Glycogen storage in the human retinal pigment epithelium: a comparative study of diabetic and non-diabetic donors
Liver and muscle glycogen content is reduced in diabetic patients but there is no information on the effect of diabetes on the glycogen content in the retinal pigment epithelium (RPE). The main aim of the study was to compare the glycogen content in the RPE between diabetic and non-diabetic human do...
Gespeichert in:
| Veröffentlicht in: | Acta diabetologica 2014-08, Vol.51 (4), p.543-552 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 552 |
|---|---|
| container_issue | 4 |
| container_start_page | 543 |
| container_title | Acta diabetologica |
| container_volume | 51 |
| creator | Hernández, Cristina Garcia-Ramírez, Marta García-Rocha, Mar Saez-López, Cristina Valverde, Ángela M. Guinovart, Joan J. Simó, Rafael |
| description | Liver and muscle glycogen content is reduced in diabetic patients but there is no information on the effect of diabetes on the glycogen content in the retinal pigment epithelium (RPE). The main aim of the study was to compare the glycogen content in the RPE between diabetic and non-diabetic human donors. Glycogen synthase (GS) and glycogen phosphorylase (GP), the key enzymes of glycogen metabolism, as well as their isoforms, were also assessed. For this purpose, 44 human postmortem eye cups were included (22 from 11 type 2 diabetic and 22 from 11 non-diabetic donors matched by age). Human RPE cells cultured in normoglycemic and hyperglycemic conditions were also analyzed. Glycogen content as well as the mRNA, protein content and enzyme activity of GS and GP were determined. In addition, GS and GP isoforms were characterized. In the RPE from diabetic donors, as well as in RPE cells grown in hyperglycemic conditions, the glycogen content was increased. The increase in glycogen content was associated with an increase in GS without changes in GP levels. In RPE form human donors, the muscle GS isoform but not the liver GS isoform was detected. Regarding GP, the muscle and brain isoform of GP but not the liver GP isoform were detected. We conclude that glycogen storage is increased in the RPE of diabetic patients, and it is associated with an increase in GS activity. Further studies aimed at determining the role of glycogen deposits in the pathogenesis of diabetic retinopathy are warranted. |
| doi_str_mv | 10.1007/s00592-013-0549-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1785234597</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1785234597</sourcerecordid><originalsourceid>FETCH-LOGICAL-c475t-ff594ffefaad90903e2f367e7a7c8f542dbd6d4cdccd04cf9dc776732902263b3</originalsourceid><addsrcrecordid>eNqFkU1LHTEUhkOx1KvtD-imBLrpJm0-b5LuRKoWhG7adcjNxzUyk4zJTOH-eyOjUoTiKpDzvG_IeQD4SPBXgrH81jAWmiJMGMKCa6TegA3hjCJBGTsCG6w5RoJTfQxOWrvFmFDJ1DtwTDkXSkuxAXeXw8GVfciwzaXafYApw_kmwJtltBnWMKdsBzil_RjyDMOU-nBIy_gdWujKONlq5_Q39PjiD7BE6JPd9ZSDNnuYS0bPF77kUtt78DbaoYUPj-cp-HPx4_f5Fbr-dfnz_OwaOS7FjGIUmscYorVeY41ZoJFtZZBWOhX7p_zObz133jmPuYvaOym3klGNKd2yHTsFX9beqZa7JbTZjKm5MAw2h7I0Q6Tqa-JCy9dRIajCSknR0c8v0Nuy1L6ilWJMEEU7RVbK1dJaDdFMNY22HgzB5kGdWdWZrs48qDOqZz49Ni-7MfjnxJOrDtAVaH2U96H-8_R_W-8BLeCk4w</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1552335182</pqid></control><display><type>article</type><title>Glycogen storage in the human retinal pigment epithelium: a comparative study of diabetic and non-diabetic donors</title><source>MEDLINE</source><source>Springer journals</source><creator>Hernández, Cristina ; Garcia-Ramírez, Marta ; García-Rocha, Mar ; Saez-López, Cristina ; Valverde, Ángela M. ; Guinovart, Joan J. ; Simó, Rafael</creator><creatorcontrib>Hernández, Cristina ; Garcia-Ramírez, Marta ; García-Rocha, Mar ; Saez-López, Cristina ; Valverde, Ángela M. ; Guinovart, Joan J. ; Simó, Rafael</creatorcontrib><description>Liver and muscle glycogen content is reduced in diabetic patients but there is no information on the effect of diabetes on the glycogen content in the retinal pigment epithelium (RPE). The main aim of the study was to compare the glycogen content in the RPE between diabetic and non-diabetic human donors. Glycogen synthase (GS) and glycogen phosphorylase (GP), the key enzymes of glycogen metabolism, as well as their isoforms, were also assessed. For this purpose, 44 human