of tcdA -negative Clostridium difficile isolates from Guangzhou, China</title><title>Diagnostic microbiology and infectious disease</title><addtitle>Diagn Microbiol Infect Dis</addtitle><description>Abstract This study aimed to investigate the molecular characteristics and antimicrobial susceptibility of Clostridium difficile clinical isolates in Guangzhou, China. One hundred twenty isolates were collected from Guangzhou General Hospital at the Guangzhou Military Command in China from March 2014 to April 2015, and 9 isolates were identified as tcdA -negative/ tcdB -positive (A− B+ ) strains. Results showed that all of the strains were confirmed to be ST37 and 0 single nucleotide variants (SNVs) were found in the PaLoc region, and >60 SNVs were identified throughout the whole genome sequence. The results show the diversity of the antibiotic and gene mutations present in these strains. All of the A− B+ isolates were highly resistant to clindamycin and erythromycin; showed an average sensitivity to fluoroquinolones; and maintained a high susceptibility to metronidazole, vancomycin, and tigecycline.</description><subject>Adult</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antimicrobial susceptibility test</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Toxins - genetics</subject><subject>China</subject><subject>Clostridium difficile</subject><subject>Clostridium difficile - classification</subject><subject>Clostridium difficile - drug effects</subject><subject>Clostridium difficile - genetics</subject><subject>Clostridium difficile - isolation & purification</subject><subject>Clostridium Infections - microbiology</subject><subject>Drug Resistance, Bacterial</subject><subject>Enterotoxins - deficiency</subject><subject>Genetic Variation</subject><subject>Genome, Bacterial</subject><subject>Genotype</subject><subject>gyrA gene</subject><subject>gyrB gene</subject><subject>High-throughput sequencing</subject><subject>Hospitals, General</subject><subject>Humans</subject><subject>Infectious Disease</subject><subject>Internal Medicine</subject><subject>Microbial Sensitivity Tests</subject><subject>Sequence Analysis, DNA</subject><subject>TcdA-negative strain</subject><issn>0732-8893</issn><issn>1879-0070</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUk1vEzEUXCEQDYW_gCxOHLrB9n54zQGpCrQgFXEAzpbXfk5e8K6DvVspPfLLcZqAEKcenuYy80Zv5hXFK0aXjLL2zXZpUa8HNDH0GJacsmbJ-DLDo2LBOiFLSgV9XCyoqHjZdbI6K56ltKWUcVnTp8UZb7tWyJYvil-fgwczex2J2eiozQQR7_SEYSR6tHkmPDlpT9KcDOwm7NHjtCfBkcnYS1KOsM6SWyArH9IU0eI8EIvOoUEPBFPweoJEXAwDuZ71uL7bhPmCrDY46ufFE6d9ghcnPC--X334tvpY3ny5_rS6vClNU3VT2btetBZA17VwYDonpOCOOesorduGsdZKJ9q2ll3PrO4462Vb1cAaoZ1uZXVevD7u3cXwc4Y0qQHzOd7rEcKcFBNdw2suOX8AVVQdFZLWmfr2SM0ZpRTBqV3EQce9YlQd6lJb9W9d6lCXYlxlyOKXJ5-5H8D-lf7pJxPeHwmQg7lFiCoZhNGAxQhmUjbgw3ze_bfGeBzRaP8D9pC2YY5jjl4xlbJAfT08zuFvWHOfbV39Bo0uxPc</recordid><startdate>20160401</startdate><enddate>20160401</enddate><creator>Lidan, Chen</creator><creator>Linhai, Li</creator><creator>Yang, Liao</creator><creator>Zhaohui, Sun</creator><creator>Xiaoyan, Huang</creator><creator>Yuling, Shi</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20160401</creationdate><title>Molecular characterization and antimicrobial susceptibility of tcdA -negative Clostridium difficile isolates from Guangzhou, China</title><author>Lidan, Chen ; Linhai, Li ; Yang, Liao ; Zhaohui, Sun ; Xiaoyan, Huang ; Yuling, Shi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c538t-bfb76deea447fec8f7972f1fdf00465116d9f766498b1da821b9634e157afa693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adult</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antimicrobial susceptibility test</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Toxins - genetics</topic><topic>China</topic><topic>Clostridium