Performance evaluation of the Sysmex XN-1000 hematology analyzer in assessment of the white blood cell count differential in pediatric specimens
Introduction The automated XN‐1000 hematology analyzer enables to perform a blood cell count and a leukocyte differential. When abnormal cells were detected, a flag was generated by the analyzer and a manual microscopic examination of the corresponding blood film was performed. Methods We compared t...
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| Veröffentlicht in: | International journal of laboratory hematology 2016-02, Vol.38 (1), p.54-63 |
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| container_title | International journal of laboratory hematology |
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| creator | Becker, P.-H. Fenneteau, O. Da Costa, L. |
| description | Introduction
The automated XN‐1000 hematology analyzer enables to perform a blood cell count and a leukocyte differential. When abnormal cells were detected, a flag was generated by the analyzer and a manual microscopic examination of the corresponding blood film was performed.
Methods
We compared the white blood cell differentials provided by the automated hematology analyzer XN‐1000 in a pediatric population (n = 765) with those obtained through microscopic examination by cytologists and those obtained using a previous version of this analyzer, the XE‐2100. Leukocytes count as well as flags sensitivity and specificity was analyzed.
Results
The leukocytes count provided by the analyzer is in good accordance with the differential obtained by manual count in children older than 3 months. The sensitivity for blast detection is 99% and the detection of reactive cells is 63%. The flag specificity remains low ( |
| doi_str_mv | 10.1111/ijlh.12436 |
| format | Article |
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The automated XN‐1000 hematology analyzer enables to perform a blood cell count and a leukocyte differential. When abnormal cells were detected, a flag was generated by the analyzer and a manual microscopic examination of the corresponding blood film was performed.
Methods
We compared the white blood cell differentials provided by the automated hematology analyzer XN‐1000 in a pediatric population (n = 765) with those obtained through microscopic examination by cytologists and those obtained using a previous version of this analyzer, the XE‐2100. Leukocytes count as well as flags sensitivity and specificity was analyzed.
Results
The leukocytes count provided by the analyzer is in good accordance with the differential obtained by manual count in children older than 3 months. The sensitivity for blast detection is 99% and the detection of reactive cells is 63%. The flag specificity remains low (<35%) for blood samples collected from infants between 8 days and 2 years of age, but increases up to 67% thereafter. The results obtained with the XN‐1000 analyzer show an improvement in comparison with those obtained with the XE‐2100 analyzer.
Conclusion
The automated WBC differential provided by the XN‐1000 analyzer in the pediatric setting is accurate, but a meticulous microscopic examination of blood smears remains necessary for infants up to 3 months of age to validate the analyzer flags.</description><identifier>ISSN: 1751-5521</identifier><identifier>EISSN: 1751-553X</identifier><identifier>DOI: 10.1111/ijlh.12436</identifier><identifier>PMID: 26407903</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Adolescent ; Automated blood cells analyzers ; Automation, Laboratory ; Child ; Child, Preschool ; Female ; flags ; Humans ; Infant ; Infant, Newborn ; Leukocyte Count - instrumentation ; Leukocyte Count - methods ; Leukocyte Count - standards ; Leukocytes - cytology ; Leukocytes - pathology ; Male ; Microscopy - methods ; pediatrics ; Reproducibility of Results ; Sensitivity and Specificity ; Sysmex XN-1000 ; WBC differentials</subject><ispartof>International journal of laboratory hematology, 2016-02, Vol.38 (1), p.54-63</ispartof><rights>2015 John Wiley & Sons Ltd</rights><rights>2015 John Wiley & Sons Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4706-4e089febb7e47b14946a0a14438fa08d3a7864314b582b2c4be9dd1623a718343</citedby><cites>FETCH-LOGICAL-c4706-4e089febb7e47b14946a0a14438fa08d3a7864314b582b2c4be9dd1623a718343</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fijlh.12436$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fijlh.12436$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26407903$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Becker, P.-H.</creatorcontrib><creatorcontrib>Fenneteau, O.</creatorcontrib><creatorcontrib>Da Costa, L.</creatorcontrib><title>Performance evaluation of the Sysmex XN-1000 hematology analyzer in assessment of the white blood cell count differential in pediatric specimens</title><title>International journal of laboratory hematology</title><addtitle>Int. Jnl. Lab. Hem</addtitle><description>Introduction
The automated XN‐1000 hematology analyzer enables to perform a blood cell count and a leukocyte differential. When abnormal cells were detected, a flag was generated by the analyzer and a manual microscopic examination of the corresponding blood film was performed.
Methods
We compared the white blood cell differentials provided by the automated hematology analyzer XN‐1000 in a pediatric population (n = 765) with those obtained through microscopic examination by cytologists and those obtained using a previous version of this analyzer, the XE‐2100. Leukocytes count as well as flags sensitivity and specificity was analyzed.
Results
The leukocytes count provided by the analyzer is in good accordance with the differential obtained by manual count in children older than 3 months. The sensitivity for blast detection is 99% and the detection of reactive cells is 63%. The flag specificity remains low (<35%) for blood samples collected from infants between 8 days and 2 years of age, but increases up to 67% thereafter. The results obtained with the XN‐1000 analyzer show an improvement in comparison with those obtained with the XE‐2100 analyzer.