postmortem eye cups were included (22 from 11 type 2 diabetic and 22 from 11 non-diabetic donors matched by age). Human RPE cells cultured in normoglycemic and hyperglycemic conditions were also analyzed. Glycogen content as well as the mRNA, protein content and enzyme activity of GS and GP were determined. In addition, GS and GP isoforms were characterized. In the RPE from diabetic donors, as well as in RPE cells grown in hyperglycemic conditions, the glycogen content was increased. The increase in glycogen content was associated with an increase in GS without changes in GP levels. In RPE form human donors, the muscle GS isoform but not the liver GS isoform was detected. Regarding GP, the muscle and brain isoform of GP but not the liver GP isoform were detected. We conclude that glycogen storage is increased in the RPE of diabetic patients, and it is associated with an increase in GS activity. Further studies aimed at determining the role of glycogen deposits in the pathogenesis of diabetic retinopathy are warranted.</description><identifier>ISSN: 0940-5429</identifier><identifier>EISSN: 1432-5233</identifier><identifier>DOI: 10.1007/s00592-013-0549-8</identifier><identifier>PMID: 24458975</identifier><identifier>CODEN: ACDAEZ</identifier><language>eng</language><publisher>Milan: Springer Milan</publisher><subject>Aged ; Aged, 80 and over ; Blood & organ donations ; Comparative studies ; Diabetes ; Diabetes Mellitus - enzymology ; Diabetes Mellitus - genetics ; Diabetes Mellitus - metabolism ; Female ; Glycogen - metabolism ; Glycogen Phosphorylase - genetics ; Glycogen Phosphorylase - metabolism ; Glycogen Synthase - genetics ; Glycogen Synthase - metabolism ; Humans ; Internal Medicine ; Liver - enzymology ; Liver - metabolism ; Male ; Medicine ; Medicine & Public Health ; Metabolic Diseases ; Middle Aged ; Muscle, Skeletal - enzymology ; Muscle, Skeletal - metabolism ; Original Article ; Pigments ; Retina ; Retinal Pigment Epithelium - enzymology ; Retinal Pigment Epithelium - metabolism ; Tissue Donors</subject><ispartof>Acta diabetologica, 2014-08, Vol.51 (4), p.543-552</ispartof><rights>Springer-Verlag Italia 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-ff594ffefaad90903e2f367e7a7c8f542dbd6d4cdccd04cf9dc776732902263b3</citedby><cites>FETCH-LOGICAL-c475t-ff594ffefaad90903e2f367e7a7c8f542dbd6d4cdccd04cf9dc776732902263b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00592-013-0549-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00592-013-0549-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24458975$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hernández, Cristina</creatorcontrib><creatorcontrib>Garcia-Ramírez, Marta</creatorcontrib><creatorcontrib>García-Rocha, Mar</creatorcontrib><creatorcontrib>Saez-López, Cristina</creatorcontrib><creatorcontrib>Valverde, Ángela M.</creatorcontrib><creatorcontrib>Guinovart, Joan J.</creatorcontrib><creatorcontrib>Simó, Rafael</creatorcontrib><title>Glycogen storage in the human retinal pigment epithelium: a comparative study of diabetic and non-diabetic donors</title><title>Acta diabetologica</title><addtitle>Acta Diabetol</addtitle><addtitle>Acta Diabetol</addtitle><description>Liver and muscle glycogen content is reduced in diabetic patients but there is no information on the effect of diabetes on the glycogen content in the retinal pigment epithelium (RPE). The main aim of the study was to compare the glycogen content in the RPE between diabetic and non-diabetic human donors. Glycogen synthase (GS) and glycogen phosphorylase (GP), the key enzymes of glycogen metabolism, as well as their isoforms, were also assessed. For this purpose, 44 human postmortem eye cups were included (22 from 11 type 2 diabetic and 22 from 11 non-diabetic donors matched by age). Human RPE cells cultured in normoglycemic and hyperglycemic conditions were also analyzed. Glycogen content as well as the mRNA, protein content and enzyme activity of GS and GP were determined. In addition, GS and GP isoforms were characterized. In the RPE from diabetic donors, as well as in RPE cells grown in hyperglycemic conditions, the glycogen content was increased. The increase in glycogen content was associated with an increase in GS without changes in GP levels. In RPE form human donors, the muscle GS isoform but not the liver GS isoform was detected. Regarding GP, the muscle and brain isoform of GP but not the liver GP isoform were detected. We conclude that glycogen storage is increased in the RPE of diabetic patients, and it is associated with an increase in GS activity. Further studies aimed at determining the role of glycogen deposits in the pathogenesis of diabetic retinopathy are warranted.