difficile</topic><topic>Clostridium difficile - classification</topic><topic>Clostridium difficile - drug effects</topic><topic>Clostridium difficile - genetics</topic><topic>Clostridium difficile - isolation & purification</topic><topic>Clostridium Infections - microbiology</topic><topic>Drug Resistance, Bacterial</topic><topic>Enterotoxins - deficiency</topic><topic>Genetic Variation</topic><topic>Genome, Bacterial</topic><topic>Genotype</topic><topic>gyrA gene</topic><topic>gyrB gene</topic><topic>High-throughput sequencing</topic><topic>Hospitals, General</topic><topic>Humans</topic><topic>Infectious Disease</topic><topic>Internal Medicine</topic><topic>Microbial Sensitivity Tests</topic><topic>Sequence Analysis, DNA</topic><topic>TcdA-negative strain</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lidan, Chen</creatorcontrib><creatorcontrib>Linhai, Li</creatorcontrib><creatorcontrib>Yang, Liao</creatorcontrib><creatorcontrib>Zhaohui, Sun</creatorcontrib><creatorcontrib>Xiaoyan, Huang</creatorcontrib><creatorcontrib>Yuling, Shi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Diagnostic microbiology and infectious disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lidan, Chen</au><au>Linhai, Li</au><au>Yang, Liao</au><au>Zhaohui, Sun</au><au>Xiaoyan, Huang</au><au>Yuling, Shi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular characterization and antimicrobial susceptibility of tcdA -negative Clostridium difficile isolates from Guangzhou, China</atitle><jtitle>Diagnostic microbiology and infectious disease</jtitle><addtitle>Diagn Microbiol Infect Dis</addtitle><date>2016-04-01</date><risdate>2016</risdate><volume>84</volume><issue>4</issue><spage>361</spage><epage>365</epage><pages>361-365</pages><issn>0732-8893</issn><eissn>1879-0070</eissn><abstract>Abstract This study aimed to investigate the molecular characteristics and antimicrobial susceptibility of Clostridium difficile clinical isolates in Guangzhou, China. One hundred twenty isolates were collected from Guangzhou General Hospital at the Guangzhou Military Command in China from March 2014 to April 2015, and 9 isolates were identified as tcdA -negative/ tcdB -positive (A− B+ ) strains. Results showed that all of the strains were confirmed to be ST37 and 0 single nucleotide variants (SNVs) were found in the PaLoc region, and >60 SNVs were identified throughout the whole genome sequence. The results show the diversity of the antibiotic and gene mutations present in these strains. All of the A− B+ isolates were highly resistant to clindamycin and erythromycin; showed an average sensitivity to fluoroquinolones; and maintained a high susceptibility to metronidazole, vancomycin, and tigecycline.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>26867962</pmid><doi>10.1016/j.diagmicrobio.2015.12.015</doi><tpages>5</tpages></addata></record> |
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| subjects | Adult Anti-Bacterial Agents - pharmacology Antimicrobial susceptibility test Bacterial Proteins - genetics Bacterial Toxins - genetics China Clostridium difficile Clostridium difficile - classification Clostridium difficile - drug effects Clostridium difficile - genetics Clostridium difficile - isolation & purification Clostridium Infections - microbiology Drug Resistance, Bacterial Enterotoxins - deficiency Genetic Variation Genome, Bacterial Genotype gyrA gene gyrB gene High-throughput sequencing Hospitals, General Humans Infectious Disease Internal Medicine Microbial Sensitivity Tests Sequence Analysis, DNA TcdA-negative strain |
| title | Molecular characterization and antimicrobial susceptibility of tcdA -negative Clostridium difficile isolates from Guangzhou, China |
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