Conclusion
The automated WBC differential provided by the XN‐1000 analyzer in the pediatric setting is accurate, but a meticulous microscopic examination of blood smears remains necessary for infants up to 3 months of age to validate the analyzer flags.</description><subject>Adolescent</subject><subject>Automated blood cells analyzers</subject><subject>Automation, Laboratory</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Female</subject><subject>flags</subject><subject>Humans</subject><subject>Infant</subject><subject>Infant, Newborn</subject><subject>Leukocyte Count - instrumentation</subject><subject>Leukocyte Count - methods</subject><subject>Leukocyte Count - standards</subject><subject>Leukocytes - cytology</subject><subject>Leukocytes - pathology</subject><subject>Male</subject><subject>Microscopy - methods</subject><subject>pediatrics</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Sysmex XN-1000</subject><subject>WBC differentials</subject><issn>1751-5521</issn><issn>1751-553X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAURi1ERcvAhgdAXqJKKXbsxMkSVfQHDQWpLZ2ddZPcMC5OPNgJbXiKPnI9nc4sEV7Ylu75jix_hLzj7IjH9dHc2uURT6XIX5ADrjKeZJlYvNzdU75PXodwy1imJCtfkf00l0yVTByQh-_oW-c76Guk-AfsCINxPXUtHZZIL6fQ4T1dXCScMUaX2MHgrPs5UejBTn_RU9NTCAFDBPthm7tbmgFpZZ1raI3W0tqNcdqYtkUfOQN2HVxhY2DwpqZhhbWJhvCG7LVgA759Pmfk-uTz1fFZMv92en78aZ7UUrE8kciKssWqUihVxWUpc2DApRRFC6xoBKgil4LLKivSKq1lhWXT8DyNA14IKWbkw8a78u73iGHQnQnrp0KPbgyaqyJLhZAs-w80Z2Xc4ofOyOEGrb0LwWOrV9504CfNmV6Xpddl6aeyIvz-2TtWHTY7dNtOBPgGuDMWp3-o9PmX-dlWmmwyJgx4v8uA_6VzJVSmby5O9debxaWcc6l_iEcPo69Z</recordid><startdate>201602</startdate><enddate>201602</enddate><creator>Becker, P.-H.</creator><creator>Fenneteau, O.</creator><creator>Da Costa, L.</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>201602</creationdate><title>Performance evaluation of the Sysmex XN-1000 hematology analyzer in assessment of the white blood cell count differential in pediatric specimens</title><author>Becker, P.-H. ; Fenneteau, O. ; Da Costa, L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4706-4e089febb7e47b14946a0a14438fa08d3a7864314b582b2c4be9dd1623a718343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adolescent</topic><topic>Automated blood cells analyzers</topic><topic>Automation, Laboratory</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Female</topic><topic>flags</topic><topic>Humans</topic><topic>Infant</topic><topic>Infant, Newborn</topic><topic>Leukocyte Count - instrumentation</topic><topic>Leukocyte Count - methods</topic><topic>Leukocyte Count - standards</topic><topic>Leukocytes - cytology</topic><topic>Leukocytes - pathology</topic><topic>Male</topic><topic>Microscopy - methods</topic><topic>pediatrics</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Sysmex XN-1000</topic><topic>WBC differentials</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Becker, P.-H.</creatorcontrib><creatorcontrib>Fenneteau, O.</creatorcontrib><creatorcontrib>Da Costa, L.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>International journal of laboratory hematology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Becker, P.-H.</au><au>Fenneteau, O.</au><au>Da Costa, L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Performance evaluation of the Sysmex XN-1000 hematology analyzer in assessment of the white blood cell count differential in pediatric specimens</atitle><jtitle>International journal of laboratory hematology</jtitle><addtitle>Int. Jnl. Lab. Hem</addtitle><date>2016-02</date><risdate>2016</risdate><volume>38</volume><issue>1</issue><spage>54</spage><epage>63</epage><pages>54-63</pages><issn>1751-5521</issn><eissn>1751-553X</eissn><abstract>Introduction
The automated XN‐1000 hematology analyzer enables to perform a blood cell count and a leukocyte differential. When abnormal cells were detected, a flag was generated by the analyzer and a manual microscopic examination of the corresponding blood film was performed.
Methods
We compared the white blood cell differentials provided by the automated hematology analyzer XN‐1000 in a pediatric population (n = 765) with those obtained through microscopic examination by cytologists and those obtained using a previous version of this analyzer, the XE‐2100. Leukocytes count as well as flags sensitivity and specificity was analyzed.
Results
The leukocytes count provided by the analyzer is in good accordance with the differential obtained by manual count in children older than 3 months. The sensitivity for blast detection is 99% and the detection of reactive cells is 63%. The flag specificity remains low (<35%) for blood samples collected from infants between 8 days and 2 years of age, but increases up to 67% thereafter. The results obtained with the XN‐1000 analyzer show an improvement in comparison with those obtained with the XE‐2100 analyzer.
Conclusion
The automated WBC differential provided by the XN‐1000 analyzer in the pediatric setting is accurate, but a meticulous microscopic examination of blood smears remains necessary for infants up to 3 months of age to validate the analyzer flags.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>26407903</pmid><doi>10.1111/ijlh.12436</doi><tpages>10</tpages></addata></record> |
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| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Adolescent Automated blood cells analyzers Automation, Laboratory Child Child, Preschool Female flags Humans Infant Infant, Newborn Leukocyte Count - instrumentation Leukocyte Count - methods Leukocyte Count - standards Leukocytes - cytology Leukocytes - pathology Male Microscopy - methods pediatrics Reproducibility of Results Sensitivity and Specificity Sysmex XN-1000 WBC differentials |
| title | Performance evaluation of the Sysmex XN-1000 hematology analyzer in assessment of the white blood cell count differential in pediatric specimens |
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