</description><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Blood & organ donations</subject><subject>Comparative studies</subject><subject>Diabetes</subject><subject>Diabetes Mellitus - enzymology</subject><subject>Diabetes Mellitus - genetics</subject><subject>Diabetes Mellitus - metabolism</subject><subject>Female</subject><subject>Glycogen - metabolism</subject><subject>Glycogen Phosphorylase - genetics</subject><subject>Glycogen Phosphorylase - metabolism</subject><subject>Glycogen Synthase - genetics</subject><subject>Glycogen Synthase - metabolism</subject><subject>Humans</subject><subject>Internal Medicine</subject><subject>Liver - enzymology</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Metabolic Diseases</subject><subject>Middle Aged</subject><subject>Muscle, Skeletal - enzymology</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Original Article</subject><subject>Pigments</subject><subject>Retina</subject><subject>Retinal Pigment Epithelium - enzymology</subject><subject>Retinal Pigment Epithelium - metabolism</subject><subject>Tissue Donors</subject><issn>0940-5429</issn><issn>1432-5233</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkU1LHTEUhkOx1KvtD-imBLrpJm0-b5LuRKoWhG7adcjNxzUyk4zJTOH-eyOjUoTiKpDzvG_IeQD4SPBXgrH81jAWmiJMGMKCa6TegA3hjCJBGTsCG6w5RoJTfQxOWrvFmFDJ1DtwTDkXSkuxAXeXw8GVfciwzaXafYApw_kmwJtltBnWMKdsBzil_RjyDMOU-nBIy_gdWujKONlq5_Q39PjiD7BE6JPd9ZSDNnuYS0bPF77kUtt78DbaoYUPj-cp-HPx4_f5Fbr-dfnz_OwaOS7FjGIUmscYorVeY41ZoJFtZZBWOhX7p_zObz133jmPuYvaOym3klGNKd2yHTsFX9beqZa7JbTZjKm5MAw2h7I0Q6Tqa-JCy9dRIajCSknR0c8v0Nuy1L6ilWJMEEU7RVbK1dJaDdFMNY22HgzB5kGdWdWZrs48qDOqZz49Ni-7MfjnxJOrDtAVaH2U96H-8_R_W-8BLeCk4w</recordid><startdate>20140801</startdate><enddate>20140801</enddate><creator>Hernández, Cristina</creator><creator>Garcia-Ramírez, Marta</creator><creator>García-Rocha, Mar</creator><creator>Saez-López, Cristina</creator><creator>Valverde, Ángela M.</creator><creator>Guinovart, Joan J.</creator><creator>Simó, Rafael</creator><general>Springer Milan</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20140801</creationdate><title>Glycogen storage in the human retinal pigment epithelium: a comparative study of diabetic and non-diabetic donors</title><author>Hernández, Cristina ; Garcia-Ramírez, Marta ; García-Rocha, Mar ; Saez-López, Cristina ; Valverde, Ángela M. ; Guinovart, Joan J. ; Simó, Rafael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-ff594ffefaad90903e2f367e7a7c8f542dbd6d4cdccd04cf9dc776732902263b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Blood & organ donations</topic><topic>Comparative studies</topic><topic>Diabetes</topic><topic>Diabetes Mellitus - enzymology</topic><topic>Diabetes Mellitus - genetics</topic><topic>Diabetes Mellitus - metabolism</topic><topic>Female</topic><topic>Glycogen - metabolism</topic><topic>Glycogen Phosphorylase - genetics</topic><topic>Glycogen Phosphorylase - metabolism</topic><topic>Glycogen Synthase - genetics</topic><topic>Glycogen Synthase - metabolism</topic><topic>Humans</topic><topic>Internal Medicine</topic><topic>Liver - enzymology</topic><topic>Liver - metabolism</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Metabolic Diseases</topic><topic>Middle Aged</topic><topic>Muscle, Skeletal - enzymology</topic><topic>Muscle, Skeletal - metabolism</topic><topic>Original Article</topic><topic>Pigments</topic><topic>Retina</topic><topic>Retinal Pigment Epithelium - enzymology</topic><topic>Retinal Pigment Epithelium - metabolism</topic><topic>Tissue Donors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hernández, Cristina</creatorcontrib><creatorcontrib>Garcia-Ramírez, Marta</creatorcontrib><creatorcontrib>García-Rocha, Mar</creatorcontrib><creatorcontrib>Saez-López, Cristina</creatorcontrib><creatorcontrib>Valverde, Ángela M.</creatorcontrib><creatorcontrib>Guinovart, Joan J.</creatorcontrib><creatorcontrib>Simó, Rafael</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>ProQuest_Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest_Research Library</collection><collection>Research Library (Corporate)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Acta diabetologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hernández, Cristina</au><au>Garcia-Ramírez, Marta</au><au>García-Rocha, Mar</au><au>Saez-López, Cristina</au><au>Valverde, Ángela M.</au><au>Guinovart, Joan J.</au><au>Simó, Rafael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Glycogen storage in the human retinal pigment epithelium: a comparative study of diabetic and non-diabetic donors</atitle><jtitle>Acta diabetologica</jtitle><stitle>Acta Diabetol</stitle><addtitle>Acta Diabetol</addtitle><date>2014-08-01</date><risdate>2014</risdate><volume>51</volume><issue>4</issue><spage>543</spage><epage>552</epage><pages>543-552</pages><issn>0940-5429</issn><eissn>1432-5233</eissn><coden>ACDAEZ</coden><abstract>Liver and muscle glycogen content is reduced in diabetic patients but there is no information on the effect of diabetes on the glycogen content in the retinal pigment epithelium (RPE). The main aim of the study was to compare the glycogen content in the RPE between diabetic and non-diabetic human donors. Glycogen synthase (GS) and glycogen phosphorylase (GP), the key enzymes of glycogen metabolism, as well as their isoforms, were also assessed. For this purpose, 44 human postmortem eye cups were included (22 from 11 type 2 diabetic and 22 from 11 non-diabetic donors matched by age). Human RPE cells cultured in normoglycemic and hyperglycemic conditions were also analyzed. Glycogen content as well as the mRNA, protein content and enzyme activity of GS and GP were determined. In addition, GS and GP isoforms were characterized. In the RPE from diabetic donors, as well as in RPE cells grown in hyperglycemic conditions, the glycogen content was increased. The increase in glycogen content was associated with an increase in GS without changes in GP levels. In RPE form human donors, the muscle GS isoform but not the liver GS isoform was detected. Regarding GP, the muscle and brain isoform of GP but not the liver GP isoform were detected. We conclude that glycogen storage is increased in the RPE of diabetic patients, and it is associated with an increase in GS activity. Further studies aimed at determining the role of glycogen deposits in the pathogenesis of diabetic retinopathy are warranted.</abstract><cop>Milan</cop><pub>Springer Milan</pub><pmid>24458975</pmid><doi>10.1007/s00592-013-0549-8</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0940-5429 |
| ispartof | Acta diabetologica, 2014-08, Vol.51 (4), p.543-552 |
| issn | 0940-5429 1432-5233 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1785234597 |
| source | MEDLINE; Springer journals |
| subjects | Aged Aged, 80 and over Blood & organ donations Comparative studies Diabetes Diabetes Mellitus - enzymology Diabetes Mellitus - genetics Diabetes Mellitus - metabolism Female Glycogen - metabolism Glycogen Phosphorylase - genetics Glycogen Phosphorylase - metabolism Glycogen Synthase - genetics Glycogen Synthase - metabolism Humans Internal Medicine Liver - enzymology Liver - metabolism Male Medicine Medicine & Public Health Metabolic Diseases Middle Aged Muscle, Skeletal - enzymology Muscle, Skeletal - metabolism Original Article Pigments Retina Retinal Pigment Epithelium - enzymology Retinal Pigment Epithelium - metabolism Tissue Donors |
| title | Glycogen storage in the human retinal pigment epithelium: a comparative study of diabetic and non-diabetic donors |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T14%3A39%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Glycogen%20storage%20in%20the%20human%20retinal%20pigment%20epithelium:%20a%20comparative%20study%20of%20diabetic%20and%20non-diabetic%20donors&rft.jtitle=Acta%20diabetologica&rft.au=Hern%C3%A1ndez,%20Cristina&rft.date=2014-08-01&rft.volume=51&rft.issue=4&rft.spage=543&rft.epage=552&rft.pages=543-552&rft.issn=0940-5429&rft.eissn=1432-5233&rft.coden=ACDAEZ&rft_id=info:doi/10.1007/s00592-013-0549-8&rft_dat=%3Cproquest_cross%3E1785234597%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1552335182&rft_id=info:pmid/24458975&rfr_iscdi=true |